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1. 发明申请

US20100330667A1 CHIMERIC GFP-AEQUORIN AS BIOLUMINESCENT CA++ REPORTERS AT THE SINGLE CELL LEVEL 审中-公开
标题翻译： CHEMERIC GFP-AEQUORIN作为BIOLUMESCENT CA ++报告器在单细胞水平
公开(公告)号：US20100330667A1
公开(公告)日：2010-12-30
申请号：US12794147
申请日：2010-06-04
申请人： Valérie BAUBET , Hervé Le Mouellic , Philippe Brulet
发明人： Valérie BAUBET , Hervé Le Mouellic , Philippe Brulet
IPC分类号： C12N15/63 , C07K14/435 , C07H21/04 , C07K7/06 , C07K7/08 , C07K14/00
CPC分类号： C07K7/06 , A61K49/0013 , C07K7/08 , C07K14/001 , C07K14/43595 , C07K2319/00 , Y10S435/968 , Y10S930/31
摘要： A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer (CRET).
摘要翻译：包含与光蛋白共价连接的荧光分子的修饰的生物发光体系，其中所述两种蛋白质之间的连接具有稳定修饰的生物发光体系并通过化学发光共振能量转移（CRET）传递能量的功能。

2. 发明授权

US07763443B2 Polynucleotides encoding chimeric GFP-aequorin as bioluminescent Ca++ reporters 失效
标题翻译：编码嵌合GFP-aequorin的多核苷酸作为生物发光Ca ++记者
公开(公告)号：US07763443B2
公开(公告)日：2010-07-27
申请号：US11149177
申请日：2005-06-10
申请人： Valérie Baubet , Hervé Le Mouellic , Philippe Brulet
发明人： Valérie Baubet , Hervé Le Mouellic , Philippe Brulet
IPC分类号： C12P21/06
CPC分类号： C07K7/06 , A61K49/0013 , C07K7/08 , C07K14/001 , C07K14/43595 , C07K2319/00 , Y10S435/968 , Y10S930/31
摘要： A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer(CRET).
摘要翻译：包含与光蛋白共价连接的荧光分子的修饰的生物发光体系，其中所述两种蛋白质之间的连接具有稳定修饰的生物发光体系并通过化学发光共振能量转移（CRET）传递能量的功能。

3. 发明授权

US06936475B2 Chimeric GFP-aequorin as bioluminescent Ca++reporters at the single cell level 失效
标题翻译：在单细胞水平嵌合GFP-aequorin作为生物发光的Ca ++记者
公开(公告)号：US06936475B2
公开(公告)日：2005-08-30
申请号：US10307389
申请日：2002-12-02
申请人： Valérie Baubet , Hervé Le Mouellic , Philippe Brulet
发明人： Valérie Baubet , Hervé Le Mouellic , Philippe Brulet
IPC分类号： G01N33/483 , A61K49/00 , C07K7/06 , C07K7/08 , C07K14/00 , C07K14/435 , C07K19/00 , C12N1/21 , C12N15/09 , C12Q1/02 , G01N21/76 , G01N33/84 , G01N33/52
CPC分类号： C07K7/06 , A61K49/0013 , C07K7/08 , C07K14/001 , C07K14/43595 , C07K2319/00 , Y10S435/968 , Y10S930/31
摘要： A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer (CRET).
摘要翻译：包含与光蛋白共价连接的荧光分子的修饰的生物发光体系，其中所述两种蛋白质之间的连接具有稳定修饰的生物发光体系并通过化学发光共振能量转移（CRET）传递能量的功能。

4. 发明授权

US06638768B1 Procedure for specific replacement of a copy of a gene present in the recipient genome by the integration of a gene different from that where the integration is made 失效
标题翻译：具体替换存在于受体基因组中的基因的拷贝通过整合不同于进行整合的基因的基因的程序
公开(公告)号：US06638768B1
公开(公告)日：2003-10-28
申请号：US08466699
申请日：1995-06-06
申请人： Hervé Le Mouellic , Philippe Brulet
发明人： Hervé Le Mouellic , Philippe Brulet
IPC分类号： C12N516
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/105 , A61K38/00 , A61K48/00 , C07K14/47 , C12N15/85 , C12N15/8509 , C12N15/907 , C12N2800/108 , C12N2800/30 , C12N2830/00 , C12N2830/85
摘要： The present invention relates to a process for targeted replacement of at least a part of an endogenous gene by at least a part of a foreign gene or targeted insertion of at least a part of a foreign gene at a targeted site in an endogenous gene in a cell by homologous recombination. This process includes (A) providing a vector which contains (1) at least a part of the foreign gene which is heterologous with respect to the endogenous gene, (2) a first flanking DNA sequence homologous to a first genomic sequence situated on one side of the part of the endogenous gene to be replaced or the targeted site; and (3) a second flanking DNA sequence homologous to a second genomic sequence situated on the other side of the part of the endogenous gene to be replaced or the targeted site, the foreign gene being located between the first and second flanking DNA sequences and is complementary to the part of the endogenous gene to be replaced; (B) transfecting a cell with the vector; (C) and selecting a transfected cell that contains the foreign gene at the targeted site or where the part of the endogenous gene is to be replaced. This invention also pertains to the vector, and the cell transfected by the vector.
摘要翻译：本发明涉及通过外源基因的至少一部分靶向置换至少一部分内源基因或至少部分外源基因靶向插入外源基因的内源基因的方法细胞通过同源重组。该方法包括（A）提供载体，该载体含有（1）相对于内源基因异源的外源基因的至少一部分，（2）与位于一侧的第一基因组序列同源的第一侧翼DNA序列的待更换的内源性基因部分或靶位点; 和（3）与位于待替代的内源基因部分的另一侧的第二基因组序列同源的第二侧翼DNA序列或靶位点，外源基因位于第一和第二侧翼DNA序列之间，并且是与待更换的内源基因的部分互补; （B）用载体转染细胞; （C），并且在靶位点选择含有外源基因的转染细胞，或者内源基因的一部分被置换的转染细胞。本发明还涉及载体和载体转染的细胞。

