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    • 3. 发明授权
    • Immobilized biocatalysts
    • 固定生物催化剂
    • US4518693A
    • 1985-05-21
    • US437945
    • 1982-11-01
    • Wei-Youh Kuu
    • Wei-Youh Kuu
    • C12N11/04C12N11/10C12P7/06
    • C12N11/10C12N11/04C12P7/06Y02E50/17
    • Biocatalysts such as microbial cells are immobilized by forming spherical gel beads containing the microbial cells from a hydrogel such as agar or carrageenan, incubating the beads for a time sufficient to permit the microbial cells to produce CO.sub.2 and thereby decrease resistance of the beads to diffusion, diffusing into the beads a monomer, cross-linking agent and accelerator and contacting the beads with a polyermization initiator to cause polymerization of the monomer. The polymerized monomer prevents breakup characteristic of hydrogels containing growing microbial cells. This method is particularly suitable for the immobilization of microbial cells for use in fermentation to produce ethanol.
    • 通过从水凝胶如琼脂或角叉菜胶形成含有微生物细胞的球形凝胶珠来固定生物催化剂,将珠子孵育足够的时间以使微生物细胞产生CO 2,从而降低珠粒对扩散的抵抗力, 将单体,交联剂和促进剂扩散入珠中,并使珠与多元引发剂接触以引起单体的聚合。 聚合的单体防止含有生长的微生物细胞的水凝胶的破裂特性。 该方法特别适用于固定用于发酵的微生物细胞以产生乙醇。