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    • 4. 发明申请
    • Detection of biological molecules using THz absorption spectroscopy
    • 使用THz吸收光谱法检测生物分子
    • US20080014580A1
    • 2008-01-17
    • US10827974
    • 2004-04-19
    • Robert R. AlfanoBaolong YuYuanlong Yang
    • Robert R. AlfanoBaolong YuYuanlong Yang
    • C12Q1/68G01N21/00
    • G01N21/3586
    • A method and apparatus of detecting biological molecules, the method including the steps of: performing Terahertz (THz) absorption spectroscopy, performed in a first frequency range of 0.2 to 2.2 THz (10-79.2 cm−1), on at least one sample including a substance comprising the biological molecules, the substance being selected from at least one of tryptophan, albumin bovine, DNA, nucleotides, bacillus subtilis, spore, and DPA; calculating a frequency-dependent absorption value of biological molecules; performing THz absorption spectroscopy on at least one reference substance; detecting the substance through the frequency-dependent absorption value by comparison of absorption peaks; and outputting information proving existence of the substance in the sample. The method further creates a library of known THz frequency modes on spectra to identify the presence of unknown substance in biological and chemical composite media.
    • 一种检测生物分子的方法和装置,所述方法包括以下步骤:在至少一个样品上进行在0.2至2.2THz(10-79.2cm-1)的第一频率范围内执行的太赫兹(THz)吸收光谱,包括 包含生物分子的物质,该物质选自色氨酸,白蛋白牛,DNA,核苷酸,枯草杆菌,孢子和DPA中的至少一种; 计算生物分子的频率依赖吸收值; 对至少一种参考物质进行太赫兹吸收光谱; 通过吸收峰的比较通过频率依赖吸收值检测物质; 并输出证明样品中物质存在的信息。 该方法进一步在光谱上创建已知的THz频率模式的文库,以识别生物和化学复合介质中未知物质的存在。
    • 6. 发明授权
    • Technique for examining biological materials using diffuse reflectance spectroscopy and the kubelka-munk function
    • 使用漫反射光谱和kubelka-munk功能检查生物材料的技术
    • US06615068B1
    • 2003-09-02
    • US09598007
    • 2000-06-19
    • Robert R. AlfanoYuanlong Yang
    • Robert R. AlfanoYuanlong Yang
    • A61B500
    • A61B5/444A61B5/0059A61B5/0075A61B5/0091G01N21/314
    • Method and apparatus for examining biological materials using diffuse reflectance spectroscopy and the Kubelka-Munk function. In one aspect, the method is used to determine whether a tissue sample is cancerous or not and comprises the steps of (a) measuring the diffuse reflectance from the tissue sample at a first wavelength and at a second wavelength, wherein the first wavelength is a wavelength selected from the group consisting of 255-265 nm and wherein the second wavelength is a wavelength selected from the group consisting of 275-285 nm; (b) using the Kubelka-Munk function to transform the diffuse reflectance measurement obtained at the first and second wavelengths; and (c) comparing a ratio or a difference of the transformed Kubelka-Munk measurements at the first and second wavelengths to appropriate standards determine whether or not the tissue sample is cancerous. One can use the spectral profile of KMF between 250 nm to 300 nm to determine whether or not the tissue sample is cancerous or precancerous. According to the value at the first and second wavelengths determine whether or not the malignant tissue is invasive or mixed invasive and in situ or carcinoma in situ.
    • 使用漫反射光谱和Kubelka-Munk功能检测生物材料的方法和装置。 在一个方面,该方法用于确定组织样品是否是癌变的,并且包括以下步骤:(a)测量来自第一波长和第二波长的组织样品的漫反射率,其中第一波长为 选自255-265nm的波长,其中第二波长是选自275-285nm的波长; (b)使用Kubelka-Munk函数来变换在第一和第二波长处获得的漫反射测量值; 和(c)将在第一和第二波长处的变换的Kubelka-Munk测量值的比率或差异与适当的标准进行比较,确定组织样品是否是癌变的。 可以使用KMF的光谱轮廓在250nm至300nm之间来确定组织样品是否是癌性的或癌前的。 根据第一和第二波长的值确定恶性组织是侵入性还是侵入性原位或原位癌。