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1. 发明申请

US20100248250A1 METHOD FOR PREPARING STOOL SAMPLE, SOLUTION FOR PREPARING STOOL SAMPLE, AND KIT FOR COLLECTING STOOL 有权
标题翻译：制备样品样品的方法，制备样品的方法和收集STOOL的工具箱
公开(公告)号：US20100248250A1
公开(公告)日：2010-09-30
申请号：US12782537
申请日：2010-05-18
申请人： Yasuo Tanigami , Tomonori Nagaoka
发明人： Yasuo Tanigami , Tomonori Nagaoka
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , G01N1/38
摘要： Provided in the present invention are a method for preparing a stool sample without any need for complicated operations which is capable of efficiently recovering a nucleic acid or the like originating from mammalian cells, such as the cells exfoliated from the large intestine, in the stool; a solution for preparing a stool sample and a kit for stool collection which are used in the above method; and a method for recovering and analyzing the nucleic acid in stool using the stool sample prepared by the above method. More specifically, provided are a method for preparing a stool sample which is a method for preparing a stool sample in order to efficiently recover a nucleic acid from the stool sample, the method characterized in that the collected stool is mixed with a solution for preparing a stool sample which has a water-soluble organic solvent as an active ingredient; a solution for preparing a stool sample which is used in the method; a kit for collecting stool; a method for recovering a nucleic acid characterized by recovering a nucleic acid originating from indigenous enteric bacterium and a nucleic acid originating from an organism other than indigenous enteric bacterium at the same time from the stool sample prepared by the preparation method; and a method for analyzing a nucleic acid that analyzes the nucleic acid recovered by the method for recovering a nucleic acid.
摘要翻译：在本发明中提供了一种制备粪便样品的方法，不需要复杂的操作，其能够有效地回收源自哺乳动物细胞的核酸等，例如在大肠中从大肠剥离的细胞; 用于制备粪便样品的溶液和用于上述方法的粪便收集试剂盒; 以及使用通过上述方法制备的粪便样品来回收和分析粪便中的核酸的方法。更具体地，提供了一种制备粪便样品的方法，该方法是用于制备粪便样品的方法，以便从粪便样品中有效地回收核酸，该方法的特征在于将收集的粪便与制备粪便样品，其具有水溶性有机溶剂作为活性成分; 用于制备用于该方法的粪便样品的溶液; 用于收集粪便的工具箱一种回收核酸的方法，其特征在于从通过制备方法制备的粪便样品中同时回收源自原生肠细菌的核酸和源自本土肠细菌以外的生物的核酸; 以及分析通过回收核酸的方法回收的核酸的核酸的分析方法。

2. 发明授权

US08754204B2 Method for preparing stool sample, solution for preparing stool sample, and kit for collecting stool 有权
标题翻译：粪便样品的制备方法，粪便样品的制备方法以及收集粪便的试剂盒
公开(公告)号：US08754204B2
公开(公告)日：2014-06-17
申请号：US12782537
申请日：2010-05-18
申请人： Yasuo Tanigami , Tomonori Nagaoka
发明人： Yasuo Tanigami , Tomonori Nagaoka
IPC分类号： C07H21/00 , C07H21/02
CPC分类号： C12Q1/6806 , G01N1/38
摘要： A method for preparing a stool sample without any need for complicated operations is provided which is capable of efficiently recovering a nucleic acid originating from mammalian cells, such as the cells exfoliated from the large intestine, in the stool. A solution for preparing a stool sample and a kit for stool collection are also provided. The collected stool is mixed with a solution for preparing a stool sample which has a water-soluble organic solvent as an active ingredient. A method is disclosed for recovering a nucleic acid including recovering a nucleic acid originating from indigenous enteric bacterium and a nucleic acid originating from an organism other than indigenous enteric bacterium at the same time from the stool sample prepared by the preparation method.
摘要翻译：提供一种制备粪便样品而不需要复杂操作的方法，其能够有效地回收来自哺乳动物细胞的核酸，例如在大肠中从大肠剥离的细胞。还提供了一种用于制备粪便样品和用于粪便收集的试剂盒的溶液。将收集的粪便与制备具有水溶性有机溶剂作为活性成分的粪便样品的溶液混合。公开了一种用于回收核酸的方法，包括从制备方法制备的粪便样品中同时回收源自原生肠细菌的核酸和源自本土肠细菌以外的生物体的核酸。

