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1. 发明申请

US20050164388A1 Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells and preconditioned cells 审中-公开
标题翻译：分离上皮细胞的方法，预处理细胞的方法，以及用上皮细胞和预处理细胞制备生物人造皮肤和真皮的方法
公开(公告)号：US20050164388A1
公开(公告)日：2005-07-28
申请号：US10258987
申请日：2001-11-06
申请人： Young-Sook Son , Hyun-Sook Park , Chun-Ho Kim , Hyun-Ju Kang , Chang-Hwan Kim , Youn-Young Kim , Young-Ju Choi , Su-Hyun Lee , Yong-Jae Gin
发明人： Young-Sook Son , Hyun-Sook Park , Chun-Ho Kim , Hyun-Ju Kang , Chang-Hwan Kim , Youn-Young Kim , Young-Ju Choi , Su-Hyun Lee , Yong-Jae Gin
IPC分类号： A61K35/12 , A61L27/00 , C12N5/071 , C12N5/08 , A61F2/10
CPC分类号： C12N5/0698 , A61K35/12 , C12N5/0629 , C12N2502/094 , C12N2502/1323 , C12N2502/28 , C12N2509/00 , C12N2509/10
摘要： A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylene-diamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be isolated by the method with increased yield, colony forming efficiency (CFE), and colony size. Also, the increased percentage of stem cells in isolated cells is advantageous in therapeutic tissue implantation by autologous or allogeneic transplantation. In skin cells preconditioned by the application of strain, cell division is facilitated, and the secretion of extracellular matrix components and growth factors and the activity of matrix metalloproteinases (MMPs) are improved. When preconditioned cells are implanted by autologous or allogeneic transplantation to heal a damaged tissue, the improved cell adhesion, mobility, and viability provides a biological adjustment effect against a variety of stresses or physical stimuli which the cells would undergo after implantation, with improved capability of integration into host tissue, thereby markedly improving the probability of success in skin grafting.
摘要翻译：一种利用磁力搅拌同时使用胰蛋白酶和乙二胺四乙酸（EDTA）从人皮肤组织或内脏器官组织分离上皮细胞的方法，一种通过应用物理刺激预处理分离的生物细胞的方法，应变。可以通过增加产量，菌落形成效率（CFE）和菌落大小的方法分离上皮细胞。此外，分离的细胞中干细胞的增加百分比在通过自体或同种异体移植治疗组织植入方面是有利的。在通过应用菌株预处理的皮肤细胞中，促进细胞分裂，细胞外基质成分和生长因子的分泌以及基质金属蛋白酶（MMPs）的活性得到改善。当通过自体或同种异体移植植入预处理细胞以愈合损伤的组织时，改善的细胞粘附性，迁移性和活力提供了针对细胞在植入后将经历的各种应力或物理刺激的生物调节作用，具有改善的整合到宿主组织中，从而显着提高皮肤移植成功的可能性。

