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1. 发明申请

US20090325256A1 METHOD FOR CELL PATTERNING 审中-公开
标题翻译：细胞方法
公开(公告)号：US20090325256A1
公开(公告)日：2009-12-31
申请号：US12310042
申请日：2007-08-03
申请人： Tomoyuki Yasukawa , Masato Suzuki , Hitoshi Shiku , Yoshio Hori , Akiko Inagaki , Mariko Komabayashi , Tomokazu Matsue
发明人： Tomoyuki Yasukawa , Masato Suzuki , Hitoshi Shiku , Yoshio Hori , Akiko Inagaki , Mariko Komabayashi , Tomokazu Matsue
IPC分类号： C12N13/00
CPC分类号： C12N5/0068 , C12M33/00 , C12N2535/10
摘要： Provided is a method for cell patterning, using an electrode substrate including a plurality of electrodes and a cell culture substrate disposed so as to face the electrode substrate, the method comprising the steps of: introducing a cell suspension containing cells into a region between the electrode substrate and the cell culture substrate; applying a voltage to the electrodes to generate a non-uniform electric field in the region; and arranging the cells at a position with low electric field strength on the cell culture substrate by utilizing negative dielectrophoresis so as to obtain the cell culture substrate on which the cells are arranged in a predetermined pattern.
摘要翻译：提供一种细胞图案化方法，使用包括多个电极的电极基板和与电极基板相对配置的细胞培养基板，该方法包括以下步骤：将含有细胞的细胞悬浮液导入到电极底物和细胞培养基质; 向电极施加电压以在该区域中产生不均匀的电场; 并且通过利用负介电电泳在细胞培养基板上将细胞排列在电场强度低的位置，以获得细胞以预定图案排列的细胞培养基质。

2. 发明授权

US5866321A Analytical method and device for precise analysis with a simple sensor 失效
标题翻译：用简单传感器精确分析的分析方法和装置
公开(公告)号：US5866321A
公开(公告)日：1999-02-02
申请号：US738114
申请日：1996-10-25
申请人： Tomokazu Matsue , Hitoshi Shiku , Isamu Uchida
发明人： Tomokazu Matsue , Hitoshi Shiku , Isamu Uchida
IPC分类号： C12Q1/00 , G01N27/00 , G01N27/30 , G01N27/327 , G01N27/416 , G01N33/543 , G01N37/00 , G01Q30/02 , G01Q30/10 , G01Q60/60 , G01Q80/00 , G01Q90/00 , C12Q1/70 , C12Q1/68 , G01N33/53 , G01N33/554
CPC分类号： C12Q1/001 , C12Q1/004 , G01N33/54373 , G01N33/5438
摘要： An analyte and a reactant 3 that reacts with the analyte either directly or indirectly are allowed to react with each other on the analytical areas A of a substrate 1 and signals originating from the reaction are detected. In at least a signal detection step, either a signal generation-related portion 4x or a detector is provided in a portion that is opposed to the substrate 1 and high and low areas are formed in either the substrate 1 or the opposed portion or both in such a manner that the distance from each analytical area A of the substrate to the opposed portion is shorter than the distance from each of the non-analytical areas B of the substrate to the opposed portion, whereby signals originating from the reaction in the analytical areas A will be detected at higher intensities than signals originating from the reaction in the non-analytical areas B. The analyte can be quantitatively or qualitatively analyzed on the analytical areas of the substrate in high precision with reduced effects from the non-analytical areas of the substrate. Simultaneous analysis of multiple samples or simultaneous analysis for multiple items can also be accomplished with a simple sensor configuration.
摘要翻译：可以直接或间接地与分析物反应的分析物和反应物3在基板1的分析区域A上彼此反应，并且检测来自反应的信号。在至少信号检测步骤中，在与基板1相对的部分中设置信号生成相关部分4x或检测器，并且在基板1或相对部分中形成高低区域，从衬底的每个分析区域A到相对部分的距离比从衬底的非分析区域B到相对部分的距离短的方式，由此源于分析区域中的反应的信号将在比非分析区域B中的反应信号更高的强度下检测到A。可以在基板的分析区域上以高精度定量或定性地分析分析物，并且从非分析区域的非分析区域基质。同时分析多个样本或同时分析多个项目也可以通过简单的传感器配置来实现。

