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1. 发明授权

US09415353B2 Ultrafiltration membranes and methods of making and use of ultrafiltration membranes 有权
标题翻译：超滤膜和制造和使用超滤膜的方法
公开(公告)号：US09415353B2
公开(公告)日：2016-08-16
申请号：US12658980
申请日：2010-02-18
申请人： Wilson Moya , Mikhail Kozlov
发明人： Wilson Moya , Mikhail Kozlov
IPC分类号： B01D71/68 , B01D67/00 , A61L2/00 , B01D69/02 , B01D69/10
CPC分类号： B01D71/68 , A61L2/0017 , B01D67/0083 , B01D67/0088 , B01D67/0093 , B01D69/02 , B01D69/10 , B01D2323/02 , B01D2325/20 , B01D2325/36
摘要： An inherently hydrophobic polymer membrane substrate having its surface rendered hydrophilic with a hydroxyalkyl cellulose and having a throughput greater than about 1500 L/m2 is provided. A process for making the membrane also is provided which includes a step of autoclaving in boiling water and/or steam or submerging in boiling water. The membrane is useful for removing virus from a protein solution.
摘要翻译：本发明提供一种具有亲水性的羟烷基纤维素并具有大于约1500L / m 2的通过量的固有疏水性聚合物膜基材。还提供了制造膜的方法，其包括在沸水和/或蒸汽中高压灭菌或浸没在沸水中的步骤。该膜可用于从蛋白质溶液中除去病毒。

2. 发明授权

US08552177B2 Room temperature stable agarose solutions 有权
标题翻译：室温稳定的琼脂糖溶液
公开(公告)号：US08552177B2
公开(公告)日：2013-10-08
申请号：US13221439
申请日：2011-08-30
申请人： Wilson Moya , Neil P. Soice
发明人： Wilson Moya , Neil P. Soice
IPC分类号： C08B37/02 , C08B37/00 , C07H1/00 , C07H3/00
CPC分类号： C08J3/09 , C08J2305/00 , C08L5/02 , C08L5/12 , D06M11/13 , D06M11/155 , D06M11/38 , D06M15/03 , D06M23/10 , Y10T428/24149 , Y10T428/249953 , Y10T442/20
摘要： A method for making functionalized porous crosslinked polysaccharide gel coated structures used as liquid chromatography media is provided. The method includes impregnating a porous substrate with a room temperature stable aqueous polysaccharide solution containing water, 0.1% to 20% of a polysaccharide, 18% to 54% of a gel-inhibiting agent to prevent the gel from re-gelling, and 0.001% to 10% of an anionic fluorosurfactant for optimum solution coatability onto the substrate, each concentration is by total weight of the aqueous solution. Next water is evaporated from the coating, followed by exposing the dehydrated coating to a gelling agent thereby forming a porous polysaccharide gel coated substrate. Next the gel coated substrate is exposed to a crosslinking agent forming a porous crosslinked polysaccharide gel coated substrate. Next, the gel coated substrate is functionalized by contacting the porous polysaccharide gel coated substrate with sodium 3-bromopropanesulfonate, thereby attaching sulfopropyl ligands to the gel coated substrate, resulting in a functionalized porous absorptive polysaccharide gel coated chromatography structure. When the functionalized gel coated chromatography structure is used as liquid chromatography media, and a protein (e.g. lysozyme) containing solution contacts the media, the sulfopropyl ligands attached to the gel coated chromatography structure will bond to the protein, thereby removing the protein from the protein containg solution.
摘要翻译：提供了用作液相色谱介质的功能化多孔交联多糖凝胶涂层结构的方法。该方法包括用含有水，0.1％至20％的多糖，18％至54％的凝胶抑制剂的室温稳定的水性多糖溶液浸渍多孔基材以防止凝胶重新凝胶化，并且0.001％至10％的阴离子含氟表面活性剂，用于在基材上获得最佳的溶液涂布性，每种浓度均为水溶液的总重量。接下来的水从涂层中蒸发，然后将脱水涂层暴露于胶凝剂，从而形成多孔多糖凝胶涂覆的基质。接下来，将凝胶涂覆的基底暴露于形成多孔交联多糖凝胶涂覆的基底的交联剂。接下来，通过使多孔多糖凝胶涂覆的基质与3-溴丙磺酸钠接触使凝胶涂覆的底物功能化，从而将磺丙基配体连接到凝胶涂覆的基质上，得到官能化的多孔吸收多糖凝胶涂层色谱结构。当将功能化凝胶涂层色谱结构用作液相色谱介质，并且含有蛋白质（例如溶菌酶）的溶液与培养基接触时，附着在凝胶涂层色谱结构上的磺丙基配体将与蛋白质结合，从而从蛋白质中除去蛋白质包含解决方案

