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1. 发明申请

US20090068728A1 Cell incubator 审中-公开
标题翻译：细胞培养箱
公开(公告)号：US20090068728A1
公开(公告)日：2009-03-12
申请号：US12230258
申请日：2008-08-26
申请人： Yasujiro KIYOTA , Takayuki Uozumi
发明人： Yasujiro KIYOTA , Takayuki Uozumi
IPC分类号： C12M3/00
CPC分类号： C12M41/14 , C12M41/36 , G02B7/38
摘要： A cell incubator having an observation unit to observe a cell incubation process comprises: a focus detecting unit to detect a focus position of an imaging optical system of the observation unit; an optimum focus selecting unit to select an optimum focus position based upon a plurality of images taken by moving the imaging optical system stepwise at a fine pitch along an optical axis direction from the focus position, based upon the focus position detected by the focus detecting unit; and a fluorescence observation unit to shoot a fluorescence image of the cells by moving the imaging optical system to the optimum focus position thus detected by the optimum focus selecting unit.
摘要翻译：具有用于观察细胞培养过程的观察单元的细胞培养箱包括：焦点检测单元，用于检测观察单元的成像光学系统的焦点位置; 最佳焦点选择单元，基于由焦点检测单元检测到的对焦位置，基于通过沿着光轴方向以微小间距逐渐地从聚焦位置逐步移动成像光学系统而获取的多个图像来选择最佳聚焦位置; ; 以及荧光观察单元，通过将成像光学系统移动到由最佳聚焦选择单元检测到的最佳聚焦位置来拍摄细胞的荧光图像。

2. 发明申请

US20130027539A1 CELL OBSERVING APPARATUS AND CELL INTEGRATION METHOD 有权
标题翻译：细胞观察装置和细胞整合方法
公开(公告)号：US20130027539A1
公开(公告)日：2013-01-31
申请号：US13552839
申请日：2012-07-19
申请人： Yasujiro KIYOTA , Takayuki Uozumi
发明人： Yasujiro KIYOTA , Takayuki Uozumi
IPC分类号： H04N7/18
CPC分类号： C12M41/36 , G02B21/0088 , G02B21/32 , G02B21/36
摘要： User's time and labor required for performing manipulations with respect to cells which exist in an incubation container, are reduced. To achieve the above, a cell observing apparatus includes an observation stage supporting an incubation container that houses cells, a micro imaging optical system forming, on an imaging device for micro imaging, an image of the cell in the incubation container disposed at an observing position of the observation stage, a macro imaging optical system forming, on an imaging device for macro imaging, an image of an area wider than that captured by the micro imaging optical system in the incubation container, and a controlling unit controlling an operation of a manipulation needle that manipulates the cells in the incubation container, in which the micro imaging optical system is disposed on a side facing the macro imaging optical system with the observation stage being located therebetween.
摘要翻译：对于存在于孵育容器中的细胞进行操作所需的用户时间和劳动力减少。为了实现上述目的，细胞观察装置包括：支持容纳细胞的培养容器的观察台，在微成像用成像装置上形成在设置在观察位置的培养容器中的细胞的图像的微成像光学系统在宏观成像装置上形成比由培养容器中的微成像光学系统捕获的区域宽的区域的图像的宏观成像光学系统，以及控制操作的控制单元操纵培养容器中的细胞的针，其中微成像光学系统设置在面向宏观成像光学系统的一侧，观察台位于其间。

3. 发明申请

US20120214150A1 STIRRING METHOD, CELL CULTURE METHOD, STIRRING APPARATUS, AND CELL CULTURE APPARATUS 审中-公开
标题翻译：刺激方法，细胞培养方法，刺激装置和细胞培养装置
公开(公告)号：US20120214150A1
公开(公告)日：2012-08-23
申请号：US13401051
申请日：2012-02-21
申请人： Yasujiro KIYOTA
发明人： Yasujiro KIYOTA
IPC分类号： C12Q3/00 , C12M1/36
CPC分类号： C12M27/16 , C12M33/08 , C12M41/14 , C12M41/36 , C12M41/42 , C12M41/48
摘要： A stirring method includes measuring, with respect to cells which adhere to an inner surface of a culture vessel to be cultured, an ease of peeling between the culture vessel and the cell and an ease of peeling between the mutual cells, and performing a stirring process on the cells in the culture vessel by determining a process content of the stirring process with respect to the cells in the culture vessel, based on the ease of peeling between the culture vessel and the cell and the ease of peeling between the mutual cells.
摘要翻译：一种搅拌方法，其特征在于，相对于附着于待培养的培养容器的内表面的细胞，测定培养容器与细胞之间的剥离容易性以及相互细胞之间的剥离容易性，进行搅拌处理基于培养容器和细胞之间的剥离容易性和相互细胞之间的剥离容易性，通过确定培养容器中的细胞的搅拌过程的工艺含量，在培养容器中的细胞上。

