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    • 5. 发明申请
    • UDP-galactose: beta-N acetyl-glucosamine beta-1, 4-galactosyltransferase, beta4 Gal-T2
    • UDP-半乳糖:β-N乙酰葡糖胺β-1,4-半乳糖基转移酶,β4Gal-T2
    • US20050181437A1
    • 2005-08-18
    • US11105796
    • 2005-04-13
    • Henrik ClausenEric Bennett
    • Henrik ClausenEric Bennett
    • C12N9/10C12Q1/68C07H21/04
    • C12N9/1051
    • A novel gene defining a novel enzyme in the UDP-D-galactose: b-N-acetyl-glucosamine β-1,4-galactosyltransferase family, termed β4Gal-T2, with unique enzymatic properties is disclosed. The enzymatic activity of β4Gal-T2 is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs encoding β4Gal-T2 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting β4Gal-T2 activity, as well as cloning and expression vectors including such DNA, cells transfected with the vectors, and recombinant methods for providing β4Gal-T2. The enzyme β4Gal-T2 and β4Gal-T2-active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of β4Gal-T2. Further, the invention discloses methods of obtaining β-1,4-galactosyl glycosylated saccharides, glycopeptides or glycoproteins by use of an enzymically active β4Gal-T2 protein or fusion protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active β4Gal-T2 protein as an expression system for recombinant production of such glycopeptides or glycoproteins. Also a method for the identification of DNA sequence variations in the β4Gal-T2 gene by isolating DNA from a patient, amplifying β4Gal-T2-coding exons by PCR, and detecting the presence of DNA sequence variation, are disclosed.
    • 公开了一种在UDP-D-半乳糖:b-N-乙酰葡糖胺β-1,4-半乳糖基转移酶家族中定义新的酶的新基因,称为β4Gal-T2,具有独特的酶学性质。 β4Gal-T2的酶活性显示与以前鉴定的该基因家族的酶不同。 本发明公开了分离的DNA分子和编码β4Gal-T2的DNA构建体及其衍生物,通过氨基酸缺失,取代或插入显示β4Gal-T2活性,以及​​克隆和表达载体,包括这种DNA,用载体转染的细胞,以及 提供β4Gal-T2的重组方法。 公开了酶β4Gal-T2和β4Gal-T2-活性衍生物,特别是包含β4Gal-T2的催化活性结构域的可溶性衍生物。 此外,本发明公开了通过使用酶活性β4Gal-T2蛋白或其融合蛋白或通过使用包含编码酶活性的DNA的载体稳定转染的细胞获得β-1,4-半乳糖基糖基化糖,糖肽或糖蛋白的方法 β4Gal-T2蛋白作为重组生产这种糖肽或糖蛋白的表达系统。 还公开了通过从患者中分离DNA,通过PCR扩增β4Gal-T2编码外显子并检测DNA序列变异的存在来鉴定β4Gal-T2基因中的DNA序列变异的方法。
    • 8. 发明申请
    • UDP-galactose: beta-D-galactose-R4-alpha-D-galactosyltransferase, alpha4Gal-T1
    • UDP-半乳糖:β-D-半乳糖-R4-α-D-半乳糖基转移酶,α4Gal-T1
    • US20060099688A1
    • 2006-05-11
    • US11317196
    • 2005-12-22
    • Henrik ClausenRudi SteffensenEric Bennett
    • Henrik ClausenRudi SteffensenEric Bennett
    • C12P21/06C12P19/04C07H21/04C12N9/10
    • C12N9/1051
    • A novel gene defining a novel enzyme UDP-galactose: β-D-galactose-R 4-α-D-galactosyltransferase, termed α4Gal-T1, with unique enzymatic properties is disclosed. The invention provides isolated DNA molecules and DNA constructs encoding α4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting α4Gal-T1 activity, as well as cloning and expression vectors including such DNA, host cells comprising DNA encoding α4Gal-T1, and recombinant methods for providing α4Gal-T1. The enzyme α4Gal-T1 and α4Gal-active derivatives thereof are disclosed. Further, the invention discloses methods of obtaining α1, 4galactosyl glycosylated glycosphingolipids by use of an enzymatically active α4Gal-T1 protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active α4Gal-T1 protein as an expression system for recombinant production of such glycosphingolipids. Also a method for the identification of DNA sequence variations in the α4Gal-T1-coding exon by PCR, and detecting the presence of DNA sequence variation, are disclosed.
    • 公开了一种新颖的基因,其定义了具有独特的酶学性质的新型酶UDP-半乳糖:β-D-半乳糖-R 4-α-D-半乳糖基转移酶,称为α4Gal-T1。 本发明提供分离的DNA分子和编码α4Gal-T1及其衍生物的DNA构建体,其通过氨基酸缺失,取代或插入表现出α4Gal-T1活性,以及​​克隆和表达载体,包括此类DNA,宿主细胞包含编码α4Gal- T1和用于提供α4Gal-T1的重组方法。 公开了其α4Gal-T1和α4Gal活性衍生物。 此外,本发明公开了通过使用其酶促活化的α4Gal-T1蛋白或通过使用包含编码酶活性α4Gal-T1蛋白的DNA稳定转染的细胞作为重组生产的表达系统来获得α1,4半乳糖基糖基化糖苷脂的方法 的这种糖鞘醇。 还公开了通过PCR鉴定α4Gal-T1编码外显子中的DNA序列变异并检测DNA序列变异的存在的方法。