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1. 发明申请

US20060053502A1 Systems and methods for protein production 有权
公开(公告)号：US20060053502A1
公开(公告)日：2006-03-09
申请号：US11217137
申请日：2005-09-01
申请人： Yijie Gao , Nicole Piche , Mei Geng , Stephen Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
发明人： Yijie Gao , Nicole Piche , Mei Geng , Stephen Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
IPC分类号： A01K67/027 , C07H21/04 , C12P21/06 , C12N9/64 , C12N5/06 , C12N5/04
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02
摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6-mediated UPR pathway.

2. 发明申请

US20120322104A1 SYSTEMS AND METHODS FOR PROTEIN PRODUCTION 审中-公开
标题翻译：蛋白质生产的系统和方法
公开(公告)号：US20120322104A1
公开(公告)日：2012-12-20
申请号：US13446649
申请日：2012-04-13
申请人： Yijie GAO , Nicloe M. Piche , Mei Geng , Stephen H. Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
发明人： Yijie GAO , Nicloe M. Piche , Mei Geng , Stephen H. Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
IPC分类号： C12N5/10 , C12P21/00 , C12N15/79 , C12N5/16
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02
摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6-mediated UPR pathway.
摘要翻译：本发明涉及生产目标蛋白质的系统和方法。本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。一方面，本发明的特征在于遗传工程改造的细胞，其包含编码（1）目的蛋白质的一种或多种重组表达盒，和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XBP1或ATF6介导的UPR途径的组分或调节剂。

3. 发明授权

US08173394B2 Systems and methods for protein production 有权
标题翻译：蛋白质生产的系统和方法
公开(公告)号：US08173394B2
公开(公告)日：2012-05-08
申请号：US12419018
申请日：2009-04-06
申请人： Yijie Gao , Nicole M. Piche , Mei Geng , Stephen H. Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
发明人： Yijie Gao , Nicole M. Piche , Mei Geng , Stephen H. Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
IPC分类号： C12P21/06 , C12N5/00
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02
摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XB1- or ATF6-mediated UPR pathway.
摘要翻译：本发明涉及生产目标蛋白质的系统和方法。本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。一方面，本发明的特征在于遗传工程改造的细胞，其包含编码（1）目的蛋白质的一种或多种重组表达盒，和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XB1或ATF6介导的UPR途径的组分或调节剂。

4. 发明申请

US20090221035A1 SYSTEMS AND METHODS FOR PROTEIN PRODUCTION 有权
标题翻译：蛋白质生产的系统和方法
公开(公告)号：US20090221035A1
公开(公告)日：2009-09-03
申请号：US12419018
申请日：2009-04-06
申请人： Yijie Gao , Nicole M. Piche , Mei Geng , Stephen H. Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
发明人： Yijie Gao , Nicole M. Piche , Mei Geng , Stephen H. Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
IPC分类号： C12P21/00 , C12N5/04 , C12N5/06 , A01K67/027 , C12N15/00
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02
摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XB1- or ATF6-mediated UPR pathway.
摘要翻译：本发明涉及生产目标蛋白质的系统和方法。本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。一方面，本发明的特征在于遗传工程改造的细胞，其包含一个或多个编码（1）目的蛋白质的重组表达盒，和（2）在细胞的未折叠的蛋白质应答（UPR）途径中有功能的多肽。多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XB1或ATF6介导的UPR途径的组分或调节剂。

5. 发明授权

US07514237B2 Systems and methods for protein production 有权
标题翻译：蛋白质生产的系统和方法
公开(公告)号：US07514237B2
公开(公告)日：2009-04-07
申请号：US11217137
申请日：2005-09-01
申请人： Yijie Gao , Nicole M. Piche , Mei Geng , Stephen H. Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
发明人： Yijie Gao , Nicole M. Piche , Mei Geng , Stephen H. Herrmann , Xiaotian Zhong , Ronald Kriz , Zhijian Lu
IPC分类号： C12P21/06 , C12N1/20
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02
摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6-mediated UPR pathway.
摘要翻译：本发明涉及生产目标蛋白质的系统和方法。本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。一方面，本发明的特征在于遗传工程改造的细胞，其包含编码（1）目的蛋白质的一种或多种重组表达盒，和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XBP1或ATF6介导的UPR途径的组分或调节剂。

