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1. 发明授权

US09017976B2 Engineering microbes for efficient production of chemicals 有权
标题翻译：用于高效生产化学品的工程微生物
公开(公告)号：US09017976B2
公开(公告)日：2015-04-28
申请号：US13503999
申请日：2010-11-17
申请人： Wei Gong , Sudhanshu Dole , Tammy Grabar , Andrew Christopher Collard , Janice G. Pero , R. Rogers Yocum
发明人： Wei Gong , Sudhanshu Dole , Tammy Grabar , Andrew Christopher Collard , Janice G. Pero , R. Rogers Yocum
IPC分类号： B29C70/04 , C12N15/74 , C12N1/00 , A61B6/04 , B29L31/00
CPC分类号： A61B6/0442 , B29C70/04 , B29L2031/753 , C12N9/1205 , C12N9/88 , C12N15/52 , C12P7/40 , C12P7/46
摘要： This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.
摘要翻译：本发明涉及从遗传工程和代谢进化的微生物生产化学物质。已经建立了化学生产的改进，并确定了导致这些改进的特定突变。在鉴定在遗传工程化生产琥珀酸的细菌菌株的代谢进化期间发生的突变的具体实例。本发明还提供了用于评估在增加的琥珀酸生产的代谢进化期间选择的突变的工业适用性的方法。本发明进一步提供了工程改造成具有在代谢进化期间选择的突变的微生物，并有助于改善琥珀酸，其它有机酸和其它商业利益的化学品的生产。

2. 发明申请

US20120220000A1 ENGINEERING MICROBES FOR EFFICIENT PRODUCTION OF CHEMICALS 有权
标题翻译：工程微生物高效生产化学品
公开(公告)号：US20120220000A1
公开(公告)日：2012-08-30
申请号：US13503999
申请日：2010-11-17
申请人： Wei Gong , Sudhanshu Dole , Tammy Grabar , Andrew Chirstopher Collard , Janice G. Pero , R. Rogers Yocum
发明人： Wei Gong , Sudhanshu Dole , Tammy Grabar , Andrew Chirstopher Collard , Janice G. Pero , R. Rogers Yocum
IPC分类号： C12P7/46 , C12N15/74 , C12N1/00
CPC分类号： A61B6/0442 , B29C70/04 , B29L2031/753 , C12N9/1205 , C12N9/88 , C12N15/52 , C12P7/40 , C12P7/46
摘要： This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.
摘要翻译：本发明涉及从遗传工程和代谢进化的微生物生产化学物质。已经建立了化学生产的改进，并确定了导致这些改进的特定突变。在鉴定在遗传工程化生产琥珀酸的细菌菌株的代谢进化期间发生的突变的具体实例。本发明还提供了用于评估在增加的琥珀酸生产的代谢进化期间选择的突变的工业适用性的方法。本发明进一步提供了工程改造成具有在代谢进化期间选择的突变的微生物，并有助于改善琥珀酸，其它有机酸和其它商业利益的化学品的生产。

3. 发明授权

US08871489B2 Metabolic evolution of Escherichia coli strains that produce organic acids 有权
标题翻译：产生有机酸的大肠杆菌菌株的代谢进化
公开(公告)号：US08871489B2
公开(公告)日：2014-10-28
申请号：US13394176
申请日：2010-11-17
申请人： Tammy Grabar , Wei Gong , R. Rogers Yocum
发明人： Tammy Grabar , Wei Gong , R. Rogers Yocum
IPC分类号： C12N1/20 , C12N1/00 , C12N15/74 , C07H21/04 , C07K14/245 , C12P7/46 , C12P7/54 , C12P7/40
CPC分类号： C07K14/245 , C12P7/40 , C12P7/46 , C12P7/54
摘要： This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.
摘要翻译：本发明涉及先前优化用于在发酵条件下以商业上显着的量生产有机酸的微生物生物的代谢进化，其中使用己糖作为最小矿物培养基中的唯一碳源。作为这种代谢进化的结果，微生物生物体获得使用来自纤维素材料的戊糖的生长能力，同时保持原始生长动力学，有机酸生产速率和使用己糖作为碳源的能力。本发明还公开了赋予在生产商业上大量有机酸中同时使用己糖和戊糖的能力的微生物中的遗传变化。