5. 发明授权

US06528313B1 Procedure for specific replacement of a copy of a gene present in the recipient genome by the integration of a gene different from that where the integration is made 失效
标题翻译：具体替换存在于受体基因组中的基因的拷贝通过整合不同于进行整合的基因的基因的程序
公开(公告)号：US06528313B1
公开(公告)日：2003-03-04
申请号：US08301037
申请日：1994-09-06
申请人： Hervé Le Mouellic , Philippe Brulet
发明人： Hervé Le Mouellic , Philippe Brulet
IPC分类号： C12N1564
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/105 , A61K38/00 , A61K48/00 , C07K14/47 , C12N15/85 , C12N15/8509 , C12N15/907 , C12N2800/108 , C12N2800/30 , C12N2830/00 , C12N2830/85
摘要： The present invention relates to a process for targeted replacement of at least a part of an endogenous gene by at least a part of a foreign gene or targeted insertion of at least a part of a foreign gene at a targeted site in an endogenous gene in a cell by homologous recombination. This process includes (A) providing a vector which contains (1) at least a part of the foreign gene which is heterologous with respect to the endogenous gene; (2) a first flanking DNA sequence homologous to a first genomic sequence situated on one side of the part of the endogenous gene to be replaced or the targeted site; and (3) a second flanking DNA sequence homologous to a second genomic sequence situated on the other side of the part of the endogenous gene to be replaced or the targeted site, the foreign gene being located between the first and second flanking DNA sequences and is complementary to the part of the endogenous gene to be replaced; (B) transfecting a cell with the vector; (C) and selecting a transfected cell that contains the foreign gene at the targeted site or where the part of the endogenous gene is to be replaced.
摘要翻译：本发明涉及通过外源基因的至少一部分靶向置换至少一部分内源基因或至少部分外源基因靶向插入外源基因的内源基因的方法细胞通过同源重组。该方法包括（A）提供载体，其含有（1）外源基因的至少一部分相对于内源基因是异源的; （2）与位于待置换的内源基因的一部分的一侧上的第一基因组序列或靶位点同源的第一侧翼DNA序列; 和（3）与位于待替代的内源基因部分的另一侧的第二基因组序列同源的第二侧翼DNA序列或靶位点，外源基因位于第一和第二侧翼DNA序列之间，并且是与待更换的内源基因的部分互补; （B）用载体转染细胞; （C），并且在靶位点选择含有外源基因的转染细胞，或者内源基因的一部分被置换的转染细胞。

6. 发明授权

US06528314B1 Procedure for specific replacement of a copy of a gene present in the recipient genome by the integration of a gene different from that where the integration is made 失效
标题翻译：具体替换存在于受体基因组中的基因的拷贝通过整合不同于进行整合的基因的基因的程序
公开(公告)号：US06528314B1
公开(公告)日：2003-03-04
申请号：US08466539
申请日：1995-06-06
申请人： Hervé Le Mouellic , Philippe Brulet
发明人： Hervé Le Mouellic , Philippe Brulet
IPC分类号： C12N1564
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/105 , A61K38/00 , A61K48/00 , C07K14/47 , C12N15/85 , C12N15/8509 , C12N15/907 , C12N2800/108 , C12N2800/30 , C12N2830/00 , C12N2830/85
摘要： A process for providing a recombinant, heterologous gene in the genome of a eukaryotic cell is provided. In particular, heterologous DNA is inserted into a recipient gene of the eukaryotic cell by homologous recombination. Moreover, a transgenic or chimeric animal comprising cells with DNA inserted into its genome by homologous recombination is disclosed. Further, the method of making these transgenic animal is taught.
摘要翻译：提供了在真核细胞的基因组中提供重组异源基因的方法。特别地，通过同源重组将异源DNA插入真核细胞的受体基因。此外，公开了包含通过同源重组插入到其基因组中的DNA的细胞的转基因或嵌合动物。此外，教导了制备这些转基因动物的方法。

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