3. 发明申请

US20120160679A1 ELECTRODE DEVICE FOR AN ELECTROCHEMICAL SENSOR CHIP 有权
标题翻译：电化学传感器芯片用电极装置
公开(公告)号：US20120160679A1
公开(公告)日：2012-06-28
申请号：US13311270
申请日：2011-12-05
申请人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Kumi Inoue , Tomokazu Matsue
发明人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Kumi Inoue , Tomokazu Matsue
IPC分类号： G01N27/30
CPC分类号： G01N27/307 , G01N27/403 , G01N33/487
摘要： An electrode device for an electrochemical sensor chip includes an insulation sheet having an insulating property and including a top surface and a bottom surface opposite to each other in a thickness direction, and electrode members having a conductivity and held by the insulation sheet with the electrode members piercing the insulation sheet in a thickness direction, one ends of the electrode members located on the top surface side of the insulation sheet being connected to an analyte, the other ends located on the bottom surface side of the insulation sheet being connected to an electrodes of a transducer, at least the one ends of the electrode members being made of a mixture of conductive particles and an insulating material.
摘要翻译：一种用于电化学传感器芯片的电极装置包括具有绝缘性并且包括在厚度方向上彼此相对的顶表面和底表面的绝缘片，以及具有导电性并由绝缘片保持的电极构件的电极构件在绝缘片的厚度方向上刺穿绝缘片，绝缘片的顶面侧的电极构件的一端与分析物连接，位于绝缘片的底面侧的另一端与电极换能器，电极构件的至少一端由导电颗粒和绝缘材料的混合物制成。

4. 发明申请

US20080248972A1 Method of Immobilizing Protein, Protein Chip, Method of Immobilizing Cell and Cell Chip 审中-公开
标题翻译：固定蛋白，蛋白芯片，固定化细胞和细胞芯片的方法
公开(公告)号：US20080248972A1
公开(公告)日：2008-10-09
申请号：US11579267
申请日：2005-03-10
申请人： Matsuhiko Nishizawa , Hirokazu Kaji , Tomokazu Matsue
发明人： Matsuhiko Nishizawa , Hirokazu Kaji , Tomokazu Matsue
IPC分类号： C40B50/18 , C07K14/00 , C07K16/00 , C12N11/08 , C07K14/76
CPC分类号： G01N33/543 , B01J2219/00659 , B01J2219/00725 , B01J2219/00743
摘要： It is intended to provide a novel method of immobilizing a protein and a protein chip, by which the protein can be immobilized at a high reproducibility while preventing the protein from inactivation without resort to a large-scaled apparatus and the protein can be immobilized even in a microchannel. Further, by using a cell adhesive protein as the protein to be immobilized, it is also possible to use a cell as a target and to provide a method of immobilizing a cell and a cell chip, by which a cell can be immobilized in an arbitrary region on a substrate.
摘要翻译：旨在提供一种固定蛋白质和蛋白质芯片的新方法，通过该方法可以以高再现性固定蛋白质，同时防止蛋白质失活而不需要大规模装置，并且即使在一个微通道。此外，通过使用细胞粘附蛋白作为待固定的蛋白质，还可以使用细胞作为靶，并提供固定细胞和细胞芯片的方法，通过其可以将细胞固定在任意的位置区域。

