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1. 发明申请

US20100086979A1 PRODUCTION OF OMEGA-3 FATTY ACIDS IN MICROFLORA OF THRAUSTOCHYTRIALES USING MODIFIED MEDIA 审中-公开
标题翻译：使用修改后的媒体生产谷类微生物中的OMEGA-3脂肪酸
公开(公告)号：US20100086979A1
公开(公告)日：2010-04-08
申请号：US12446776
申请日：2007-10-09
申请人： Thomas Kiy , Markus Luy , Oliver Zeumer
发明人： Thomas Kiy , Markus Luy , Oliver Zeumer
IPC分类号： C12P7/64 , C12P1/00
CPC分类号： C12P7/6472 , C12P7/6427
摘要： The present invention relates to improved methods for the production of omega-3 fatty acids by microorganisms belonging to the microflora of Thraustochytriales using a modified composition of the culture medium. In particular, the invention is directed to a process for the production of omega-3 fatty acids by culturing microflora, such as strains of Ulkenia, Thraustochytrium and/or Schizochytrium, in fermentors which includes the step of culturing the Thraustochytriales microflora in an environment of reduced sodium ions and increased potassium ions.
摘要翻译：本发明涉及使用改良的培养基组合物来产生属于破囊壶菌菌群的微生物的ω-3脂肪酸的改进方法。特别地，本发明涉及通过在发酵罐中培养微生物菌群如乌尔金氏菌属，破囊壶菌属和/或裂殖壶菌属的菌株来生产ω-3脂肪酸的方法，该方法包括在环境中培养破囊壶菌菌群的步骤减少钠离子和增加钾离子。

2. 发明申请

US20090093033A1 PUFA-PKS Genes From Ulkenia 有权
标题翻译：来自Ulkenia的PUFA-PKS基因
公开(公告)号：US20090093033A1
公开(公告)日：2009-04-09
申请号：US11547921
申请日：2005-04-08
申请人： Markus Luy , Matthias Rusing , Thomas Kiy
发明人： Markus Luy , Matthias Rusing , Thomas Kiy
IPC分类号： C12P7/64 , C12N9/00 , C12N15/11 , C11B1/00 , C12N15/00 , C12N1/21
CPC分类号： C12P7/6472 , C12N15/52
摘要： The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.
摘要翻译：本发明涉及编码针对聚酮化合物合酶（PKS）特异性序列的基因。由此合成的PKS的特征在于其产生PUFA（多不饱和脂肪酸）的酶能力。除了使用所述核苷酸序列产生重组和/或转基因生物外，本发明还涉及鉴定相应的DNA序列。

3. 发明授权

US07939305B2 PUFA-PKS genes from Ulkenia 有权
标题翻译：来自Ulkenia的PUFA-PKS基因
公开(公告)号：US07939305B2
公开(公告)日：2011-05-10
申请号：US11547921
申请日：2005-04-08
申请人： Markus Luy , Matthias Rüsing , Thomas Kiy
发明人： Markus Luy , Matthias Rüsing , Thomas Kiy
IPC分类号： C12N9/00
CPC分类号： C12P7/6472 , C12N15/52
摘要： The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.
摘要翻译：本发明涉及编码针对聚酮化合物合酶（PKS）特异性序列的基因。由此合成的PKS的特征在于其产生PUFA（多不饱和脂肪酸）的酶能力。除了使用所述核苷酸序列产生重组和/或转基因生物外，本发明还涉及鉴定相应的DNA序列。

4. 发明申请

US20070054384A1 Method for the cultivation of microorganisms of the genus thraustochytriales by using an optimized low salt medium 有权
标题翻译：通过使用优化的低盐培养基培养破囊壶菌属微生物的方法
公开(公告)号：US20070054384A1
公开(公告)日：2007-03-08
申请号：US10578968
申请日：2004-11-10
申请人： Matthias Rusing , Markus Luy
发明人： Matthias Rusing , Markus Luy
IPC分类号： C12P7/64 , C12N1/21 , A23K1/00
CPC分类号： C12P7/6427 , A23K10/37 , A23L33/12 , A23V2002/00 , C12N1/12 , C12P7/6472
摘要： The invention relates to an optimized method for cultivating microorganisms of the genus Thraustochytriales, according to which the microorganisms are cultivated in a low salt medium without adding sodium salts and chloride salts, the total salt content being less than 3.5 g/L (corresponding to less than 10 percent of sea water salt content), whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention especially relates to novel optimized media having a substantially reduced total salt content, above all a particularly reduced NaCl content. The production of PUFAs can be substantially improved and significantly simplified by using a novel combination of different salts as a media composition in which the overall weight ratios of ions do not exceed 1.75 g/L. Furthermore, said medium preferably contains no added sodium salt and chloride salt at all, which helps prevent environmental damages caused by wastewaters containing salt.
摘要翻译：本发明涉及一种用于培养破囊壶菌属微生物的优化方法，根据该方法，微生物在低盐培养基中培养而不添加钠盐和氯化物盐，总盐含量小于3.5g / L（相当于少于超过10％的海水盐含量），由此从微生物和/或介质中分离PUFA。本发明特别涉及具有显着降低的总盐含量，特别是特别降低的NaCl含量的新型优化的介质。通过使用不同盐的新型组合作为其中离子的总重量比不超过1.75g / L的介质组合物，可以显着改善和显着地简化PUFA的生产。此外，所述介质优选根本不含有添加的钠盐和氯化物盐，这有助于防止由含有盐的废水引起的环境损害。

