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1. 发明授权

US5985650A Method of desulfurization of fossil fuel with flavoprotein 失效
标题翻译：化石燃料与黄素蛋白脱硫的方法
公开(公告)号：US5985650A
公开(公告)日：1999-11-16
申请号：US25527
申请日：1998-02-18
申请人： Charles H. Squires , Wan Ji , Lei Xi , Beatrice C. Ortego , Olga S. Pogrebinsky , Kevin A. Gray , John D. Childs
发明人： Charles H. Squires , Wan Ji , Lei Xi , Beatrice C. Ortego , Olga S. Pogrebinsky , Kevin A. Gray , John D. Childs
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02
CPC分类号： C12N9/0036 , C10G32/00 , C12P1/00
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of:a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture;b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; andc) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.
摘要翻译：本发明涉及通过向生物催化剂中加入黄素蛋白来提高化石燃料脱硫反应速率的发现。本发明涉及提高含有机硫化合物的化石燃料脱硫速率的方法，包括以下步骤：a）使化石燃料与含有能够裂解碳 - 硫键的生物催化剂的水相接触，增加量的黄素蛋白，从而形成化石燃料和水相混合物; b）将步骤（a）的混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和c）从所得水相中分离具有降低的有机硫含量的化石燃料。本发明还涉及含有一种或多种重组DNA分子的重组微生物，所述重组DNA分子编码能够脱硫含有有机硫分子的化石燃料并且编码黄素蛋白的生物催化剂。本发明还涉及包含（a）能够使含有有机硫分子的化石燃料脱硫的生物催化剂和（b）黄素蛋白的组合物。

2. 发明申请

US20080268437A1 Method of Targeted and Comprehensive Sequencing Using High-Density Oligonucleotide Array 审中-公开
标题翻译：使用高密度寡核苷酸阵列的靶向和综合测序方法
公开(公告)号：US20080268437A1
公开(公告)日：2008-10-30
申请号：US11664023
申请日：2005-09-27
申请人： Wan Ji , Wenli Zhou , Sara F. Davis , Scott K. Davis
发明人： Wan Ji , Wenli Zhou , Sara F. Davis , Scott K. Davis
IPC分类号： C12Q1/68 , C40B20/04 , C12P19/34
CPC分类号： C12Q1/6809 , C12Q1/6837
摘要： A method for targeted and comprehensive sequencing using high-density oligonucleotide array, comprising the steps of hybridizing nucleic acid from an investigative species with high-density oligonucleotide arrays of a related species, identifying the oligonucleotide probes that generate high hybridization signals, using the probes sequences to make PCR primers, amplifying heterologous genes by PCR with the gene specific PCR primers and an anchoring primer, and sequencing the PCR products.
摘要翻译：一种用于使用高密度寡核苷酸阵列进行靶向和全面测序的方法，包括以下步骤：使用探针序列将来自研究物种的核酸与相关物种的高密度寡核苷酸阵列杂交，鉴定产生高杂交信号的寡核苷酸探针制作PCR引物，用基因特异性PCR引物和锚定引物通过PCR扩增异源基因，并对PCR产物进行测序。

3. 发明申请

US20060246503A1 Method for rapid amplification of DNA 审中-公开
公开(公告)号：US20060246503A1
公开(公告)日：2006-11-02
申请号：US11483855
申请日：2006-07-10
申请人： Wan Ji , Keqin Gregg , Bonnie Reus , Jon Kemppainen , Kristen Taylor , Scott Davis
发明人： Wan Ji , Keqin Gregg , Bonnie Reus , Jon Kemppainen , Kristen Taylor , Scott Davis
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q1/6846 , C12Q1/686 , C12Q2525/179 , C12Q2525/155
摘要： The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3′ end and a generic sequence 5′ of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.

4. 发明申请

US20080131886A1 METHOD FOR RAPID AMPLIFICATION OF DNA 审中-公开
标题翻译： DNA快速扩增方法
公开(公告)号：US20080131886A1
公开(公告)日：2008-06-05
申请号：US11741596
申请日：2007-04-27
申请人： Wan Ji , Keqin Gregg , Bonnie Reus , Jon Kemppainen , Kristen Taylor , Scott Davis
发明人： Wan Ji , Keqin Gregg , Bonnie Reus , Jon Kemppainen , Kristen Taylor , Scott Davis
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6846 , C12Q1/686 , C12Q2525/179 , C12Q2525/155
摘要： The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3′ end and a generic sequence 5′ of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.
摘要翻译：本公开内容涉及具有在其3'端具有核苷酸随机序列和随机核苷酸的通用序列5'的第一引物的DNA扩增方法，以及具有第一引物通用序列的第二引物。本公开还涉及在固体培养基上沉淀DNA以改善DNA扩增的方法。在优选的实施方案中，本公开的方法用于DNA样品（例如血迹或痕量DNA）的高通量基因分型。

