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1. 发明授权

US07510735B2 Method of evaluating the extent of reaching the intestine of bifidobacterium in fermented milk food or drink 失效
标题翻译：评估在发酵乳食品或饮料中到达双歧杆菌肠的程度的方法
公开(公告)号：US07510735B2
公开(公告)日：2009-03-31
申请号：US10470776
申请日：2001-12-21
申请人： Yasuhisa Shimakawa , Masaki Serata , Hirokazu Tsuji , Koichiro Sonoike , Akimitsu Takagi , Mika Miura , Fumiyasu Ishikawa
发明人： Yasuhisa Shimakawa , Masaki Serata , Hirokazu Tsuji , Koichiro Sonoike , Akimitsu Takagi , Mika Miura , Fumiyasu Ishikawa
IPC分类号： A23C9/12 , A23C17/00
CPC分类号： C12Q1/18 , A23C9/1234 , A23Y2300/00 , C12Q1/025
摘要： A Bifidobacterium that is resistant to acid and bile and which retains at least 20% viability after acid and bile treatment. Foods and drinks containing an acid- and bile-resistant Bifidobacterium. Methods for providing viable Bifidobacteria to the intestine after ingestion of a food or drink containing an acid- and bile-resistant Bifidobacterium.
摘要翻译：一种对酸和胆汁有抗性且在酸和胆汁处理后保持至少20％活力的双歧杆菌。食品和饮料含有耐酸和胆汁的双歧杆菌。在摄入含有耐酸碱性和双歧杆菌双歧杆菌的食物或饮料后，向肠中提供活菌的方法。

2. 发明授权

US07794763B2 Bacterium of the genus bifidobacterium and fermented foods using the same 有权
标题翻译：双歧杆菌属的细菌和使用其的发酵食品
公开(公告)号：US07794763B2
公开(公告)日：2010-09-14
申请号：US10493068
申请日：2002-11-01
申请人： Masaki Serata , Hirokazu Tsuji , Koichiro Sonoike
发明人： Masaki Serata , Hirokazu Tsuji , Koichiro Sonoike
IPC分类号： A23C9/12
CPC分类号： C12R1/01 , A23C9/1234 , A23Y2300/29
摘要： The present invention provides a bacterium belonging to the genus Bifidobacterium, which can exhibit a high viability in the storage under agitation and can produce a fermented food containing a large number of viable bacterial count. The present invention further provides a fermented food, which contains the bacterium belonging to the genus Bifidobacterium and exhibits various physiological effects such as inhibitory action against harmful intestinal bacteria and intestinal function controlling effects. The present invention further provides a bacterium belonging to the genus Bifidobacterium having a rate of viability of 30% or more, when the bacterium is stored in the aerobic condition under agitation at 10° C. for 14 days after culturing in a medium containing milk as a main ingredient until obtaining a viable count of 1×108 cfu/ml or more, and fermented food using the same.
摘要翻译：本发明提供了属于双歧杆菌属的细菌，其可以在搅拌下储存中显示出高活力性，并且可以生产含有大量活菌数的发酵食品。本发明还提供一种发酵食品，其含有属于双歧杆菌属的细菌，并且具有各种生理效果，例如对肠道细菌有害的抑制作用和肠功能控制作用。本发明还提供了一种属于双歧杆菌属的细菌，当细菌在需氧条件下在10℃搅拌下储存14天后，在含有牛奶的培养基中培养14天后，具有30％或更高的存活率是获得1×10 8 cfu / ml以上的活菌数量的主要成分，以及使用其的发酵食品。

3. 发明申请

US20050031735A1 Bacterium of the genus bifidobacterium and fermented foods using the same 有权
标题翻译：双歧杆菌属的细菌和使用其的发酵食品
公开(公告)号：US20050031735A1
公开(公告)日：2005-02-10
申请号：US10493068
申请日：2002-11-01
申请人： Masaki Serata , Hirokazu Tsuji , Koichiro Sonoike
发明人： Masaki Serata , Hirokazu Tsuji , Koichiro Sonoike
IPC分类号： A23C9/123 , A23C9/12
CPC分类号： C12R1/01 , A23C9/1234 , A23Y2300/29
摘要： The present invention provides a bacterium belonging to the genus Bifidobacterium, which can exhibit a high viability in the storage under agitation and can produce a fermented food containing a large number of viable bacterial count. The present invention further provides a fermented food, which contains the bacterium belonging to the genus Bifidobacterium and exhibits various physiological effects such as inhibitory action against harmful intestinal bacteria and intestinal function controlling effects. The present invention further provides a bacterium belonging to the genus Bifidobacterium having a rate of viability of 30% or more, when the bacterium is stored in the aerobic condition under agitation at 10° C. for 14 days after culturing in a medium containing milk as a main ingredient until obtaining a viable count of 1×108 cfu/ml or more, and fermented food using the same.
摘要翻译：本发明提供了属于双歧杆菌属的细菌，其可以在搅拌下储存中显示出高活力性，并且可以生产含有大量活菌数的发酵食品。本发明还提供一种发酵食品，其含有属于双歧杆菌属的细菌，并且具有各种生理效果，例如对肠道细菌有害的抑制作用和肠功能控制作用。本发明还提供了一种属于双歧杆菌属的细菌，当细菌在需氧条件下在10℃搅拌下储存14天后，在含有牛奶的培养基中培养14天后，具有30％或更高的存活率是获得1×10 8 cfu / ml以上的活菌数量的主要成分，以及使用其的发酵食品。

