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1. 发明授权

US08517319B2 Apparatus for mounting for multiple lasers 有权
标题翻译：用于安装多个激光器的装置
公开(公告)号：US08517319B2
公开(公告)日：2013-08-27
申请号：US11610120
申请日：2006-12-13
申请人： William C. Hay , Ingo Böhm , Andreas Gorshöfer
发明人： William C. Hay , Ingo Böhm , Andreas Gorshöfer
IPC分类号： F16M11/00 , G02B21/00
CPC分类号： G02B21/0032 , G01J3/10
摘要： An apparatus for mounting multiple lasers, including a mounting plate configured to receive multiple lasers and including a first side and a second side, wherein the first side and the second side are not coplanar; at least six leg portions, each including a support end; wherein at least three of the leg portions project away from the first side of the mounting plate and at least three of the leg portions project away from the second side of the mounting plate; and wherein the support ends of the leg portions projecting away from the first side are configured to support the mounting plate when the first side is facing in a generally downward direction, and the support ends of the leg portions projecting away from the second side are configured to support the mounting plate when the second side is facing in a generally downward direction.
摘要翻译：一种用于安装多个激光器的装置，包括构造成接收多个激光器并且包括第一侧面和第二侧面的安装板，其中第一侧面和第二侧面不是共面的; 至少六个腿部，每个包括支撑端; 其中所述腿部中的至少三个从所述安装板的第一侧突出，并且所述至少三个所述腿部远离所述安装板的第二侧突出; 并且其中，所述腿部从所述第一侧突出的支撑端构造成在所述第一面朝向大致向下的方向时支撑所述安装板，并且所述脚部的从所述第二侧突出的支撑端配置当第二面朝向大致向下的方向时支撑安装板。

2. 发明申请

US20100134793A1 OPTICAL EVALUATION METHOD BY MEANS OF LASER PULSES AND CORRESPONDING APPARATUS 有权
标题翻译：激光脉冲和相关装置的光学评估方法
公开(公告)号：US20100134793A1
公开(公告)日：2010-06-03
申请号：US12626057
申请日：2009-11-25
申请人： Vishnu Vardhan Krishnamachari , William C. Hay , Volker Seyfried , Bernd Widzgowski
发明人： Vishnu Vardhan Krishnamachari , William C. Hay , Volker Seyfried , Bernd Widzgowski
IPC分类号： G01J3/30 , G01J3/44
CPC分类号： G01N21/63
摘要： An optical evaluation method and an apparatus for performing said method are described. First laser pulses of a first type and second laser pulses of a second type that differs from the first type are sent onto a sample to be examined. The sample is hit with first incident light from the two laser pulses in at least one manner of simultaneously, within a very short time lag between the two laser pulses, and a time-correlated manner of the two laser pulses, thereby generating a first optical signal, and hit with second incident light from the two laser pulses, thereby generating a second optical signal. The generated first and second optical signals are detected with at least one detector; and an electronic difference between the first and second optical signals is generated.
摘要翻译：描述了用于执行所述方法的光学评估方法和装置。第一类型的第一激光脉冲和第二类型的与第一类型不同的第二激光脉冲被发送到要检查的样品上。在两个激光脉冲之间的非常短的时间间隔内，以两个激光脉冲的时间相关方式，以至少一种方式同时来自两个激光脉冲的第一入射光和第二激光脉冲的时间相关方式，信号，并用来自两个激光脉冲的第二入射光击中，从而产生第二光信号。产生的第一和第二光信号用至少一个检测器检测; 并且产生第一和第二光信号之间的电子差异。

3. 发明授权

US06806950B2 Apparatus for determining a light power level, microscope, and method for microscopy 有权
标题翻译：用于确定光功率水平的装置，显微镜和用于显微镜的方法
公开(公告)号：US06806950B2
公开(公告)日：2004-10-19
申请号：US10154938
申请日：2002-05-24
申请人： Heinrich Ulrich , Holger Birk , William C. Hay , Holger Nissle
发明人： Heinrich Ulrich , Holger Birk , William C. Hay , Holger Nissle
IPC分类号： G01J142
CPC分类号： G02B27/108 , G02B21/00 , G02B27/144 , G11B7/00
摘要： An apparatus for determining the light power level of a light beam (7), having a beam splitter (1) and a detector (11) associated with the beam splitter (1), is disclosed. The apparatus is characterized in that the beam splitter (1) splits measuring light (23) out of the light beam and conveys it to the detector (11), and that the ratio between the light power level of the light beam (7) and the light power level of the measuring light (23) measured at the detector (11) is constant over time.
摘要翻译：公开了一种用于确定具有与分束器（1）相关联的分束器（1）和检测器（11）的光束（7）的光功率水平的装置。该装置的特征在于，分束器（1）将测量光（23）分离出光束并将其传送到检测器（11），并且光束（7）的光功率级与在检测器（11）处测量的测量光（23）的光功率水平随时间而是恒定的。

