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1. 发明授权

US11473140B2 Highly selective omega primer amplification of nucleic acid sequences 有权
公开(公告)号：US11473140B2
公开(公告)日：2022-10-18
申请号：US14554966
申请日：2014-11-26
申请人： Xiaochuan Zhou , Qi Zhu , Nijing Sheng
发明人： Xiaochuan Zhou , Qi Zhu , Nijing Sheng
IPC分类号： C12Q1/6876 , C12Q1/686
摘要： The present invention relates to the field of nucleic acid sequence replication including PCR. Specifically, the present invention relates to methods and compositions for amplifying one or more target sequences from one or more template sequences. In particular, the present invention provides novel primer designs to enhance specificity of PCR reactions. The present invention also provides methods and compositions to perform the selection of specific sequence sections using specific primers and the amplification of all selected sequence sections using a pair of common primers in a single reaction tube.

2. 发明申请

US20220267845A1 Selective Amplfication of Nucleic Acid Sequences 有权
公开(公告)号：US20220267845A1
公开(公告)日：2022-08-25
申请号：US17300756
申请日：2021-10-22
申请人： Xiaochuan Zhou , Qi Zhu , Xiaolin Zhang , Nijing Sheng
发明人： Xiaochuan Zhou , Qi Zhu , Xiaolin Zhang , Nijing Sheng
IPC分类号： C12Q1/6876 , C12Q1/686
摘要： The present invention relates to the field of nucleic acid sequence replication including PCR. Specifically, the present invention relates to methods and compositions for amplifying one or more target sequences from one or more template sequences. In particular, the present invention provides novel primer designs to enhance specificity of PCR reactions. The present invention also provides methods and compositions to perform the selection of specific sequence sections using specific primers and the amplification of all selected sequence sections using a pair of common primers in a single reaction tube.

3. 发明授权

US07838466B2 Device for chemical and biochemical reactions using photo-generated reagents 有权
标题翻译：使用光产生试剂进行化学和生化反应的装置
公开(公告)号：US07838466B2
公开(公告)日：2010-11-23
申请号：US10157442
申请日：2002-05-29
申请人： Xiaolian Gao , Xiaochuan Zhou , Erdogan Gulari
发明人： Xiaolian Gao , Xiaochuan Zhou , Erdogan Gulari
IPC分类号： C40B50/00
CPC分类号： C07K1/047 , B01J19/0046 , B01J2219/00434 , B01J2219/00439 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B82Y30/00 , C07B2200/11 , C07H21/00 , C07K1/045 , C40B40/06 , C40B40/10 , C40B40/12 , C40B50/14 , C40B60/14 , G03F7/00
摘要： This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for selectively converting photogenerated reagent precursors to photogenerated reagents comprising a substrate comprising at least one solid surface containing a plurality of isolated reaction sites; and an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, wherein the irradiation pattern selectively irradiates a plurality of reaction sites without use of a photolithographic mask. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.
摘要翻译：本发明提供了使用原位产生的光产物作为试剂或共试剂在溶液中进行化学和生物化学反应的方法和装置。特别地，本发明提供了用于选择性地将光生试剂前体转化成光生试剂的方法和装置，所述光生试剂包含含有至少一个含有多个分离的反应位点的固体表面的底物; 以及可操作地连接到所述基板的光学系统，所述光学系统包括光源和计算机控制的空间光学调制器以形成照射图案，其中所述照射图案选择性地照射多个反应部位而不使用光刻掩模。本发明的方法和装置具有在固体表面上并行合成分子序列阵列的应用。

