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1. 发明授权

US07067324B2 Photon reducing agents for fluorescence assays 有权
公开(公告)号：US07067324B2
公开(公告)日：2006-06-27
申请号：US09759629
申请日：2001-01-12
申请人： Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Tim Rink
发明人： Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Tim Rink
IPC分类号： C12Q1/70 , G01N21/76
CPC分类号： G01N33/582 , C12Q1/682 , G01N33/5005 , G01N33/542 , Y10S436/80 , Y10S436/809 , Y10T436/13 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.

2. 发明授权

US06214563B1 Photon reducing agents for reducing undesired light emission in assays 失效
标题翻译：用于在测定中减少不期望的光发射的光子还原剂
公开(公告)号：US06214563B1
公开(公告)日：2001-04-10
申请号：US09120516
申请日：1998-07-21
申请人： Paul Negulescu , Gregor Zlokarnik , Tom Knapp , Roger Y. Tsien , Tim Rink
发明人： Paul Negulescu , Gregor Zlokarnik , Tom Knapp , Roger Y. Tsien , Tim Rink
IPC分类号： C12Q170
CPC分类号： G01N33/542 , C12Q1/34 , C12Q1/682 , G01N33/5005 , G01N2415/00 , Y10S436/80 , Y10S436/809 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions and kits for practicing these methods.
摘要翻译：本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供使用至少一种光子还原剂测定样品中结合和游离分析物的方法。本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的方法。本发明还提供了用于实施这些方法的组合物和试剂盒。

3. 发明授权

US06200762B1 Photon reducing agents and compositions for fluorescence assays 失效
标题翻译：光子还原剂和用于荧光测定的组合物
公开(公告)号：US06200762B1
公开(公告)日：2001-03-13
申请号：US09118497
申请日：1998-07-17
申请人： Gregor Zlokarnik , Paul Negulescu , Tom Knapp , Roger Y. Tsien , Tim Rink
发明人： Gregor Zlokarnik , Paul Negulescu , Tom Knapp , Roger Y. Tsien , Tim Rink
IPC分类号： C12Q170
CPC分类号： G01N33/542 , C12Q1/682 , G01N33/5005 , Y10S436/80 , Y10S436/809 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions and kits for practicing these methods.
摘要翻译：本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供使用至少一种光子还原剂测定样品中结合和游离分析物的方法。本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的方法。本发明还提供了用于实施这些方法的组合物和试剂盒。

4. 发明授权

US06221612B1 Photon reducing agents for use in fluorescence assays 失效
标题翻译：用于荧光测定的光子还原剂
公开(公告)号：US06221612B1
公开(公告)日：2001-04-24
申请号：US09122477
申请日：1998-07-23
申请人： Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Tim Rink
发明人： Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Tim Rink
IPC分类号： C12Q170
CPC分类号： G01N33/582 , C12Q1/682 , G01N33/5005 , G01N33/542 , Y10S436/80 , Y10S436/809 , Y10T436/13 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
摘要翻译：本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的系统和方法。本发明还提供了用于实施这些方法的组合物，药物组合物和试剂盒。

5. 发明申请

US20090162926A1 PHOTON REDUCING AGENTS FOR FLUORESCENCE ASSAYS 有权
标题翻译：用于荧光测定的光子还原剂
公开(公告)号：US20090162926A1
公开(公告)日：2009-06-25
申请号：US12398744
申请日：2009-03-05
申请人： Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Timothy James Rink
发明人： Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Timothy James Rink
IPC分类号： C12M1/34
CPC分类号： G01N33/582 , C12Q1/682 , G01N33/5005 , G01N33/542 , Y10S436/80 , Y10S436/809 , Y10T436/13 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g. a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
摘要翻译：本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。本发明还提供了一种用至少一种光子产生剂和至少一种碰撞猝灭剂来减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的系统和方法。本发明还提供了用于实施这些方法的组合物，药物组合物和试剂盒。

6. 发明申请

US20110076711A1 PHOTON REDUCING AGENTS FOR FLUORESCENCE ASSAYS 有权
标题翻译：用于荧光测定的光子还原剂
公开(公告)号：US20110076711A1
公开(公告)日：2011-03-31
申请号：US12861791
申请日：2010-08-23
申请人： Tom KNAPP , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Timothy James Rink
发明人： Tom KNAPP , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Timothy James Rink
IPC分类号： C12Q1/02
CPC分类号： G01N33/582 , C12Q1/682 , G01N33/5005 , G01N33/542 , Y10S436/80 , Y10S436/809 , Y10T436/13 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
摘要翻译：本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的系统和方法。本发明还提供了用于实施这些方法的组合物，药物组合物和试剂盒。

7. 发明申请

US20130004960A1 PHOTON REDUCING AGENTS FOR FLUORESCENCE ASSAYS 有权
标题翻译：用于荧光测定的光子还原剂
公开(公告)号：US20130004960A1
公开(公告)日：2013-01-03
申请号：US13437846
申请日：2012-04-02
申请人： Tom KNAPP , Gregor Zlokarnik , Paul Negulesou , Roger Y. Tsien , Timothy James Rink
发明人： Tom KNAPP , Gregor Zlokarnik , Paul Negulesou , Roger Y. Tsien , Timothy James Rink
IPC分类号： G01N21/64 , G01N21/75
CPC分类号： G01N33/582 , C12Q1/682 , G01N33/5005 , G01N33/542 , Y10S436/80 , Y10S436/809 , Y10T436/13 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
摘要翻译：本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的系统和方法。本发明还提供了用于实施这些方法的组合物，药物组合物和试剂盒。

