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1. 发明授权

US08415116B2 Method for pre-treating specimen and method for analyzing specimen 失效
公开(公告)号：US08415116B2
公开(公告)日：2013-04-09
申请号：US12113795
申请日：2008-05-01
申请人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu , Kazuhiro Ban
发明人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu , Kazuhiro Ban
IPC分类号： C12Q1/02 , G01N1/30 , G01N1/00
CPC分类号： G01N1/30 , G01N1/31 , G01N2001/282 , G01N2001/317
摘要： A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method.

2. 发明申请

US20080179512A1 METHOD FOR OBTAINING INFORMATION AND DEVICE THEREFOR 有权
标题翻译：获取信息的方法及其设备
公开(公告)号：US20080179512A1
公开(公告)日：2008-07-31
申请号：US12017584
申请日：2008-01-22
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
IPC分类号： B01D59/44
CPC分类号： H01J49/142
摘要： A method for obtaining information on a mass of an object by time-of-flight mass spectrometry. This method includes placing colloidal metal particles for promoting ionization of the object inside the object at a depth ranging from 0.1 nm to 100 nm in opposition to a primary beam for the ionization; irradiating the object with the primary beam selected from the group of ions, neutral particles, and electrons, which can be focused, pulsed, and are capable of scanning, and laser beams, which can be focused, pulsed, and are capable of scanning to ionize a constituent of the object and to allow the ionized constituent to fly out of the object; and obtaining information on the mass of the flying constituent of the object by time-of-flight mass spectrometry.
摘要翻译：一种通过飞行时间质谱法获得物体质量信息的方法。该方法包括将胶体金属颗粒放置在与离子化的主光束相反的0.1nm至100nm范围内的物体内部物体的离子化; 用可以被聚焦，脉冲和能够扫描的离子，中性粒子和电子组中选择的主要束照射物体，以及可以被聚焦，脉冲且能够扫描的激光束电离物体的成分并使离子化成分从物体飞出; 并通过飞行时间质谱法获得关于物体飞行成分质量的信息。

3. 发明授权

US07956321B2 Method of measuring target object in a sample using mass spectrometry 有权
标题翻译：使用质谱法测定样品中的目标物体的方法
公开(公告)号：US07956321B2
公开(公告)日：2011-06-07
申请号：US12239344
申请日：2008-09-26
申请人： Yohei Murayama , Manabu Komatsu , Hiroyuki Hashimoto
发明人： Yohei Murayama , Manabu Komatsu , Hiroyuki Hashimoto
IPC分类号： H01J49/00
CPC分类号： H01J49/40 , H01J49/0004 , Y10T436/24
摘要： In-plane distribution of a target object contained in a sample is measured. The sample dispersedly placed on a substrate is treated to promote ionization of the target object. Then, the mass and flying amount of an ion containing the target object or a component thereof is determined by irradiating an ion beam to the sample and performing time-of-flight secondary ion mass spectrometry of the ion that flies from a portion in the sample where the ion beam is irradiated, and the in-plane distribution of the target object is determined from the mass and flying amount data obtained at plural portions by scanning the beam on the sample plane. The step of treating the sample to promote ionization of the target object includes contacting an aqueous solution of an acid that does not crystallize at ordinary temperature with the sample. A high spatial resolution two-dimensional image can be obtained.
摘要翻译：测量包含在样本中的目标对象的平面内分布。处理分散放置在基板上的样品，以促进目标物体的电离。然后，通过向样品照射离子束并进行从样品的一部分飞出的离子的飞行时间二次离子质谱法来确定含有目标物体或其成分的离子的质量和飞散量其中照射离子束，并且通过在样品平面上扫描光束从多个部分获得的质量和飞行量数据确定目标物体的面内分布。处理样品以促进目标物体的电离的步骤包括使常温下不结晶的酸的水溶液与样品接触。可以获得高空间分辨率的二维图像。

4. 发明授权

US07560689B2 High-sensitivity mass spectrometer and method 失效
标题翻译：高灵敏度质谱仪及方法
公开(公告)号：US07560689B2
公开(公告)日：2009-07-14
申请号：US11682500
申请日：2007-03-06
申请人： Kazuhiro Ban , Hiroyuki Hashimoto , Manabu Komatsu , Norihiko Utsunomiya , Yohei Murayama
发明人： Kazuhiro Ban , Hiroyuki Hashimoto , Manabu Komatsu , Norihiko Utsunomiya , Yohei Murayama
IPC分类号： B01D59/44 , H01J49/00 , H01J49/40 , G01N31/00 , G01N33/00 , G01N23/00 , G21K7/00
CPC分类号： H01J49/0418
摘要： A sample is sliced obliquely with a blade provided with a metal thin film on the surface, and the slice of the sample adhering to the blade surface is subjected to mass spectrometry. As a result, the section of the sliced sample can be analyzed immediately by mass spectrometry with high sensitivity.
摘要翻译：将样品用表面上设置有金属薄膜的刀片倾斜切片，并且将附着在刀片表面上的样品切片进行质谱分析。因此，可以通过高灵敏度的质谱立即分析切片样品的部分。

