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1. 发明申请

US20090233280A1 METHOD OF ACQUIRING INFORMATION REGARDING BASE SEQUENCE AND INFORMATION READING DEVICE FOR THE SAME 审中-公开
标题翻译：获取基本序列信息的方法和相关信息读取装置
公开(公告)号：US20090233280A1
公开(公告)日：2009-09-17
申请号：US12093138
申请日：2006-12-26
申请人： Tsuyoshi Nomoto , Wataru Kubo
发明人： Tsuyoshi Nomoto , Wataru Kubo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B82Y30/00 , C12Q2563/113 , C12Q2537/149 , C12Q2533/101 , C12Q2535/125 , C12Q2525/186
摘要： Information regarding a base sequence of a target nucleic acid is acquired by preparing a sample comprising the target nucleic acid hybridized with a primer or a sample comprising the target nucleic acid containing a promoter sequence, a polymerase, a nucleotide derivative having an electrochemically convertible moiety; allowing the three components to coexist in a solvent; and detecting whether the nucleotide derivative is introduced into the primer or a transcription product of the target nucleic acid or not using an electrochemical reaction.
摘要翻译：通过制备包含与引物或包含含有启动子序列的靶核酸的样品，聚合酶，具有电化学转换部分的核苷酸衍生物杂交的靶核酸的样品获得关于靶核酸的碱基序列的信息; 使三种成分共存于溶剂中; 以及使用电化学反应来检测是否将核苷酸衍生物引入引物或靶核酸的转录产物。

2. 发明授权

US07632600B2 Enzyme electrode and method of producing the same 失效
标题翻译：酶电极及其制造方法
公开(公告)号：US07632600B2
公开(公告)日：2009-12-15
申请号：US11525848
申请日：2006-09-25
申请人： Wataru Kubo , Tsuyoshi Nomoto , Tetsuya Yano , Rie Ueno
发明人： Wataru Kubo , Tsuyoshi Nomoto , Tetsuya Yano , Rie Ueno
IPC分类号： H01M4/00 , H01M4/88 , C12N11/00
CPC分类号： H01M8/16 , H01M4/8803 , H01M4/8817 , H01M4/8828 , H01M4/8882 , H01M4/9008 , Y02E60/527
摘要： An enzyme electrode that enables to enhance the rate of charge transfer from a redox center of an enzyme and catalytic current is provided. An enzyme electrode capable of increasing catalytic current by increasing the rate of charge transfer from an enzyme using an enzyme/metal fine particle complex in which part of a metal fine particle is incorporated into the enzyme can be provided.
摘要翻译：提供能够提高从酶的氧化还原中心的电荷转移速率和催化电流的酶电极。可以提供能够通过增加使用其中部分金属微粒掺入到酶中的酶/金属微粒络合物从酶的电荷转移速率来增加催化电流的酶电极。

3. 发明申请

US20070190590A1 Information acquisition apparatus on concentration of thioredoxins in sample, stress level information acquisition apparatus and stress level judging method 审中-公开
标题翻译：样品中硫氧还蛋白浓度信息获取装置，应力水平信息获取装置和应力水平评估方法
公开(公告)号：US20070190590A1
公开(公告)日：2007-08-16
申请号：US11700853
申请日：2007-02-01
申请人： Wataru Kubo , Tsuyoshi Nomoto
发明人： Wataru Kubo , Tsuyoshi Nomoto
IPC分类号： C12Q1/26 , G06G7/48
CPC分类号： C12Q1/008 , C12Q1/26 , G01N33/573 , G01N33/6893 , G01N2800/30
摘要： The invention is to provide an information acquisition apparatus for acquiring information relating to at least one of an oxidized form concentration, a reduced form concentration and a ratio of the concentrations of thioredoxin, useful for judging a stress level, and a stress level information acquisition apparatus and a stress level judging method utilizing the same. A reaction by an enzyme or the like catalyzing an redox reaction of thioredoxins is used to measure at least one of an oxidized form concentration, a reduced form concentration and a concentration ratio of thioredoxins, and data of such measurement is used for judging the stress level of a subject person.
摘要翻译：本发明提供一种信息获取装置，用于获取关于用于判断应力水平的硫氧还蛋白浓度的氧化形式浓度，浓缩形式浓度和浓度之比以及应力水平信息获取装置中的至少一种的信息以及利用该判定方法的应力水平判断方法。催化硫氧还蛋白的氧化还原反应的酶等的反应用于测定氧化形式浓度，还原形式浓度和硫氧还蛋白浓度比中的至少一种，并且使用这种测量的数据来判断应力水平的主题人。