4. 发明申请

US20120202259A1 METABOLIC EVOLUTION OF ESCHERICHIS COLI STRAINS THAT PRODUCE ORGANIC ACIDS 有权
标题翻译：生产有机酸的ESCHERICHIS COLI菌株的代谢演化
公开(公告)号：US20120202259A1
公开(公告)日：2012-08-09
申请号：US13394176
申请日：2010-11-17
申请人： Tammy Grabar , Wei Gong , R. Rogers Yocum
发明人： Tammy Grabar , Wei Gong , R. Rogers Yocum
IPC分类号： C12P7/40 , C12N1/21
CPC分类号： C07K14/245 , C12P7/40 , C12P7/46 , C12P7/54
摘要： This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.
摘要翻译：本发明涉及先前优化用于在发酵条件下以商业上显着的量生产有机酸的微生物生物的代谢进化，其中使用己糖作为最小矿物培养基中的唯一碳源。作为这种代谢进化的结果，微生物生物体获得使用来自纤维素材料的戊糖的生长能力，同时保持原始生长动力学，有机酸生产速率和使用己糖作为碳源的能力。本发明还公开了赋予在生产商业上大量有机酸中同时使用己糖和戊糖的能力的微生物中的遗传变化。

5. 发明申请

US20090298137A1 MICROORGANISM AND PROCESS FOR THE PREPARATION OF L-METHIONINE 失效
标题翻译：用于制备L-甲硫氨酸的微生物和方法
公开(公告)号：US20090298137A1
公开(公告)日：2009-12-03
申请号：US12091948
申请日：2006-10-23
申请人： Oskar Zelder , Andreas Herold , Corinna Klopprogge , Hartwig Schröder , Stefan Haefner , Elmar Heinzle , Christoph Wittmann , Jens Kroemer , Janice G. Pero , R. Rogers Yocum , Thomas A. Patterson , Mark Williams , Theron Herman
发明人： Oskar Zelder , Andreas Herold , Corinna Klopprogge , Hartwig Schröder , Stefan Haefner , Elmar Heinzle , Christoph Wittmann , Jens Kroemer , Janice G. Pero , R. Rogers Yocum , Thomas A. Patterson , Mark Williams , Theron Herman
IPC分类号： C12P13/12 , C12N1/21
CPC分类号： C12P13/12 , A23K10/16 , A23K20/142 , A23K40/10 , C12N15/52
摘要： The present invention relates to microorganisms and processes for the efficient preparation of L-amino acids such as L-methionine. In particular, the present invention relates to microorganisms and processes in which the formation and/or accumulation of homolanthionine in the methionine pathway is reduced and/or prevented.
摘要翻译：本发明涉及用于有效制备L-氨基酸如L-甲硫氨酸的微生物和方法。特别地，本发明涉及微生物和其中甲硫氨酸途径中高山ion素的形成和/或积累减少和/或预防的方法。

6. 发明授权

US07291489B2 Microorganisms and processes for enhanced production of pantothenate 有权
标题翻译：用于增加泛酸盐生产的微生物和方法
公开(公告)号：US07291489B2
公开(公告)日：2007-11-06
申请号：US10614333
申请日：2003-07-03
申请人： R. Rogers Yocum , Thomas A. Patterson , Janice G. Pero , Theron Hermann
发明人： R. Rogers Yocum , Thomas A. Patterson , Janice G. Pero , Theron Hermann
IPC分类号： C12P13/04 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N15/52 , C12P13/02
摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
摘要翻译：本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。还具有重组微生物和培养条件。还有这些微生物产生的组合物。

7. 发明授权

US08232081B2 Methods and microorganisms for production of panto-compounds 有权
标题翻译：泛化合物生产方法和微生物
公开(公告)号：US08232081B2
公开(公告)日：2012-07-31
申请号：US10984449
申请日：2004-11-08
申请人： R. Rogers Yocum , Thomas A. Patterson , Theron Hermann , Janice G. Pero
发明人： R. Rogers Yocum , Thomas A. Patterson , Theron Hermann , Janice G. Pero
IPC分类号： C12P13/04 , C12P13/02 , C12P7/42 , C12P7/18
CPC分类号： C12P17/04 , C12N15/52 , C12P13/02
摘要： The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate α-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.
摘要翻译：本发明的特征在于使用其中泛酸生物合成途径和/或异亮氨酸 - 缬氨酸生物合成途径和/或辅酶A生物合成途径已经被操纵的微生物来生产泛化合物（例如泛酸盐）的方法。提供了酮过氧还原酶过表达微生物以及天冬氨酸α-脱羧酶过表达微生物的方法。描述了以前体非依赖性方式和高产率生产泛化合物的方法。还提供了用于实践上述方法的重组微生物，载体，分离的核酸分子，基因和基因产物。本发明还具有以前未鉴定的微生物泛酸激酶基因coaX，以及利用具有改良的泛酸激酶活性的微生物产生泛化合物的方法。重组微生物，载体，分离的coaX核酸分子和纯化的CoaX蛋白。还特征在于利用本发明的重组微生物和/或纯化的CoaX蛋白鉴定泛酸激酶调节剂的方法。

