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1. 发明授权

US5807676A Variety classification method for barley or malt using gene diagnosis and the primer used therefor 失效
标题翻译：使用基因诊断的大麦或麦芽的品种分类方法及其使用的引物
公开(公告)号：US5807676A
公开(公告)日：1998-09-15
申请号：US534684
申请日：1995-09-27
申请人： Youichi Tsuchiya , Shigeki Araki
发明人： Youichi Tsuchiya , Shigeki Araki
IPC分类号： G01N33/566 , C07H21/04 , C12C1/16 , C12N15/09 , C12Q1/68 , C12N15/00 , C12P19/34
CPC分类号： C12Q1/6895
摘要： The variety of barley or malt is quickly and conveniently classified by amplifying the genomic DNA by PCR with the primer consisting of the sequence complementary to the gene that is important for brewing and examining the difference in the base sequence of said DNA.
摘要翻译：通过PCR扩增基因组DNA，通过用与酿造重要的基因互补的序列和检查所述DNA的碱基序列的差异的引物，快速方便地分选大麦或麦芽的种类。

2. 发明授权

US06395500B1 Antibodies for the detection of beer spoilage lactic acid bacteria and kit employing the antibodies 失效
标题翻译：用于检测啤酒腐败乳酸菌的抗体和使用抗体的试剂盒
公开(公告)号：US06395500B1
公开(公告)日：2002-05-28
申请号：US09358258
申请日：1999-07-20
申请人： Youichi Tsuchiya
发明人： Youichi Tsuchiya
IPC分类号： G01N33554
CPC分类号： C07K16/1267 , G01N33/56911 , Y10S435/961 , Y10S435/97 , Y10S435/975 , Y10S436/808 , Y10S436/81
摘要： Antibodies capable of detecting a beer spoilage lactic acid bacterium. Lactic acid bacteria possessing beer spoilage ability are grown in beer, a mammal is immunized to produce antibodies for the detection of beer spoilage lactic acid bacteria, and the beer spoilage lactic acid bacteria are detected using the antibodies. Further, by employing the antibodies, there are provided a method and a kit for detecting a beer spoilage lactic acid bacterium specifically, rapidly, conveniently, and reproducibly, which bacterium contaminates the process of beer production, grows in the produced beer, and lowers the quality of the beer.
摘要翻译：能够检测啤酒腐败乳酸菌的抗体。具有啤酒腐败能力的乳酸菌在啤酒中生长，哺乳动物被免疫以产生用于检测啤酒腐败乳酸菌的抗体，并且使用抗体检测啤酒腐败乳酸菌。此外，通过使用抗体，提供了特异性，快速，方便，可重复地检测啤酒生产啤酒生产过程中生长的啤酒生啤酒中啤酒腐败乳酸菌的方法和试剂盒，并且降低了啤酒质量。

3. 发明授权

US07504239B2 Method of differentiating beer yeast 失效
标题翻译：区分啤酒酵母的方法
公开(公告)号：US07504239B2
公开(公告)日：2009-03-17
申请号：US10067241
申请日：2002-02-07
申请人： Masahide Sato , Youichi Tsuchiya
发明人： Masahide Sato , Youichi Tsuchiya
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6895 , C12Q2600/158
摘要： The method of differentiating beer yeast of the invention is a method which comprises a first step of synthesizing a primer capable of amplifying the linker portion between a base sequence (A) and a base sequence (B) in a novel gene (C) which has the base sequence (B) comprising a portion of yeast chromosome IX linked downstream from the base sequence (A) comprising a portion of the N-terminal end of yeast gene Lg-FLO1, and which includes the base sequences listed as SEQ. ID. Nos. 1-6 of the Sequence Listing; a second step of carrying out a PCR (Polymerase Chain Reaction) using the primer synthesized in the first step and DNA separated from a yeast specimen; and a third step of differentiating whether the yeast is bottom-fermenting yeast or wild yeast, based on the PCR amplification product obtained from the second step.
摘要翻译：本发明的区别啤酒酵母的方法是一种方法，该方法包括：在新基因（C）中合成能够扩增碱基序列（A）和碱基序列（B）之间的接头部分的引物的第一步骤，其具有所述碱基序列（B）包含与包含酵母基因Lg-FLO1的N-末端的一部分的碱基序列（A）下游连接的酵母染色体IX的一部分，并且其包含SEQ ID NO：1所示的碱基序列。 ID。序列表第1-6项; 使用第一步合成的引物和从酵母标本分离的DNA进行PCR（聚合酶链反应）的第二步骤; 以及基于从第二步获得的PCR扩增产物来区分酵母是否是底部发酵酵母或野生酵母的第三步骤。

4. 发明授权

US6108296A Optical recording disc capable of preventing illegal copy 有权
公开(公告)号：US6108296A
公开(公告)日：2000-08-22
申请号：US136458
申请日：1998-08-19
申请人： Seiji Kajiyama , Youichi Tsuchiya , Kenji Torazawa , Kouichi Tada
发明人： Seiji Kajiyama , Youichi Tsuchiya , Kenji Torazawa , Kouichi Tada
IPC分类号： G11B7/013 , G11B7/24 , G11B7/26 , G11B20/00 , G11B23/28
CPC分类号： G11B23/281 , G11B23/284 , G11B7/24 , G11B7/24085 , G11B20/00086 , G11B7/261
摘要： Micro-pits or micro-pit strings, which are disabled precise copying by means of illegal copying described above and enable signals different from signals of a normal disc to be detected in case of failure in precise copying, are recorded in a copy protected area of an optical disc beforehand. A read unit checks the copy protected area and determines a loaded disc is legal disc or illegally copied disc. If illegal, reading is prohibitted.