5. 发明授权

US07446309B2 In-plane distribution measurement method 有权
标题翻译：平面内分布测量方法
公开(公告)号：US07446309B2
公开(公告)日：2008-11-04
申请号：US11283912
申请日：2005-11-22
申请人： Yohei Murayama , Manabu Komatsu , Hiroyuki Hashimoto
发明人： Yohei Murayama , Manabu Komatsu , Hiroyuki Hashimoto
IPC分类号： H01J49/00
CPC分类号： H01J49/40 , H01J49/0004 , Y10T436/24
摘要： In plane distribution of a target object contained in a sample is measured. The sample dispersedly placed on a substrate is treated to promote ionization of the target object. Then, the mass and flying amount of an ion containing the target object or a component thereof is determined by irradiating an ion beam to the sample and performing time-of-flight secondary ion mass spectrometry of the ion that flies from a portion in the sample where the ion beam is irradiated, and the in-plane distribution of the target object is determined from the mass and flying amount data obtained at plural portions by scanning the beam on the sample plane. The step of treating the sample to promote ionization of the target object includes contacting an aqueous solution of an acid that does not crystallize at ordinary temperature with the sample. A high spatial resolution two-dimensional image can be obtained.
摘要翻译：测量样品中包含的目标物体的平面分布。处理分散放置在基板上的样品，以促进目标物体的电离。然后，通过向样品照射离子束并进行从样品的一部分飞出的离子的飞行时间二次离子质谱法来确定含有目标物体或其成分的离子的质量和飞散量其中照射离子束，并且通过在样品平面上扫描光束从多个部分获得的质量和飞行量数据确定目标物体的面内分布。处理样品以促进目标物体的电离的步骤包括使常温下不结晶的酸的水溶液与样品接触。可以获得高空间分辨率的二维图像。

6. 发明授权

US09086404B2 Method for treating living samples and analyzing the same 有权
标题翻译：处理活体样本并进行分析的方法
公开(公告)号：US09086404B2
公开(公告)日：2015-07-21
申请号：US11867276
申请日：2007-10-04
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Kazuhiro Ban , Yohei Murayama
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Kazuhiro Ban , Yohei Murayama
IPC分类号： G01N33/50 , G01N35/10
CPC分类号： G01N33/5082 , G01N35/1002 , G01N2035/1041
摘要： A method is provided by which a reagent is provided to a living sample, including contacting a thin-film containing a porous body with a living sample containing cells or tissues, and discharging a solution containing at least one or more reagents to a non-contact side of the thin-film by ink-jet to provide the solution to the specific area of the living sample through pores on the thin-film, wherein the smaller diameter of the pores on the porous body relative to the diameter of cells in the living sample enables the reagent to be provided to each individual cell separately. According to this method, the reagent and the reaction product can be fixed while maintaining positioning information of the target substance in the living sample, as a pretreatment for analyzing with high positioning accuracy the target substance in each individual cell in the living sample.
摘要翻译：提供了一种将试剂提供给活体样品的方法，包括使含有多孔体的薄膜与含有细胞或组织的活体样品接触，并将含有至少一种或多种试剂的溶液排出至非接触通过喷墨使薄膜的一侧通过薄膜上的孔提供到活体样品的特定区域的溶液，其中多孔体上的孔的直径相对于活体中细胞的直径较小样品使得可以分别向每个单独的细胞提供试剂。根据该方法，可以在维持活体样品中的目标物质的定位信息的同时固定试剂和反应产物，作为用于以高定位精度分析活体样品中各个细胞中的目标物质的预处理。

7. 发明申请

US20140087408A1 INFORMATION ACQUISITION METHOD 审中-公开
标题翻译：信息获取方法
公开(公告)号：US20140087408A1
公开(公告)日：2014-03-27
申请号：US13995645
申请日：2011-11-28
申请人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu
发明人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu
IPC分类号： G01N33/574
CPC分类号： G01N33/57415 , C07K14/4756 , G01N33/6848 , G01N2333/4756
摘要： The present invention relates to an information acquisition method of acquiring information of distribution of a protein or peptide in a sample based on mass information obtained by mass spectrometry of the protein or peptide. The method includes mass spectrometry of a definite region of the sample after limited proteolysis of the protein or peptide and acquisition of information relating to distribution using an ion peak that has a two-dimensional intensity distribution having a Pearson product-moment correlation coefficient of 0.5 to more and 1.0 or less in the definite region against the two-dimensional intensity distribution of the parent ion of the protein or the peptide subjected to the limited proteolysis and has a peak intensity ratio of larger than 1.0 against the peak intensity of the integrated spectrum of the parent ion in the definite region, wherein the m/z of the ion peak is greater than 500.
摘要翻译：本发明涉及基于通过蛋白质或肽的质谱法获得的质量信息获取样品中蛋白质或肽的分布信息的信息获取方法。该方法包括在蛋白质或肽的有限蛋白水解之后样品的确定区域的质谱，并且使用具有Pearson积矩相关系数为0.5的二维强度分布的离子峰获取与分布有关的信息相对于蛋白质或经受有限蛋白水解的肽的母离子的二维强度分布，确定区域中的1.0和更小，并且具有大于1.0的峰强度比与峰积分的峰强度比该定子区域中的母离子，其中离子峰的m / z大于500。

