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1. 发明申请

US20090191616A1 Biosensor structure and fabricating method thereof 审中-公开
标题翻译：生物传感器结构及其制造方法
公开(公告)号：US20090191616A1
公开(公告)日：2009-07-30
申请号：US12231071
申请日：2008-08-27
申请人： Yi-Chun Lu , Tri-Rung Yew , Hwan-You Chang , Ya-Shuan Chuang
发明人： Yi-Chun Lu , Tri-Rung Yew , Hwan-You Chang , Ya-Shuan Chuang
IPC分类号： C12M1/00
CPC分类号： G01N33/48728
摘要： A biosensor structure and a method for fabricating the same are described. The biosensor structure for detecting at least a single cell includes a substrate with an insulating surface, a conductive layer and a plurality of capture molecules. The conductive layer is disposed on the substrate, and has a first pattern and a second pattern separated from each other. The first pattern includes a plurality of first finger configurations, and the second pattern includes a plurality of second finger configurations, so as to form interdigitated array. The capture molecules are immobilized on the conductive layer, such that the cell that is bound specifically to the capture molecules on two adjacent first and second finger configurations is detected. The biosensor structure is feasible for real-time (
摘要翻译：对生物传感器结构及其制造方法进行说明。用于检测至少单个电池的生物传感器结构包括具有绝缘表面的基板，导电层和多个捕获分子。导电层设置在基板上，并且具有彼此分离的第一图案和第二图案。第一图案包括多个第一手指配置，并且第二图案包括多个第二手指配置，以便形成相互指向的数组。捕获分子被固定在导电层上，使得在两个相邻的第一和第二手指配置上特异性结合捕获分子的细胞被检测。生物传感器结构对于单个细胞的实时（<3分钟），特异性和定量靶向细胞检测是可行的。

2. 发明申请

US20080108121A1 Method of arranging cells and electrode array applied thereto 审中-公开
标题翻译：布置电池和电极阵列的方法
公开(公告)号：US20080108121A1
公开(公告)日：2008-05-08
申请号：US11797418
申请日：2007-05-03
申请人： Cheng-Hsien Liu , Hwan-You Chang , Long Hsu , Chen-Ta Ho , Ruei-Zeng Lin , Wai William Wang , Ta-Yuan Lee , Chung-Cheng Chou , Chen Peng , Fung-Hsu Wu
发明人： Cheng-Hsien Liu , Hwan-You Chang , Long Hsu , Chen-Ta Ho , Ruei-Zeng Lin , Wai William Wang , Ta-Yuan Lee , Chung-Cheng Chou , Chen Peng , Fung-Hsu Wu
IPC分类号： C12N13/00 , C12M1/00
CPC分类号： C12N5/0697 , C12N5/067 , C12N2500/14 , C12N2500/16 , C12N2502/14 , C12N2502/28 , C12N2529/00 , C12N2535/10
摘要： A method of arranging cells comprises: (a) applying a voltage to two electrodes so as to allow a plurality of cells suspended in a dielectrophoresis-manipulating buffer (DEP-manipulating buffer) to be driven to be arranged into a pattern; (b) replacing the DEP-manipulating buffer with a solution comprising calcium ion and magnesium ion which helps the patterned cells adhere to the substrate; and (c) replacing the solution comprising calcium ion and magnesium ion with a medium so as to allow the patterned cells to grow on the substrate.
摘要翻译：一种布置电池的方法包括：（a）向两个电极施加电压，以允许驱动悬浮在介电电泳操纵缓冲器（DEP-操纵缓冲器）中的多个电池被布置成图案; （b）用包含钙离子和镁离子的溶液代替DEP操纵缓冲液，其帮助图案化细胞粘附到基底上; 和（c）用培养基替换包含钙离子和镁离子的溶液，以使图案化细胞在基底上生长。