5. 发明授权

US08470144B2 Electrode device for an electrochemical sensor chip 有权
标题翻译：电化学传感器芯片用电极装置
公开(公告)号：US08470144B2
公开(公告)日：2013-06-25
申请号：US13311255
申请日：2011-12-05
申请人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Kosuke Ino , Tomokazu Matsue
发明人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Kosuke Ino , Tomokazu Matsue
IPC分类号： G01N27/416
CPC分类号： G01N27/3272
摘要： An electrode device for an electrochemical sensor chip includes an insulation sheet having an insulating property and including a top surface and a bottom surface opposite to each other in a thickness direction, and electrode members having a conductivity and held by the insulation sheet in portions piercing the insulation sheet in a thickness direction, one ends of the electrode members located on the top surface side of the insulation sheet being connected to the analyte, other ends located on the bottom surface side of the insulation sheet being connected to the electrodes of the transducer, recesses for trapping the analyte being formed in the top surface of the insulation sheet so as to correspond to the electrode members, the one ends of the electrode members being exposed at a bottom of the recesses.
摘要翻译：一种用于电化学传感器芯片的电极装置，包括具有绝缘性并且在厚度方向上彼此相对的顶表面和底表面的绝缘片，以及具有导电性的电极构件，并且被绝缘片保持部分刺穿在绝缘片的厚度方向上，位于绝缘片的上表面侧的电极构件的一端与分析物连接，位于绝缘片的底面侧的另一端与换能器的电极连接，用于将形成在绝缘片的顶表面中的分析物捕获以与电极构件对应的凹部，电极构件的一端露出在凹部的底部。

6. 发明授权

US07544438B2 Electron mediator, electron mediator immobilized electrode, and biofuel cell using the electrode 有权
标题翻译：电子介体，电子介体固定电极和使用电极的生物燃料电池
公开(公告)号：US07544438B2
公开(公告)日：2009-06-09
申请号：US10807489
申请日：2004-03-23
申请人： Matsuhiko Nishizawa , Tomokazu Matsue , Jun-ichi Kosuge , Noboru Fukasaku
发明人： Matsuhiko Nishizawa , Tomokazu Matsue , Jun-ichi Kosuge , Noboru Fukasaku
IPC分类号： H01M2/14 , H01M4/86 , C12Q1/00 , C12N11/08
CPC分类号： C07C66/02 , C07C235/78 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An object of the present invention is to provide a highly functional mediator capable of smoothly proceeding electron transfer between an electrode and a biocatalyst based on the premise that the mediator excels in safety and cost and to provide an electrode and a cell with sufficient amount of such a mediator molecule immobilized efficiently and strongly. The present invention provides a mediator mediating electron transfer between an enzyme and an electrode and containing a quinone molecule derivative, in particular, a mediator containing a VK3 derivative. In addition, the present invention provides an electrode and a biofuel cell to which those mediators are applied.
摘要翻译：本发明的目的是提供一种能够平稳地进行电极和生物催化剂之间的电子转移的高功能介体，其基于这样的前提，即介质优良的安全性和成本，并且提供具有足够量的这样的电极和电池一种有效强固地固定的介体分子。本发明提供介导酶和电极之间的电子转移并含有醌分子衍生物，特别是含有VK3衍生物的介体的介体。此外，本发明提供一种电极和生物燃料电池，其中应用这些介体。

7. 发明授权

US08691062B2 Electrode device for an electrochemical sensor chip 有权
标题翻译：电化学传感器芯片用电极装置
公开(公告)号：US08691062B2
公开(公告)日：2014-04-08
申请号：US13311270
申请日：2011-12-05
申请人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Kumi Inoue , Tomokazu Matsue
发明人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Kumi Inoue , Tomokazu Matsue
IPC分类号： G01N27/416 , G01N27/30 , G01N33/487
CPC分类号： G01N27/307 , G01N27/403 , G01N33/487
摘要： An electrode device for an electrochemical sensor chip includes an insulation sheet having an insulating property and including a top surface and a bottom surface opposite to each other in a thickness direction, and electrode members having a conductivity and held by the insulation sheet with the electrode members piercing the insulation sheet in a thickness direction, one ends of the electrode members located on the top surface side of the insulation sheet being connected to an analyte, the other ends located on the bottom surface side of the insulation sheet being connected to an electrodes of a transducer, at least the one ends of the electrode members being made of a mixture of conductive particles and an insulating material.
摘要翻译：一种用于电化学传感器芯片的电极装置包括具有绝缘性并且包括在厚度方向上彼此相对的顶表面和底表面的绝缘片，以及具有导电性并由绝缘片保持的电极构件的电极构件在绝缘片的厚度方向上刺穿绝缘片，绝缘片的顶面侧的电极构件的一端与分析物连接，位于绝缘片的底面侧的另一端与电极换能器，电极构件的至少一端由导电颗粒和绝缘材料的混合物制成。