4. 发明授权

US08610996B2 Tunable multiple laser pulse scanning microscope and method of operating the same 有权
标题翻译：可调多重激光脉冲扫描显微镜及其操作方法
公开(公告)号：US08610996B2
公开(公告)日：2013-12-17
申请号：US12775395
申请日：2010-05-06
申请人： Vishnu Vardhan Krishnamachari , William C. Hay
发明人： Vishnu Vardhan Krishnamachari , William C. Hay
IPC分类号： G02F1/11
CPC分类号： G02B21/0032 , G02B21/0076
摘要： A tunable multiple laser pulse scanning microscope and a method of operating the same is described, applying two pulsed laser beams with distinct wavelengths incident on a scanning spot of a sample to be imaged simultaneously or at a specific time delay. The microscope comprises at least two pulsed laser light sources emitting laser light of distinct wavelengths, an acousto-optic tunable filter (AOTF) for tuning at least one of the laser pulses, a delay stage provided upstream of the AOTF, and an actuator for moving delay stage depending on the time delay. As a result, the wavelength of at least one type of pulses is tuned, and the delay between at least two pulses of distinct wavelengths is adjusted.
摘要翻译：描述了可调多激光脉冲扫描显微镜及其操作方法，应用两个具有不同波长的脉冲激光束入射到要同时成像的样品的扫描点或者以特定的时间延迟。显微镜包括至少两个发射不同波长的激光的脉冲激光光源，用于调谐至少一个激光脉冲的声光可调谐滤波器（AOTF），设置在AOTF上游的延迟级，以及用于移动的致动器延迟时间取决于时间延迟。结果，调谐至少一种类型的脉冲的波长，并且调整不同波长的至少两个脉冲之间的延迟。

5. 发明授权

US06785302B1 Optical system in the ray path of a confocal fluorescence microscope 有权
标题翻译：光学系统在共焦荧光显微镜的射线路径
公开(公告)号：US06785302B1
公开(公告)日：2004-08-31
申请号：US09554083
申请日：2000-05-12
申请人： Johann Engelhardt , Heinrich Ulrich , William C. Hay
发明人： Johann Engelhardt , Heinrich Ulrich , William C. Hay
IPC分类号： H01S3121
CPC分类号： G01N21/6458 , G02B21/0024 , G02B21/0076 , G02B21/008
摘要： The invention relates to an optical system in the ray path of a confocal fluorescence microscope, comprising at least one laser light source (1, 2), a device positioned in the illuminating/detecting beam (3, 4, 5) for separating the exciting light (8) from the fluorescent light (9), an objective (10) arranged between the device and the object (7), and a detector (11) positioned downstream of the device situated in the detecting beam (5). The aim of the invention is to increase the fluorescence yield of the system while retaining its compact structure. To this end the separating device comprises a mirror (13) which is dimensioned and positioned in the illuminating and/or detecting beam (3, 4, 5) in such a way that for the dark-field illumination of the object (7) it reflects the non-expanded exciting beam arriving from the laser light source (1, 2) into the objective (10) and permits the transit of the fluorescence light (9) arriving from the object (7) in the direction of the detector (11) with full numerical aperture, the fluorescent light beam being reduced by the mirror (13) cross-section active in the detecting beam (5).
摘要翻译：本发明涉及一种共聚焦荧光显微镜的光线路径中的光学系统，包括至少一个激光光源（1,2），位于照明/检测光束（3,4,5）中的装置，用于分离激发光来自荧光灯（9）的光（8），布置在装置和物体（7）之间的物镜（10）和位于位于检测光束（5）中的装置下游的检测器（11）。本发明的目的是提高系统的荧光收率，同时保持其紧凑的结构。为此，分离装置包括反射镜（13），该反射镜的尺寸设置在照明和/或检测光束（3,4,5）中，使得对于物体（7）的暗场照明反射从激光光源（1,2）到达物镜（10）的未扩展的激发光束，并允许从物体（7）到达检测器（11）的方向的荧光（9）的转移）具有全数值孔径，荧光光束被检测光束（5）中的反射镜（13）横截面减小。

