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1. 发明授权

US07425423B1 Thermostable nucleic acid polymerase from Thermococcus gorgonarius 失效
标题翻译：来自Thermococcus gorgonarius的热稳定性核酸聚合酶
公开(公告)号：US07425423B1
公开(公告)日：2008-09-16
申请号：US09269860
申请日：1997-10-01
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12 , C12Q1/48
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定酶源自嗜热古细菌热球菌。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

2. 发明申请

US20090093043A1 THERMOSTABLE NUCLEIC ACID POLYMERASE FROM THERMOCOCCUS GORGONARIUS 失效
标题翻译：热稳定性核酸聚合酶
公开(公告)号：US20090093043A1
公开(公告)日：2009-04-09
申请号：US12135994
申请日：2008-06-09
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N1/21 , C12N15/11 , C12N15/00
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定性酶源自嗜热古细菌Thermococcus gorgonarius。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

3. 发明授权

US06468775B1 Thermostable DNA polymerase from carboxydothermus hydrogenoformans 有权
标题翻译：来自羧酸热霉菌的热稳定DNA聚合酶
公开(公告)号：US06468775B1
公开(公告)日：2002-10-22
申请号：US09269861
申请日：1999-11-22
申请人： Waltraud Ankenbauer , Ursula Markau , Vitaly Svetlichny , Gudrun Schmitz-Agheguian , Astrid Reiser , Bernhard Angerer , Christine Ebenbichler , Frank Laue , Elizaveta Bonch-Osmolovskaya
发明人： Waltraud Ankenbauer , Ursula Markau , Vitaly Svetlichny , Gudrun Schmitz-Agheguian , Astrid Reiser , Bernhard Angerer , Christine Ebenbichler , Frank Laue , Elizaveta Bonch-Osmolovskaya
IPC分类号： C12N912
CPC分类号： C12N9/1252
摘要： A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3′-5′ exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.
摘要翻译：提供来自嗜热菌的DNA聚合酶。 DNA聚合酶显示镁离子依赖的逆转录酶活性和3'-5'核酸外切酶活性。本发明还包括能够产生酶的重组质粒和转化的宿主细胞。该酶被分类为EC 2.7.7.7。DNA，DNA核苷酸转移酶DNA指导型。

4. 发明授权

US08008054B2 Thermostable nucleic acid polymerase from Thermococcus gorgonarius 有权
标题翻译：来自Thermococcus gorgonarius的热稳定性核酸聚合酶
公开(公告)号：US08008054B2
公开(公告)日：2011-08-30
申请号：US12793602
申请日：2010-06-03
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定性酶源自嗜热古细菌Thermococcus gorgonarius。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

5. 发明申请

US20110020898A1 Thermostable Nucleic Acid Polymerase From Thermococcus Gorgonarius 有权
标题翻译：热稳定性核糖核酸聚合酶
公开(公告)号：US20110020898A1
公开(公告)日：2011-01-27
申请号：US12793602
申请日：2010-06-03
申请人： Waltraud ANKENBAUER , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud ANKENBAUER , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定性酶源自嗜热古细菌Thermococcus gorgonarius。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

6. 发明授权

US07759107B2 Thermostable nucleic acid polymerase from Thermococcus gorgonarius 失效
标题翻译：来自Thermococcus gorgonarius的热稳定性核酸聚合酶
公开(公告)号：US07759107B2
公开(公告)日：2010-07-20
申请号：US12135994
申请日：2008-06-09
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12 , C12N1/20 , C12Q1/48
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定性酶源自嗜热古细菌Thermococcus gorgonarius。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

7. 发明申请

US20090269766A1 NUCLEIC ACID AMPLIFICATION IN THE PRESENCE OF MODIFIED RANDOMERS 审中-公开
标题翻译：核素放射治疗存在改良型放射治疗
公开(公告)号：US20090269766A1
公开(公告)日：2009-10-29
申请号：US12406450
申请日：2009-03-18
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12Q1/68 , C12N9/00 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译：本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机疏水部分这样的组合物特别适用于进行热启动PCR。

8. 发明申请

US20090181401A1 Detection format for hot start real time polymerase chain reaction 审中-公开
标题翻译：检测格式为热启动实时聚合酶链反应
公开(公告)号：US20090181401A1
公开(公告)日：2009-07-16
申请号：US12407339
申请日：2009-03-19
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译：本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应，温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光至少在多个放大循环之后。

9. 发明申请

US20050037410A1 Detection format for hot start real time polymerase chain reaction 审中-公开
标题翻译：检测格式为热启动实时聚合酶链反应
公开(公告)号：US20050037410A1
公开(公告)日：2005-02-17
申请号：US10903992
申请日：2004-07-30
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： G01N33/58 , C12N15/09 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译：本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应，温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光至少在多个放大循环之后。

10. 发明申请

US20130109060A1 Nucleic Acid Amplification in the Presence of Modified Randomers 失效
标题翻译：核酸扩增在修饰的兰姆斯存在下
公开(公告)号：US20130109060A1
公开(公告)日：2013-05-02
申请号：US13711846
申请日：2012-12-12
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译：本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机疏水部分这样的组合物特别适用于进行热启动PCR。

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