3. 发明申请

US20110236895A1 METHOD FOR PREPARING SAMPLE, SOLUTION FOR PREPARING SAMPLE AND STOOL COLLECTION KIT METHOD FOR ANALYZING A NUCLEIC ACID 审中-公开
标题翻译：制备样品的方法，制备用于分析核酸的样品和STOOL收集试剂盒方法的方法
公开(公告)号：US20110236895A1
公开(公告)日：2011-09-29
申请号：US13153045
申请日：2011-06-03
申请人： Yasuo Tanigami , Tomonori Nagaoka
发明人： Yasuo Tanigami , Tomonori Nagaoka
IPC分类号： C12Q1/68 , C07H1/08 , C09K15/22 , C09K15/20 , C09K15/06
CPC分类号： C12N15/1003 , A61B10/0038 , A61B10/007 , A61B10/0096 , C12Q1/6806 , C12Q2527/125
摘要： The present invention relates to the providing of a method for preparing a sample from a nucleic acid-containing sample such as biological samples, where inhibitory substance's action against a enzyme reaction using a nucleic acid as substrate are decreased, a solution for preparing a sample used for the method, a stool collection kit used in that method, and a method for recovering and analyzing a nucleic acid in a nucleic acid-containing sample using a sample prepared using the preparation method of the present invention. A method for preparing a sample according to the present invention is a method for preparing a sample being used for analyzing a nucleic acid, and is characterized in that a nucleic acid-containing sample is mixed with a solution having one or more members selected from the group consisting of a polycation and a chelating agent as an active ingredient.
摘要翻译：本发明涉及提供从含有核酸的样品（例如生物样品）制备样品的方法，其中抑制物质对使用核酸作为底物的酶反应的作用降低，制备使用的样品的溶液用于该方法的方法中使用的粪便收集工具，以及使用本发明的制备方法制备的样品来回收和分析含核酸样品中的核酸的方法。根据本发明的制备样品的方法是用于制备用于分析核酸的样品的方法，其特征在于，将含有核酸的样品与选自以下的一种或多种成分的溶液混合：由聚阳离子和螯合剂作为活性成分组成的组。

4. 发明申请

US20110183332A1 METHOD FOR RECOVERING NUCLEIC ACID FROM STOOL SAMPLE, NUCLEIC ACID ANALYSIS METHOD AND STOOL SAMPLE PROCESSING APPARATUS 审中-公开
标题翻译：从STOOL样品中回收核酸的方法，核酸分析方法和STOOL样品加工设备
公开(公告)号：US20110183332A1
公开(公告)日：2011-07-28
申请号：US13012442
申请日：2011-01-24
申请人： Tomonori Nagaoka , Yasuo Tanigami
发明人： Tomonori Nagaoka , Yasuo Tanigami
IPC分类号： C12Q1/68 , C07H1/06 , C07H21/00 , G01N33/48
CPC分类号： C12N15/1003 , C12Q1/6806 , C12Q1/6883 , C12Q2527/101 , C12Q2527/125 , C12Q2600/154
摘要： The present invention relates to the a nucleic acid analysis method that uses nucleic acid recovered according to the nucleic acid recovery method, and a stool sample processing apparatus used in that method, wherein the method has steps of: (A) eluting nucleic acid amplification reaction inhibitory substances by preparing a stool sample by adding a collected stool to a solution for removing nucleic acid amplification reaction inhibitory substances that has a water-soluble organic solvent as an active ingredient thereof, and storing the stool sample for a predetermined time, (B) recovering a stool-derived solid fraction by removing the solution for removing nucleic acid amplification reaction inhibitory substances from the stool sample after step (A), and (C) recovering nucleic acid from the stool-derived solid fraction recovered in step (B).
摘要翻译：本发明涉及使用根据核酸回收方法回收的核酸的核酸分析方法和该方法中使用的粪便样品处理装置，其中，所述方法具有以下步骤：（A）洗脱核酸扩增反应（B）将粪便样品添加到用于除去具有水溶性有机溶剂的活性成分的核酸扩增反应抑制物质的溶液中，通过从步骤（A）中的粪便样品中除去用于除去核酸扩增反应抑制物质的溶液来回收粪便衍生的固体部分，和（C）从步骤（B）中回收的粪便衍生的固体部分中回收核酸。

5. 发明申请

US20120064535A1 METHOD OF PREPARING SAMPLES CONTAINING NUCLEIC ACIDS 审中-公开
标题翻译：制备含有核酸的样品的方法
公开(公告)号：US20120064535A1
公开(公告)日：2012-03-15
申请号：US13300006
申请日：2011-11-18
申请人： Yasuo Tanigami , Tomonori Nagaoka
发明人： Yasuo Tanigami , Tomonori Nagaoka
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12N15/1003 , C12Q1/6806 , C12Q2527/125 , C12Q2527/119
摘要： The present invention provides a method of preparing a sample enabling efficient recovery of nucleic acids from a biological sample such as stool without requiring a bothersome procedure. The method of preparing a nucleic acid-containing sample of the present invention comprises (A) a step for mixing a biological sample with a nucleic acid stabilizer, (B) a step for recovering a solid component from the mixture obtained in step (A) in the form of a nucleic acid-containing sample, and (C) a step for washing the solid component recovered in step (B) using an acidic buffer solution having a pH of 2 or higher.
摘要翻译：本发明提供一种制备样品的方法，其能够有效地从生物样品如粪便中回收核酸，而不需要麻烦的程序。制备本发明的含核酸样品的方法包括（A）将生物样品与核酸稳定剂混合的步骤，（B）从步骤（A）获得的混合物中回收固体组分的步骤，以（c）使用pH为2以上的酸性缓冲溶液洗涤在步骤（B）中回收的固体成分的步骤。