8. 发明申请

US20050136469A1 Photon reducing agents for fluorescence assays 审中-公开
标题翻译：用于荧光测定的光子还原剂
公开(公告)号：US20050136469A1
公开(公告)日：2005-06-23
申请号：US11047074
申请日：2005-01-31
申请人： Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Tsien , Timothy Rink
发明人： Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Tsien , Timothy Rink
IPC分类号： C12Q1/68 , G01N33/50 , G01N33/542 , G01N33/58 , G01N33/53 , G01N33/567
CPC分类号： G01N33/582 , C12Q1/682 , G01N33/5005 , G01N33/542 , Y10S436/80 , Y10S436/809 , Y10T436/13 , C12Q2563/107 , C12Q2527/125
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
摘要翻译：本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的系统和方法。本发明还提供了用于实施这些方法的组合物，药物组合物和试剂盒。

9. 发明授权

US08071761B2 Substrates for beta-lactamase and uses thereof 有权
标题翻译： β-内酰胺酶底物及其用途
公开(公告)号：US08071761B2
公开(公告)日：2011-12-06
申请号：US11606642
申请日：2006-11-29
申请人： Roger Y. Tsien , Gregor Zlokarnik
发明人： Roger Y. Tsien , Gregor Zlokarnik
IPC分类号： C07D501/14
CPC分类号： C07D501/36 , C07D501/00 , C07D505/00 , C07D519/00 , C12N9/86 , C12N15/85 , C12N2800/90 , C12N2830/002 , C12P17/184 , C12P17/188 , C12P35/00 , C12Q1/18 , C12Q1/34 , C12Q1/6897 , C12Q2334/00 , G01N2333/986 , G01N2415/00 , Y10S435/805
摘要： Substrates for β-lactamase of the general formula I in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit); R′ is selected from the group consisting of H, lower (i.e., alkyl of 1 to about 5 carbon atoms) and (CH2)nOH, in which n is 0 or an integer from 1 to 5; R″ is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, —CHR2OCO(CH2)nCH3, —CHR2OCOC (CH3)3, acylthiomethyl, acyloxy-alpha-benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, acyloxyalkyl, dialkylaminocarbonyloxymethyl and aliphatic, in which R2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO2 and CH2; and Z′ and Z″ are linkers for the fluorescent donor and quencher moieties. Methods of assaying β-lactamase activity and monitoring expression in systems using β-lactamase as a reporter gene also are disclosed.
摘要翻译： X和Y中的一个是荧光供体部分，另一个是猝灭剂（其可以或不再发射）的通式I的β-内酰胺酶底物; R'选自H，低级（即1至约5个碳原子的烷基）和（CH 2）nOH，其中n为0或1至5的整数; -CH 2 COO（CH 2）n CH 3，-CHR 2 OCOC（CH 3）3，酰基硫代甲基，酰氧基-α-苄基，δ-丁基内酰胺，甲氧基羰氧基甲基，苯基，甲基亚磺酰基甲基， β-吗啉基乙基，二烷基氨基乙基，酰氧基烷基，二烷基氨基羰氧基甲基和脂族基，其中R2选自H和低级烷基; A选自S，O，SO，SO 2和CH 2; 和Z'和Z“是荧光供体和猝灭剂部分的接头。公开了使用β-内酰胺酶作为报道基因的系统中测定β-内酰胺酶活性和监测表达的方法。

10. 发明授权

US6031094A Beta-lactam substrates and uses thereof 失效
标题翻译： β-内酰胺底物及其用途
公开(公告)号：US6031094A
公开(公告)日：2000-02-29
申请号：US128679
申请日：1998-07-23
申请人： Roger Y. Tsien , Gregor Zlokarnik
发明人： Roger Y. Tsien , Gregor Zlokarnik
IPC分类号： C07D205/08 , C07D401/04 , C07D403/04 , C07D405/04 , C07D405/10 , C07D205/085
CPC分类号： C07D401/04 , C07D205/08 , C07D403/04 , C07D405/04 , C07D405/10
摘要： ##STR1## Substrates for beta-lactamase of general formula 1, in which R
1 is a moiety, in which a primary amine attached to an aryl carbon of a fluorophore precursor was converted into the nitrogen atom of the beta-lactam ring and the fluorophore precursor is not anthranilic acid when R
2 is H; R
2 is selected from the group consisting of H and --NR
3 R
4 , in which R
3 and R
4 are selected from the group consisting of H, aliphatic, alkyl, and acyl; and the beta-lactam ring may be cleaved by a beta-lactamase enzyme. Methods of assaying beta-lactamase activity and monitoring expression in systems using beta-lactamase as a reporter gene are also disclosed.
摘要翻译：用于通式1的β-内酰胺酶的底物，其中R1是部分，其中连接到荧光团前体的芳基碳上的伯胺转化成β-内酰胺环的氮原子和荧光团前体不是邻氨基当R2为H时为酸; R 2选自H和-NR 3 R 4，其中R 3和R 4选自H，脂族，烷基和酰基; 并且β-内酰胺环可以被β-内酰胺酶切割。还公开了使用β-内酰胺酶作为报告基因的系统中测定β-内酰胺酶活性和监测表达的方法。

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