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1. 发明授权

US07446188B2 Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof 失效
标题翻译：植物环丙烷脂肪酸合成酶基因，蛋白质及其用途
公开(公告)号：US07446188B2
公开(公告)日：2008-11-04
申请号：US10499246
申请日：2002-12-20
申请人： Xiaoming Bao , John B. Ohlrogge , Michael R. Pollard
发明人： Xiaoming Bao , John B. Ohlrogge , Michael R. Pollard
IPC分类号： C07H21/04 , A61K36/00
CPC分类号： C12N9/1007 , C12N15/8247
摘要： The present invention provides cyclopropane fatty acid synthase genes and proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also provides methods of using cyclopropane fatty acid synthase genes and proteins, including in their expression in transgenic organisms and in the production of cyclopropane fatty acids in plant oils, and in particular seed oils.
摘要翻译：本发明提供了环丙烷脂肪酸合酶基因和蛋白质及其使用方法。本发明包括合成酶的天然和重组野生型形式，以及突变体和变体形式，其中一些相对于野生型合酶具有改变的特征。本发明还提供了使用环丙烷脂肪酸合酶基因和蛋白质的方法，包括在转基因生物体中的表达和在植物油中特别是种子油中的环丙烷脂肪酸的生产。

2. 发明授权

US07932433B2 Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof 有权
标题翻译：植物环丙烷脂肪酸合成酶基因，蛋白质及其用途
公开(公告)号：US07932433B2
公开(公告)日：2011-04-26
申请号：US12246731
申请日：2008-10-07
申请人： Xiaoming Bao , John B. Ohlrogge , Michael R. Pollard
发明人： Xiaoming Bao , John B. Ohlrogge , Michael R. Pollard
IPC分类号： C12N15/82 , C07H21/04
CPC分类号： C12Q1/6888 , A01K2227/40 , C07K14/705 , C12Q2600/124 , C12Q2600/156
摘要： Cyclopropane fatty acid synthase genes and polypeptides are described. Plants are transformed with such genes to produce such polypeptides.
摘要翻译：描述了环丙烷脂肪酸合成酶基因和多肽。用这些基因转化植物以产生这样的多肽。

3. 发明授权

US09006518B2 F-box protein targeted plant oil production 有权
标题翻译： F-box蛋白靶向植物油生产
公开(公告)号：US09006518B2
公开(公告)日：2015-04-14
申请号：US13039018
申请日：2011-03-02
申请人： John B. Ohlrogge , Sari A. Ruuska , Yonghua Li
发明人： John B. Ohlrogge , Sari A. Ruuska , Yonghua Li
IPC分类号： A01H5/10 , C12N15/82 , C07H21/04 , C07K14/415
CPC分类号： C12N15/8247 , C07K14/415
摘要： Disclosed are genetic constructs, transgenic plant cells and transgenic plants, as well as associated methods, for increasing oil production in a plant using F-box gene sequences.
摘要翻译：公开了遗传构建体，转基因植物细胞和转基因植物以及相关方法，用于使用F盒基因序列增加植物中的油产量。

4. 发明授权

US07915480B2 F-box protein targeted plant oil production 有权
标题翻译： F-box蛋白靶向植物油生产
公开(公告)号：US07915480B2
公开(公告)日：2011-03-29
申请号：US12609562
申请日：2009-10-30
申请人： John B. Ohlrogge , Sari A. Ruuska , Yonghua Li
发明人： John B. Ohlrogge , Sari A. Ruuska , Yonghua Li
IPC分类号： C12N15/82 , C12N15/90
CPC分类号： C12N15/8247 , C07K14/415
摘要： Disclosed are genetic constructs, transgenic plant cells and transgenic plants, as well as associated methods, for increasing oil production in a plant using F-box gene sequences.
摘要翻译：公开了遗传构建体，转基因植物细胞和转基因植物以及相关方法，用于使用F盒基因序列增加植物中的油产量。

5. 发明授权

US07405345B2 Plant seed specific promoters 有权
标题翻译：植物种子特异性启动子
公开(公告)号：US07405345B2
公开(公告)日：2008-07-29
申请号：US11488274
申请日：2006-07-18
申请人： John B. Ohlrogge , Christoph Benning , Hongbo Gao , Thomas Arno Alfred Girke , Joseph A. White
发明人： John B. Ohlrogge , Christoph Benning , Hongbo Gao , Thomas Arno Alfred Girke , Joseph A. White
IPC分类号： C12N15/82 , C12N15/90 , C12N5/10 , A01H5/00 , A01H5/10 , C07H21/04
CPC分类号： C07K14/415 , C12N15/8234
摘要： The present invention relates to novel seed specific promoter regions. The present invention further provide methods of producing proteins and other products of interest and methods of controlling expression of nucleic acid sequences of interest using the seed specific promoter regions. The present invention also provides methods of identifying and isolating novel seed specific promoters.
摘要翻译：本发明涉及新种子特异性启动子区。本发明进一步提供使用种子特异性启动子区域产生蛋白质和其它感兴趣的产物的方法和控制感兴趣的核酸序列表达的方法。本发明还提供鉴定和分离新的种子特异性启动子的方法。

