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1. 发明申请

US20200291362A1 PROTEIN-INDUCED PLURIPOTENT CELL TECHNOLOGY AND USES THEREOF 审中-公开
公开(公告)号：US20200291362A1
公开(公告)日：2020-09-17
申请号：US16849515
申请日：2020-04-15
申请人： Wayne State University
发明人： Jianjun Wang , Qianqian Li
IPC分类号： C12N5/074
摘要： A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPCS cells formed using this method and uses thereof.

2. 发明申请

US20190336562A1 METHOD AND COMPOSITION FOR A PROTEIN TRANSDUCTION TECHNOLOGY AND ITS APPLICATIONS 审中-公开
公开(公告)号：US20190336562A1
公开(公告)日：2019-11-07
申请号：US16517880
申请日：2019-07-22
申请人： Wayne State University
发明人： Jianjun Wang , Qianqian Li
IPC分类号： A61K38/00 , G01N33/50 , G01N33/68 , C12P21/00 , G01N33/58 , C12N15/88 , A61K47/58 , A61K47/54
摘要： A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.

3. 发明授权

US10221397B2 Protein-induced pluripotent cell technology and uses thereof 有权
公开(公告)号：US10221397B2
公开(公告)日：2019-03-05
申请号：US15808205
申请日：2017-11-09
申请人： Wayne State University
发明人： Jianjun Wang , Qianqian Li
IPC分类号： C12N5/074
摘要： A method of generating protein-induced pluripotent stem cells by delivering bacterial!y expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPCS cells formed using this method and uses thereof.

4. 发明授权

US10640751B2 Protein-induced pluripotent cell technology and uses thereof 有权
公开(公告)号：US10640751B2
公开(公告)日：2020-05-05
申请号：US16271150
申请日：2019-02-08
申请人： Wayne State University
发明人： Jianjun Wang , Qianqian Li
IPC分类号： C12N5/074
CPC分类号： C12N5/0696 , C12N2501/115 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2506/1307
摘要： A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPS cells formed using this method and uses thereof.

5. 发明授权

US10076574B2 Methods, systems and compositions relating to cell conversion via protein-induced in-vivo cell reprogramming 有权
公开(公告)号：US10076574B2
公开(公告)日：2018-09-18
申请号：US15031839
申请日：2014-10-27
申请人： WAYNE STATE UNIVERSITY , HENRY FORD HEALTH SYSTEM
发明人： Jianjun Wang , Qianqian Li , Michael Chopp , Feng Jiang , Guojun Wu
IPC分类号： A61K38/17 , A61K47/48 , C12N5/074 , A61K48/00 , A61K9/00 , A61K47/54 , A61K47/59 , A61K38/00
CPC分类号： A61K47/59 , A61K9/0019 , A61K38/00 , A61K38/1709 , A61K47/543 , A61K48/0041 , C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/605 , C12N2502/30 , C12N2506/30
摘要： Methods for treating a subject in need thereof are provided which include administering a pharmaceutical composition comprising a protein transduction reagent-modified reprogramming protein to the subject, wherein the protein transduction reagent is non-covalently bound to the reprogramming protein and wherein the protein transduction reagent comprises a cation reagent and a lipid. According to aspects, such methods provide delivery of protein-transduction reagent-modified reprogramming proteins to cancer cells, such as tumor cells, as well as diseased cells of diseased tissues and provide in vivo conversion of diseased cells into normal cells via protein-induced in situ cell reprogramming without administration of nucleic acids to the subject.

6. 发明授权

US11154584B2 Method and composition for a protein transduction technology and its applications 有权
公开(公告)号：US11154584B2
公开(公告)日：2021-10-26
申请号：US16517880
申请日：2019-07-22
申请人： Wayne State University
发明人： Jianjun Wang , Qianqian Li
IPC分类号： A61K38/00 , C12N15/88 , G01N33/50 , A61K47/54 , A61K47/58 , C12P21/00 , G01N33/58 , G01N33/68
摘要： A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.

7. 发明授权

US10548983B2 Methods, systems and compositions relating to cell conversion via protein-induced in-vivo cell reprogramming 有权
公开(公告)号：US10548983B2
公开(公告)日：2020-02-04
申请号：US16120829
申请日：2018-09-04
申请人： Wayne State University
发明人： Jianjun Wang , Qianqian Li , Michael Chopp , Feng Jiang , Guojun Wu
IPC分类号： A61K38/17 , A61K47/59 , C12N5/074 , A61K9/00 , A61K47/54 , A61K48/00 , A61K38/00
摘要： Methods for treating a subject in need thereof are provided which include administering a pharmaceutical composition comprising a protein transduction reagent-modified reprogramming protein to the subject, wherein the protein transduction reagent is non-covalently bound to the reprogramming protein and wherein the protein transduction reagent comprises a cation reagent and a lipid. According to aspects, such methods provide delivery of protein-transduction reagent-modified reprogramming proteins to cancer cells, such as tumor cells, as well as diseased cells of diseased tissues and provide in vivo conversion of diseased cells into normal cells via protein-induced in situ cell reprogramming without administration of nucleic acids to the subject.

