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1. 发明授权

US06723562B1 DNA construct for transforming a yeast 有权
标题翻译：用于转化酵母的DNA构建体
公开(公告)号：US06723562B1
公开(公告)日：2004-04-20
申请号：US09869185
申请日：2001-06-25
申请人： Toshihiko Ashikari , Misa Ochiai
发明人： Toshihiko Ashikari , Misa Ochiai
IPC分类号： C12N1564
CPC分类号： C12C12/004 , C12C12/006 , C12N15/81 , C12R1/85
摘要： A DNA construct comprising: (1) a selective marker gene, (2) a galactose-inducible growth inhibition sequence, (3) a pair of FRT sequences in the same orientation flanking (1) and (2), and (4) a DNA fragment capable of recombining with a yeast chromosomal DNA located at each end of (3), wherein said FRT sequences contain the following sequence: 5′-GAAGTTCCTATAC TTTCTAGA GAATAGGAACTTC-3′ (SEQ ID NO: 1) inverted spacer inverted repeat (1) sequence repeat (2) or a sequence substantially identical to said sequence.
摘要翻译：一种DNA构建体，包括：（1）选择性标记基因，（2）半乳糖诱导型生长抑制序列，（3）在侧翼（1）和（2）中具有相同方向的一对FRT序列，和（4）a 能够与位于（3）的每个端部的酵母染色体DNA重组的DNA片段，其中所述FRT序列包含以下序列：5'-GAAGTTCCTATAC TTTCTAGA GAATAGGAACTTC-3'（SEQ ID NO：1）或与说序列。

2. 发明授权

US09447393B2 Phosphatidic acid phosphatase gene 有权
标题翻译：磷脂酸性磷酸酶基因
公开(公告)号：US09447393B2
公开(公告)日：2016-09-20
申请号：US14235146
申请日：2012-07-27
申请人： Misa Ochiai
发明人： Misa Ochiai
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , C12Y301/03004
摘要： The present invention provides a novel phosphatidic acid phosphatase gene. The object of the present invention can be solved by providing a nucleic acid comprising the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 4, or SEQ ID NO: 5; a protein comprising the amino acid sequence set forth in SEQ ID NO: 2; and mutants thereof.
摘要翻译：本发明提供了一种新的磷脂酸磷酸酶基因。本发明的目的可以通过提供包含SEQ ID NO：1，SEQ ID NO：4或SEQ ID NO：5所示的核苷酸序列的核酸来解决。包含SEQ ID NO：2所示的氨基酸序列的蛋白质; 及其突变体。

3. 发明授权

US09051590B2 Acetyl-CoA carboxylase 有权
标题翻译：乙酰CoA羧化酶
公开(公告)号：US09051590B2
公开(公告)日：2015-06-09
申请号：US13254512
申请日：2010-03-17
申请人： Misa Ochiai , Eiji Sakuradani , Sakayu Shimizu , Jun Ogawa
发明人： Misa Ochiai , Eiji Sakuradani , Sakayu Shimizu , Jun Ogawa
IPC分类号： C07H21/02 , C07H21/04 , C12Q1/68 , C12P21/06 , C12P21/04 , C12N15/00 , C12P7/64 , A23L1/30 , C12N9/00
CPC分类号： C12P7/6427 , A23L33/115 , A23V2002/00 , C12N9/93 , C12Y604/01002 , A23V2250/1882
摘要： The present invention provides a novel acetyl-CoA carboxylase.The object of the present invention is attained by the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 of the present invention.
摘要翻译：本发明提供了一种新的乙酰辅酶A羧化酶。本发明的目的是通过本发明的SEQ ID NO：1的核苷酸序列和SEQ ID NO：2的氨基酸序列来实现的。

4. 发明申请

US20140234941A1 PHOSPHATIDIC ACID PHOSPHATASE GENE 有权
标题翻译：磷酸磷酸酯酶基因
公开(公告)号：US20140234941A1
公开(公告)日：2014-08-21
申请号：US14235146
申请日：2012-07-27
申请人： Misa Ochiai
发明人： Misa Ochiai
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , C12Y301/03004
摘要： The present invention provides a novel phosphatidic acid phosphatase gene. The object of the present invention can be solved by providing a nucleic acid comprising the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 4, or SEQ ID NO: 5; a protein comprising the amino acid sequence set forth in SEQ ID NO: 2; and mutants thereof.
摘要翻译：本发明提供了一种新的磷脂酸磷酸酶基因。本发明的目的可以通过提供包含SEQ ID NO：1，SEQ ID NO：4或SEQ ID NO：5所示的核苷酸序列的核酸来解决。包含SEQ ID NO：2所示的氨基酸序列的蛋白质; 及其突变体。

