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1. 发明申请

US20110236900A1 NOVEL MUTS PROTEIN AND METHOD FOR DETERMING MUTATION USING THE SAME 审中-公开
标题翻译：新颖的蛋白质和使用其确定突变的方法
公开(公告)号：US20110236900A1
公开(公告)日：2011-09-29
申请号：US13130993
申请日：2009-11-27
申请人： Yoshihide Hayashizaki , Masayoshi Itoh , Hajime Kanamori , Kengo Usui , Kazuhito Nomura
发明人： Yoshihide Hayashizaki , Masayoshi Itoh , Hajime Kanamori , Kengo Usui , Kazuhito Nomura
IPC分类号： C12Q1/68 , C07K14/195 , C07H21/04 , C12N5/10 , C12P21/06 , C12N1/00
CPC分类号： C07K14/195 , G01N33/5308 , G01N2333/32
摘要： A method for determining the presence or absence of a mutation on the basis of the presence or absence of amplification with high reliability is provided. A target sequence including a target site contained in a sample nucleic acid is amplified using a primer that can hybridize to a region including the target site contained in the sample nucleic acid in the presence of a novel MutS having an amino acid sequence of SEQ ID NO: 2, and then the presence or absence of a mutation at the target site is determined on the basis of the presence or absence of amplification. The novel MutS binds more specifically to a mismatched base pair than to a fully-matched base pair, whereby an extension reaction caused by a mismatch-binding primer is suppressed. Thus, according to the present invention, the presence or absence of a mutation can be determined with high reliability.
摘要翻译：提供了一种基于高可靠性的扩增的存在或不存在来确定突变的存在或不存在的方法。包含样品核酸中包含的靶位点的靶序列使用可以在含有氨基酸序列SEQ ID NO的新型MutS的存在下与包含在样品核酸中的靶位点的区域杂交的引物进行扩增：2，然后基于扩增的存在或不存在来确定靶位点处的突变的存在或不存在。新的MutS更具体地结合失配的碱基对而不是完全匹配的碱基对，由此抑制由错配结合引物引起的延伸反应。因此，根据本发明，可以高可靠性确定突变的存在或不存在。

2. 发明申请

US20100221787A1 ISOTHERMAL AMPLIFICATION METHOD AND DNA POLYMERASE USED IN THE SAME 审中-公开
标题翻译：同位素放大方法和使用其中的DNA聚合酶
公开(公告)号：US20100221787A1
公开(公告)日：2010-09-02
申请号：US12739496
申请日：2008-10-24
申请人： Yoshihide Hayashizaki , Masayoshi Itoh , Alexander Lezhava , Yoshimi Benno , Yasumasa Mitani , Hajime Kanamori
发明人： Yoshihide Hayashizaki , Masayoshi Itoh , Alexander Lezhava , Yoshimi Benno , Yasumasa Mitani , Hajime Kanamori
IPC分类号： C12P19/34 , C12N9/12
CPC分类号： C12Q1/6846 , C12N9/1252 , C12P19/34 , C12Q2525/155 , C12Q2521/107 , C12Q2527/101 , C12Q2531/107
摘要： A DNA polymerase suitable for specific isothermal amplification methods and an isothermal amplification method using the DNA polymerase are provided. In the presence of a DNA polymerase including a protein described in the following item (a) or (b), an amplification reaction of a target nucleic acid sequence in a nucleic acid sample is carried out isothermally using a first primer shown in the following (X). By using the DNA polymerase, it becomes possible to carry out the amplification reaction using the primer within a shorter time than ever before. (a) a protein having an amino acid sequence represented by SEQ ID NO. 23 (b) a protein having an amino acid sequence represented by SEQ ID NO. 25 (X) a primer that contains, in a 3′ end portion, a sequence (Ac′) that hybridizes to a sequence (A) of a 3′ end portion of the target nucleic acid sequence and also contains, on a 5′ side of the sequence (Ac′), a sequence (B′) that hybridizes to a complementary sequence (Bc) to a sequence (B) present on a 5′ side with respect to the sequence (A) in the target nucleic acid sequence
摘要翻译：提供了适用于特定等温扩增方法的DNA聚合酶和使用DNA聚合酶的等温扩增方法。在包含下述（a）或（b）中所述的蛋白质的DNA聚合酶的存在下，使用下述第一引物等温进行靶核酸序列的扩增反应 X）。通过使用DNA聚合酶，可以在比以往更短的时间内使用引物进行扩增反应。（a）具有SEQ ID NO：1所示的氨基酸序列的蛋白质。 23（b）具有SEQ ID NO：1所示的氨基酸序列的蛋白质。 25（X）引物，其在3'末端含有与目标核酸序列的3'端部分的序列（A）杂交的序列（Ac'），并且在5' 序列（Ac'）侧，与靶序列（A）相对于存在于5'侧的序列（B）的互补序列（Bc）杂交的序列（B'）