5. 发明授权

US08889382B2 Process for cultivating microorganisms of the genus Thraustochytriales 有权
标题翻译：培养破囊壶菌属微生物的方法
公开(公告)号：US08889382B2
公开(公告)日：2014-11-18
申请号：US10578965
申请日：2004-11-10
申请人： Markus Luy , Matthias Rüsing
发明人： Markus Luy , Matthias Rüsing
IPC分类号： C12P7/64 , C12N1/00
CPC分类号： C12P7/6427 , C12N1/14 , C12P7/6472
摘要： The invention relates to an optimized method for the production of PUFAs by cultivating microorganisms belonging to the group of Stramenopiles in a fermentation medium that is pH-stabilized using calcium carbonate and comprises 3-15 g/L CaCO3, whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention particularly relates to novel optimized media having a different CaCO3 content. By using adequate quantities of CaCO3, the process can be significantly simplified during fermentation while greater quantities of DHA can be obtained at an increased oil content in the biomass. They allow microorganisms belonging to the Stramenopiles to be fermented without controlling the pH, thereby substantially improving and significantly simplifying PUFA production.
摘要翻译：本发明涉及通过在使用碳酸钙进行pH稳定化的发酵培养基中培养属于Stramenopiles组的微生物并且包含3-15g / L CaCO 3来生产PUFA的优化方法，由此将PUFA从微生物和/或培养基。本发明特别涉及具有不同CaCO3含量的新型优化介质。通过使用足够量的CaCO 3，可以在发酵期间显着简化该过程，同时在生物质中增加的油含量可获得更多量的DHA。它们允许属于Stramenopiles的微生物发酵而不控制pH值，从而显着改善和显着简化PUFA生产。

6. 发明授权

US08409798B2 Screening method for identifying PUFA PKS in samples 失效
标题翻译：用于鉴定样品中PUFA PKS的筛选方法
公开(公告)号：US08409798B2
公开(公告)日：2013-04-02
申请号：US11547948
申请日：2005-04-08
申请人： Markus Luy , Matthias Rüsing
发明人： Markus Luy , Matthias Rüsing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The invention relates to a method for the rapid and simple identification of PUFA PKS in microorganisms. Said method is characterized by the fact that DNA sections representing PKS that produce specifically for PUFA (polyunsaturated fatty acids) are reproduced in vitro. The PUFA PKS-specific amino acid sequence LGIDSIKRVEIL makes it possible to derive oligonucleotides which are used for the efficient PCR screening for PUFA PKS genes or PUFA-producing microorganisms. The inventive method is particularly suitable for the high throughput screening of microorganisms for PUFA PKS genes.
摘要翻译：本发明涉及用于快速和简单地鉴定微生物中PUFA PKS的方法。所述方法的特征在于代表PKS产生的专门用于PUFA（多不饱和脂肪酸）的DNA片段在体外再现。 PUFA PKS特异性氨基酸序列LGIDSIKRVEIL可以获得用于有效PCR筛选PUFA PKS基因或产生PUFA的微生物的寡核苷酸。本发明的方法特别适合用于PUFA PKS基因的微生物的高通量筛选。

7. 发明申请

US20110129884A1 METHOD FOR THE CULTIVATION OF MICROORGANISMS OF THE ORDER THRAUSTOCHYTRIALES 审中-公开
标题翻译：用于培养订购枸杞子微生物的方法
公开(公告)号：US20110129884A1
公开(公告)日：2011-06-02
申请号：US12742346
申请日：2008-11-21
申请人： Markus Luy
发明人： Markus Luy
IPC分类号： C12P7/64 , C12N1/00 , C07C59/255
CPC分类号： C12N1/14 , C12P7/6427
摘要： The present invention relates generally to the field of microorganism cell culture. It devises a cell culture medium for and a method of culturing microorganisms of the order Thraustochytriales, especially for the production of omega-3-fatty acids. Furthermore it devises the use of ammonium tartrate as nitrogen source for the cultivation of micro-organisms of the order Thraustochytriales.
摘要翻译：本发明一般涉及微生物细胞培养领域。它设计了一种培养细菌培养基和一种培养Thraustochytriales级微生物的方法，特别是用于生产ω-3-脂肪酸。此外，它设计了酒石酸铵作为氮源的用途，用于培养Thraustochytriales的微生物。

