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1. 发明授权

US07611849B2 Enhanced cellular assay method for use in flow cytometry or similar instruments using optically resonant particles 失效
标题翻译：用于流式细胞术或使用光学共振颗粒的类似仪器的增强的细胞测定方法
公开(公告)号：US07611849B2
公开(公告)日：2009-11-03
申请号：US10856284
申请日：2004-05-27
申请人： W. P. Hansen , Petra B. Krauledat
发明人： W. P. Hansen , Petra B. Krauledat
IPC分类号： G01N21/00 , G01N33/53 , G01N33/543 , G01N33/553 , G01N33/563
CPC分类号： G01N33/585 , G01N15/1427 , G01N15/1456 , G01N15/1459 , G01N2015/008 , G01N2015/1486
摘要： This invention relates to the field of biological assays where cells can be classified and enumerated using flow cytometry optical instrumentation. The invention combines information from multi-angle, light scatter from the cell itself and multi-angle light scatter from small, optically resonant particles that are selectively bound to surface molecules on the cell to carry out classification and enumeration. This light scatter method enables an instrumentation system that is simple to use, inexpensive to build, and mechanically robust; making it suitable for use in remote clinical environments.
摘要翻译：本发明涉及使用流式细胞术光学仪器对细胞进行分类和列举的生物测定领域。本发明结合了来自多角度的信息，来自细胞本身的光散射和来自小的光学共振颗粒的多角度光散射，其被选择性地结合到细胞上的表面分子以进行分类和计数。这种光散射方法使得能够简单使用，廉价构建和机械稳健的仪器系统; 使其适用于远程临床环境。

2. 发明授权

US5939021A Homogeneous binding assay 失效
标题翻译：均质结合试验
公开(公告)号：US5939021A
公开(公告)日：1999-08-17
申请号：US789211
申请日：1997-01-23
申请人： W. Peter Hansen , Petra B. Krauledat
发明人： W. Peter Hansen , Petra B. Krauledat
IPC分类号： C12Q1/68 , G01N33/543 , G01N33/58 , G01N21/00 , A01N1/02 , C12M1/00
CPC分类号： C12Q1/6816 , G01N33/54313 , G01N33/585 , Y10S435/975 , Y10S436/805 , Y10S436/808
摘要： A homogeneous method of measuring chemical binding relies on resonant, or "amplified," optical extinction (light scattering plus absorption) from a defined, specific class of colloidal particles wherein the real term n of the complex refractive index n-ik approaches zero while the imaginary term k approaches .sqroot.2. Chemical binding partners are coated onto the particles, which either aggregate or disperse during the binding reaction, causing an optical extinction change at one wavelength that is quantitatively related to the number of single colloidal particles and another at a second wavelength that is quantitatively related to the number of doublet colloidal particles. The method employs: (1) a specific class of colloidal particles that exhibit optical resonance and that are substantially smaller in diameter than a wavelength of visible light; (2) a one-step process of colloidal particle aggregation; and (3) a photometric extinction measurement at wavelengths where the particles exhibit optical resonance.
摘要翻译：测量化学结合的均匀方法依赖于来自限定的特定类型的胶体颗粒的共振或“放大”光学消光（光散射加吸收），其中复折射率n-ik的实数n接近零，而假想项k接近2ROOT + E，rad 2 + EE。将化学结合配偶体涂覆到颗粒上，颗粒在结合反应期间聚集或分散，导致一定波长的光学消光变化，其与单个胶体颗粒的数量定量相关，另一个在与第二个波长定量相关的另一个波长处双胶体颗粒数。该方法采用：（1）特定类型的胶体颗粒，其表现出光学共振，并且其直径小于可见光的波长; （2）胶体颗粒聚集的一步法; 和（3）在颗粒表现出光学共振的波长处的光度消光测量。

3. 发明授权

US07116407B2 System for axial pattern analysis of multicellular organisms 有权
公开(公告)号：US07116407B2
公开(公告)日：2006-10-03
申请号：US10076363
申请日：2002-02-15
申请人： W. Peter Hansen , Anthony A. Ferrante , Russell J. Gershman , Petra B. Krauledat , Donald F. Perrault, Jr.
发明人： W. Peter Hansen , Anthony A. Ferrante , Russell J. Gershman , Petra B. Krauledat , Donald F. Perrault, Jr.
IPC分类号： G01N21/00 , G01N33/553 , B07C5/36
CPC分类号： G01N33/5085 , G01N15/1427 , G01N15/1429 , G01N15/147 , G01N15/1475 , G01N2015/145 , G01N2015/1477 , G01N2015/149 , G01N2015/1497
摘要： A method of using elongate multicellular organisms in conjunction with a specialized flow cytometer for drug discovery and compound screening. A stable, optically detectable linear marker pattern on each organism is used to construct a longitudinal map of each organism as it passes through the analysis region of the flow cytometer. This pattern is used to limit complex data analysis to particular regions of each organism thereby simplifying and speeding analysis. The longitudinal marker pattern can be used to alter signal detection modes at known regions of the organism to enhance sensitivity and overall detection effectiveness. A repeating pattern can also be used to add a synchronous element to data analysis. The marker patterns are established using known methods of molecular biology to express various indicator molecules. Inherent features of the organism can be rendered detectable to serve as marker patterns.

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