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    • 3. 发明授权
    • In vitro propagation of grape
    • 葡萄的体外繁殖
    • US4714679A
    • 1987-12-22
    • US749306
    • 1985-06-27
    • William R. Krul
    • William R. Krul
    • A01H4/00A01G1/00
    • A01H4/005
    • A process for controlling the development of somatic embryos for either a self-replicative or a vine development mode. Somatic embryogenesis is initiated by placing the embryo in a medium conducive for self-replication. When the embryos have grown to a first stage, they are transferred to a medium with cytokinin activity. They continue to grow until they have grown to a second stage at which time they are transferred to a medium without cytokinin activity until they have grown sufficiently and can be transferred to a non-liquid growth medium.
    • 用于控制体细胞胚发育的过程,用于自我复制或藤蔓发育模式。 通过将胚胎置于有助于自我复制的培养基中来启动体细胞胚发生。 当胚胎生长到第一阶段时,它们被转移到具有细胞分裂素活性的培养基中。 它们继续生长,直到它们生长到第二阶段,此时它们被转移到没有细胞分裂素活性的培养基中,直到它们已经充分生长并且可以转移到非液体生长培养基。