4. 发明申请

US20100112558A1 Probe Bead Synthesis and Use 审中-公开
标题翻译：探针珠合成与使用
公开(公告)号：US20100112558A1
公开(公告)日：2010-05-06
申请号：US12264242
申请日：2008-11-03
申请人： Xiaolian Gao , Xiaochuan Zhou
发明人： Xiaolian Gao , Xiaochuan Zhou
IPC分类号： C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6834 , C12Q2563/155 , C12Q2563/131 , C12Q2525/131
摘要： The present invention relates to the field of methods and devices of miniaturized synthesis. More specifically, the present invention relates to the parallel synthesis of large number of different types of molecules and oligomers, such as oligonucleotides (oligos), peptides, lipids, carbohydrates, small ligand molecules, and other organic and inorganic molecules as probes for multiplexing assays. The probes may be synthesized from and/or attached to nanobeads to microbeads. The present invention provides for assays of multiplexing large scale biology, such as analysis of genomic DNAs and RNAs and proteomic proteins or peptides performed simultaneously on the synthetic beads.
摘要翻译：本发明涉及小型化合成方法和装置的领域。更具体地说，本发明涉及大量不同类型的分子和寡聚体的并行合成，例如寡核苷酸（寡核苷酸），肽，脂质，碳水化合物，小配体分子和其它有机和无机分子作为多重测定的探针。探针可以从和/或连接到纳米珠与微珠上合成。本发明提供多重大规模生物学的测定，例如在合成珠上同时进行的基因组DNA和RNA的分析和蛋白质组蛋白质或肽。

5. 发明申请

US20100047875A1 Making nucleic acid sequences in parallel and use 有权
标题翻译：使核酸序列并行并使用
公开(公告)号：US20100047875A1
公开(公告)日：2010-02-25
申请号：US12384661
申请日：2009-04-07
申请人： Xiaioian Gao , Xiaochuan Zhou , Xiaolin Zheng , Niling Sheng , Qi Zhu
发明人： Xiaioian Gao , Xiaochuan Zhou , Xiaolin Zheng , Niling Sheng , Qi Zhu
IPC分类号： C12P19/34
CPC分类号： C12P19/34
摘要： The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins.
摘要翻译：本发明一般涉及基因组学，合成生物学和基因工程领域。更具体地说，本发明涉及能够在表面上并行多重连接和扩增用于制备各种生物学应用的核酸组装和用于分析生物学样品例如DNA，RNA和蛋白质的方法。

6. 发明申请

US20070281357A1 FLUIDIC METHODS FOR DEVICES FOR PARALLEL CHEMICAL REACTIONS 审中-公开
标题翻译：用于平行化学反应的装置的流体方法
公开(公告)号：US20070281357A1
公开(公告)日：2007-12-06
申请号：US11733112
申请日：2007-04-09
申请人： Xiaochuan Zhou , Tiecheng Zhou , David Sun
发明人： Xiaochuan Zhou , Tiecheng Zhou , David Sun
IPC分类号： G01N33/00 , B01J19/00 , B23P17/04 , C07K1/00
CPC分类号： B01J19/0046 , B01J19/0093 , B01J2219/00279 , B01J2219/00306 , B01J2219/00313 , B01J2219/00322 , B01J2219/00351 , B01J2219/00495 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/00734 , B01J2219/00783 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/0086 , B01J2219/00936 , B01J2219/00943 , B01L3/5025 , B82Y30/00 , C07K1/045 , C07K1/047 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , Y10T29/49
摘要： Fluidic methods and devices for conducting parallel chemical reactions are disclosed. The methods are based on the use of in situ photogenerated reagents such as photogenerated acids, photogenerated bases, or any other suitable chemical compounds that produce active reagents upon light radiation. The present invention describes devices and methods for performing a large number of parallel chemical reactions without the use of a large number of valves, pumps, and other complicated fluidic components. The present invention provides microfluidic devices that contain a plurality of microscopic vessels for carrying out discrete chemical reactions. Other applications may include the preparation of microarrays of DNA and RNA oligonucleotides, peptides, oligosacchrides, phospholipids and other biopolymers on a substrate surface for assessing gene sequence information, screening for biological and chemical activities, identifying intermolecular complex formations, and determining structural features of molecular complexes.
摘要翻译：公开了用于进行平行化学反应的流体方法和装置。这些方法基于使用原位光生试剂，例如光生酸，光生碱或在光辐射时产生活性试剂的任何其它合适的化合物。本发明描述了在不使用大量阀，泵和其它复杂流体组分的情况下进行大量并行化学反应的装置和方法。本发明提供了包含用于进行离散化学反应的多个微观容器的微流体装置。其他应用可能包括在底物表面上制备DNA和RNA寡核苷酸，肽，寡糖，磷脂和其他生物聚合物的微阵列，用于评估基因序列信息，筛选生物和化学活性，鉴定分子间复合物结构，以及确定分子的结构特征复合物。