4. 发明授权

US09139837B2 Hydrogen peroxide resistance-imparting gene and method for using same 有权
标题翻译：耐过氧化氢赋予基因及其使用方法
公开(公告)号：US09139837B2
公开(公告)日：2015-09-22
申请号：US14003094
申请日：2012-03-02
申请人： Masaki Serata , Mayumi Kiwaki , Tohru Iino
发明人： Masaki Serata , Mayumi Kiwaki , Tohru Iino
IPC分类号： C12N15/63 , C12N15/74 , C07K14/335 , C12Q1/68 , A61K35/747 , A61K35/00
CPC分类号： C12N15/746 , A61K35/747 , A61K2035/115 , C07K14/335 , C12Q1/689 , C12Q2600/158
摘要： Provided is a gene that is useful for imparting hydrogen peroxide resistance to a microorganism. Also provided is a method for using the same. The hydrogen peroxide resistance-imparting gene encoding a protein selected from the following proteins (a) to (c): (a) a protein having the amino acid sequence of SEQ ID NO: 2; (b) a protein which has an amino acid sequence equivalent to the amino acid sequence of (a), except that one to several amino acid residues are deleted, substituted, or added, and which exhibits hydrogen peroxide resistance-imparting activity; and (c) a protein which has an amino acid sequence having an identity of 85% or higher to the amino acid sequence of (a), and which exhibits hydrogen peroxide resistance-imparting activity.
摘要翻译：提供可用于赋予微生物过氧化氢抗性的基因。还提供了使用该方法的方法。编码选自以下蛋白质（a）〜（c）的蛋白质的过氧化氢赋予性基因：（a）具有SEQ ID NO：2的氨基酸序列的蛋白质; （b）具有与（a）的氨基酸序列等同的氨基酸序列的蛋白质，除了1〜数个氨基酸残基被缺失，取代或添加，并且具有赋予过氧化氢赋予活性的蛋白质。和（c）具有与（a）的氨基酸序列同一性为85％以上的氨基酸序列的蛋白质，其具有赋予过氧化氢的赋予活性。

5. 发明授权

US08404823B2 Cytokine production regulator gene and use thereof 有权
标题翻译：细胞因子生产调节基因及其用途
公开(公告)号：US08404823B2
公开(公告)日：2013-03-26
申请号：US12447370
申请日：2007-10-26
申请人： Tomoyuki Sako , Emi Yasuda , Masaki Serata , Satoshi Matsumoto , Kan Shida
发明人： Tomoyuki Sako , Emi Yasuda , Masaki Serata , Satoshi Matsumoto , Kan Shida
IPC分类号： C07H21/02 , C12P1/00 , C12Q1/68 , C12N1/20 , C12N15/00 , C12N15/74 , C40B40/00
CPC分类号： C12N15/52 , A23L33/135 , A61K38/00 , C12N9/1048 , C12P19/04
摘要： The invention provides a gene encoding a protein selected from among the following proteins (a) to (c): (a) a protein having any of the amino acid sequences of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, and 108; (b) a protein which has an amino acid sequence equivalent to any of the amino acid sequences of (a), except that one to several amino acid residues are deleted, substituted, or added, and which exhibits cytokine production regulatory activity; and (c) a protein which has an amino acid sequence having 90% or higher identity to any of the amino acid sequences of (a), and which exhibits cytokine production regulatory activity, as well as a gene useful for regulating cytokine production and use of the gene.
摘要翻译：本发明提供了编码选自以下蛋白质（a）〜（c）的蛋白质的基因：（a）具有SEQ ID NO：2,4,6,8,10,12的任意氨基酸序列的蛋白质，14，16，82，84，86，88，90，92，94，96，98，100，102，104，106及108; （b）具有与（a）的任何氨基酸序列相同的氨基酸序列的蛋白质，除了一至数个氨基酸残基被缺失，取代或添加，并且其表现出细胞因子产生调节活性; 和（c）具有与（a）的任何氨基酸序列具有90％或更高同一性并且表现出细胞因子产生调节活性的氨基酸序列的蛋白质，以及可用于调节细胞因子产生和使用的基因的基因。