3. 发明申请

US20120193278A1 Media For Membrane Ion Exchange Chromatography Based On Polymeric Primary Amines, Sorption Device Containing That Media, And Chromatography Scheme And Purification Method Using The Same 有权
标题翻译：基于聚合一级胺的膜离子交换色谱介质，含有该介质的吸附装置和色谱方案及使用其的纯化方法
公开(公告)号：US20120193278A1
公开(公告)日：2012-08-02
申请号：US13363397
申请日：2012-02-01
申请人： Mikhail Kozlov , Wilson Moya , Michael W. Phillips , Senthilkumar Ramaswamy , Brian Gagnon
发明人： Mikhail Kozlov , Wilson Moya , Michael W. Phillips , Senthilkumar Ramaswamy , Brian Gagnon
IPC分类号： B01D15/10
CPC分类号： B01J20/28033 , B01D15/1871 , B01D15/362 , B01D15/363 , B01D15/3804 , B01J20/3282 , B01J47/12 , Y10T428/249987
摘要： Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities even at high conductivities and pH.
摘要翻译：介质和装置，例如包括这种介质的阴离子交换剂，其中介质是具有涂覆聚合物如聚烯丙基胺的表面的膜。与基于季铵盐（包括三甲基铵配体）的常规配体相比，所得到的膜提供了更强的蛋白质杂质结合和优于宿主细胞蛋白质从生物样品中的去除。还描述了用于纯化单克隆抗体的色谱方法和方法，其中阴离子交换吸附剂置于亲和柱（例如蛋白A或蛋白G亲和柱）的下游，以及任选的一种或多种抛光装置如阳离子交换柱。阳离子交换池（或不使用阳离子交换剂的亲和柱交换池）的稀释很少或不需要，以降低样品的电导率。即使在高电导率和pH下，吸附剂也能很好地结合宿主细胞蛋白质和其他杂质。

4. 发明申请

US20120121819A1 Media For Membrane Ion Exchange Chromatography Based On Polymeric Primary Amines, Sorption Device Containing That Media, And Chromatography Scheme And Purification Method Using The Same 审中-公开
标题翻译：基于聚合一级胺的膜离子交换色谱介质，含有该介质的吸附装置和色谱方案及使用其的纯化方法
公开(公告)号：US20120121819A1
公开(公告)日：2012-05-17
申请号：US13339503
申请日：2011-12-29
申请人： Mikhail Kozlov , Wilson Moya , Michael W. Phillips , Senthilkumar Ramaswamy , Brian Gagnon
发明人： Mikhail Kozlov , Wilson Moya , Michael W. Phillips , Senthilkumar Ramaswamy , Brian Gagnon
IPC分类号： B05D5/00 , B05D3/06
CPC分类号： B01J20/28033 , B01D15/1871 , B01D15/362 , B01D15/363 , B01D15/3804 , B01J20/3282 , B01J47/12 , Y10T428/249987
摘要： Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (e.g., Protein A or Protein G) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.
摘要翻译：介质和装置，例如包括这种介质的阴离子交换剂，其中介质是具有涂覆聚合物如聚烯丙基胺的表面的膜。与基于季铵盐（包括三甲基铵配体）的常规配体相比，所得到的膜提供了更强的蛋白质杂质结合和优于宿主细胞蛋白质从生物样品中的去除。还描述了用于纯化单克隆抗体的色谱方案和方法，其中阴离子交换吸附剂置于亲和柱（例如蛋白A或蛋白G）和任选的一种或多种抛光装置如阳离子交换柱的下游。阳离子交换池（或不使用阳离子交换剂的亲和柱交换池）的稀释很少或不需要，以降低样品的电导率。即使在高电导率和pH下，吸附剂也能很好地结合生物样品中的宿主细胞蛋白质和其他杂质。

