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1. 发明申请

US20120046200A1 Screening Methods Involving the Detection of Short-Lived Proteins 有权
标题翻译：涉及短寿命蛋白检测的筛选方法
公开(公告)号：US20120046200A1
公开(公告)日：2012-02-23
申请号：US13288772
申请日：2011-11-03
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C40B30/00 , C40B30/10
CPC分类号： C40B30/04 , C12Q1/6897 , G01N33/6845
摘要： A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.
摘要翻译：提供了用于筛选影响蛋白质降解速率的试剂的方法，所述方法包括：获取细胞文库，表达包含报道蛋白的融合蛋白的细胞和由源自下列样品的cDNA文库的序列编码的蛋白质：细胞，来自cDNA文库的序列在细胞文库内变化; 使细胞库与可能影响蛋白质降解速率的多种试剂接触; 对于每个试剂，基于细胞是否具有与文库中的其他细胞不同的报道信号强度来选择表达短寿命蛋白质的文库中的细胞，所述差异表示选择的表达比所表达的融合蛋白更短的生物融合蛋白的细胞由图书馆内的其他细胞; 并表征由所选细胞表达的每种试剂的融合蛋白。

2. 发明申请

US20070161029A1 High throughput profiling of methylation status of promoter regions of genes 审中-公开
标题翻译：基因启动子区甲基化状态的高通量分析
公开(公告)号：US20070161029A1
公开(公告)日：2007-07-12
申请号：US11634359
申请日：2006-12-04
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6834 , C12Q2537/164 , C12Q2522/101
摘要： Rapid, sensitive, reproducible high-throughput methods for detecting methylation patterns in samples of nucleic acid, especially in the promoter region of genes which are enriched with CpG islands, are provided. The methods include isolating complexes of methylated DNA and methylation binding protein, optionally amplifying the isolated methylated DNA, and detecting the methylated DNA or its amplification products in a multiplex and robust manner. By using the inventive methodology, methylated and unmethylated sequences present in the original sample of nucleic acid can be distinguished. By profiling and comparing the methylation status of genes in different samples, one can utilize the information for diagnosis and treatment of diseases or conditions associated with aberrant DNA hypermethylation or hypomethylation.
摘要翻译：提供了用于检测核酸样品中甲基化模式的快速，灵敏，可重复的高通量方法，特别是在富含CpG岛的基因的启动子区中。方法包括分离甲基化DNA和甲基化结合蛋白的复合物，任选地扩增分离的甲基化DNA，并以多重和鲁棒的方式检测甲基化DNA或其扩增产物。通过使用本发明的方法，可以区分存在于原核酸样品中的甲基化和非甲基化序列。通过分析和比较不同样品中基因的甲基化状态，可以利用该信息进行与异常DNA甲基化或低甲基化相关的疾病或病症的诊断和治疗。

3. 发明申请

US20090181390A1 HIGH THROUGHPUT DETECTION OF MICRORNAS AND USE FOR DISEASE DIAGNOSIS 审中-公开
标题翻译： MICRORNAS的高通量检测和用于疾病诊断的用途
公开(公告)号：US20090181390A1
公开(公告)日：2009-07-16
申请号：US12263152
申请日：2008-10-31
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2525/207 , C12Q2525/313 , C12Q2561/125 , C12Q2525/161
摘要： Methods, compositions and kits are provided for high throughput detection of micro RNAs (miRNA), especially for sensitive and specific detection of miRNA that are in low abundance and closely related to each other. In particular, an assembly of designed oligonucleotide probes with unique tag sequences is used to achieve these purposes via high throughput microarrays, optionally in conjunction with branched-DNA based array detection. The assays can be used for diagnosis, prognosis or monitoring of diseases or disorders such as cancer, for pharmacogenomic studies of patient stratification and drug responses, for discovery of therapeutic targets, or for forensic analysis.
摘要翻译：提供方法，组合物和试剂盒用于微量RNA（miRNA）的高通量检测，特别是对于低丰度且彼此密切相关的miRNA的敏感和特异性检测。特别地，使用具有独特标签序列的设计的寡核苷酸探针的组合来通过高通量微阵列实现这些目的，任选地与基于分支DNA的阵列检测结合。该测定可用于疾病或病症如癌症的诊断，预后或监测，患者分层和药物反应的药物基因组研究，用于发现治疗靶点或进行法医分析。

4. 发明授权

US07056665B2 Screening methods involving the detection of short-lived proteins 有权
公开(公告)号：US07056665B2
公开(公告)日：2006-06-06
申请号：US10053516
申请日：2002-01-16
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： G01N33/53 , C07H21/04 , C12P19/34
CPC分类号： C40B30/04 , C12Q1/6897 , G01N33/6845
摘要： A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.

