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    • 4. 发明申请
    • DETECTION METHOD OF NUCLEIC ACID AND KIT AND USING THEREOF
    • 核酸和试剂盒的检测方法及其使用
    • US20140017671A1
    • 2014-01-16
    • US14007201
    • 2011-04-12
    • Xingjie LiangHua DengXiaowei Ma
    • Xingjie LiangHua DengXiaowei Ma
    • C12Q1/68
    • C12Q1/686C12Q1/6816C12Q1/689C12Q2565/107
    • A detection method of nucleic acid is provided. The method includes: providing nucleic acid to be tested, making the nucleic acid to be tested react under asymmetric PCR conditions with a pair of primers for target nucleic acid amplification, DNA polymerase, adenine deoxyribonucleotide, guanine deoxyribonucleotide, cytosine deoxyribonucleotide and thymidine deoxyribonucleotide in a PCR buffer solution, mixing the reaction product and liquid that contains probe molecules, and judging whether the nucleic acid to be tested contains the target nucleic acid by observing the obtained mixture color or color change. A kit is also provided that can be used in the nucleic acid detection by the said method. Application of the method and the kit in inspection and quarantine is also provided. The method is a quick and easy, sensitive and a specific detection method of nucleic acid with direct observation using naked eyes. The method does not need additional equipment.
    • 提供核酸的检测方法。 该方法包括:提供待测试的核酸,使待测试的核酸在不对称PCR条件下与靶核酸扩增的一对引物,DNA聚合酶,腺嘌呤脱氧核糖核苷酸,鸟嘌呤脱氧核糖核苷酸,胞嘧啶脱氧核糖核苷酸和胸苷脱氧核糖核苷酸反应 PCR缓冲溶液,混合含有探针分子的反应产物和液体,并通过观察获得的混合物颜色或颜色变化来判断待测核酸是否含有靶核酸。 还提供了可用于所述方法的核酸检测中的试剂盒。 还提供了该方法和试剂盒在检验检疫中的应用。 该方法是使用肉眼直观观察的核酸的快速,容易,灵敏和具体的检测方法。 该方法不需要额外的设备。
    • 9. 发明授权
    • Center backfire inner heat regenerative energy saving high efficiency furnace and tank integration reduction furnace system
    • 中心回火内热再生节能高效炉和水箱一体化还原炉系统
    • US08057735B2
    • 2011-11-15
    • US12425369
    • 2009-04-16
    • Xiao-Bao DengQi-Ming LiMike LiHua Deng
    • Xiao-Bao DengQi-Ming LiMike LiHua Deng
    • C21B9/14
    • C22B26/22C22B5/04
    • A center backfire inner heat regenerative energy saving high efficiency furnace and tank integration reduction furnace system comprises a furnace section and a heating section. The furnace section includes a reduction tank, a material inlet door, a magnesium crystal collecting machine, and a residue exhaust pipe. The reduction tank includes a tank body, a centre combustion room, and plural fume exhaust pipes. The material inlet door is connected to a material charging room between the center combustion room and the tank body, and the magnesium crystal collecting machine is provided with a crystal cover communicating with the material charging room, and a water recycling condensation equipment outside the crystal cover. The residue exhaust pipe is connected to the material charging room and provided with a residue exhaust door and a water dispersing heat separating cover. The heating section includes a burner connected to the center combustion room.
    • 中心回火内热再生节能高效炉和罐整合还原炉系统包括炉段和加热段。 炉段包括还原箱,进料门,镁晶体收集机和残渣排气管。 还原槽包括罐体,中心燃烧室和多个排烟管。 物料入口门连接到中心燃烧室和罐体之间的物料充电室,镁晶体收集机设有与充电室连通的晶体盖,水晶盖外的水回收冷凝设备 。 残渣排气管连接到充料室,并设有残渣排气门和水分散热分离盖。 加热部包括连接到中心燃烧室的燃烧器。
    • 10. 发明授权
    • Detection method of nucleic acid and kit and using thereof
    • 核酸和试剂盒的检测方法及其应用
    • US09404153B2
    • 2016-08-02
    • US14007201
    • 2011-04-12
    • Xingjie LiangHua DengXiaowei MaChan Li
    • Xingjie LiangHua DengXiaowei MaChan Li
    • C12Q1/68
    • C12Q1/686C12Q1/6816C12Q1/689C12Q2565/107
    • A detection method of nucleic acid is provided. The method includes: providing nucleic acid to be tested, making the nucleic acid to be tested react under asymmetric PCR conditions with a pair of primers for target nucleic acid amplification, DNA polymerase, adenine deoxyribonucleotide, guanine deoxyribonucleotide, cytosine deoxyribonucleotide and thymidine deoxyribonucleotide in a PCR buffer solution, mixing the reaction product and liquid that contains probe molecules, and judging whether the nucleic acid to be tested contains the target nucleic acid by observing the obtained mixture color or color change. A kit is also provided that can be used in the nucleic acid detection by the said method. Application of the method and the kit in inspection and quarantine is also provided. The method is a quick and easy, sensitive and a specific detection method of nucleic acid with direct observation using naked eyes. The method does not need additional equipment.
    • 提供核酸的检测方法。 该方法包括:提供待测试的核酸,使待测试的核酸在不对称PCR条件下与靶核酸扩增的一对引物,DNA聚合酶,腺嘌呤脱氧核糖核苷酸,鸟嘌呤脱氧核糖核苷酸,胞嘧啶脱氧核糖核苷酸和胸苷脱氧核糖核苷酸反应 PCR缓冲溶液,混合含有探针分子的反应产物和液体,并通过观察获得的混合物颜色或颜色变化来判断待测核酸是否含有靶核酸。 还提供了可用于所述方法的核酸检测中的试剂盒。 还提供了该方法和试剂盒在检验检疫中的应用。 该方法是使用肉眼直观观察的核酸的快速,容易,灵敏和具体的检测方法。 该方法不需要额外的设备。