专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07771970B2 Process for preparation of polypeptides of interest from fusion polypeptides 有权
标题翻译：从融合多肽制备目的多肽的方法
公开(公告)号：US07771970B2
公开(公告)日：2010-08-10
申请号：US10484478
申请日：2002-07-26
申请人： Woo-Jong Lee , Heung-Bok Park , Tae-Hoon Cho , Jeong-Min Kim , Yeon-Sung Park
发明人： Woo-Jong Lee , Heung-Bok Park , Tae-Hoon Cho , Jeong-Min Kim , Yeon-Sung Park
IPC分类号： C12P21/00
CPC分类号： C07K14/00 , C07K19/00 , C07K2319/00
摘要： The present invention relates to a process of separation a protein of interest comprising expressing the protein of interest fused with a fusion partner, selectively adsorbing on matrix, and performing efficient cleavage reaction on the fusion protein adsorbed on matrix or separated from the matrix, and recovering the protein of interest. Also, the present invention provides a fusion protein comprising a protein of interest and a fusion partner, wherein the fusion partner comprises an amino acid sequence which can be cleaved by ubiquitin cleavage enzyme at its C-terminus, and which has a difference in isoelectric point of 1 or more from the protein of interest. According to the present invention, the proteins of interest can be purified in high yield and purity. In addition, complicated separating processes required in the production of recombinant proteins can be eliminated so as to cut down the production cost.
摘要翻译：本发明涉及分离感兴趣的蛋白质的方法，包括表达与融合配偶体融合的目的蛋白质，选择性吸附于基质上，并对吸附在基质上或与基质分离的融合蛋白进行有效的切割反应，并回收感兴趣的蛋白质此外，本发明提供了包含目标蛋白质和融合配偶体的融合蛋白质，其中融合配偶体包含可在其C末端被泛素切割酶切割的氨基酸序列，并且其等电点从感兴趣的蛋白质1或更多。根据本发明，可以高产率和纯度纯化目的蛋白质。此外，可以消除生产重组蛋白质所需的复杂分离过程，从而降低生产成本。

2. 发明申请

US20050124790A1 Process for preparation of polypeptides of interest from fusion polypeptides 有权
标题翻译：从融合多肽制备目的多肽的方法
公开(公告)号：US20050124790A1
公开(公告)日：2005-06-09
申请号：US10484478
申请日：2002-07-26
申请人： Woo-Jong Lee , Heung-Bok Park , Tae-Hoon Cho
发明人： Woo-Jong Lee , Heung-Bok Park , Tae-Hoon Cho
IPC分类号： C12N15/09 , C07K1/14 , C07K14/00 , C07K19/00 , C12P21/02 , C07K14/47 , C07H21/04 , C12P21/04
CPC分类号： C07K14/00 , C07K19/00 , C07K2319/00
摘要： The present invention relates to a process of separation a protein of interest comprising expressing the protein of interest fused with a fusion partner, selectively adsorbing on matrix, and performing efficient cleavage reaction on the fusion protein adsorbed on matrix or separated from the matrix, and recovering the protein of interest. Also, the present invention provides a fusion protein comprising a protein of interest and a fusion partner, wherein the fusion partner comprises an amino acid sequence which can be cleaved by ubiquitin cleavage enzyme at its C-terminus, and hwich has a difference in isoelectric point of 1 or more from the protein of interest. According to the present invention, the proteins of interest can be purified in high yield and purity. In addition, complicated separating processes required in the production of recombinant proteins can be eliminated so as to cut down the production cost.
摘要翻译：本发明涉及分离感兴趣的蛋白质的方法，包括表达与融合配偶体融合的目的蛋白质，选择性吸附于基质上，并对吸附在基质上或与基质分离的融合蛋白进行有效的切割反应，并回收感兴趣的蛋白质此外，本发明提供了包含目的蛋白质和融合配偶体的融合蛋白，其中融合配偶体包含可在其C末端被泛素裂解酶切割的氨基酸序列，并且hwich在等电点上具有差异从感兴趣的蛋白质1或更多。根据本发明，可以高产率和纯度纯化目的蛋白质。此外，可以消除生产重组蛋白质所需的复杂分离过程，从而降低生产成本。