2. 发明申请

US20060105454A1 Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells or the preconditioned cells 审中-公开
标题翻译：分离上皮细胞的方法，预处理细胞的方法以及用上皮细胞或预处理细胞制备生物人造皮肤和真皮的方法
公开(公告)号：US20060105454A1
公开(公告)日：2006-05-18
申请号：US11312113
申请日：2005-12-20
申请人： Young-Sook Son , Hyun-Sook Park , Chun-Ho Kim , Hyun-Ju Kang , Chang-Hwan Kim , Youn-Young Kim , Su-Hyun Lee , Yong-Jae Gin
发明人： Young-Sook Son , Hyun-Sook Park , Chun-Ho Kim , Hyun-Ju Kang , Chang-Hwan Kim , Youn-Young Kim , Su-Hyun Lee , Yong-Jae Gin
IPC分类号： C12N5/06 , C12N5/08
CPC分类号： C12N5/0698 , A61K35/12 , C12N5/0629 , C12N2502/094 , C12N2502/1323 , C12N2502/28 , C12N2509/00 , C12N2509/10
摘要： A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylenediamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be isolated by the method with increased yield, colony forming efficiency (CFE), and colony size. Also, the increased percentage of stem cells in isolated cells is advantageous in therapeutic tissue implantation by autologous or allogeneic transplantation. In skin cells preconditioned by the application of strain, cell division is facilitated, and the secretion of extracellular matrix components and growth factors and the activity of matrix metalloproteinases (MMPs) are improved. When preconditioned cells are implanted by autologous or allogeneic transplantation to heal a damaged tissue, the improved cell adhesion, mobility, and viability provides a biological adjustment effect against a variety of stresses or physical stimuli which the cells would undergo after implantation, with improved capability of integration into host tissue, thereby markedly improving the probability of success in skin grafting.
摘要翻译：一种利用磁力搅拌同时使用胰蛋白酶和乙二胺四乙酸（EDTA）从人皮肤组织或内部器官组织分离上皮细胞的方法，一种通过应用物理刺激预处理分离的生物细胞的方法，即应变，被提供。可以通过增加产量，菌落形成效率（CFE）和菌落大小的方法分离上皮细胞。此外，分离的细胞中干细胞的增加百分比在通过自体或同种异体移植治疗组织植入方面是有利的。在通过应用菌株预处理的皮肤细胞中，促进细胞分裂，细胞外基质成分和生长因子的分泌以及基质金属蛋白酶（MMPs）的活性得到改善。当通过自体或同种异体移植植入预处理细胞以愈合损伤的组织时，改善的细胞粘附性，迁移性和活力提供了针对细胞在植入后将经历的各种应力或物理刺激的生物调节作用，具有改善的整合到宿主组织中，从而显着提高皮肤移植成功的可能性。

3. 发明授权

US06699287B2 Dermal scaffold using alkaline pre-treated chitosan matrix or alkaline pre-treated chitosan and alkaline pre-treated collagen mixed matrix 有权
标题翻译：使用碱性预处理的壳聚糖基质或碱性预处理的壳聚糖和碱性预处理胶原混合基质的真皮支架
公开(公告)号：US06699287B2
公开(公告)日：2004-03-02
申请号：US10132869
申请日：2002-04-25
申请人： Young-Sook Son , Yong-Ha Youn , Seok-Il Hong , Seung-Hoon Lee , Yong-Jae Gin , Kyu-Bo Han , Chun-Ho Kim
发明人： Young-Sook Son , Yong-Ha Youn , Seok-Il Hong , Seung-Hoon Lee , Yong-Jae Gin , Kyu-Bo Han , Chun-Ho Kim
IPC分类号： A61F216
CPC分类号： C12N5/0068 , A61K35/12 , A61L15/225 , A61L15/28 , A61L15/325 , A61L15/425 , A61L27/60 , C12N5/0656 , C12N2533/54 , C12N2533/72 , C08L5/08 , C08L89/06
摘要： Disclosed are a dermal scaffold comprising alkaline pre-treated free amine-containing chitosan matrix, alkaline pre-treated free amine-containing chitosan and alkaline pre-treated collagen mixed matrix, or alkaline pre-treated free amine-containing chitosan and alkaline pre-treated collagen mixed matrix containing chitosan fabrics, which has excellent wound healing effect by constituting microenvironments suitable for migration and proliferation of fibroblasts and vascular cells surrounding the wound to be extremely useful as wound healing dressings, and a bioartificial dermis comprising the dermal scaffold and human fibroblasts, particularly useful for healing broad wound sites such as burns.
摘要翻译：公开了包含碱性预处理的含游离胺的壳聚糖基质，碱性预处理游离胺的脱乙酰壳多糖和碱性预处理胶原混合基质或碱性预处理游离胺的脱乙酰壳多糖和碱性预处理含有壳聚糖织物的胶原混合基质，其通过构成适合于围绕伤口的成纤维细胞和血管细胞的迁移和增殖的微环境具有优异的伤口愈合作用，作为伤口愈合敷料非常有用，以及包含真皮支架和人成纤维细胞的生物人造真皮，特别适用于愈合广泛的伤口部位，如烧伤。