8. 发明申请

US20070259355A1 Screening Method for Identifying Pufa Pks in Samples 失效
标题翻译：在样品中识别Pufa Pks的筛选方法
公开(公告)号：US20070259355A1
公开(公告)日：2007-11-08
申请号：US11547948
申请日：2005-04-08
申请人： Markus Luy , Matthias Rusing
发明人： Markus Luy , Matthias Rusing
IPC分类号： C07H21/04 , C07K14/00 , C12N1/20 , C12Q1/68
CPC分类号： C12Q1/689
摘要： The invention relates to a method for the rapid and simple identification of PUFA PKS in microorganisms. Said method is characterized by the fact that DNA sections representing PKS that produce specifically for PUFA (polyunsaturated fatty acids) are reproduced in vitro. The PUFA PKS-specific amino acid sequence LGIDSIKRVEIL makes it possible to derive oligonucleotides which are used for the efficient PCR screening for PUFA PKS genes or PUFA-producing microorganisms. The inventive method is particularly suitable for the high throughput screening of microorganisms for PUFA PKS genes.
摘要翻译：本发明涉及用于快速和简单地鉴定微生物中PUFA PKS的方法。所述方法的特征在于代表PKS产生的专门用于PUFA（多不饱和脂肪酸）的DNA片段在体外再现。 PUFA PKS特异性氨基酸序列LGIDSIKRVEIL可以获得用于有效PCR筛选PUFA PKS基因或产生PUFA的微生物的寡核苷酸。本发明的方法特别适合用于PUFA PKS基因的微生物的高通量筛选。

9. 发明授权

US08900831B2 Method for the cultivation of microorganisms of the genus thraustochytriales by using an optimized low salt medium 有权
标题翻译：通过使用优化的低盐培养基培养破囊壶菌属微生物的方法
公开(公告)号：US08900831B2
公开(公告)日：2014-12-02
申请号：US10578968
申请日：2004-11-10
申请人： Matthias Rüsing , Markus Luy
发明人： Matthias Rüsing , Markus Luy
IPC分类号： C12P7/64 , C12N1/00
CPC分类号： C12P7/6427 , A23K10/37 , A23L33/12 , A23V2002/00 , C12N1/12 , C12P7/6472
摘要： The invention relates to an optimized method for cultivating microorganisms of the genus Thraustochytriales, according to which the microorganisms are cultivated in a low salt medium without adding sodium salts and chloride salts, the total salt content being less than 3.5 g/L (corresponding to less than 10 percent of sea water salt content), whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention especially relates to novel optimized media having a substantially reduced total salt content, above all a particularly reduced NaCl content. The production of PUFAs can be substantially improved and significantly simplified by using a novel combination of different salts as a media composition in which the overall weight ratios of ions do not exceed 1.75 g/L. Furthermore, said medium preferably contains no added sodium salt and chloride salt at all, which helps prevent environmental damages caused by wastewaters containing salt.
摘要翻译：本发明涉及一种用于培养破囊壶菌属微生物的优化方法，根据该方法，微生物在低盐培养基中培养而不添加钠盐和氯化物盐，总盐含量小于3.5g / L（相当于少于超过10％的海水盐含量），由此从微生物和/或介质中分离PUFA。本发明特别涉及具有显着降低的总盐含量，特别是特别降低的NaCl含量的新型优化的介质。通过使用不同盐的新型组合作为其中离子的总重量比不超过1.75g / L的介质组合物，可以显着改善和显着地简化PUFA的生产。此外，所述介质优选根本不含有添加的钠盐和氯化物盐，这有助于防止由含有盐的废水引起的环境损害。

10. 发明申请

US20070141686A1 Process for cultivating microoraganisms of the genus thraustochytriales 有权
标题翻译：培养破囊壶菌属微阵列反应的方法
公开(公告)号：US20070141686A1
公开(公告)日：2007-06-21
申请号：US10578965
申请日：2004-11-10
申请人： Markus Luy , Matthias Rusing
发明人： Markus Luy , Matthias Rusing
IPC分类号： C12P7/62 , C12N1/21
CPC分类号： C12P7/6427 , C12N1/14 , C12P7/6472
摘要： The invention relates to an optimized method for the production of PUFAs by cultivating microorganisms belonging to the group of Stramenopiles in a fermentation medium that is pH-stabilized using calcium carbonate and comprises 3-15 g/L CaCO3, whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention particularly relates to novel optimized media having a different CaCO3 content. By using adequate quantities of CaCO3, the process can be significantly simplified during fermentation while greater quantities of DHA can be obtained at an increased oil content in the biomass. They allow microorganisms belonging to the Stramenopiles to be fermented without controlling the pH, thereby substantially improving and significantly simplifying PUFA production.
摘要翻译：本发明涉及通过在使用碳酸钙进行pH稳定化的发酵培养基中培养属于Stramenopiles组的微生物并且包含3-15g / L CaCO 3来生产PUFA的优化方法，由此将PUFA从微生物和/或培养基。本发明特别涉及具有不同CaCO3含量的新型优化介质。通过使用足够量的CaCO 3，可以在发酵期间显着简化该过程，同时在生物质中增加的油含量可获得更多量的DHA。它们允许属于Stramenopiles的微生物发酵而不控制pH值，从而显着改善和显着简化PUFA生产。

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