6. 发明申请

US20120064525A1 METHOD FOR COLLECTION OF NUCLEIC ACID DERIVED FROM MAMMALIAN CELL, METHOD FOR ANALYSIS OF NUCLEIC ACID, AND KIT FOR COLLECTION OF FECES 审中-公开
标题翻译：用于收集从马马里亚细胞衍生的核酸的方法，用于分析核酸的方法和用于收集FEC的试剂盒
公开(公告)号：US20120064525A1
公开(公告)日：2012-03-15
申请号：US13300123
申请日：2011-11-18
申请人： Masanori Asakura , Tomonori Nagaoka , Yasuo Tanigami
发明人： Masanori Asakura , Tomonori Nagaoka , Yasuo Tanigami
IPC分类号： C12Q1/68 , C07H21/00 , C12M1/00 , C07H1/08
CPC分类号： C12N15/1003 , C12Q1/6806 , C12Q1/6883 , C12Q2600/158 , Y02A50/451 , C12Q2527/125
摘要： A method of recovering a nucleic acid derived from a mammalian cell taken from feces is provided. The nucleic acid is recovered easily even from feces collected in routine health checkups or the like. The nucleic acid of mammalian cell can be recovered more selectively than that from an enterobacterial cell. The method of recovering of the present invention includes the steps of, (A) preparing a fecal sample by placing the feces in a treatment solution with a high salt concentration, immersing the feces in the treatment solution for a predetermined period; (B) recovering a solid component from the fecal sample after the step (A); and (C) recovering a nucleic acid from the solid component recovered in the step (B).
摘要翻译：提供了从粪便中回收源自哺乳动物细胞的核酸的方法。即使从常规健康检查等中收集的粪便中也可以容易地回收核酸。哺乳动物细胞的核酸可以比来自肠细菌细胞的核酸更有选择地被回收。本发明的回收方法包括以下步骤：（A）通过将粪便置于高盐浓度的处理溶液中制备粪便样品，将粪便浸入处理溶液中预定的时间; （B）在步骤（A）之后从粪便样品中回收固体组分; 和（C）从步骤（B）中回收的固体成分回收核酸。

7. 发明申请

US20110244461A1 METHOD FOR PREPARING STOOL SAMPLE, SOLUTION FOR PREPARING STOOL SAMPLE AND STOOL COLLECTION KIT 审中-公开
标题翻译：制备样品样品的方法，用于制备样品样品和STOOL收集试剂盒的方法
公开(公告)号：US20110244461A1
公开(公告)日：2011-10-06
申请号：US13152908
申请日：2011-06-03
申请人： Yasuo Tanigami , Tomonori Nagaoka
发明人： Yasuo Tanigami , Tomonori Nagaoka
IPC分类号： C12Q1/68 , C07H1/08
CPC分类号： C12Q1/6806 , A61B10/0038 , C12Q1/6883 , C12Q2527/127 , C12Q2527/125 , C12Q2521/537
摘要： The present invention relates to the providing of a method for preparing a stool sample that enables a nucleic acid in a stool to be stably preserved without requiring a complex procedure, a solution for preparing a stool sample, a stool collection kit used in that method, and a method for recovering and analyzing a nucleic acid in a stool using a stool sample prepared using the preparation method of the present invention. A method for preparing a stool sample according to the present invention is a method for preparing a stool sample being used for analyzing a nucleic acid contained in the stool, and is characterized in that a collected stool is mixed with a solution having a protease inhibitor as an active ingredient.
摘要翻译：本发明涉及提供一种制备粪便样品的方法，其使粪便中的核酸能够稳定地保持而不需要复杂的程序，制备粪便样品的溶液，该方法中使用的粪便收集试剂盒，以及使用使用本发明的制备方法制备的粪便样品来回收和分析粪便中的核酸的方法。根据本发明的制备粪便样品的方法是一种用于分析粪便中所含核酸的粪便样品的制备方法，其特征在于将收集的粪便与具有蛋白酶抑制剂的溶液混合活性成分。