4. 发明申请

US20130085187A1 DEACETYLATION HYDROLASE OF HYALURONIC ACID, HYALURONIC ACID DEACETYLATED BY SAME AND DERIVATIVE THEREOF 有权
标题翻译：羟丙酸脱乙酰壳多糖及其衍生物脱乙酰壳多糖
公开(公告)号：US20130085187A1
公开(公告)日：2013-04-04
申请号：US13703619
申请日：2011-06-10
申请人： Chun-Ho Kim , Jong-Il Kim
发明人： Chun-Ho Kim , Jong-Il Kim
IPC分类号： C12N9/78
CPC分类号： C12Y305/00 , C12N9/80 , C12P19/04
摘要： The present disclosure relates to a deacetylation hydrolase of a hyaluronic acid a hyaluronic acid deacetylated by same and a derivative thereof. The deacetylated hyaluronic acid and the derivative thereof have the following characteristic: a delayed initial decomposition rate on a living body; minimized decrease of molecular weight and viscosity; accelerated gelation due to a lower gelation temperature than the gelation temperature for a non-deacetylated hyaluronic acid; and an hMSC survival rate that is hardly affected by increased concentration of the deacetylated hyaluronic acid and the derivative thereof in a culture medium. As a result, the deacetylated hyaluronic acid and the derivative thereof can be useful as a bioingredient such a delivery system for a cell, gene, drug, and the like, or a support for tissue engineering, etc.
摘要翻译：本公开内容涉及透明质酸的脱乙酰化水解酶，透明质酸由其脱乙酰化的透明质酸及其衍生物。脱乙酰化透明质酸及其衍生物具有以下特征：生物体上延迟的初始分解速率; 最小化分子量和粘度降低; 由于比非脱乙酰化透明质酸的凝胶化温度低的凝胶化温度，加速凝胶化; 和hMSC存活率几乎不受培养基中脱乙酰化透明质酸及其衍生物浓度的增加的影响。结果，脱乙酰化透明质酸及其衍生物可用作细胞，基因，药物等的递送系统或组织工程的支持物等的生物制剂。

5. 发明申请

US20080242850A1 Method of producing chitosan scaffold having high tensile strength and chitosan scaffold produced using the method 有权
标题翻译：使用该方法制备具有高拉伸强度的壳聚糖骨架和壳聚糖支架的方法
公开(公告)号：US20080242850A1
公开(公告)日：2008-10-02
申请号：US12157120
申请日：2008-06-09
申请人： Chun-Ho Kim , Seung-Jae Lee , Jin-Ik Lim , Youngsook Son
发明人： Chun-Ho Kim , Seung-Jae Lee , Jin-Ik Lim , Youngsook Son
IPC分类号： C08B37/08
CPC分类号： C12N5/0068 , A61L15/28 , A61L15/425 , A61L27/20 , A61L27/56 , C12N2533/18 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/70 , C12N2533/72 , C12N2533/74 , C08L5/08
摘要： Provided are a method of producing a porous chitosan scaffold, the method including: providing an aqueous acidic solution having chitosan and a solvent which does not dissolve the chitosan; and freeze-drying the aqueous acidic solution, wherein the solvent is selected from the group consisting of a C3-C8 aliphatic alcohol having one hydroxy group, ethylene glycol monoethylether, ethylene glycol monobutylether, dioxane, tetrahydrofuran, dimethylcarbonate, acetone and acetonitrile, and a chitosan scaffold produced using the method.
摘要翻译：提供一种生产多孔壳聚糖支架的方法，该方法包括：提供具有壳聚糖的水性酸性溶液和不溶解壳聚糖的溶剂; 并冷冻干燥酸性水溶液，其中溶剂选自具有一个羟基的C 3 -C 8脂族醇，乙二醇单乙醚，乙二醇单丁醚，二恶烷，四氢呋喃，碳酸二甲酯，丙酮和乙腈，和使用该方法生产壳聚糖支架。