6. 发明授权

US07868156B2 Plant seed specific promoters 有权
标题翻译：植物种子特异性启动子
公开(公告)号：US07868156B2
公开(公告)日：2011-01-11
申请号：US12172832
申请日：2008-07-14
申请人： John B. Ohlrogge , Christoph Benning , Hongbo Gao , Thomas Arno Alfred Girke , Joseph A. White
发明人： John B. Ohlrogge , Christoph Benning , Hongbo Gao , Thomas Arno Alfred Girke , Joseph A. White
IPC分类号： C07H21/04 , C12N5/10 , C12N15/82 , A01H5/10 , A01H5/00
CPC分类号： C07K14/415 , C12N15/8234
摘要： The present invention relates to novel seed specific promoter regions. The present invention further provide methods of producing proteins and other products of interest and methods of controlling expression of nucleic acid sequences of interest using the seed specific promoter regions. The present invention also provides methods of identifying and isolating novel seed specific promoters.
摘要翻译：本发明涉及新种子特异性启动子区。本发明进一步提供使用种子特异性启动子区域产生蛋白质和其它感兴趣的产物的方法和控制感兴趣的核酸序列表达的方法。本发明还提供鉴定和分离新的种子特异性启动子的方法。

7. 发明授权

US5962767A Structure and expression of an arabidopsis acetyl-coenzyme A carboxylase gene 失效
标题翻译：拟南芥乙酰辅酶A羧化酶基因的结构和表达
公开(公告)号：US5962767A
公开(公告)日：1999-10-05
申请号：US790519
申请日：1997-01-29
申请人： John B. Ohlrogge , Keith R. Roesler , Basil S. Shorrosh
发明人： John B. Ohlrogge , Keith R. Roesler , Basil S. Shorrosh
IPC分类号： C12N9/00 , C12N15/82 , A01H5/00 , A01H5/10 , C12N5/04
CPC分类号： C12N9/93 , C12N15/8247
摘要： An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10 kb gene encodes a 251 kD cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.
摘要翻译：拟南芥乙酰辅酶A羧化酶（ACCase）基因已被分离并测序。 10 kb基因编码251 kD胞质ACCase同工酶。该基因的核酸序列如SEQ ID No.1所示，并符合GenBank登录号L27074。 cDNA的cDNA序列和推定的氨基酸序列分别列于SEQ ID No.2和3。通过控制本发明的基因的表达，可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。因此，通过在正义或反义方向引入本发明基因的构建体，可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。因此，也可以控制依赖于丙二酰辅酶A的植物和种子中的脂肪酸合成和延伸。

8. 发明授权

US5925805A Methods of increasing oil content of seeds 失效
标题翻译：增加种子油含量的方法
公开(公告)号：US5925805A
公开(公告)日：1999-07-20
申请号：US677010
申请日：1996-07-01
申请人： John B. Ohlrogge , Keith R. Roesler , Basil S. Shorrosh
发明人： John B. Ohlrogge , Keith R. Roesler , Basil S. Shorrosh
IPC分类号： C12N9/00 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8247 , C12N9/93
摘要： An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10-kb gene encodes a 251-kd cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.
摘要翻译：拟南芥乙酰辅酶A羧化酶（ACCase）基因已被分离并测序。 10-kb基因编码251-kd胞质ACCase同工酶。该基因的核酸序列如SEQ ID No.1所示，并符合GenBank登录号L27074。 cDNA的cDNA序列和推定的氨基酸序列分别列于SEQ ID No.2和3。通过控制本发明的基因的表达，可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。因此，通过在正义或反义方向引入本发明基因的构建体，可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。因此，也可以控制依赖于丙二酰辅酶A的植物和种子中的脂肪酸合成和延伸。

9. 发明授权

US07081565B2 Plant seed specific promoters 失效
公开(公告)号：US07081565B2
公开(公告)日：2006-07-25
申请号：US09998059
申请日：2001-11-30
申请人： John B. Ohlrogge , Christoph Benning , Hongbo Gao , Thomas Arno Alfred Girke , Joseph A. White
发明人： John B. Ohlrogge , Christoph Benning , Hongbo Gao , Thomas Arno Alfred Girke , Joseph A. White
IPC分类号： C12N15/82 , C12N5/04 , C07H21/04 , A01H5/00 , A01H5/10
CPC分类号： C07K14/415 , C12N15/8234
摘要： The present invention relates to novel seed specific promoter regions. The present invention further provide methods of producing proteins and other products of interest and methods of controlling expression of nucleic acid sequences of interest using the seed specific promoter regions. The present invention also provides methods of identifying and isolating novel seed specific promoters.

10. 发明授权

US5559220A Gene encoding acetyl-coenzyme A carboxylase 失效
标题翻译：基因编码乙酰辅酶A羧化酶
公开(公告)号：US5559220A
公开(公告)日：1996-09-24
申请号：US418893
申请日：1995-04-07
申请人： Paul G. Roessler , John B. Ohlrogge
发明人： Paul G. Roessler , John B. Ohlrogge
IPC分类号： C12N9/00 , C12N15/29 , C12N15/82 , C12N1/13 , C12N5/04 , C12N9/18 , C12N15/55
CPC分类号： C12N9/93 , C12N15/8241
摘要： A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives thereof which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides.
摘要翻译：编码来自光合生物的乙酰辅酶A羧化酶（ACCase）的DNA，其功能衍生物对某些除草剂具有抑制作用。该基因可以放置在生物体中以增加其脂肪酸含量或使其对某些除草剂具有抗性。

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