4. 发明申请

US20070131546A1 Enzyme electrode 审中-公开
标题翻译：酶电极
公开(公告)号：US20070131546A1
公开(公告)日：2007-06-14
申请号：US11634921
申请日：2006-12-07
申请人： Tsuyoshi Nomoto , Wataru Kubo , Tetsuya Yano
发明人： Tsuyoshi Nomoto , Wataru Kubo , Tetsuya Yano
IPC分类号： G01N33/487 , H01M4/90
CPC分类号： C12Q1/001 , C12Q1/32 , H01M4/9008 , H01M8/16 , Y02E60/527
摘要： There is provided with an enzyme electrode which can be used as a sensor with high sensitivity, a biofuel cell with high output, and an electrochemical reaction device with high reaction efficiency. The enzyme electrode has a conductive base plate, a fusion protein immobilized to the conductive base plate and an electron transfer mediator, wherein the fusion protein is a fusion protein of a enzyme 1 to catalyze a chemical reaction for producing a reaction product 1 from a reaction substrate 1 and a enzyme 2 to catalyze a chemical reaction for producing a reaction product 2 from a reaction substrate 2, and at least one chemical substance of the reaction product 1 is identical to at least one chemical substance of the reaction substrate 2.
摘要翻译：提供了可用作具有高灵敏度的传感器，高产量的生物燃料电池的酶电极和具有高反应效率的电化学反应装置。酶电极具有导电性基板，固定在导电性基板上的融合蛋白质和电子转移介质，其中融合蛋白是酶1的融合蛋白，用于催化从反应产生反应产物1的化学反应底物1和酶2以催化从反应基材2制备反应产物2的化学反应，并且反应产物1的至少一种化学物质与反应基底2的至少一种化学物质相同。

5. 发明申请

US20070056852A1 Enzyme electrode sensor fuel cell and electrochemical reactor 审中-公开
标题翻译：酶电极传感器燃料电池和电化学反应器
公开(公告)号：US20070056852A1
公开(公告)日：2007-03-15
申请号：US10573179
申请日：2005-07-22
申请人： Wataru Kubo , Tetsuya Yano , Tsuyoshi Nomoto
发明人： Wataru Kubo , Tetsuya Yano , Tsuyoshi Nomoto
IPC分类号： C12Q1/00 , H01M4/90
CPC分类号： C12Q1/004
摘要： An enzyme electrode comprises a conductive member and an enzyme, wherein a first mediator and a second mediator are immobilized by a carrier onto the conductive member, the first mediator and the second mediator having different redox potentials (reduction-oxidation potentials). A sensor employs the enzyme electrode as a detection portion for detecting a substance. A fuel cell employs the enzyme electrode as at least one of anode and a cathode. An electrochemical reactor employs the enzyme electrode as a reaction electrode.
摘要翻译：酶电极包括导电构件和酶，其中第一介体和第二介体通过载体固定在导电构件上，第一介体和第二介体具有不同的氧化还原电位（还原氧化电位）。传感器使用酶电极作为检测物质的检测部。燃料电池使用酶电极作为阳极和阴极中的至少一个。电化学反应器采用酶电极作为反应电极。

6. 发明申请

US20120325680A1 INFORMATION ACQUISITION APPARATUS ON CONCENTRATION OF THIOREDOXINS IN SAMPLE, STRESS LEVEL INFORMATION ACQUISITION APPARATUS AND STRESS LEVEL JUDGING METHOD 审中-公开
标题翻译：信息获取装置在样品中应用三氧化二砷，应力水平信息获取装置和应力水平评估方法
公开(公告)号：US20120325680A1
公开(公告)日：2012-12-27
申请号：US13584695
申请日：2012-08-13
申请人： Wataru Kubo , Tsuyoshi Nomoto
发明人： Wataru Kubo , Tsuyoshi Nomoto
IPC分类号： G01N27/26
CPC分类号： C12Q1/008 , C12Q1/26 , G01N33/573 , G01N33/6893 , G01N2800/30
摘要： The invention is to provide an information acquisition apparatus for acquiring information relating to at least one of an oxidized form concentration, a reduced form concentration and a ratio of the concentrations of thioredoxin, useful for judging a stress level, and a stress level information acquisition apparatus and a stress level judging method utilizing the same. A reaction by an enzyme or the like catalyzing an redox reaction of thioredoxins is used to measure at least one of an oxidized form concentration, a reduced form concentration and a concentration ratio of thioredoxins, and data of such measurement is used for judging the stress level of a subject person.
摘要翻译：本发明提供一种信息获取装置，用于获取关于用于判断应力水平的硫氧还蛋白浓度的氧化形式浓度，浓缩形式浓度和浓度之比以及应力水平信息获取装置中的至少一种的信息以及利用该判定方法的应力水平判断方法。催化硫氧还蛋白的氧化还原反应的酶等的反应用于测定氧化形式浓度，还原形式浓度和硫氧还蛋白浓度比中的至少一种，并且使用这种测量的数据来判断应力水平的主题人。