6. 发明授权

US06392794B1 Laser scanning microscope 有权
标题翻译：激光扫描显微镜
公开(公告)号：US06392794B1
公开(公告)日：2002-05-21
申请号：US09660655
申请日：2000-09-13
申请人： Johann Engelhardt , Heinrich Ulrich , William C. Hay
发明人： Johann Engelhardt , Heinrich Ulrich , William C. Hay
IPC分类号： G02B2100
CPC分类号： G02B21/0056 , G02B21/0032 , G02B21/0072 , G02B21/008
摘要： A laser scanning microscope, preferably a confocal laser scanning microscope, and a method for reference correction for a laser scanning microscope, in particular for a confocal laser scanning microscope, having an illumination beam path extending between a laser light source and a specimen, and a detection beam path extending between the specimen and a detection device, is characterized, for error correction with at least one reference beam path used for reference measurement, in that reference light can be coupled out of the illumination beam path into the reference beam path, and that the reference light is qualitatively and/or quantitatively detectable by a detection device.
摘要翻译：激光扫描显微镜，优选共焦激光扫描显微镜，以及用于激光扫描显微镜，特别是用于共聚焦激光扫描显微镜的参考校正方法，其具有在激光光源和样本之间延伸的照明光束路径，以及其特征在于用于用于参考测量的至少一个参考光束路径进行纠错，因为参考光可以从照明光束路径耦合到参考光束路径中，以及参考光是通过检测装置定性和/或定量检测的。

7. 发明授权

US08253937B2 Optical evaluation method by means of laser pulses and corresponding apparatus 有权
标题翻译：光学评估方法采用激光脉冲和相应装置
公开(公告)号：US08253937B2
公开(公告)日：2012-08-28
申请号：US12626057
申请日：2009-11-25
申请人： Vishnu Vardhan Krishnamachari , William C. Hay , Volker Seyfried , Bernd Widzgowski
发明人： Vishnu Vardhan Krishnamachari , William C. Hay , Volker Seyfried , Bernd Widzgowski
IPC分类号： G01J3/44
CPC分类号： G01N21/63
摘要： An optical evaluation method and an apparatus for performing said method are described. First laser pulses of a first type and second laser pulses of a second type that differs from the first type are sent onto a sample to be examined. The sample is hit with first incident light from the two laser pulses in at least one manner of simultaneously, within a very short time lag between the two laser pulses, and a time-correlated manner of the two laser pulses, thereby generating a first optical signal, and hit with second incident light from the two laser pulses, thereby generating a second optical signal. The generated first and second optical signals are detected with at least one detector; and an electronic difference between the first and second optical signals is generated.
摘要翻译：描述了用于执行所述方法的光学评估方法和装置。第一类型的第一激光脉冲和第二类型的与第一类型不同的第二激光脉冲被发送到要检查的样品上。在两个激光脉冲之间的非常短的时间间隔内，以两个激光脉冲的时间相关方式，以至少一种方式同时来自两个激光脉冲的第一入射光和第二激光脉冲的时间相关方式，信号，并用来自两个激光脉冲的第二入射光击中，从而产生第二光信号。产生的第一和第二光信号用至少一个检测器检测; 并且产生第一和第二光信号之间的电子差异。

8. 发明授权

US06483103B2 Apparatus for selecting and detecting at least one spectral region of a spectrally spread light beam 有权
标题翻译：用于选择和检测光谱扩展光束的至少一个光谱区域的装置
公开(公告)号：US06483103B2
公开(公告)日：2002-11-19
申请号：US09783220
申请日：2001-02-15
申请人： Johann Engelhardt , William C. Hay , Juergen Hoffmann
发明人： Johann Engelhardt , William C. Hay , Juergen Hoffmann
IPC分类号： G01J328
CPC分类号： G02B21/0064 , G01J3/32
摘要： An apparatus for selecting and detecting at least one spectral region of a spectrally spread light beam, preferably in the beam path of a confocal scanning microscope, the spread light beam being focussable in a focal line, is characterized, for non-overlapping detection of the spectrally spread light beam of the selected spectral regions in the context of an increased number of detectors and an error-tolerant arrangement, in that there is arranged in the spread light beam an optical component which reflects and/or refracts the light beam to a detector and whose optically effective region becomes smaller or larger along the surface, so that by orientation of the component with respect to the focal line and the resulting superposition of the focal line and surface, the spectral region arriving at the detector is definable.
摘要翻译：一种用于选择和检测光谱扩展光束的至少一个光谱区域的装置，优选地在共聚焦扫描显微镜的光束路径中，扩散光束可聚焦在焦线中，其特征在于，对于不重叠的在增加数量的检测器和容错布置的上下文中，所选择的光谱区域的光谱扩展光束，其中在扩展光束中布置了将光束反射和/或折射到检测器的光学部件并且其光学有效区域沿着表面变得更小或更大，使得通过部件相对于焦线的取向以及所得焦点线和表面的叠加，到达检测器的光谱区域是可定义的。

