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1. 发明授权

US07148021B2 Human zona pellucida proteins and methods of their use in diagnosing male infertility 失效
标题翻译：人透明质酸蛋白及其用于诊断男性不育症的方法
公开(公告)号：US07148021B2
公开(公告)日：2006-12-12
申请号：US10209664
申请日：2002-08-01
申请人： Ting-Fung Chi , Zhiyong Lin , Ke-Wen Dong , Ming-I Hsu , Jr-Gang Cheng , Heming Zheng , Sergio C. Oehninger , William E. Gibbons
发明人： Ting-Fung Chi , Zhiyong Lin , Ke-Wen Dong , Ming-I Hsu , Jr-Gang Cheng , Heming Zheng , Sergio C. Oehninger , William E. Gibbons
IPC分类号： G01N33/53
CPC分类号： C07K14/705 , G01N33/56966 , G01N33/689 , G01N2800/367
摘要： Reagents, methods, and kits are described that are useful for fertility testing and that in many cases provides much faster, convenient and rapid determination of male infertility. The reagents described include properly glycosylated human ZP3, ZP2, ZP1, glycosylated peptides thereof, fusion proteins such as green fluorescent protein—ZP3, non-covalent complexes of ZP2 with ZP3, fusion protein of ZP2–ZP3, solid phase materials such as agarose beads coated with binding agents such as ZP3, and other artificial zona. Methods are provided that, in many cases convert a complex biological event into a well defined biochemical binding event based on one or more of the reagents. Such methods are much easier to set up and monitor, allowing more convenient and inexpensive diagnostic testing for male fertility. The acrosome reaction is detected in other embodiments by virtue of quantitating one or more released substances. Kits are further provided that contain one or more reagents useful for testing at a diagnostic laboratory or other facility.
摘要翻译：描述了可用于生殖力测试的试剂，方法和试剂盒，并且在许多情况下提供更快，更方便和快速测定男性不育症。所述试剂包括合适的糖基化人ZP3，ZP2，ZP1，其糖基化肽，融合蛋白如绿色荧光蛋白-ZP3，ZP2与ZP3的非共价复合物，ZP2-ZP3的融合蛋白，固相物质如琼脂糖珠涂覆有粘合剂如ZP3和其他人造纤维。提供的方法是，在许多情况下，基于一种或多种试剂将复杂的生物事件转化为明确的生物化学结合事件。这种方法更易于建立和监测，为男性生育提供更方便和便宜的诊断测试。在其它实施方案中，通过定量一种或多种释放物质来检测顶体反应。还提供了包含一种或多种可用于在诊断实验室或其他设施进行测试的试剂的试剂盒。

2. 发明授权

US07037663B2 Human zona pellucida protein 3 and uses thereof 失效
标题翻译：人透明质酸蛋白3及其用途
公开(公告)号：US07037663B2
公开(公告)日：2006-05-02
申请号：US09829073
申请日：2001-04-09
申请人： Ke-Wen Dong , Sergio C. Oehninger , William E. Gibbons
发明人： Ke-Wen Dong , Sergio C. Oehninger , William E. Gibbons
IPC分类号： G01N33/53
CPC分类号： C07K14/705
摘要： The present invention provides a method to determine sperm activity comprising the steps of: (a) contacting an appropriate concentration of human zona pellucida protein 3 with an appropriate amount of sperm under conditions permitting the formation of a complex between the human zona pellucida protein 3 and the sperm; and (b) determining the complex formed. The invention further provides a method to determine sperm activity comprising the steps of (a) contacting an appropriate concentration of human zona pellucida protein 3 with an appropriate amount of sperm under conditions permitting an acrosome reaction to occur; and (b) determining the extent of the acrosome reaction. Finally, this invention provides a diagnosis kit for sperm activity comprising three (3) compartments with (a) an appropriate amount of human zona pellucida protein 3; (b) the reagents used for establishing the conditions for allowing the binding of sperm; and (c) the reagents used for establishing the conditions for allowing an acrosome reaction.
摘要翻译：本发明提供了一种测定精子活性的方法，其包括以下步骤：（a）在适当浓度的人透明质酸蛋白3与适当量的精子接触的条件下，允许在人透明质酸蛋白3和精子和（b）确定形成的复合物。本发明还提供了一种测定精子活性的方法，其包括以下步骤：（a）在允许顶体反应发生的条件下，使适当浓度的人透明质酸蛋白3与适量的精子接触; 和（b）确定顶体反应的程度。最后，本发明提供了一种用于精子活动的诊断试剂盒，其包括三个（3）隔室，其中（a）适量的透明透明质酸蛋白3; （b）用于确定允许精子结合的条件的试剂; 和（c）用于确定允许顶体反应的条件的试剂。