7. 发明申请

US20080248354A1 Enzyme Electrode, and Device, Sensor, Fuel Cell and Electrochemical Reactor Employing the Enzyme Electrode 审中-公开
标题翻译：酶电极和装置，传感器，燃料电池和使用酶电极的电化学反应器
公开(公告)号：US20080248354A1
公开(公告)日：2008-10-09
申请号：US10571687
申请日：2005-07-22
申请人： Wataru Kubo , Tsuyoshi Nomoto , Tetsuya Yano
发明人： Wataru Kubo , Tsuyoshi Nomoto , Tetsuya Yano
IPC分类号： H01M8/02 , G01N27/26 , B05D5/12 , H01M4/02
CPC分类号： C12Q1/004 , H01M8/16 , Y02E60/527 , Y02P70/56
摘要： An enzyme electrode has a conductive member and an enzyme, wherein the conductive member has a porous structure, and the enzyme is immobilized through a carrier in pores constituting the porous structure. An enzyme electrode device, comprises the enzyme electrode, and wiring connected to the conductive member of the enzyme electrode.
摘要翻译：酶电极具有导电构件和酶，其中导电构件具有多孔结构，并且酶通过载体固定在构成多孔结构的孔中。酶电极装置，包括酶电极和连接到酶电极的导电构件的配线。

8. 发明申请

US20080102323A1 ENZYME ELECTRODE, ENZYME ELECTRODE PRODUCING METHOD, SENSOR AND FUEL CELL EACH USING ENZYME ELECTRODE 有权
标题翻译： ENZYME ELECTRODE，ENZYME ELECTRODE PRODUCTION METHOD，SENSOR AND FUEL CELL EACH USING ENZYME ELECTRODE
公开(公告)号：US20080102323A1
公开(公告)日：2008-05-01
申请号：US11837074
申请日：2007-08-10
申请人： Wataru Kubo , Tsuyoshi Nomoto
发明人： Wataru Kubo , Tsuyoshi Nomoto
IPC分类号： C12M1/40 , H01M8/02 , C12P21/04 , H01M4/00
CPC分类号： C12Q1/001 , H01M4/90 , H01M8/16 , Y02E60/527 , Y02P70/56
摘要： An amino acid capable of coordinately binding to a metallic complex is introduced to an active site within the enzyme or another controlled position near the active site. Therefore, when a mediator is introduced into the enzyme, a mediator-introduced position is controlled to be held at or near the active site.
摘要翻译：能够与金属配合物配位结合的氨基酸被引入活性位点附近的酶或其他控制位置的活性位点。因此，当将介体引入酶时，介导介质位置被控制为保持在活性位点处或附近。

9. 发明申请

US20070131547A1 Enzyme electrode 审中-公开
标题翻译：酶电极
公开(公告)号：US20070131547A1
公开(公告)日：2007-06-14
申请号：US11637782
申请日：2006-12-13
申请人： Tsuyoshi Nomoto , Wataru Kubo , Tetsuya Yano
发明人： Tsuyoshi Nomoto , Wataru Kubo , Tetsuya Yano
IPC分类号： G01N33/487 , H01M4/90
CPC分类号： C12Q1/32 , C12Q1/001 , H01M4/9008 , H01M8/16 , Y02E60/527
摘要： The invention provides an enzyme electrode usable as a highly sensitive sensor, a high-output bio fuel cell or an electrochemical reaction device of a high reaction efficiency. The enzyme electrode includes an electroconductive base member and an enzyme, wherein the enzyme is formed by an associated protein in which two or more different enzyme proteins are associated.
摘要翻译：本发明提供可用作高灵敏度传感器，高输出生物燃料电池或反应效率高的电化学反应装置的酶电极。酶电极包括导电性基材和酶，其中酶由其中两种或更多种不同酶蛋白缔合的相关蛋白形成。

10. 发明申请

US20070077483A1 Enzyme electrode and method of producing the same 失效
标题翻译：酶电极及其制造方法
公开(公告)号：US20070077483A1
公开(公告)日：2007-04-05
申请号：US11525848
申请日：2006-09-25
申请人： Wataru Kubo , Tsuyoshi Nomoto , Tetsuya Yano , Rie Ueno
发明人： Wataru Kubo , Tsuyoshi Nomoto , Tetsuya Yano , Rie Ueno
IPC分类号： H01M4/90 , H01M4/88
CPC分类号： H01M8/16 , H01M4/8803 , H01M4/8817 , H01M4/8828 , H01M4/8882 , H01M4/9008 , Y02E60/527
摘要： An enzyme electrode that enables to enhance the rate of charge transfer from a redox center of an enzyme and catalytic current is provided. An enzyme electrode capable of increasing catalytic current by increasing the rate of charge transfer from an enzyme using an enzyme/metal fine particle complex in which part of a metal fine particle is incorporated into the enzyme can be provided.
摘要翻译：提供能够提高从酶的氧化还原中心的电荷转移速率和催化电流的酶电极。可以提供能够通过增加使用其中部分金属微粒掺入到酶中的酶/金属微粒络合物从酶的电荷转移速率来增加催化电流的酶电极。

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