5. 发明授权

US08765423B2 Diacylglycerol acyltransferase genes and use thereof 有权
标题翻译：二酰基甘油酰基转移酶基因及其用途
公开(公告)号：US08765423B2
公开(公告)日：2014-07-01
申请号：US13512109
申请日：2010-12-20
申请人： Misa Ochiai
发明人： Misa Ochiai
IPC分类号： C12N9/10 , C12N15/63 , C07H21/04
CPC分类号： C12N9/1029 , A23L33/115 , A61K8/361 , A61K8/375 , A61K8/606 , A61K8/66 , A61K31/202 , A61K31/232 , A61Q19/00 , C12P7/6463 , C12P7/6472 , C12Y203/0102
摘要： It is an object to provide a novel diacylglycerol acyltransferase. The present invention relates to a diacylglycerol acyltransferase, a polynucleotide encoding the same, and so on. The present invention provides a polynucleotide comprising the nucleotide sequence of, e.g., SEQ ID NO: 1 or 4, a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2, an expression vector and transformant comprising the polynucleotide, a method for producing a lipid or fatty acid composition using the transformant, or a food, etc. comprising the lipid or fatty acid produced by the method.
摘要翻译：本发明的目的是提供一种新的二酰基甘油酰基转移酶。本发明涉及二酰基甘油酰基转移酶，编码其的多核苷酸等等。本发明提供了包含例如SEQ ID NO：1或4的核苷酸序列的多核苷酸，编码由SEQ ID NO：2的氨基酸序列组成的蛋白质的多核苷酸，表达载体和包含多核苷酸的转化体，使用该转化体生产脂质或脂肪酸组合物的方法，或包含通过该方法生产的脂质或脂肪酸的食品等。

6. 发明申请

US20130252308A1 GLYCEROL-3-PHOSPHATE ACYL TRANSFERASE 有权
标题翻译：甘油-3-磷酸酯转移酶
公开(公告)号：US20130252308A1
公开(公告)日：2013-09-26
申请号：US13496081
申请日：2010-09-21
申请人： Misa Ochiai
发明人： Misa Ochiai
IPC分类号： C12N9/10
CPC分类号： C12N9/1029 , A23D7/00 , A23D9/00 , A23L7/00 , A23L31/00 , A23L31/10 , A23L33/115 , A61K8/361 , A61K8/99 , A61Q19/00 , C11B1/00 , C12P7/6409 , C12P7/6463 , C12Y203/01015
摘要： The present invention relates to glycerol-3-phosphate acyltransferases, polynucleotides encoding the same, etc. The present invention provides a polynucleotide comprising the nucleotide sequence of e.g., SEQ ID NO: 1 or 4, a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2, an expression vector and transformant comprising the polynucleotide, a method for producing food, etc. using the transformant, food, etc. produced by the method, and so on.
摘要翻译：本发明涉及甘油-3-磷酸酰基转移酶，编码其的多核苷酸等。本发明提供了包含例如SEQ ID NO：1或4的核苷酸序列的多核苷酸，编码由氨基酸组成的蛋白质的多核苷酸 SEQ ID NO：2的序列，表达载体和包含多核苷酸的转化体，使用通过该方法生产的转化体，食品等的食品的制造方法等。

7. 发明申请

US20120309950A1 PHOSPHATIDIC ACID PHOSPHATASE GENE AND USE THEREOF 有权
标题翻译：磷酸磷酸酯酶基因及其用途
公开(公告)号：US20120309950A1
公开(公告)日：2012-12-06
申请号：US13518087
申请日：2010-12-27
申请人： Misa Ochiai
发明人： Misa Ochiai
IPC分类号： C07H21/04 , C12N9/16 , C12N5/10 , C07F9/02 , C12N1/15 , C12N1/19 , C12N15/63 , C12P7/64 , C12N1/21
CPC分类号： C12N9/16 , C12N15/8247 , C12P7/6463 , C12Y301/03004 , Y02P20/52
摘要： The present invention provides a novel phosphatidic acid phosphatase gene. The object of the present invention can be solved by providing a nucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 7, SEQ ID NO: 4 or SEQ ID NO: 9, or SEQ ID NO: 5 or SEQ ID NO: 10; and an amino acid sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 6.
摘要翻译：本发明提供了一种新的磷脂酸磷酸酶基因。本发明的目的可以通过提供SEQ ID NO：1或SEQ ID NO：7，SEQ ID NO：4或SEQ ID NO：9或SEQ ID NO：5或SEQ ID NO： NO：10; 和SEQ ID NO：2或SEQ ID NO：6所示的氨基酸序列。