3. 发明授权

US07019114B2 Recombinant, biologically active human zona pellucida protein 3 (HZP3) to test male fertility 失效
标题翻译：重组，生物活性人透明质酸蛋白3（HZP3）测试男性生殖力
公开(公告)号：US07019114B2
公开(公告)日：2006-03-28
申请号：US09252828
申请日：1999-02-19
申请人： Ke-Wen Dong , Sergio Oehninger , William E. Gibbons
发明人： Ke-Wen Dong , Sergio Oehninger , William E. Gibbons
IPC分类号： C07K14/00 , C07K1/00
CPC分类号： C07K14/705 , Y10S530/827
摘要： The invention relates to methods for producing recombinant human zona pellucida protein (“rhZP3”) and glycosylated peptide having biological activity of binding to human spermatozoa. A human ovarian cell line is used to produce rhZP3 having a glycosylation pattern required for full biological activity. Methods of determining useful peptides with binding activity for human sperm and their syntheses, as well as using such peptides and proteins in therapeutics and diagnostics are discussed.
摘要翻译：本发明涉及具有与人类精子结合的生物活性的重组人透明带蛋白（“rhZP3”）和糖基化肽的方法。使用人卵巢细胞系产生具有完全生物活性所需的糖基化模式的rhZP3。讨论了确定对人精子及其合成具有结合活性的有用肽以及在治疗和诊断中使用这些肽和蛋白质的方法。

4. 发明授权

US4892830A Environmentally controlled in vitro incubator 失效
标题翻译：环境控制的体外培养箱
公开(公告)号：US4892830A
公开(公告)日：1990-01-09
申请号：US36061
申请日：1987-04-02
申请人： William Findley , William E. Gibbons
发明人： William Findley , William E. Gibbons
IPC分类号： B01L1/00 , B25J21/02 , C12M1/00
CPC分类号： C12M21/06 , B01L1/00 , B25J21/02 , C12M23/22 , C12M41/06 , C12M41/14 , C12M41/34
摘要： An environmentally controlled incubator is described in which the enclosure of the incubator chamber strongly attenuates the transmission of light having wavelengths below about 500 nm for protecting biological materials within the chamber from toxic effects of short wavelength light. The incubator also includes sensors for determining the oxygen and carbon dioxide concentration within the chamber and means for adding carbon dioxide, nitrogen or oxygen to the ambient gas within the incubator ion order to maintain the desired levels of carbon dioxide and oxygen. A thermostatically controlled heater and a humidification chamber warm and humidify the gas as it is circulated through the humidification chamber under control of a fan. An airlock is provided to allow biological materials to be placed within or removed from the chamber.
摘要翻译：描述了一种环境受控的培养箱，其中培养箱的外壳强烈衰减波长低于约500nm的光的透射，以保护室内的生物材料免受短波长光的毒性作用。培养箱还包括用于确定室内的氧气和二氧化碳浓度的传感器，以及用于在孵育器离子顺序内向环境气体中加入二氧化碳，氮或氧的装置，以保持所需水平的二氧化碳和氧气。恒温控制加热器和加湿室在风扇控制下通过加湿室循环加热和加湿气体。提供气闸以允许将生物材料放置在室内或从腔室中移除。

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