8. 发明申请

US20110189673A1 STOOL SAMPLE PREPARATION METHOD, SOLUTION FOR PREPARING STOOL SAMPLE AND STOOL COLLECTION KIT 审中-公开
标题翻译： STOOL样品制备方法，用于制备STOOL样品和STOOL收集包的方法
公开(公告)号：US20110189673A1
公开(公告)日：2011-08-04
申请号：US13034220
申请日：2011-02-24
申请人： Yasuo Tanigami , Tomonori Nagaoka
发明人： Yasuo Tanigami , Tomonori Nagaoka
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6806 , C12Q2527/125
摘要： The present invention relates to the providing of a method for preparing a stool sample that enables nucleic acids in a stool to be stably preserved without requiring a complex procedure, a stool sample preparation solution and stool collection kit used in that method, and a method for recovering and analyzing nucleic acids in a stool using a stool sample prepared according to the preparation method of the present invention, and a stool sample having superior preservation of nucleic acids contained in the stool sample is prepared by mixing a collected stool with the stool sample preparation solution having for an active ingredient thereof a water-soluble organic solvent containing an organic acid.
摘要翻译：本发明涉及提供一种制备粪便样品的方法，其使粪便中的核酸能够稳定地保持而不需要复杂的程序，该方法中使用的粪便样品制备溶液和粪便收集试剂盒，以及用于使用根据本发明的制备方法制备的粪便样品来回收和分析粪便中的核酸，并且通过将收集的粪便与粪便样品制剂混合来制备具有对粪便样品中所含的核酸的优异保存的粪便样品对其活性成分具有含有机酸的水溶性有机溶剂的溶液。

9. 发明申请

US20070099221A1 Method of analyzing nucleic acid 审中-公开
标题翻译：分析核酸的方法
公开(公告)号：US20070099221A1
公开(公告)日：2007-05-03
申请号：US11588561
申请日：2006-10-27
申请人： Tomonori Nagaoka
发明人： Tomonori Nagaoka
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6832 , C12Q2545/101
摘要： An object of the invention is to avoid any possibility of error caused by a nucleic acid used as a standard, a probe, or probe spot, etc. or enable correction thereof, to analyze the frequency of gene expressions with high reproducibility, and to analyze any change of primary structure in a sample nucleic acid. The invention provides a method of analyzing the primary structure of at least one target base sequence present in a sample nucleic acid by detecting any signal from a sample nucleic acid hybrid composed of a probe and the same nucleic acid, wherein any signal from a synthetic standard nucleic acid hybrid composed of the probe and a synthetic standard nucleic acid containing at least one target base sequence is detected and on the basis of the signal from the synthetic standard nucleic acid hybrid, the signal from the sample nucleic acid hybrid is corrected to thereby effect analysis of the primary structure of the sample nucleic acid.
摘要翻译：本发明的目的是避免由用作标准的核酸，探针或探针斑点等引起的错误的任何可能性或能够进行校正，以高再现性分析基因表达的频率，并分析样品核酸中一级结构的任何变化。本发明提供了一种通过检测由探针和相同核酸组成的样品核酸杂交的任何信号来分析样品核酸中存在的至少一种靶碱基序列的一级结构的方法，其中来自合成标准检测由探针构成的核酸杂交体和含有至少一个靶基序列的合成标准核酸，根据来自合成标准核酸杂交体的信号，对来自样品核酸杂交体的信号进行校正，从而实现分析样品核酸的一级结构。

10. 发明申请

US20100255481A1 METHOD FOR DETECTION OF ADENOMA OR CANCER BY GENETIC ANALYSIS 审中-公开
标题翻译：通过遗传分析检测腺瘤或癌症的方法
公开(公告)号：US20100255481A1
公开(公告)日：2010-10-07
申请号：US12740283
申请日：2008-10-30
申请人： Tamao Akesaka , Tomonori Nagaoka , Shigeru Kanaoka , Kenichi Yoshida
发明人： Tamao Akesaka , Tomonori Nagaoka , Shigeru Kanaoka , Kenichi Yoshida
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16
摘要： Disclosed is a method which enables an early stage detection of an adenoma or cancer by gene expression analysis of a biomarker in a readily collectable sample. Specifically disclosed is a method for detecting an adenoma or cancer, which comprises the steps of: measuring the quantity of a sequence constituting at least one housekeeping gene or an expression product thereof contained in a body fluid sample or an excrement sample collected from an examinee; and calculating the concentration of the sequence in the sample.
摘要翻译：公开了一种能够通过对容易收集的样品中的生物标志物的基因表达分析来进行腺瘤或癌症的早期检测的方法。具体公开的是检测腺瘤或癌症的方法，其特征在于，包括以下步骤：测定构成体液样本中所含的至少一个持家基因或其表达产物的序列的量，或从受检者收集的排泄物样品; 并计算样品中序列的浓度。

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