6. 发明申请

US20100144551A1 CYTOKINE PRODUCTION REGULATOR GENE AND USE THEREOF 有权
标题翻译：细胞因子生产调节因子基因及其用途
公开(公告)号：US20100144551A1
公开(公告)日：2010-06-10
申请号：US12447370
申请日：2007-10-26
申请人： Tomoyuki Sako , Emi Yasuda , Masaki Serata , Satoshi Matsumoto , Kan Shida
发明人： Tomoyuki Sako , Emi Yasuda , Masaki Serata , Satoshi Matsumoto , Kan Shida
IPC分类号： C40B40/08 , C07H21/00 , C12N15/74 , C12N1/00 , C12N1/21 , C12Q1/68 , C12N15/63
CPC分类号： C12N15/52 , A23L33/135 , A61K38/00 , C12N9/1048 , C12P19/04
摘要： The invention provides a gene encoding a protein selected from among the following proteins (a) to (c): (a) a protein having any of the amino acid sequences of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, and 108; (b) a protein which has an amino acid sequence equivalent to any of the amino acid sequences of (a), except that one to several amino acid residues are deleted, substituted, or added, and which exhibits cytokine production regulatory activity; and (c) a protein which has an amino acid sequence having 90% or higher identity to any of the amino acid sequences of (a), and which exhibits cytokine production regulatory activity, as well as a gene useful for regulating cytokine production and use of the gene.
摘要翻译：本发明提供了编码选自以下蛋白质（a）〜（c）的蛋白质的基因：（a）具有SEQ ID NO：2,4,6,8,10,12的任意氨基酸序列的蛋白质，14，16，82，84，86，88，90，92，94，96，98，100，102，104，106及108; （b）具有与（a）的任何氨基酸序列相同的氨基酸序列的蛋白质，除了一至数个氨基酸残基被缺失，取代或添加，并且其表现出细胞因子产生调节活性; 和（c）具有与（a）的任何氨基酸序列具有90％或更高同一性并且表现出细胞因子产生调节活性的氨基酸序列的蛋白质，以及可用于调节细胞因子产生和使用的基因的基因。

7. 发明申请

US20120021418A1 GENE THAT IMPARTS OXYGEN RESISTANCE AND APPLICATION THEREOF 审中-公开
标题翻译：这意味着耐氧性和应用的基因
公开(公告)号：US20120021418A1
公开(公告)日：2012-01-26
申请号：US12995296
申请日：2009-06-12
申请人： Masaki Serata , Tomoyuki Sako
发明人： Masaki Serata , Tomoyuki Sako
IPC分类号： C12Q1/68 , C12N15/74 , C12N1/21 , A23L2/52 , C07H21/04 , C40B40/08 , A23L1/29 , C12N15/53 , C12N15/63
CPC分类号： C12Y118/01002 , A23L33/135 , C12N9/0051 , C12N9/0095 , C12Y108/01009
摘要： To provide a gene useful for imparting oxygen resistance to a microorganism and use of the gene.The oxygen-resistance-imparting gene encoding a protein selected from among the following proteins (a) to (c): (a) a protein having the amino acid sequence of SEQ ID NO: 2 or 6; (b) a protein which has an amino acid sequence equivalent to the amino acid sequence of (a), except that one to several amino acid residues are deleted, substituted, or added, and which exhibits oxygen-resistance-imparting activity; and (c) a protein which has an amino acid sequence having an identity of 85% or higher to the amino acid sequence of (a), and which exhibits oxygen-resistance-imparting activity.
摘要翻译：提供可用于赋予微生物耐氧性和使用该基因的基因。编码选自以下蛋白质（a）〜（c）的蛋白质的耐氧性赋予基因：（a）具有SEQ ID NO：2或6的氨基酸序列的蛋白质; （b）具有与（a）的氨基酸序列相当的氨基酸序列的蛋白质，除了一至数个氨基酸残基被缺失，取代或添加，并且具有耐氧性赋予活性; 和（c）具有与（a）的氨基酸序列同一性为85％以上的氨基酸序列的蛋白质，其具有耐氧性赋予活性。

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