5. 发明申请

US20050009015A1 Method for relative quantification of attached nucleic acids 审中-公开
标题翻译：连接核酸相对定量的方法
公开(公告)号：US20050009015A1
公开(公告)日：2005-01-13
申请号：US10185592
申请日：2002-06-27
申请人： Wan Ji , Keqin Gregg , Bonnie Reus , Jon Kemppainen , Scott Davis
发明人： Wan Ji , Keqin Gregg , Bonnie Reus , Jon Kemppainen , Scott Davis
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566 , G01N33/58
CPC分类号： C12Q1/6827 , C12Q2525/161 , C12Q2565/501 , C12Q2563/155 , C12Q2521/501
摘要： A method and associated compositions for the relative quantification of nucleic acid on an address-defined surface, involving fitting the nucleic acid with a generic oligonucleotide, and hybridizing the generic oligonucleotide with a directly or indirectly labeled complementary oligonucleotide. The method is applicable, for example, to SNP genotyping and gene expression analysis.
摘要翻译：一种用于在地址定义表面上相对定量核酸的方法和相关组合物，涉及用一般寡核苷酸拟合核酸，并将该通用寡核苷酸与直接或间接标记的互补寡核苷酸杂交。该方法适用于例如SNP基因分型和基因表达分析。

6. 发明授权

US06638722B2 Method for rapid amplification of DNA 有权
标题翻译：快速扩增DNA的方法
公开(公告)号：US06638722B2
公开(公告)日：2003-10-28
申请号：US09881565
申请日：2001-06-13
申请人： Wan Ji , Keqin Gregg , Bonnie Reus , Jon Kemppainen , Kristen Taylor , Scott Davis
发明人： Wan Ji , Keqin Gregg , Bonnie Reus , Jon Kemppainen , Kristen Taylor , Scott Davis
IPC分类号： C12Q168
CPC分类号： C12Q1/6858 , C12Q1/6846 , C12Q1/686 , C12Q2525/179 , C12Q2525/155
摘要： The present disclosure relates to methods of DNA amplification with a first primer that has a random sequence of nucleotides at its 3′ end and a generic sequence 5′ of the random nucleotides, as well as a second primer with the generic sequence of the first primer. The disclosure further relates to a method of precipitating DNA on a solid medium to improve DNA amplification. In a preferred embodiment, the presently disclosed methods are used for high-throughput genotyping of DNA samples, such as bloodstains or trace amounts of DNA.
摘要翻译：本公开内容涉及具有在其3'端具有核苷酸随机序列和随机核苷酸的通用序列5'的第一引物的DNA扩增方法，以及具有第一引物通用序列的第二引物。本公开还涉及在固体培养基上沉淀DNA以改善DNA扩增的方法。在优选的实施方案中，本公开的方法用于DNA样品（例如血迹或痕量DNA）的高通量基因分型。

7. 发明授权

US5733773A Method of desulfurization of fossil fuel with flavoprotein 失效
公开(公告)号：US5733773A
公开(公告)日：1998-03-31
申请号：US662810
申请日：1996-06-12
申请人： Charles H. Squires , Wan Ji , Lei Xi , Beatrice C. Ortego , Olga S. Pogrebinsky , Kevin A. Gray , John D. Childs
发明人： Charles H. Squires , Wan Ji , Lei Xi , Beatrice C. Ortego , Olga S. Pogrebinsky , Kevin A. Gray , John D. Childs
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02 , C12N1/20
CPC分类号： C12N9/0036 , C10G32/00 , C12P1/00
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.

8. 发明授权

US5321111A Method for extracting polyisoprenes from plants 失效
标题翻译：从植物中提取聚异戊二烯的方法
公开(公告)号：US5321111A
公开(公告)日：1994-06-14
申请号：US127808
申请日：1993-09-28
申请人： Wan Ji
发明人： Wan Ji
IPC分类号： C08C4/00 , C08G83/00
CPC分类号： C08C4/00 , Y10S528/93 , Y10S528/931
摘要： A method for extracting polyisoprenes from cells, comprising the steps of contacting the cells with an organic protein-denaturing compound, rupturing the cells to release the polyisoprene from the cells and recovering the polyisoprene. The preferred protein denaturing compound comprises an aqueous solution of a guanidine salt.
摘要翻译：一种从细胞中提取聚异戊二烯的方法，包括以下步骤：使细胞与有机蛋白质变性化合物接触，破裂细胞以从细胞中释放聚异戊二烯并回收聚异戊二烯。优选的蛋白质变性化合物包括胍盐的水溶液。

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