8. 发明申请

US20100003727A1 Coryneform Bacteria with Formate-THF-Synthetase and/or Glycine Cleavage Activity 审中-公开
标题翻译：具有甲酸-THF合成酶和/或甘氨酸裂解活性的棒状杆菌细菌
公开(公告)号：US20100003727A1
公开(公告)日：2010-01-07
申请号：US12519909
申请日：2008-02-14
申请人： Oskar Zelder , Hartwig Schröder , Corinna Klopprogge , Andrea Herold , Stefan Haefner , Thomas A. Patterson , R. Rogers Yocum , Janice G. Pero
发明人： Oskar Zelder , Hartwig Schröder , Corinna Klopprogge , Andrea Herold , Stefan Haefner , Thomas A. Patterson , R. Rogers Yocum , Janice G. Pero
IPC分类号： C12P13/12 , C12N1/21
CPC分类号： C12P13/12 , C12N15/52
摘要： The present invention relates to microorganisms, in particular C. glutamicum in which the formation of N5,N10-methylene-THF is increased.The present invention also relates to the use of such microorganisms for producing methionine.
摘要翻译：本发明涉及增加N5，N10-亚甲基-THF的形成的微生物，特别是谷氨酸棒杆菌。本发明还涉及这种微生物用于产生甲硫氨酸的用途。

9. 发明申请

US20090298136A1 Methionine producing recombinant microorganisms 审中-公开
标题翻译：产生甲硫氨酸的重组微生物
公开(公告)号：US20090298136A1
公开(公告)日：2009-12-03
申请号：US11988962
申请日：2006-07-18
申请人： Oskar Zelder , Stefan Haefner , Corinna Klopprogge , Hartwig Schroder , Andrea Herold , Thomas A. Patterson , Theron Hermann , R. Rogers Yocum , Mark K. William , Janice G. Pero
发明人： Oskar Zelder , Stefan Haefner , Corinna Klopprogge , Hartwig Schroder , Andrea Herold , Thomas A. Patterson , Theron Hermann , R. Rogers Yocum , Mark K. William , Janice G. Pero
IPC分类号： C12P13/12 , C12N1/21
CPC分类号： C12P13/12 , C12N15/52
摘要： This invention relates to methionine producing recombinant microorganisms. Specifically, this invention relates to recombinant strains of Corynebacterium that produce increased levels of methionine compared to their wild-type counterparts and further to methods of generating such microorganisms.
摘要翻译：本发明涉及产生甲硫氨酸的重组微生物。具体而言，本发明涉及棒状杆菌的重组菌株，其与野生型对应物相比产生较高水平的甲硫氨酸，并且还涉及产生这种微生物的方法。

10. 发明授权

US06841366B1 Biotin biosynthesis in bacillus subtilis 失效
标题翻译：枯草杆菌中的生物素生物合成
公开(公告)号：US06841366B1
公开(公告)日：2005-01-11
申请号：US09407728
申请日：1999-09-28
申请人： Stanley Grant Bower , John B. Perkins , R. Rogers Yocum , Janice G. Pero
发明人： Stanley Grant Bower , John B. Perkins , R. Rogers Yocum , Janice G. Pero
IPC分类号： C12N15/09 , C07K14/32 , C12N1/21 , C12N15/31 , C12N15/52 , C12N15/75 , C12P17/18 , C12R1/125 , C12R1/19 , C12N9/00
CPC分类号： C12R1/125 , C07K14/32 , C12N15/52 , C12N15/75 , C12P17/186
摘要： The invention generally provides the genes of the biotin biosynthetic operon of Bacillus subtilis, and closely related species thereof, and well as constructions useful for high level production of biotin.
摘要翻译：本发明通常提供枯草芽孢杆菌的生物素生物合成操纵子的基因及其密切相关的物种，以及可用于高水平生产生物素的结构。

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