8. 发明授权

US5283181A Conjugated enzyme electrode 失效
标题翻译：共轭酶电极
公开(公告)号：US5283181A
公开(公告)日：1994-02-01
申请号：US672238
申请日：1991-03-20
申请人： Isamu Uchida , Tomokazu Matsue , Hsien-Chang Chang , Akinori Ueno , Hiroshi Yamada
发明人： Isamu Uchida , Tomokazu Matsue , Hsien-Chang Chang , Akinori Ueno , Hiroshi Yamada
IPC分类号： G01N27/327 , C12Q1/00 , C12Q1/32 , C12N1/00
CPC分类号： C12Q1/005 , C12Q1/003 , Y10S435/817 , Y10S435/832
摘要： A conjugated enzyme electrode comprising diaphorase and an amino-acid dehydrogenase immobilized on a conductive support by a polyfunctional aldehyde is disclosed, by which amino acids can be rapidly determined with high sensitivity.
摘要翻译：公开了通过多官能醛固定在导电性支持体上的包含心肌黄酶和氨基酸脱氢酶的共轭酶电极，可以高灵敏度地迅速测定氨基酸。

9. 发明授权

US08691061B2 Electrode device for an electrochemical sensor chip 有权
标题翻译：电化学传感器芯片用电极装置
公开(公告)号：US08691061B2
公开(公告)日：2014-04-08
申请号：US13311237
申请日：2011-12-05
申请人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Tomokazu Matsue
发明人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Tomokazu Matsue
IPC分类号： G01N27/416 , G01N27/30 , G01N33/487
CPC分类号： G01N27/307 , G01N27/403 , G01N33/487
摘要： An electrode device for an electrochemical sensor chip includes an insulation sheet having an insulating property and including a top surface and a bottom surface opposite to each other in a thickness direction, and electrode members having a conductivity and held by the insulation sheet with the electrode members piercing the insulation sheet in a thickness direction, one ends of the electrode members located on the top surface side of the insulation sheet being connected to an analyte, the other ends located on the bottom surface side of the insulation sheet being connected to an electrodes of a transducer.
摘要翻译：一种用于电化学传感器芯片的电极装置包括具有绝缘性并且包括在厚度方向上彼此相对的顶表面和底表面的绝缘片，以及具有导电性并由绝缘片保持的电极构件的电极构件在绝缘片的厚度方向上刺穿绝缘片，绝缘片的顶面侧的电极构件的一端与分析物连接，位于绝缘片的底面侧的另一端与电极传感器。

10. 发明申请

US20120160678A1 ELECTRODE DEVICE FOR AN ELECTROCHEMICAL SENSOR CHIP 有权
标题翻译：电化学传感器芯片用电极装置
公开(公告)号：US20120160678A1
公开(公告)日：2012-06-28
申请号：US13311255
申请日：2011-12-05
申请人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Kosuke Ino , Tomokazu Matsue
发明人： Atsushi Suda , Tatsuo Kimura , Ryota Kunikata , Kosuke Ino , Tomokazu Matsue
IPC分类号： G01N27/30
CPC分类号： G01N27/3272
摘要： An electrode device for an electrochemical sensor chip includes an insulation sheet having an insulating property and including a top surface and a bottom surface opposite to each other in a thickness direction, and electrode members having a conductivity and held by the insulation sheet in portions piercing the insulation sheet in a thickness direction, one ends of the electrode members located on the top surface side of the insulation sheet being connected to the analyte, other ends located on the bottom surface side of the insulation sheet being connected to the electrodes of the transducer, recesses for trapping the analyte being formed in the top surface of the insulation sheet so as to correspond to the electrode members, the one ends of the electrode members being exposed at a bottom of the recesses.
摘要翻译：一种用于电化学传感器芯片的电极装置，包括具有绝缘性并且在厚度方向上彼此相对的顶表面和底表面的绝缘片，以及具有导电性的电极构件，并且被绝缘片保持部分刺穿在绝缘片的厚度方向上，位于绝缘片的上表面侧的电极构件的一端与分析物连接，位于绝缘片的底面侧的另一端与换能器的电极连接，用于将形成在绝缘片的顶表面中的分析物捕获以与电极构件对应的凹部，电极构件的一端露出在凹部的底部。

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