8. 发明申请

US20160263233A1 METHODS, SYSTEMS AND COMPOSITIONS RELATING TO CELL CONVERSION VIA PROTEIN-INDUCED IN-VIVO CELL REPROGRAMMING 审中-公开
标题翻译：通过蛋白质诱导的体内细胞转化相关细胞转化的方法，系统和组合物
公开(公告)号：US20160263233A1
公开(公告)日：2016-09-15
申请号：US15031839
申请日：2014-10-27
申请人： WAYNE STATE UNIVERSITY
发明人： Jianjun Wang , Qianqian Li , Michael Chopp , Feng Jiang , Guojun Wu
IPC分类号： A61K47/48 , A61K38/17
CPC分类号： A61K47/48192 , A61K9/0019 , A61K38/00 , A61K38/1709 , A61K47/543 , A61K47/59 , C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/605 , C12N2502/30 , C12N2506/30
摘要： Methods for treating a subject in need thereof are provided which include administering a pharmaceutical composition comprising a protein transduction reagent-modified reprogramming protein to the subject, wherein the protein transduction reagent is non-covalently bound to the reprogramming protein and wherein the protein transduction reagent comprises a cation reagent and a lipid. According to aspects, such methods provide delivery of protein-transduction reagent-modified reprogramming proteins to cancer cells, such as tumor cells, as well as diseased cells of diseased tissues and provide in vivo conversion of diseased cells into normal cells via protein-induced in situ cell reprogramming without administration of nucleic acids to the subject.
摘要翻译：提供了治疗有需要的受试者的方法，其包括向受试者施用包含蛋白质转导试剂修饰的重编程蛋白的药物组合物，其中所述蛋白转导试剂与所述重编程蛋白非共价结合，并且其中所述蛋白转导试剂包含阳离子试剂和脂质。根据方面，这些方法提供蛋白转导试剂修饰的重编程蛋白向癌细胞（例如肿瘤细胞）以及患病组织的患病细胞的递送，并通过蛋白质诱导的病毒细胞体内转化成正常细胞而不向受试者施用核酸的原位细胞重编程。

9. 发明申请

US20140342389A1 METHOD AND COMPOSITION FOR A PROTEIN TRANSDUCTION TECHNOLOGY AND ITS APPLICATIONS 审中-公开
标题翻译：蛋白质转移技术的方法和组合及其应用
公开(公告)号：US20140342389A1
公开(公告)日：2014-11-20
申请号：US14261627
申请日：2014-04-25
申请人： Wayne State University
发明人： Jianjun Wang , Qianqian Li
IPC分类号： G01N33/58 , C12P21/00 , G01N33/68
CPC分类号： A61K38/00 , A61K47/48046 , A61K47/48176 , C12N15/88 , C12P21/00 , G01N33/5023 , G01N33/58 , G01N33/68 , G01N2458/15
摘要： A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery, a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.
摘要翻译：发明了用于将外源蛋白有效递送到哺乳动物细胞中的蛋白质转导方法，其具有靶向不同细胞区室的能力并且防止递送的蛋白质从细胞蛋白酶降解。用于处理蛋白质的组合物在载体中具有阳离子试剂，脂质和增强剂。该方法可以以多种方式使用，包括：使用哺乳动物细胞机制产生大量正确折叠的翻译后修饰蛋白，使用小分子荧光团进行细胞内荧光光谱和成像，并使用细胞内核磁共振光谱活哺乳动物细胞。该方法允许细胞生物学在原子分辨率下与生理和病理相关，并允许蛋白质治疗来治疗人类疾病。该方法还可用于在哺乳动物细胞内递送外源蛋白质，其中外源蛋白质遵循与内源性蛋白质相似的分泌途径。

10. 发明授权

US10357532B2 Method and composition for a protein transduction technology and its applications 有权
公开(公告)号：US10357532B2
公开(公告)日：2019-07-23
申请号：US15637544
申请日：2017-06-29
申请人： Wayne State University
发明人： Jianjun Wang , Qianqian Li
IPC分类号： A61K38/00 , C12N15/88 , G01N33/50 , C12P21/00 , G01N33/58 , G01N33/68 , A61K47/54 , A61K47/58
摘要： A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery, a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.

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