3. 发明授权

US07673864B2 Folding device and printing system 失效
标题翻译：折叠装置和打印系统
公开(公告)号：US07673864B2
公开(公告)日：2010-03-09
申请号：US11596888
申请日：2005-06-20
申请人： Yasuhisa Mizuno , Hajime Kanamori , Riki Murakami
发明人： Yasuhisa Mizuno , Hajime Kanamori , Riki Murakami
IPC分类号： B41L1/32
CPC分类号： B65H45/1015 , B65H23/042 , B65H45/107 , B65H45/109
摘要： A folding device for folding continuous paper, which is previously provided with fold lines at given intervals, along the fold lines in a zigzag pattern. The folding device comprises a feeding member of the continuous paper, a swinger fin that swings using its one end as a fulcrum like a pendulum in synchronization with the fold lines while feeding the continuous paper into its inside, and a table to be surmounted by the continuous paper that has been folded. The swinger fin includes a feed roller pair for feeding the continuous paper while sandwiching the paper therebetween, and at least a pair of guide plates facing each other with a given gap therebetween, in which the continuous paper is fed through the gap. One of the feed rollers constructing the feed roller pair and one of the guide plates, which are located on one side of the continuous paper, can be integrally opened or detached from the swinger fin.
摘要翻译：用于折叠连续纸的折叠装置，其以预定的间隔以给定的间隔以折叠线以锯齿形图案设置。折叠装置包括连续纸的进给构件，摆动翅片，其在将连续纸送入其内部的同时使用其一端作为支点与倾斜同步的摆锤作为支点摆动，并且将由桌子已经折叠的连续纸。摆动翼片包括用于在将纸张夹在其间的同时馈送连续纸的进给辊对以及至少一对彼此面对的引导板，其中连续的纸被供给通过间隙。构成进给辊对的进给辊之一和位于连续纸的一侧上的导向板中的一个可以与摆动翅片一体地打开或分离。

4. 发明申请

US20080070772A1 Folding Device and Printing System 失效
标题翻译：折叠装置和打印系统
公开(公告)号：US20080070772A1
公开(公告)日：2008-03-20
申请号：US11596888
申请日：2005-06-20
申请人： Yasuhisa Mizuno , Hajime Kanamori , Riki Murakami , Munekazu Ikeda , Hidetoshi Hara
发明人： Yasuhisa Mizuno , Hajime Kanamori , Riki Murakami , Munekazu Ikeda , Hidetoshi Hara
IPC分类号： B31F1/10
CPC分类号： B65H45/1015 , B65H23/042 , B65H45/107 , B65H45/109
摘要： A folding device for folding continuous paper, which is previously provided with fold lines at given intervals, along the fold lines in a zigzag pattern. The folding device comprises a feeding member of the continuous paper, a swinger fin that swings using its one end as a fulcrum like a pendulum in synchronization with the fold lines while feeding the continuous paper into its inside, and a table to be surmounted by the continuous paper that has been folded. The swinger fin includes a feed roller pair for feeding the continuous paper while sandwiching the paper therebetween, and at least a pair of guide plates facing each other with a given gap therebetween, in which the continuous paper is fed through the gap. One of the feed rollers constructing the feed roller pair and one of the guide plates, which are located on one side of the continuous paper, can be integrally opened or detached from the swinger fin.
摘要翻译：用于折叠连续纸的折叠装置，其以预定的间隔以给定的间隔以折叠线以锯齿形图案设置。折叠装置包括连续纸的进给构件，摆动翅片，其在将连续纸送入其内部的同时使用其一端作为支点与倾斜同步的摆锤作为支点摆动，并且将由桌子已经折叠的连续纸。摆动翼片包括用于在将纸张夹在其间的同时馈送连续纸的进给辊对以及至少一对彼此面对的引导板，其中连续的纸被供给通过间隙。构成进给辊对的进给辊之一和位于连续纸的一侧上的导向板中的一个可以与摆动翅片一体地打开或分离。

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