9. 发明申请

US20110273768A1 TUNABLE MULTIPLE LASER PULSE SCANNING MICROSCOPE AND METHOD OF OPERATING THE SAME 有权
标题翻译：多功能激光脉冲扫描显微镜及其操作方法
公开(公告)号：US20110273768A1
公开(公告)日：2011-11-10
申请号：US12775395
申请日：2010-05-06
申请人： Vishnu Vardhan Krishnamachari , William C. Hay
发明人： Vishnu Vardhan Krishnamachari , William C. Hay
IPC分类号： G02B21/06 , G02F1/39
CPC分类号： G02B21/0032 , G02B21/0076
摘要： A tunable multiple laser pulse scanning microscope and a method of operating the same is described, applying two pulsed laser beams with distinct wavelengths incident on a scanning spot of a sample to be imaged simultaneously or at a specific time delay. The microscope comprises at least two pulsed laser light sources emitting laser light of distinct wavelengths, an acousto-optic tunable filter (AOTF) for tuning at least one of the laser pulses, a delay stage provided upstream of the AOTF, and an actuator for moving delay stage depending on the time delay. As a result, the wavelength of at least one type of pulses is tuned, and the delay between at least two pulses of distinct wavelengths is adjusted.
摘要翻译：描述了可调多激光脉冲扫描显微镜及其操作方法，应用两个具有不同波长的脉冲激光束入射到要同时成像的样品的扫描点或者以特定的时间延迟。显微镜包括至少两个发射不同波长的激光的脉冲激光光源，用于调谐至少一个激光脉冲的声光可调谐滤波器（AOTF），设置在AOTF上游的延迟级，以及用于移动的致动器延迟时间取决于时间延迟。结果，调谐至少一种类型的脉冲的波长，并且调整不同波长的至少两个脉冲之间的延迟。

10. 发明授权

US07483207B2 Apparatus for mounting multiple lasers, and microscope 有权
标题翻译：用于安装多个激光器的装置和显微镜
公开(公告)号：US07483207B2
公开(公告)日：2009-01-27
申请号：US11610122
申请日：2006-12-13
申请人： Rafael Storz , William C. Hay
发明人： Rafael Storz , William C. Hay
IPC分类号： G02B21/06 , G02B21/00
CPC分类号： G02B21/0032 , G01J3/02 , G01J3/0218 , G01J3/0291 , G01J3/10
摘要： An apparatus for mounting for multiple lasers (16a, 16b, 16c) is disclosed. The multiple lasers (16a, 16b, 16c) generate light that is guided via a light-guiding fiber to an optical system (10). Provided for that purpose on the mounting plate (15) is a combining unit (18) that comprises multiple input ports (18a, 18b, 18c). Each of the lasers (16a, 16b, 16c) possesses an output (25a, 25b, 25c) for its light, each laser (16a, 16b, 16c) being mounted on the mounting plate (15) in such a way that the respective output (25a, 25b, 25c) is colinear with the respective pertinent input port (18a, 18b, 18c) of the combining unit (18). The combining unit (18) combines the light of the multiple lasers (16a, 16b, 16c) into a single beam (30).
摘要翻译：公开了一种用于多个激光器（16a，16b，16c）的安装装置。多个激光器（16a，16b，16c）产生经由光导纤维被引导到光学系统（10）的光。为此，在安装板（15）上设置有包括多个输入端口（18a，18b，18c）的组合单元（18）。每个激光器（16a，16b，16c）具有用于其光的输出（25a，25b，25c），每个激光器（16a，16b，16c）以这样的方式安装在安装板（15）上，输出（25a，25b，25c）与组合单元（18）的相应输入端口（18a，18b，18c）共线。组合单元（18）将多个激光器（16a，16b，16c）的光组合成单个光束（30）。

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