6. 发明授权

US08697415B2 Deacetylation hydrolase of hyaluronic acid, hyaluronic acid deacetylated by same and derivative thereof 有权
标题翻译：透明质酸的脱乙酰化水解酶，透明质酸与其衍生物脱乙酰化
公开(公告)号：US08697415B2
公开(公告)日：2014-04-15
申请号：US13703619
申请日：2011-06-10
申请人： Chun-Ho Kim , Jong-Il Kim
发明人： Chun-Ho Kim , Jong-Il Kim
IPC分类号： C12N9/78
CPC分类号： C12Y305/00 , C12N9/80 , C12P19/04
摘要： The present disclosure relates to a deacetylation hydrolase of a hyaluronic acid a hyaluronic acid deacetylated by same and a derivative thereof. The deacetylated hyaluronic acid and the derivative thereof have the following characteristic: a delayed initial decomposition rate on a living body; minimized decrease of molecular weight and viscosity; accelerated gelation due to a lower gelation temperature than the gelation temperature for a non-deacetylated hyaluronic acid; and an hMSC survival rate that is hardly affected by increased concentration of the deacetylated hyaluronic acid and the derivative thereof in a culture medium. As a result, the deacetylated hyaluronic acid and the derivative thereof can be useful as a bioingredient such a delivery system for a cell, gene, drug, and the like, or a support for tissue engineering, etc.
摘要翻译：本公开内容涉及透明质酸的脱乙酰化水解酶，透明质酸由其脱乙酰化的透明质酸及其衍生物。脱乙酰化透明质酸及其衍生物具有以下特征：生物体上延迟的初始分解速率; 最小化分子量和粘度降低; 由于比非脱乙酰化透明质酸的凝胶化温度低的凝胶化温度，加速凝胶化; 和hMSC存活率几乎不受培养基中脱乙酰化透明质酸及其衍生物浓度的增加的影响。结果，脱乙酰化透明质酸及其衍生物可用作细胞，基因，药物等的递送系统或组织工程的支持物等的生物制剂。

7. 发明授权

US08691973B2 Method of producing chitosan scaffold having high tensile strength and chitosan scaffold produced using the method 有权
标题翻译：使用该方法制备具有高拉伸强度的壳聚糖骨架和壳聚糖支架的方法
公开(公告)号：US08691973B2
公开(公告)日：2014-04-08
申请号：US12157120
申请日：2007-02-21
申请人： Chun-Ho Kim , Seung-Jae Lee , Jin-Ik Lim , Youngsook Son
发明人： Chun-Ho Kim , Seung-Jae Lee , Jin-Ik Lim , Youngsook Son
IPC分类号： C08B37/08
CPC分类号： C12N5/0068 , A61L15/28 , A61L15/425 , A61L27/20 , A61L27/56 , C12N2533/18 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/70 , C12N2533/72 , C12N2533/74 , C08L5/08
摘要： Provided are a method of producing a porous chitosan scaffold, the method including: providing an aqueous acidic solution having chitosan and a solvent which does not dissolve the chitosan; and freeze-drying the aqueous acidic solution, wherein the solvent is selected from the group consisting of a C3-C8 aliphatic alcohol having one hydroxy group, ethylene glycol monoethylether, ethylene glycol monobutylether, dioxane, tetrahydrofuran, dimethylcarbonate, acetone and acetonitrile, and a chitosan scaffold produced using the method.
摘要翻译：提供一种生产多孔壳聚糖支架的方法，该方法包括：提供具有壳聚糖的水性酸性溶液和不溶解壳聚糖的溶剂; 并冷冻干燥酸性水溶液，其中溶剂选自具有一个羟基的C 3 -C 8脂族醇，乙二醇单乙醚，乙二醇单丁醚，二恶烷，四氢呋喃，碳酸二甲酯，丙酮和乙腈，和使用该方法生产壳聚糖支架。

8. 发明授权

US08189007B2 Graphics engine and method of distributing pixel data 有权
标题翻译：图形引擎和分配像素数据的方法
公开(公告)号：US08189007B2
公开(公告)日：2012-05-29
申请号：US11964961
申请日：2007-12-27
申请人： Chun-Ho Kim
发明人： Chun-Ho Kim
IPC分类号： G09G5/00
CPC分类号： G06T1/20 , G06T11/40
摘要： A graphics engine and related method of operation are disclosed in which a pixel distributor distributes pixel data across a plurality of pixel shaders using a first approach when the presence of one or more rendering features is indicated, else using a second approach different from the first approach.
摘要翻译：公开了一种图形引擎和相关的操作方法，其中当指示存在一个或多个渲染特征时，像素分配器使用第一种方法在多个像素着色器之间分布像素数据，否则使用与第一种方法不同的第二种方法。

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