专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

US20130345294A1 Light-Switchable Gene Expression System 有权
标题翻译：光可切换基因表达系统
公开(公告)号：US20130345294A1
公开(公告)日：2013-12-26
申请号：US14001865
申请日：2012-02-27
申请人： Yi Yang , Xue Wang , Xianjun Chen
发明人： Yi Yang , Xue Wang , Xianjun Chen
IPC分类号： A61K48/00
CPC分类号： A61K48/0083 , C07K2319/00 , C12N15/63 , C12N15/85 , C12N2830/001
摘要： Provided is a light-switchable gene expression system, comprising: a) a recombinant light-switchable transcription factor-encoding gene, said recombinant light-switchable transcription factor comprising a first polypeptide as the DNA bonding domain, a second polypeptide as the photosensitive domain, and a third polypeptide as the transcription regulatory domain; b) a target transcription unit comprising at least one reaction element recognized and bound by the first polypeptide, a promoter regulated by the third polypeptide and a nucleotide sequence to be transcribed. Also provided is an eukaryotic expression vector comprising said light-switchable gene expression system, and a method for regulating gene expression in a host cell by using the light-switchable gene expression system. Also provided is a reagent kit containing different elements of the light-switchable gene expression system. The light-switchable gene expression system has a quick, effective and powerful induction with little or no toxicity. It is safer than other inducers, and can spatiotemporally control gene expression.
摘要翻译：提供了一种可光切换基因表达系统，其包含：a）重组可光切换转录因子编码基因，所述重组可光切换转录因子包含作为DNA结合结构域的第一多肽，作为感光结构域的第二多肽，和第三多肽作为转录调控结构域; b）靶转录单元，其包含被第一多肽识别并结合的至少一个反应元件，由第三多肽调节的启动子和待转录的核苷酸序列。还提供了包含所述可光切换基因表达系统的真核表达载体和通过使用可光切换基因表达系统调节宿主细胞中基因表达的方法。还提供了含有可光转换基因表达系统的不同元件的试剂盒。光可切换基因表达系统具有快速，有效和强大的诱导，几乎没有或没有毒性。它比其他诱导物更安全，可以时空控制基因表达。

IPRDB

热门服务

关于我们

友情链接

联系方式