8. 发明申请

US20090266982A1 MASS SPECTROMETRY SUBSTRATE AND MASS SPECTROMETRY METHOD 有权
标题翻译：质谱法基质和质谱法
公开(公告)号：US20090266982A1
公开(公告)日：2009-10-29
申请号：US12427583
申请日：2009-04-21
申请人： Kimihiro Yoshimura , Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama
发明人： Kimihiro Yoshimura , Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama
IPC分类号： B01D59/44 , H01J49/00
CPC分类号： H01J49/0418 , Y10T436/24 , Y10T436/25 , Y10T436/25875
摘要： A mass spectrometry method that makes it possible to perform high-sensitivity detection of a desorbed/ionized object substance to be measured in mass spectrometry in which the object substance to be measured is desorbed and ionized. The method includes placing at least an ionizing agent having two or more functional groups represented by Formula (1) below in a molecule: —(CF2)COOH (1) wherein the ionizing agent has a boiling point of equal to or higher than 150° C. and an object molecule to be measured on a substrate and irradiating the ionizing agent and the object molecule to be measured with a primary beam selected from ions, neutral particles, electrons, and a laser beam.
摘要翻译：能够对被测定物被解吸和离子化的质谱法进行能够进行待测量的解吸附电离对象物质的高灵敏度检测的质谱法。该方法包括在分子中至少放置一个具有两个或多个由式（1）表示的官能团的电离剂： - （CF 2）COOH（1）其中电离剂的沸点等于或高于150℃ 和待测量的物体分子，并用从离子，中性粒子，电子和激光束中选择的一次束照射被测量的电离剂和待测物体。

9. 发明授权

US07851749B2 Method for obtaining information and device therefor 有权
标题翻译：获取信息的方法及其设备
公开(公告)号：US07851749B2
公开(公告)日：2010-12-14
申请号：US12017584
申请日：2008-01-22
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
IPC分类号： H01J49/00
CPC分类号： H01J49/142
摘要： A method for obtaining information on a mass of an object by time-of-flight mass spectrometry. This method includes placing colloidal metal particles for promoting ionization of the object inside the object at a depth ranging from 0.1 nm to 100 nm in opposition to a primary beam for the ionization; irradiating the object with the primary beam selected from the group of ions, neutral particles, and electrons, which can be focused, pulsed, and are capable of scanning, and laser beams, which can be focused, pulsed, and are capable of scanning to ionize a constituent of the object and to allow the ionized constituent to fly out of the object; and obtaining information on the mass of the flying constituent of the object by time-of-flight mass spectrometry.
摘要翻译：一种通过飞行时间质谱法获得物体质量信息的方法。该方法包括将胶体金属颗粒放置在与离子化的主光束相反的0.1nm至100nm范围内的物体内部物体的离子化; 用可以被聚焦，脉冲和能够扫描的离子，中性粒子和电子组中选择的主要束照射物体，以及可以被聚焦，脉冲且能够扫描的激光束电离物体的成分并使离子化成分从物体飞出; 并通过飞行时间质谱法获得关于物体飞行成分质量的信息。

10. 发明授权

US07795579B2 Information obtaining method 有权
标题翻译：信息获取方法
公开(公告)号：US07795579B2
公开(公告)日：2010-09-14
申请号：US12120512
申请日：2008-05-14
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
IPC分类号： B01D59/44
CPC分类号： H01J49/145
摘要： An information obtaining method for obtaining information about a mass of a component of an analyte using a time of flight mass spectrometer and obtaining information about a distribution state of the component based on the obtained information about the mass includes the step of: (1) adding a self-reactive substance to the analyte on a base to facilitate ionization of the component; (2) irradiating the analyte with a primary beam in the presence of the self-reactive substance, thereby ionizing the components and allowing resulting ions to fly; (3) obtaining information about mass of the flying ions using the time of flight mass spectrometer; and (4) obtaining information about the distribution state of the component on the base based on the information about the mass.
摘要翻译：使用飞行时间质谱仪获取关于分析物的成分的质量的信息的信息获取方法，基于获得的关于质量的信息获得关于部件的分布状态的信息，包括以下步骤：（1）添加对基底上的分析物的自反应物质，以促进组分的离子化; （2）在存在自反应物质的情况下用主光束照射分析物，从而电离成分并允许所得离子飞行; （3）使用飞行时间质谱仪获取关于飞行离子质量的信息; 和（4）基于关于质量的信息获得关于组件在基础上的分布状态的信息。

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