6. 发明申请

US20070161047A1 Methods of labeling transiently expressed proteins in large-scale eukaryotic cell cultures 审中-公开
标题翻译：在大规模真核细胞培养物中标记瞬时表达的蛋白质的方法
公开(公告)号：US20070161047A1
公开(公告)日：2007-07-12
申请号：US11651135
申请日：2007-01-09
申请人： Xiaotian Zhong , Ronald Kriz
发明人： Xiaotian Zhong , Ronald Kriz
IPC分类号： G01N33/53 , C12P21/06 , C12N1/18 , C12N5/06 , C07K14/47
CPC分类号： C07K1/13 , C12N9/12 , C12P21/02
摘要： The present invention is based on observations that transiently transfecting large-scale eukaryotic cell cultures with a polynucleotide encoding a protein of interest can be used to rapidly produce the large quantities of labeled proteins required for various biochemical techniques such as spectroscopy, microscopy, and crystallography, and applications including protein structure determination, protein tracing and/or localization, diagnostic and therapeutic applications, and affinity experiments. Thus, the present invention provides methods for rapidly producing large quantities of labeled proteins by using transient transfection of large-scale eukaryotic cell cultures, which are then grown in a chemically defined labeling medium that includes labeled amino acids. The present invention is also directed to methods of using the labeled proteins produced by the novel labeling methods for use in various techniques.
摘要翻译：本发明基于以编码目的蛋白质的多核苷酸瞬时转染大规模真核细胞培养物的观察结果，可以用于快速产生各种生物化学技术如光谱学，显微镜和晶体学所需的大量标记蛋白质，以及包括蛋白质结构测定，蛋白质追踪和/或定位，诊断和治疗应用以及亲和力实验的应用。因此，本发明提供了通过使用大规模真核细胞培养物的瞬时转染来快速产生大量标记的蛋白质的方法，然后将其在包括标记的氨基酸的化学学定义的标记培养基中生长。本发明还涉及使用通过新型标记方法产生的标记蛋白用于各种技术的方法。

7. 发明申请

US20080280313A1 Coccidian Parasite Casein Kinase I as a Chemotherapeutic Target for Antiprotozoal Agents 失效
标题翻译：球虫寄生虫酪蛋白激酶I作为抗原虫药的化疗靶标
公开(公告)号：US20080280313A1
公开(公告)日：2008-11-13
申请号：US10586374
申请日：2005-01-12
申请人： Robert G.K. Donald , Paul Liberator , Xiaotian Zhong
发明人： Robert G.K. Donald , Paul Liberator , Xiaotian Zhong
IPC分类号： C12N9/12 , C12N15/52 , C12N15/63 , C12N1/11 , C12Q1/48 , C12P21/02
CPC分类号： C07K14/45 , C07K14/455 , C12N9/12
摘要： Isolated nucleic acid molecules encoding coccidian casein kinase I, CKI, enzymes from the species Eimeria tenella and Toxoplasma gondii are disclosed. The isolation of these coccidian CKI cDNA sequences results in the disclosure of purified forms of E. tenella and T. gondii CKI proteins, recombinant vectors and recombinant hosts which express coccidian CKI.
摘要翻译：公开了编码球虫酪蛋白激酶I，CKI，来自艾美球虫（Eimeria tenella）和弓形虫（弓形虫）的酶的分离的核酸分子。这些球虫CKI cDNA序列的分离导致纯化形式的E. tenella和弓形虫CKI蛋白，重组载体和表达球虫CKI的重组宿主的公开。

