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1. 发明申请

US20170321247A1 CANCER MARKER AND CANCER DETERMINATION METHOD 审中-公开
公开(公告)号：US20170321247A1
公开(公告)日：2017-11-09
申请号：US15501709
申请日：2015-08-18
申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.
发明人： Yukinobu HAYASHIDA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12N15/09 , C12Q1/68 , C12Q1/6809 , C12Q1/6827 , C12Q1/6851 , C12Q1/6874 , C12Q2523/125
摘要： The invention relates to a cancer marker, consisting of a portion of or all of the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell, and being continuous nucleotide sequences comprising at least the nucleotide sequence represented by SEQ ID NO: 2 or 3, wherein 30% or more of cytosines of the -CG- sequences present in said nucleotide sequence are methylated, and method for determining canceration of a cell on the basis of methylation ratio, wherein said methylation ratio is measured by a bisulfate reaction for a portion of or all of cytosines of the -CG- sequences present in the nucleotide sequence represented by SEQ ID NO: 1 in a FOXB2 gene derived from a cell.

2. 发明申请

US20170342490A1 METHOD FOR DETERMINING STATE OF DIFFERENTIATION OF STEM CELLS, AND NOVEL DIFFERENTIATION MARKER USED THEREFOR 审中-公开
公开(公告)号：US20170342490A1
公开(公告)日：2017-11-30
申请号：US15501761
申请日：2015-08-19
申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.
发明人： Yukinobu HAYASHIDA
IPC分类号： C12Q1/68 , C07K14/47
摘要： The invention relates to a method which is capable of determining, at an initial stage of differentiation, the differentiated state of undifferentiated stem cells. The invention provides a method for determining a differentiated state of a cell comprising detecting expression of FOXB2 gene of a stem cell, and determining the differentiated state based on the result, and a differentiation marker selected from mRNA or protein derived from the FOXB2 gene. The invention is applicable to quality management of the stem cells or to methods for preparation and isolation of the differentiated cells. Further, because the differentiated cells can be determined at an initial stage of culture, the invention is useful for an early stage screening of cells and for quality management of the stem cells, and reduction in culturing period and cost reduction in expenditure regarding a culture medium, or the like, can be expected.

3. 发明申请

US20150307930A1 DETECTION METHOD FOR HYDROXYMETHYLATED CYTOSINE IN DNA AND REAGENT KIT FOR DETECTION 审中-公开
标题翻译：用于检测DNA和试剂盒中羟基甲基化胞嘧啶的检测方法
公开(公告)号：US20150307930A1
公开(公告)日：2015-10-29
申请号：US14653590
申请日：2013-12-24
申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.
发明人： Yukinobu HAYASHIDA , Naoyuki YAMAMOTO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q2537/164 , C12Q2563/137
摘要： The present invention is to provide a method for detecting a hydroxymethylated cytosine in DNA and a detection kit therefor. The present invention relates to “a method for detecting the hydroxymethylated cytosine in DNA, which the method comprises: (1) a step in which a single-stranded DNA is contacted with (i) a polyvalent metal oxide or a polyvalent metal acid salt of a metal atom selected from group 6, group 8, group 9 and group 10 of the periodic table, and contacted with (ii) a peroxide selected from persulfuric acid, percarboxylic acids and the salts thereof; (2) a step in which a specific region of the single-stranded DNA treated in (1) is subjected to amplification treatment; (3) a step in which the presence or absence of an objective amplification product obtained in (2) is detected; and (4) a step in which on the basis of the results of (3), the presence or absence of hydroxymethylated cytosine in the specific region of DNA is determined.”, “a reagent kit for detecting hydroxymethylated cytosine in DNA comprising of a reagent including the above polyvalent metal oxide or a polyvalent metal acid salt and the above peroxide”.
摘要翻译：本发明提供一种检测DNA中羟甲基化胞嘧啶的方法及其检测试剂盒。本发明涉及“检测DNA中的羟甲基化胞嘧啶的方法”，该方法包括：（1）将单链DNA与（i）多价金属氧化物或多价金属酸盐选自周期表第6族，第8族，第9族和第10族的金属原子，并与（ii）选自过硫酸，过羧酸及其盐的过氧化物接触; （2）将（1）中处理的单链DNA的特定区域进行扩增处理的步骤; （3）检测在（2）中得到的目标扩增产物的存在或不存在的步骤; 和（4）基于（3）的结果的步骤，确定DNA的特定区域中存在或不存在羟甲基化胞嘧啶。“，”用于检测DNA中的羟甲基化胞嘧啶的试剂盒，其包含包含上述多价金属氧化物或多价金属酸盐和上述过氧化物的试剂。

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