3. 发明申请

US20060292662A1 GALLERIA MELLONELLA DERIVED COMPOSITION FOR DETECTING PEPTIDOGLYCAN, A METHOD FOR USE THEREOF, AND A DIAGNOSTIC KIT CONTAINING THE SAME 失效
标题翻译：用于检测肽酶的GALLERIA MELLONELLA衍生的组合物，其使用方法和包含其的诊断试剂盒
公开(公告)号：US20060292662A1
公开(公告)日：2006-12-28
申请号：US11460406
申请日：2006-07-27
申请人： Bu-Soo Park , Chang-Hun Joo , Moon-Suk Kim , Seung-Hwan Song , Jong-Won Yoon , Yeon-Sung Park , Hong-Rak Kim , Joong-Hyuck Auh , Tae-Hoon Cho , Bok-Luel Lee , Ji-Won Park , Jeong-Mi Yeo , Hyun-Sic Kim
发明人： Bu-Soo Park , Chang-Hun Joo , Moon-Suk Kim , Seung-Hwan Song , Jong-Won Yoon , Yeon-Sung Park , Hong-Rak Kim , Joong-Hyuck Auh , Tae-Hoon Cho , Bok-Luel Lee , Ji-Won Park , Jeong-Mi Yeo , Hyun-Sic Kim
IPC分类号： C12Q1/30
CPC分类号： G01N33/56911 , C12Q1/04 , C12Q1/26 , G01N2333/43552
摘要： The present invention relates to a composition for selectively detecting an extremely small amount of peptidoglycan in sample, a preparation method of the composition, and a detection kit for peptidoglycan. It is possible to quantify a small amount of peptidoglycan contained in human blood, tissue, body fluid, water or food, and to diagnose an infection of microorganism with peptidoglycan as a component of cell wall using the composition and the detection kit. In addition, the composition can be applied for a diagnosis reagent of detecting an infection of Gram-positive bacteria in animal or human being in advance, and thus, can be used for the prevention or treatment of food poisonings and bacterial sepsis.
摘要翻译：本发明涉及用于选择性地检测样品中极少量的肽聚糖的组合物，该组合物的制备方法和用于肽聚糖的检测试剂盒。使用组合物和检测试剂盒，可以量化少量的人血液，组织，体液，水或食物中的肽聚糖，并利用肽聚糖作为细胞壁的组分来诊断微生物的感染。此外，该组合物可以预先用于检测动物或人类中革兰氏阳性菌感染的诊断试剂，因此可用于预防或治疗食物中毒和细菌性败血症。

4. 发明授权

US07507553B2 Galleria mellonella derived composition for detecting peptidoglycan, a method for use thereof, and a diagnostic kit containing the same 失效
标题翻译：用于检测肽聚糖的环孢霉烯衍生组合物，其使用方法和含有该肽的诊断试剂盒
公开(公告)号：US07507553B2
公开(公告)日：2009-03-24
申请号：US11460406
申请日：2006-07-27
申请人： Bu-Soo Park , Bok-Luel Lee , Ji-Won Park , Jeong-Mi Yeo , Hyun-Sic Kim , Chang-Hun Joo , Moon-Suk Kim , Seung-Hwan Song , Jong-Won Yoon , Yeon-Sung Park , Hong-Lak Kim , Joong-Hyuck Auh , Tae-Hoon Cho
发明人： Bu-Soo Park , Bok-Luel Lee , Ji-Won Park , Jeong-Mi Yeo , Hyun-Sic Kim , Chang-Hun Joo , Moon-Suk Kim , Seung-Hwan Song , Jong-Won Yoon , Yeon-Sung Park , Hong-Lak Kim , Joong-Hyuck Auh , Tae-Hoon Cho
IPC分类号： C12Q1/26
CPC分类号： G01N33/56911 , C12Q1/04 , C12Q1/26 , G01N2333/43552
摘要： The present invention relates to a composition for selectively detecting an extremely small amount of peptidoglycan in sample, a preparation method of the composition, and a detection kit for peptidoglycan. It is possible to quantify a small amount of peptidoglycan contained in human blood, tissue, body fluid, water or food, and to diagnose an infection of microorganism with peptidoglycan as a component of cell wall using the composition and the detection kit. In addition, the composition can be applied for a diagnosis reagent of detecting an infection of Gram-positive bacteria in animal or human being in advance, and thus, can be used for the prevention or treatment of food poisonings and bacterial sepsis.
摘要翻译：本发明涉及用于选择性地检测样品中极少量的肽聚糖的组合物，该组合物的制备方法和用于肽聚糖的检测试剂盒。使用组合物和检测试剂盒，可以量化少量的人血液，组织，体液，水或食物中的肽聚糖，并利用肽聚糖作为细胞壁的组分来诊断微生物的感染。此外，该组合物可以预先用于检测动物或人类中革兰氏阳性菌感染的诊断试剂，因此可用于预防或治疗食物中毒和细菌性败血症。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式