8. 发明申请

US20070178574A1 AGGRECANASE STRUCTURE 失效
公开(公告)号：US20070178574A1
公开(公告)日：2007-08-02
申请号：US11467135
申请日：2006-08-24
申请人： Lydia Mosyak , Thomas Rush , Xiaotian Zhong , Thomas McDonagh , Katy Georgiadis , Phaik-Eng Sum , Edward LaVallie , Lisa Collins-Racie , Christopher Corcoran , Ravindra Kumar , Tracy Hebert , Stephane Olland , Stewart Mackie
发明人： Lydia Mosyak , Thomas Rush , Xiaotian Zhong , Thomas McDonagh , Katy Georgiadis , Phaik-Eng Sum , Edward LaVallie , Lisa Collins-Racie , Christopher Corcoran , Ravindra Kumar , Tracy Hebert , Stephane Olland , Stewart Mackie
IPC分类号： C12N9/64 , G06F19/00
CPC分类号： C12N9/6421 , C07K2299/00 , C07K2319/00
摘要： This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.

9. 发明授权

US07693698B2 Method for identifying or designing a candidate agent that interacts with LINGO-1 polypeptide using a LINGO-1 three-dimensional structure 失效
标题翻译：使用LINGO-1三维结构识别或设计与LINGO-1多肽相互作用的候选试剂的方法
公开(公告)号：US07693698B2
公开(公告)日：2010-04-06
申请号：US11701635
申请日：2007-02-01
申请人： Lidia Mosyak , Brian Dwyer , Mark Johnson , Xiaotian Zhong , Eleonora Presman , James M. Wilhelm , Mark Stahl , William Stuart Somers
发明人： Lidia Mosyak , Brian Dwyer , Mark Johnson , Xiaotian Zhong , Eleonora Presman , James M. Wilhelm , Mark Stahl , William Stuart Somers
IPC分类号： G06G7/58 , G01N31/00
CPC分类号： C07K14/4703 , C07K14/705 , C07K2299/00
摘要： This disclosure relates to LINGO-1 polypeptides, LINGO-1 polypeptide/ligand complexes, crystals of LINGO-1 polypeptides, crystals of LINGO-1 polypeptide/ligand complexes, and related methods and software systems.
摘要翻译：本公开涉及LINGO-1多肽，LINGO-1多肽/配体复合物，LINGO-1多肽的晶体，LINGO-1多肽/配体复合物的晶体，以及相关方法和软件系统。

10. 发明授权

US07671181B2 Polynucleotides encoding coccidian parasite casein kinase I, a chemotherapeutic target for antiprotozoal agents 失效
标题翻译：编码球虫寄生虫酪蛋白激酶I的多核苷酸，一种抗原虫药的化疗靶
公开(公告)号：US07671181B2
公开(公告)日：2010-03-02
申请号：US10586374
申请日：2005-01-12
申请人： Robert G. K. Donald , Paul Liberator , Xiaotian Zhong
发明人： Robert G. K. Donald , Paul Liberator , Xiaotian Zhong
IPC分类号： C07H21/04 , C12N15/63 , C12N1/20 , C12P21/00
CPC分类号： C07K14/45 , C07K14/455 , C12N9/12
摘要： Isolated nucleic acid molecules encoding coccidian casein kinase I, CKI, enzymes from the species Eimeria tenella and Toxoplasma gondii are disclosed. The isolation of these coccidian CKI cDNA sequences results in the disclosure of purified forms of E. tenella and T. gondii CKI proteins, recombinant vectors and recombinant hosts which express coccidian CKI.
摘要翻译：公开了编码球虫酪蛋白激酶I，CKI，来自艾美球虫（Eimeria tenella）和弓形虫（弓形虫）的酶的分离的核酸分子。这些球虫CKI cDNA序列的分离导致纯化形式的E. tenella和弓形虫CKI蛋白，重组载体和表达球虫CKI的重组宿主的公开。

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