5. 发明授权

US08137561B2 Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same 有权
标题翻译：用于基于聚合伯胺的膜离子交换色谱的介质，含有该介质的吸附装置和使用其的色谱方案和纯化方法
公开(公告)号：US08137561B2
公开(公告)日：2012-03-20
申请号：US13167297
申请日：2011-06-23
申请人： Mikhail Kozlov , Wilson Moya , Michael W. Phillips , Senthilkumar Ramaswamy , Brian Gagnon
发明人： Mikhail Kozlov , Wilson Moya , Michael W. Phillips , Senthilkumar Ramaswamy , Brian Gagnon
IPC分类号： B01D15/08
CPC分类号： B01J20/28033 , B01D15/1871 , B01D15/362 , B01D15/363 , B01D15/3804 , B01J20/3282 , B01J47/12 , Y10T428/249987
摘要： Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.
摘要翻译：介质和装置，例如包括这种介质的阴离子交换剂，其中介质是具有涂覆聚合物如聚烯丙基胺的表面的膜。与基于季铵盐（包括三甲基铵配体）的常规配体相比，所得到的膜提供了更强的蛋白质杂质结合和优于宿主细胞蛋白质从生物样品中的去除。还描述了用于纯化单克隆抗体的色谱方法和方法，其中阴离子交换吸附剂置于亲和柱（例如蛋白A或蛋白G亲和柱）的下游，以及任选的一种或多种抛光装置如阳离子交换柱。阳离子交换池（或不使用阳离子交换剂的亲和柱交换池）的稀释很少或不需要，以降低样品的电导率。即使在高电导率和pH下，吸附剂也能很好地结合生物样品中的宿主细胞蛋白质和其他杂质。

6. 发明授权

US07717273B2 Membrane surface modification by radiation-induced polymerization 失效
标题翻译：通过辐射诱导聚合进行膜表面改性
公开(公告)号：US07717273B2
公开(公告)日：2010-05-18
申请号：US11800821
申请日：2007-05-08
申请人： Mikhail Kozlov , Wilson Moya
发明人： Mikhail Kozlov , Wilson Moya
IPC分类号： B01D71/06 , B01D29/00 , B01D39/00 , B01D67/00
CPC分类号： B01D67/0093 , B01D71/36 , B01D71/82 , B01D2323/02
摘要： The invention provides porous membranes with at least a partially modified surface as well as methods of making and using the same.
摘要翻译：本发明提供具有至少部分改性表面的多孔膜以及制备和使用它们的方法。

7. 发明申请

US20080255027A1 Purification of proteins 有权
标题翻译：蛋白质的纯化
公开(公告)号：US20080255027A1
公开(公告)日：2008-10-16
申请号：US12004319
申请日：2007-12-20
申请人： Wilson Moya , Jad Jaber
发明人： Wilson Moya , Jad Jaber
IPC分类号： A61K38/02 , B01D15/08 , C07K1/32
CPC分类号： C07K1/32 , C07K1/30
摘要： The present invention relates to a selectively soluble polymer capable of binding to a desired biomolecules in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired biomolecule (protein, polypeptide, etc) and remains capable of binding to that biomolecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.
摘要翻译：本发明涉及能够在含有各种生物材料的混合物中结合所需生物分子的选择性可溶性聚合物以及使用这种聚合物从这种混合物中纯化生物分子的方法。聚合物在一定的工艺条件例如pH或温度和/或盐浓度下可溶于混合物中，并且在工艺条件改变时变得不溶解并沉淀出溶液。聚合物能够结合所需的生物分子（蛋白质，多肽等），并且即使在聚合物从溶液中沉淀出来之后仍然能够结合该生物分子。然后可以从流的其余部分过滤出沉淀物，并回收所需的生物分子，例如通过洗脱和进一步处理。