5. 发明授权

US08076083B2 Screening methods involving the detection of short-lived proteins 有权
标题翻译：涉及检测短寿命蛋白质的筛选方法
公开(公告)号：US08076083B2
公开(公告)日：2011-12-13
申请号：US12804733
申请日：2010-07-27
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C40B30/04 , C12Q1/6897 , G01N33/6845
摘要： A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.
摘要翻译：提供了用于筛选影响蛋白质降解速率的试剂的方法，所述方法包括：获取细胞文库，表达包含报道蛋白的融合蛋白的细胞和由源自下列样品的cDNA文库的序列编码的蛋白质：细胞，来自cDNA文库的序列在细胞文库内变化; 使细胞库与可能影响蛋白质降解速率的多种试剂接触; 对于每个试剂，基于细胞是否具有与文库中的其他细胞不同的报道信号强度来选择表达短寿命蛋白质的文库中的细胞，所述差异表示选择的表达比所表达的融合蛋白更短的生物融合蛋白的细胞由图书馆内的其他细胞; 并表征由所选细胞表达的每种试剂的融合蛋白。

6. 发明申请

US20090181389A1 QUANTITATIVE MEASUREMENT OF NUCLEIC ACID VIA LIGATION-BASED LINEAR AMPLIFICATION 审中-公开
标题翻译：通过基于LIGATION的线性放大对核酸的定量测量
公开(公告)号：US20090181389A1
公开(公告)日：2009-07-16
申请号：US12263099
申请日：2008-10-31
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6851 , C12Q2561/125 , C12Q2525/143 , C12Q2537/143 , C12Q2531/101 , C12Q2521/119 , C12Q2525/161 , C12Q2521/501
摘要： Methods, compositions and kits are provided for sensitive and quantitative detection of nucleic acid, especially for the determination of the presence and/or amount of a target nucleic acid with mutations or single nucleotide polymorphism (SNP). In particular, assays are provided for amplifying a target nucleic acid via ligation of designed oligonucleotide probes and linear amplification by using an RNA polymerase, such as T7 polymerase. The assays can be used for diagnosis, prognosis or monitoring of diseases or disorders, for pharmacogenomic studies of patient stratification and drug responses, for discovery of therapeutic targets, or for forensic analysis.
摘要翻译：提供方法，组合物和试剂盒用于敏感和定量检测核酸，特别是用于确定具有突变或单核苷酸多态性（SNP）的靶核酸的存在和/或量的量。特别地，提供了通过设计的寡核苷酸探针的连接扩增靶核酸并通过使用RNA聚合酶如T7聚合酶进行线性扩增的测定。该测定可用于疾病或病症的诊断，预后或监测，用于患者分层和药物反应的药物基因组学研究，用于发现治疗靶点或用于法医分析。

7. 发明申请

US20060121517A1 Screening methods involving the detection of short-lived proteins 有权
公开(公告)号：US20060121517A1
公开(公告)日：2006-06-08
申请号：US11303037
申请日：2005-12-14
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C40B40/08 , C12Q1/68 , C12N15/87
CPC分类号： C40B30/04 , C12Q1/6897 , G01N33/6845
摘要： A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.

8. 发明授权

US08460876B2 Screening methods involving the detection of short-lived proteins 有权
公开(公告)号：US08460876B2
公开(公告)日：2013-06-11
申请号：US13288772
申请日：2011-11-03
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C40B30/04 , C12Q1/6897 , G01N33/6845
摘要： A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.

9. 发明申请

US20100304394A1 Screening methods involving the detection of short-lived proteins 有权
公开(公告)号：US20100304394A1
公开(公告)日：2010-12-02
申请号：US12804733
申请日：2010-07-27
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C12Q1/68
CPC分类号： C40B30/04 , C12Q1/6897 , G01N33/6845
摘要： A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.

10. 发明授权

US07790378B2 Screening methods involving the detection of short-lived proteins 有权
标题翻译：涉及检测短寿命蛋白质的筛选方法
公开(公告)号：US07790378B2
公开(公告)日：2010-09-07
申请号：US11303037
申请日：2005-12-14
申请人： Xianqiang Li , Xin Jiang
发明人： Xianqiang Li , Xin Jiang
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C40B30/04 , C12Q1/6897 , G01N33/6845
摘要： A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent.
摘要翻译：提供了用于筛选影响蛋白质降解速率的试剂的方法，所述方法包括：获取细胞文库，表达包含报道蛋白的融合蛋白的细胞和由源自下列样品的cDNA文库的序列编码的蛋白质：细胞，来自cDNA文库的序列在细胞文库内变化; 使细胞库与可能影响蛋白质降解速率的多种试剂接触; 对于每个试剂，基于细胞是否具有与文库中的其他细胞不同的报道信号强度来选择表达短寿命蛋白质的文库中的细胞，所述差异表示选择的表达比所表达的融合蛋白更短的生物融合蛋白的细胞由图书馆内的其他细胞; 并表征由所选细胞表达的每种试剂的融合蛋白。

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