3. 发明申请

US20120043209A1 MICROFLUIDIC CONTROL APPARATUS AND OPERATING METHOD THEREOF 审中-公开
标题翻译：微流控制装置及其操作方法
公开(公告)号：US20120043209A1
公开(公告)日：2012-02-23
申请号：US13212596
申请日：2011-08-18
申请人： Cheng-Hsien Liu , William Wang , Long Hsu , Yuh-Shyong Yang , Hwan-You Chang , Shih-Mo Yang , Chung-Cheng Chou
发明人： Cheng-Hsien Liu , William Wang , Long Hsu , Yuh-Shyong Yang , Hwan-You Chang , Shih-Mo Yang , Chung-Cheng Chou
IPC分类号： G01N27/447 , B01D21/00 , G01N27/453
CPC分类号： B01L3/50273 , B01D21/0009 , B01L3/502761 , B01L2200/14 , B01L2300/0816 , B01L2300/089 , B01L2400/0418 , B01L2400/082 , G01N35/1095
摘要： A microfluidic control apparatus and operating method thereof. The microfluidic control apparatus includes a photoconductive material layer and a flow passage. When a light with a specific optical pattern is emitted toward the photoconductive material layer, at least three virtual electrodes are formed on the photoconductive material layer according to the specific optical pattern. The at least three virtual electrodes include a first virtual electrode, a second virtual electrode and a third virtual electrode disposed beside the first virtual electrode. There is a specific proportion among a distance between first virtual electrode and third virtual electrode, a width of first virtual electrode, a distance between first virtual electrode and second virtual electrode, and a width of second virtual electrode. When the specific optical pattern changes, the at least three virtual electrodes also change to generate an electro-osmotic force to control the moving state of a microfluid in a flow passage.
摘要翻译：一种微流控制装置及其操作方法。微流控制装置包括光导材料层和流路。当向光导材料层发射具有特定光学图案的光时，根据特定光学图案在光导材料层上形成至少三个虚拟电极。所述至少三个虚拟电极包括设置在第一虚拟电极旁边的第一虚拟电极，第二虚拟电极和第三虚拟电极。在第一虚拟电极和第三虚拟电极之间的距离，第一虚拟电极的宽度，第一虚拟电极和第二虚拟电极之间的距离以及第二虚拟电极的宽度之间存在特定比例。当特定光学图案改变时，至少三个虚拟电极也改变以产生电渗压力以控制流路中微流体的移动状态。

4. 发明申请

US20120034635A1 METHOD FOR AMPLIFYING ADENOSINE TRIPHOSPHATE AND METHOD AND REAGENT FOR DETECTING THE CONCENTRATION OF MICROORGANISMS 审中-公开
标题翻译：用于放大二磷酸腺苷的方法和用于检测微生物浓度的方法和试剂
公开(公告)号：US20120034635A1
公开(公告)日：2012-02-09
申请号：US12954824
申请日：2010-11-26
申请人： Ching-Yi Hsu , Meng-Shun Huang , Hwan-You Chang , Hui-Ju Lee , Ming-Rong Ho
发明人： Ching-Yi Hsu , Meng-Shun Huang , Hwan-You Chang , Hui-Ju Lee , Ming-Rong Ho
IPC分类号： C12Q1/66 , C12P19/32
CPC分类号： C12P19/32 , C12Q1/04 , C12Q1/66 , C12Y113/12007 , C12Y207/02001 , C12Y207/04003 , C12Y207/07004
摘要： A method for amplifying adenosine triphosphate is provided, including mixing adenosine triphosphate sulfurylase, adenosine 5′ phosphosulfate, adenylate kinase, uridine triphosphate, acetate kinase, acetyl phosphate, luciferin and luciferase in the presence of ATP to form a mixture, and reacting the mixture to amplify ATP. A method and reagent for detecting a concentration of microorganisms are also provided.
摘要翻译：提供了一种扩增三磷酸腺苷的方法，包括在ATP存在下混合三磷酸腺苷硫酸化酶，腺苷5'磷酸硫酸，腺苷酸激酶，尿苷三磷酸，乙酸激酶，乙酰磷酸，荧光素和萤光素酶，形成混合物，放大ATP 还提供了用于检测微生物浓度的方法和试剂。

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