8. 发明申请

US20070256970A1 Porous adsorptive or chromatographic media 有权
标题翻译：多孔吸附或色谱介质
公开(公告)号：US20070256970A1
公开(公告)日：2007-11-08
申请号：US11821059
申请日：2007-06-21
申请人： Anthony DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil Soice , Volkmar Thom , Sarah Yuan
发明人： Anthony DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil Soice , Volkmar Thom , Sarah Yuan
IPC分类号： B01J20/22
CPC分类号： B01D15/20 , B01D15/265 , B01D15/361 , B01J20/26 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/28078 , B01J20/28097 , B01J20/286 , B01J20/3064 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J20/3293 , B01J2220/54 , B01J2220/58 , B01J2220/62
摘要： A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.
摘要翻译：能够吸附过滤具有多孔自支撑基底和一种或多种多孔吸附聚合物涂层的多孔基底，其包含基底孔隙体积的约1至约80％。所得到的底物具有良好的对流和扩散流量和容量。底物可以是交联的，具有一个或多个捕获化学物质连接到其上，并且可用作用于选择性过滤所需物质（包括生物分子例如蛋白质和DNA片段）的色谱介质。

9. 发明授权

US06627291B1 Three dimensional patterned porous structures 有权
标题翻译：三维图案化多孔结构
公开(公告)号：US06627291B1
公开(公告)日：2003-09-30
申请号：US09661104
申请日：2000-09-13
申请人： Phillip Clark , Wilson Moya
发明人： Phillip Clark , Wilson Moya
IPC分类号： B32B310
CPC分类号： B01D67/0083 , B01D61/18 , B01D67/0086 , B01D69/02 , B01J20/28033 , B01L3/5023 , B01L3/5025 , B01L3/5085 , B01L2200/12 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2300/0887 , B01L2400/0406 , Y10T428/24331 , Y10T428/24826 , Y10T428/249987 , Y10T428/249988 , Y10T428/249989 , Y10T428/249992
摘要： The present invention provides new patterned porous substrates and a new method for forming the new patterned porous substrates containing one or more selected patterns which is applicable to all porous structures regardless of their polymeric content, their method of manufacture or their UV absorbing characteristics.
摘要翻译：本发明提供新的图案化多孔基材和用于形成新的图案化多孔基材的新方法，其包含可应用于所有多孔结构的一种或多种选择的图案，而不管其聚合物含量，其制造方法或其UV吸收特性如何。

10. 发明授权

US5629084A Porous composite membrane and process 失效
标题翻译：多孔复合膜及工艺
公开(公告)号：US5629084A
公开(公告)日：1997-05-13
申请号：US545230
申请日：1995-10-19
申请人： Wilson Moya
发明人： Wilson Moya
IPC分类号： B01D67/00 , B01D69/12 , C08J9/00 , C08J9/36 , D06M15/19 , B32B3/26 , B32B5/14
CPC分类号： B01D67/0088 , B01D69/125 , Y10T428/249958 , Y10T428/249978 , Y10T428/249979 , Y10T428/24998 , Y10T428/249981
摘要： A composite porous membrane is provided which comprises a porous membrane substrate having an average pore size between 0.01 and 10 microns formed of a first polymer which is coated over its entire surface with a crosslinked second polymer. The second polymer is dissolved in a solvent with a free radical polymerization initiator in the absence of a crosslinking agent. The second polymer is crosslinked in situ and rendered insoluble by mild heating and/or exposure to ultraviolet light.
摘要翻译：提供了一种复合多孔膜，其包括由第一聚合物形成的平均孔径为0.01至10微米的多孔膜基材，该第一聚合物在其整个表面上用交联的第二聚合物涂覆。第二种聚合物在不存在交联剂的情况下用自由基聚合引发剂溶解在溶剂中。第二聚合物原位交联并通过温和加热和/或暴露于紫外光使其变得不溶。

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