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1. 发明申请

US20130123115A1 OPTIMIZED CELLULASE ENZYMES 有权
标题翻译：优化的纤维素酶
公开(公告)号：US20130123115A1
公开(公告)日：2013-05-16
申请号：US13578291
申请日：2011-02-11
申请人： Ulrich Kettling , Christoph Reisinger , Thomas Brück , Andre Koltermann , Jochen Gerlach , Isabel Unterstrasser , Lutz Röcher , Markus Rarbach , Jörg Claren , Andreas Kohl , Jan Carsten Pieck , Dominik Schlosser
发明人： Ulrich Kettling , Christoph Reisinger , Thomas Brück , Andre Koltermann , Jochen Gerlach , Isabel Unterstrasser , Lutz Röcher , Markus Rarbach , Jörg Claren , Andreas Kohl , Jan Carsten Pieck , Dominik Schlosser
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/24 , C12Y302/01091
摘要： The invention discloses cellulase enzymes with optimized properties for processing of cellulose- and lignocellulose-containing substrates. In particular, cellobiohydrolase enzymes with preferred characteristics are disclosed. The present invention provides fusion, insertion, deletion and/or substitution variants of such enzymes. Enzyme variants have enhanced thermostability, proteolytic stability, specific activity and/or stability at extreme pH. Nucleic acid molecules encoding said enzymes, a composition comprising said enzymes, a method for preparation, and the use for cellulose processing and/or for the production of biofuels are disclosed.
摘要翻译：本发明公开了具有优化的用于加工含纤维素和木质素纤维素的底物的纤维素酶。特别地，公开了具有优选特性的纤维二糖水解酶。本发明提供了这种酶的融合，插入，缺失和/或取代变体。酶变体在极端pH下具有增强的热稳定性，蛋白水解稳定性，比活度和/或稳定性。公开了编码所述酶的核酸分子，包含所述酶的组合物，制备方法以及用于纤维素加工和/或生产生物燃料的用途。

2. 发明授权

US09458443B2 Optimized cellulase enzymes 有权
标题翻译：优化的纤维素酶
公开(公告)号：US09458443B2
公开(公告)日：2016-10-04
申请号：US13578291
申请日：2011-02-11
申请人： Ulrich Kettling , Christoph Reisinger , Thomas Brück , Andre Koltermann , Jochen Gerlach , Isabel Unterstrasser , Lutz Röcher , Markus Rarbach , Jörg Claren , Andreas Kohl , Jan Carsten Pieck , Dominik Schlosser
发明人： Ulrich Kettling , Christoph Reisinger , Thomas Brück , Andre Koltermann , Jochen Gerlach , Isabel Unterstrasser , Lutz Röcher , Markus Rarbach , Jörg Claren , Andreas Kohl , Jan Carsten Pieck , Dominik Schlosser
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/24 , C12Y302/01091
摘要： The invention discloses cellulase enzymes with optimized properties for processing of cellulose- and lignocellulose-containing substrates. In particular, cellobiohydrolase enzymes with preferred characteristics are disclosed. The present invention provides fusion, insertion, deletion and/or substitution variants of such enzymes. Enzyme variants have enhanced thermostability, proteolytic stability, specific activity and/or stability at extreme pH. Nucleic acid molecules encoding said enzymes, a composition comprising said enzymes, a method for preparation, and the use for cellulose processing and/or for the production of biofuels are disclosed.
摘要翻译：本发明公开了具有优化的用于加工含纤维素和木质素纤维素的底物的纤维素酶。特别地，公开了具有优选特性的纤维二糖水解酶。本发明提供了这种酶的融合，插入，缺失和/或取代变体。酶变体在极端pH下具有增强的热稳定性，蛋白水解稳定性，比活性和/或稳定性。公开了编码所述酶的核酸分子，包含所述酶的组合物，制备方法以及用于纤维素加工和/或生产生物燃料的用途。

3. 发明授权

US08679795B2 Thermostable xylanase for the selective hydrolysis of pentose-containing polysaccharides 有权
标题翻译：用于选择性水解含糖的多糖的热稳定的木聚糖酶
公开(公告)号：US08679795B2
公开(公告)日：2014-03-25
申请号：US13517344
申请日：2010-12-15
申请人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
发明人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
IPC分类号： C12N9/24
CPC分类号： C12N9/248
摘要： The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicellulose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.
摘要翻译：本发明涉及具有木聚糖酶活性的多肽和编码此类多肽的核酸序列。本发明还涉及包含核酸构建体的核酸构建体，载体和宿主细胞以及用于产生和使用多肽的方法。木聚糖酶的一个具体应用是含有半纤维素的植物生物质的戊糖糖成分的选择性水解。核苷酸序列可用于生产木聚糖酶或其优化的突变体。