7. 发明授权

US08883411B2 Making nucleic acid sequences in parallel and use 有权
标题翻译：使核酸序列并行并使用
公开(公告)号：US08883411B2
公开(公告)日：2014-11-11
申请号：US12384661
申请日：2009-04-07
申请人： Xiaioian Gao , Xiaochuan Zhou , Xiaolin Zhang , Nijing Sheng , Qi Zhu
发明人： Xiaioian Gao , Xiaochuan Zhou , Xiaolin Zhang , Nijing Sheng , Qi Zhu
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34
摘要： The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins.
摘要翻译：本发明一般涉及基因组学，合成生物学和基因工程领域。更具体地说，本发明涉及能够在表面上并行多重连接和扩增用于制备各种生物学应用的核酸组装和用于分析生物学样品例如DNA，RNA和蛋白质的方法。

8. 发明申请

US20130261026A1 LINKERS AND CO-COUPLING AGENTS FOR OPTIMIZATION OF OLIGONUCLEOTIDE SYNTHESIS AND PURIFICATION ON SOLID SUPPORTS 审中-公开
标题翻译：用于优化寡核苷酸合成和固相支持物的连接剂和偶联剂
公开(公告)号：US20130261026A1
公开(公告)日：2013-10-03
申请号：US13914125
申请日：2013-06-10
申请人： Xiaolian Gao , Hua Zhang , Peillin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peillin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
IPC分类号： C07H21/00 , C07K2/00
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
摘要翻译：描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。稳定的接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。

9. 发明申请

US20120071358A1 Fluidic devices and methods for multiplex chemical and biochemical reactions 审中-公开
标题翻译：用于多重化学和生化反应的流体装置和方法
公开(公告)号：US20120071358A1
公开(公告)日：2012-03-22
申请号：US13134391
申请日：2011-06-06
申请人： Xiaochuan Zhou , Xiaolian Gao
发明人： Xiaochuan Zhou , Xiaolian Gao
IPC分类号： C40B50/06 , C12P19/34
CPC分类号： C12Q1/6844 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/0605 , B01L2300/0816 , B01L2300/0864 , B01L2300/0874 , B01L2300/0883 , B01L2400/0406 , B01L2400/0487 , B01L2400/0688 , C12Q2537/143 , C12Q2565/501
摘要： The present invention describes microfluidic devices that provide novel fluidic structures to facilitate the separation of fluids into isolated, pico-liter sized compartments for performing multiplexing chemical and biological reactions. Applications of the novel devices including biomolecule synthesis, polynucleotide amplification, and binding assays are also disclosed.
摘要翻译：本发明描述了微流体装置，其提供新的流体结构以便于将流体分离成用于进行多重化学和生物反应的分离的，微微升的隔间。还公开了包括生物分子合成，多核苷酸扩增和结合测定的新型设备的应用。

10. 发明申请

US20110170075A1 DEVICE FOR CHEMICAL AND BIOCHEMICAL REACTIONS USING PHOTO-GENERATED REAGENTS 审中-公开
标题翻译：使用照片生成试剂进行化学和生化反应的装置
公开(公告)号：US20110170075A1
公开(公告)日：2011-07-14
申请号：US12949988
申请日：2010-11-19
申请人： Xiaolian Gao , Xiaochuan Zhou , Erdogan Gulari
发明人： Xiaolian Gao , Xiaochuan Zhou , Erdogan Gulari
IPC分类号： G03B27/00
CPC分类号： C07K1/047 , B01J19/0046 , B01J2219/00434 , B01J2219/00439 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B82Y30/00 , C07B2200/11 , C07H21/00 , C07K1/045 , C40B40/06 , C40B40/10 , C40B40/12 , C40B50/14 , C40B60/14 , G03F7/00
摘要： This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. Specifically, the method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.
摘要翻译：本发明提供了使用原位产生的光产物作为试剂或共试剂在溶液中进行化学和生物化学反应的方法和装置。具体地说，本发明的方法和装置具有在固体表面上并行合成分子序列阵列的应用。

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