4. 发明申请

US20120194664A1 CELL PICKING-ASSISTING DEVICE, DISPLAY DEVICE AND CULTURE CONTAINER 有权
标题翻译：细胞拾取辅助装置，显示装置和文化容器
公开(公告)号：US20120194664A1
公开(公告)日：2012-08-02
申请号：US13344038
申请日：2012-01-05
申请人： Yasujiro KIYOTA
发明人： Yasujiro KIYOTA
IPC分类号： H04N7/18
CPC分类号： C12M47/04 , C12M23/50 , C12M41/46 , C12M41/48 , G06F19/00
摘要： A cell picking support device, a display device and a culture container that can enhance work efficiency. An adapter fixes a well plate that holds cells cultured in a cell culture observation device. A control unit reads culture observation information that includes cell information and coordinate information, from a memory card that is fitted to an external memory I/F. A display unit is provided on a placing surface side of the well plate that is fixed by the adapter, and in response to control by the control unit, displays the cell information, read from the memory card, at display positions corresponding to positions on the bottom face of the well plate, as specified by the coordinate information. Embodiments can be used, for instance, as a cell picking support device that supports a picking operation that is performed in a clean bench.
摘要翻译：可提高工作效率的细胞采集支持装置，显示装置和培养容器。适配器固定保持在细胞培养观察装置中培养的细胞的孔板。控制单元从安装到外部存储器I / F的存储卡中读取包括单元信息和坐标信息的文化观察信息。显示单元设置在由适配器固定的孔板的放置表面侧上，并且响应于控制单元的控制，将从存储卡读取的单元信息显示在对应于位置的显示位置井板底面，由坐标信息指定。实施例可以用作例如支持在洁净工作台中执行的拾取操作的细胞采集支持装置。

5. 发明申请

US20100208960A1 CULTURE APPARATUS, CULTURE INFORMATION MANAGEMENT METHOD, AND COMPUTER READABLE MEDIUM STORING PROGRAM OF SAME 有权
标题翻译：文化设备，文化信息管理方法和计算机可读介质存储程序
公开(公告)号：US20100208960A1
公开(公告)日：2010-08-19
申请号：US12709017
申请日：2010-02-19
申请人： Yasujiro KIYOTA
发明人： Yasujiro KIYOTA
IPC分类号： G06K9/00
CPC分类号： C12M41/48 , C12M41/14 , G06F19/00
摘要： A temperature-controlled room accommodates an incubation container incubating a sample and maintains the inside thereof in a predetermined environment condition. An imaging section generates data of a plurality of observing images. An image analyzing section performs an image analyzing process on the data of the plurality of observing images obtained by imaging the incubation container at different times. Image analyzing data generated by the image analyzing section includes morphological information which indicates incubation states of the samples and rate-of-change information regarding the morphological information. A search processing section conducts a search for the incubation container which meets the first searching condition and the second searching condition based on the image analyzing data.
摘要翻译：温度控制室容纳孵育容器，孵育样品并将其内部保持在预定的环境条件下。成像部分生成多个观察图像的数据。图像分析部对不同时间对培养容器进行成像而获得的多个观察图像的数据进行图像分析处理。由图像分析部生成的图像分析数据包括表示样本的孵育状态的形态信息和关于形态信息的变化率信息。搜索处理部分基于图像分析数据进行满足第一搜索条件和第二搜索条件的孵化容器的搜索。

6. 发明申请

US20120120302A1 FOCUS CONTROL METHOD AND CULTURE OBSERVATION APPARATUS 有权
标题翻译：重点控制方法和文化观察装置
公开(公告)号：US20120120302A1
公开(公告)日：2012-05-17
申请号：US13290565
申请日：2011-11-07
申请人： Yasujiro KIYOTA , Yoichi WADA , Hiroaki KII
发明人： Yasujiro KIYOTA , Yoichi WADA , Hiroaki KII
IPC分类号： G02B21/02 , G02B21/36 , G02B21/26 , G03B13/32 , G02B21/00
CPC分类号： G02B21/244 , C12M21/06 , C12M25/01 , C12M41/14 , C12M41/36 , G02B7/38
摘要： The focus control culture observation apparatus enable simple and reliable detection of a focusing position of an objective lens on a subject and with certainty. When a focusing position is detected, an area, which includes an edge portion of the culture medium drop is selected as an AF area which is to be a target of detecting the focusing position of the objective lens on the culture medium drop. The apparatus detects the focusing position on the culture medium drop based on an observation image of the AF area. By detecting the focusing position as a targeting area that is different from an area which is near the edge of the culture container and where illumination unevenness is generated, the detection accuracy can be improved.
摘要翻译：聚焦控制文化观察装置能够确实地对被摄体上的物镜的聚焦位置进行简单可靠的检测。当检测到聚焦位置时，选择包括培养基液滴的边缘部分的区域作为将物镜在培养基液滴上检测聚焦位置的对象的AF区域。该装置基于AF区域的观察图像来检测培养基落下的聚焦位置。通过将聚焦位置检测为与培养容器的边缘附近的区域不同的目标区域，并且产生照明不均匀，可以提高检测精度。

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