4. 发明申请

US20130109062A1 THERMOSTABLE XYLANASE FOR THE SELECTIVE HYDROLYSIS OF PENTOSE-CONTAINING POLYSACCHARIDES 有权
标题翻译：用于选择性水解含PENTOSE的多糖的热稳定性XYLANASE
公开(公告)号：US20130109062A1
公开(公告)日：2013-05-02
申请号：US13517344
申请日：2010-12-15
申请人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
发明人： Andreas Kohl , Isabel Unterstrasser , Markus Rarbach , Andre Koltermann , Christoph Reisinger , Thomas Brück , Ulrich Kettling , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson
IPC分类号： C12N9/24
CPC分类号： C12N9/248
摘要： The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicellulose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.
摘要翻译：本发明涉及具有木聚糖酶活性的多肽和编码此类多肽的核酸序列。本发明还涉及包含核酸构建体的核酸构建体，载体和宿主细胞以及用于产生和使用多肽的方法。木聚糖酶的一个具体应用是含有半纤维素的植物生物质的戊糖糖成分的选择性水解。核苷酸序列可用于生产木聚糖酶或其优化的突变体。

5. 发明申请

US20140045227A1 EFFICIENT LIGNOCELLULOSE HYDROLYSIS WITH INTEGRATED ENZYME PRODUCTION 审中-公开
标题翻译：具有综合酶生产的高效液体纤维素水解
公开(公告)号：US20140045227A1
公开(公告)日：2014-02-13
申请号：US13977428
申请日：2012-01-02
申请人： Markus Rarbach , Zdravko Dragovic , Andreas Kohl , Jochen Gerlach , Jörg Bartuch , Thomas Brück
发明人： Markus Rarbach , Zdravko Dragovic , Andreas Kohl , Jochen Gerlach , Jörg Bartuch , Thomas Brück
IPC分类号： C12P19/14
CPC分类号： C12P19/14 , C12N9/2434 , C12P7/10 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a process for degradation of lignocellulosic biomass, which has optionally been pre-treated. It is based on the finding that hydrolysis efficiency of the biomass is enhanced in the presence of a mechanically or chemically treated microorganism capable of producing the respective hydrolytic enzymes. The invention therefore provides a process for degradation of lignocellulosic biomass with integrated produced enzyme cocktails. The invention also provides a process wherein the part of the optionally pre-treated lignocellulosic biomass is incorporated into the final growth medium of the fungus.
摘要翻译：本发明提供了一种木质纤维素生物质的降解方法，其中任选地已经预处理过。基于这样的发现，即在存在能够产生相应的水解酶的机械或化学处理的微生物的情况下，生物质的水解效率得到提高。因此，本发明提供了一种用综合生产的酶混合物降解木质纤维素生物质的方法。本发明还提供了一种方法，其中任选预处理的木质纤维素生物质的一部分并入真菌的最终生长培养基中。

6. 发明授权

US11898275B2 Carbon fibers which can be produced regeneratively or part-regeneratively from CO2 using combined production methods 有权
公开(公告)号：US11898275B2
公开(公告)日：2024-02-13
申请号：US17487503
申请日：2021-09-28
申请人： Kolja Kuse , Uwe Arnold , Thomas Brück
发明人： Kolja Kuse , Uwe Arnold , Thomas Brück
IPC分类号： D01F9/22 , C08F220/44 , C08F120/44 , C12P7/6445
CPC分类号： D01F9/22 , C08F120/44 , C08F220/44 , C12P7/6445
摘要： The invention describes carbon fibers which are produced on the basis of different process chains from CO2. These include routes through natural resources such as algal biomass to produce carbon fiber precursors such as PAN from CO2, as well as the purely synthetic route via the Fischer-Tropsch synthesis, which is also used to make CO2 carbon fiber precursors. In this way, CO2 from anthropogenic origin is to be converted into a solid aggregate state of carbon fiber, which can be disposed of at the end of its life cycle, after being used as highly valuable building material for industry and man, for the construction of buildings and vehicles. These processes produce by-products such as biodiesel and nutrients that generate added value. The production volumes of the resulting substances should be controllable by combining the methods presented here. Some of these processes alone have no long-term climate relevance because of the high costs, but in the initial phase of such a development with the help of carbon dioxide certificates or socio-political necessities they are able to quickly show that carbon fiber building materials can be produced which by themselves are made from CO2 and at least have the quality to be used in the construction sector and for example are feasible to replace steel, in that the paradigm of todays material production being CO2-positive, can be turned into the opposite. If the processes—which have the disadvantage of large-area consumption on the one hand and the of the lack of energy efficiency in the longer term on the other—can be coupled, they have the potential to support each other. By combining the methods, land use and costs can be adjusted to current regional economic performance based on the material paradigm of the future of carbon-negative production of carbon fibers, also depending on the current evolution of CO2 emission allowance prices. The invention has the desired effect in climate policy that high-tech technology transfer can take place into the currently disadvantaged regions of the world, which promotes the economic performance of today's disadvantaged regions and in particular creates the urgently needed jobs in these regions.

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