5. 发明申请

US20090142750A1 Method of detecting and identifying gram-negative obligative anaerobic bacterium 审中-公开
标题翻译：检测和鉴定革兰氏阴性义务厌氧菌的方法
公开(公告)号：US20090142750A1
公开(公告)日：2009-06-04
申请号：US10589493
申请日：2005-02-16
申请人： Yasukazu Nakakita , Youichi Tsuchiya
发明人： Yasukazu Nakakita , Youichi Tsuchiya
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/689
摘要： There is provided a method of detecting potentially beer-spoiling obligatory anaerobic gram-negative bacteria, and a method of simultaneously detecting and distinguishing different beer-spoilage microorganisms including such bacteria.
摘要翻译：提供了一种检测潜在的啤酒破坏强制性厌氧革兰氏阴性细菌的方法，以及同时检测和区分包括这种细菌的不同啤酒腐败微生物的方法。

6. 发明申请

US20070292852A1 Method of Detecting and Identifying Lactic Acid Bacterium 审中-公开
标题翻译：检测和鉴定乳酸杆菌的方法
公开(公告)号：US20070292852A1
公开(公告)日：2007-12-20
申请号：US10589389
申请日：2005-02-16
申请人： Yasukazu Nakakita , Youichi Tsuchiya
发明人： Yasukazu Nakakita , Youichi Tsuchiya
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/31
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： There are provided a method of detecting potentially beer-spoiling lactic acid bacteria, and a method of simultaneously detecting and distinguishing different lactic acid bacteria including such bacteria.
摘要翻译：提供了一种检测潜在的啤酒破乳乳酸菌的方法，以及同时检测和区分包括这种细菌的不同乳酸菌的方法。

7. 发明申请

US20070207458A1 PRIMER FOR DETECTING ALICYCLOBACILLUS 审中-公开
标题翻译：用于检测ALICYCLOBACILLUS的PRIMER
公开(公告)号：US20070207458A1
公开(公告)日：2007-09-06
申请号：US10575102
申请日：2004-10-08
申请人： Yasukazu Nakakita , Masahiro Ogawa , Youichi Tsuchiya
发明人： Yasukazu Nakakita , Masahiro Ogawa , Youichi Tsuchiya
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q1/6844 , C12Q2600/16
摘要： Primers specific for an Alicyclobacillus spp. are used to amplify specific regions of 16S rDNA gene of the Alicyclobacillus spp., and the presence or absence of the amplified product is confirmed to detect the Alicyclobacillus spp. (especially Alicyclobacillus acidoterrestris).
摘要翻译：对脂环芽孢杆菌属特异性的引物用于扩增脂环酸杆菌属的16S rDNA基因的特异性区域，确认扩增产物的存在或不存在以检测脂环酸杆菌属。（特别是酸性脂环酸杆菌）。

8. 发明授权

US06438097B1 Optical recording disc capable of preventing illegal copy 失效
标题翻译：能够防止非法复制的光记录盘
公开(公告)号：US06438097B1
公开(公告)日：2002-08-20
申请号：US09487867
申请日：2000-01-20
申请人： Seiji Kajiyama , Youichi Tsuchiya , Kenji Torazawa , Kouichi Tada
发明人： Seiji Kajiyama , Youichi Tsuchiya , Kenji Torazawa , Kouichi Tada
IPC分类号： G11B724
CPC分类号： G11B23/281 , G11B7/24 , G11B7/24085 , G11B7/261 , G11B20/00086 , G11B23/284
摘要： Micro-pits or micro-pit strings, which are disabled precise copying by means of illegal copying described above and enable signals different from signals of a normal disc to be detected in case of failure in precise copying, are recorded in a copy protected area of an optical disc beforehand. A read unit checks the copy protected area and determines a loaded disc is legal disc or illegally copied disc. If illegal, reading is prohibitted.
摘要翻译：通过上述非法复制而禁止精确复制的微凹坑或微凹坑串被记录在复制保护区域的复制保护区域中，并且使得在精确复制失败的情况下能够检测到与普通盘的信号不同的信号被检测到光盘。读取单元检查复制保护区域，并确定加载的光盘是合法光盘或非法复制光盘。如果非法，阅读被禁止。

9. 发明授权

US06262967B1 Optical recording disc capable of preventing illegal copy 有权
标题翻译：能够防止非法复制的光记录盘
公开(公告)号：US06262967B1
公开(公告)日：2001-07-17
申请号：US09487866
申请日：2000-01-20
申请人： Seiji Kajiyama , Youichi Tsuchiya , Kenji Torazawa , Kouichi Tada
发明人： Seiji Kajiyama , Youichi Tsuchiya , Kenji Torazawa , Kouichi Tada
IPC分类号： G11B724
CPC分类号： G11B23/281 , G11B7/24 , G11B7/24085 , G11B7/261 , G11B20/00086 , G11B23/284
摘要： An illegal disc copying protection system which includes an optical disc with recorded information as a series of pits aligned to a track direction from the optical disc, a recording system which records information as a series of pits aligned to the track direction from the optical disc, and a reading system which reads information being recorded as a series of pits aligned to the track direction from the optical disc.
摘要翻译：一种非法盘片复制保护系统，其包括具有记录信息的光盘作为与光盘的轨道方向对齐的一系列凹坑;记录系统，其将信息作为与光盘方向对准的一系列凹坑记录，以及读取系统，其读取被记录为与光盘对准的轨道方向的一系列凹坑的信息。

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