8. 发明申请

US20120264960A1 NOVEL ACETYL-CoA CARBOXYLASE 有权
标题翻译：新型乙酰辅酶A羧酸酶
公开(公告)号：US20120264960A1
公开(公告)日：2012-10-18
申请号：US13254512
申请日：2010-03-17
申请人： Misa Ochiai , Eiji Sakuradani , Sakayu Shimizu , Jun Ogawa
发明人： Misa Ochiai , Eiji Sakuradani , Sakayu Shimizu , Jun Ogawa
IPC分类号： C12N9/00 , C12N15/63 , C12P7/64 , C07C57/03 , C12N1/15 , C12N1/19 , C12N5/10 , C12P9/00 , C12N15/52 , C12N1/21
CPC分类号： C12P7/6427 , A23L33/115 , A23V2002/00 , C12N9/93 , C12Y604/01002 , A23V2250/1882
摘要： The present invention provides a novel acetyl-CoA carboxylase. The object of the present invention is attained by the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 of the present invention.
摘要翻译：本发明提供了一种新的乙酰辅酶A羧化酶。本发明的目的是通过本发明的SEQ ID NO：1的核苷酸序列和SEQ ID NO：2的氨基酸序列来实现的。

9. 发明授权

US08247209B2 Glycerol-3-phosphate acyltransferase (GPAT) homologs and use thereof 有权
标题翻译：甘油-3-磷酸酰基转移酶（GPAT）同系物及其用途
公开(公告)号：US08247209B2
公开(公告)日：2012-08-21
申请号：US12515834
申请日：2008-06-12
申请人： Misa Ochiai , Hisanori Tokuda
发明人： Misa Ochiai , Hisanori Tokuda
IPC分类号： C12N1/20 , C12N15/11
CPC分类号： C12P7/6409 , A21D8/042 , A23D9/00 , A23K20/189 , A23L33/12 , C12N9/1029 , C12P7/6427
摘要： The present invention provides novel genes for glycerol-3-phosphate acyltransferase. In exemplary embodiments, the invention provides a nucleic acid comprising the nucleotide sequence shown in SEQ ID NO: 1 or 4 or a fragment thereof.
摘要翻译：本发明提供了甘油-3-磷酸酰基转移酶的新基因。在示例性实施方案中，本发明提供了包含SEQ ID NO：1或4所示核苷酸序列或其片段的核酸。

10. 发明授权

US07927845B2 Fatty acid synthetase, polynucleotide encoding the same, and uses thereof 有权
标题翻译：脂肪酸合成酶，编码其的多核苷酸及其用途
公开(公告)号：US07927845B2
公开(公告)日：2011-04-19
申请号：US12299662
申请日：2007-05-08
申请人： Misa Ochiai
发明人： Misa Ochiai
IPC分类号： C12P7/64 , C12N1/00 , C07H21/04
CPC分类号： C12N9/1029 , C12P7/6409 , C12P7/6463
摘要： The present invention provides fatty acid synthetases which are responsible for novel fatty acid synthesis, polynucleotides which encode such fatty acid synthetases (e.g., a polynucleotide comprising (a) a polynucleotide consisting of the nucleotide sequence of Positions 1 to 12486 of SEQ ID NO: 1, or (b) a polynucleotide which hybridizes under stringent conditions to a polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence of Positions 1 to 12486 of SEQ ID NO: 1, and which encodes a protein having a fatty acid synthetase activity), expression vectors and transformants comprising such polynucleotides, methods for producing food and other products using such transformants, food products produced by such methods, and methods for assessing and selecting lipid-producing test fungi.
摘要翻译：本发明提供负责新型脂肪酸合成的脂肪酸合成酶，编码这种脂肪酸合成酶的多核苷酸（例如，包含（a）由SEQ ID NO：1的位置1至12486的核苷酸序列组成的多核苷酸的多核苷酸，或（b）在严格条件下与包含与SEQ ID NO：1的位置1至12486的核苷酸序列互补的核苷酸序列并且编码具有脂肪酸合成酶活性的蛋白质的核苷酸序列的多核苷酸杂交的多核苷酸），表达包含这种多核苷酸的载体和转化体，使用这种转化体生产食物和其它产品的方法，通过这些方法产生的食品，以及评估和选择脂质生产试验真菌的方法。

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