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1. 发明授权

US06448381B1 DNA encoding heparin-binding growth factor 失效
标题翻译：编码肝素结合生长因子的DNA
公开(公告)号：US06448381B1
公开(公告)日：2002-09-10
申请号：US07542232
申请日：1990-06-21
申请人： Thomas F. Deuel , Yue-Sheng Li , Ned R. Siegel , Peter G. Milner
发明人： Thomas F. Deuel , Yue-Sheng Li , Ned R. Siegel , Peter G. Milner
IPC分类号： A61K3824
CPC分类号： C07K14/475
摘要： A novel heparin-binding growth factor of 18.9 kDa mol. wt. is disclosed which is purified from bovine uterus and human placenta and has a unique 25 N-terminal amino acid sequence as follows: Gly-Lys-Lys-Glu-Lys-Pro-Glu-Lys-Lys-Val-Lys-Lys-Ser-Asp-Cys-Gly-Glu-Trp-Gln-Trp-Ser-Val-Cys-Val-Pro.
摘要翻译： 18.9 kDa mol的新型肝素结合生长因子。重量公开了其从牛子宫和人胎盘中纯化，并具有如下独特的25个N-末端氨基酸序列：Gly-Lys-Lys-Glu-Lys-Pro-Glu-Lys-Lys-Val-Lys-Lys-Ser -Asp-Cys-Gly-Glu-Trp-Gln-Trp-Ser-Val-Cys-Val-Pro。

2. 发明授权

US5001064A Phosphatidylinositol 4-kinase 失效
标题翻译：磷脂酰肌醇4激酶
公开(公告)号：US5001064A
公开(公告)日：1991-03-19
申请号：US208429
申请日：1988-06-20
申请人： Thomas F. Deuel
发明人： Thomas F. Deuel
IPC分类号： C12N9/12
CPC分类号： C12N9/12
摘要： A novel phosphatidylinositol 4-kinase isolated from bovine uterus having the following characteristics:(a) molecular weight of about 55 kDa as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis;(b) K.sub.m of about 18 .mu.M for ATP;(c) K.sub.m of about 22 .mu.g/ml for phosphatidylinositol;(d) pH optimum of about 6.0 to 7.0;(e) activated by Mg.sup.2+ ;(f) inhibited by Ca.sup.2+ ;(g) utilizes ATP and 2'-dioxy-ATP as phosphoryl donors and specifically phosphorylates phosphatidylinositol on the 4-position; and(h) essentially free of phosphatidylinositol-4-phosphate 5-kinase activity determined by treatment with phorphoryl donors ADP, GTP, ITP or CTP.
摘要翻译：从牛子宫分离的新型磷脂酰肌醇4-激酶具有以下特征：（a）通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定的约55kDa的分子量; （b）ATP约为18微米的Km; （c）磷脂酰肌醇的Km为约22μg/ ml; （d）最适pH约6.0-7.0; （e）被Mg2 +活化; （f）被Ca2 +抑制; （g）使用ATP和2'-二氧-ATP作为磷酰基供体，并特异性磷酸化磷脂酰肌醇4位; 和（h）基本上不含磷酰肌醇-4-磷酸5-磷酸激酶活性，其通过用佛波酯供体ADP，GTP，ITP或CTP处理而确定。

3. 发明授权

US5770720A Ubiquitin conjugating enzymes having transcriptional repressor activity 失效
标题翻译：具有转录阻遏物活性的泛素缀合酶
公开(公告)号：US5770720A
公开(公告)日：1998-06-23
申请号：US706214
申请日：1996-08-30
申请人： Thomas F. Deuel , Zhao-Yi Wang , Thomas E. Shenk
发明人： Thomas F. Deuel , Zhao-Yi Wang , Thomas E. Shenk
IPC分类号： A61K38/00 , C07K14/11 , C07K14/82 , C12N9/00 , C12N15/12 , C12N15/31 , C12N15/52 , C12N15/85
CPC分类号： C07K14/005 , C07K14/82 , C12N9/93 , A61K38/00 , C07K2319/00 , C12N2760/16022
摘要： A human ubiquitin conjugating enzyme, designated hUBC-9, its full amino acid sequence, and nucleic acid polymers which encode hUBC-9 are disclosed. In addition to having functional ubiquitin conjugating activity, this enzyme has transcriptional repressor activity which is independent of the conjugating activity. The conjugating activity of hUBC-9 enhances transcription through degradation of transcription suppressor proteins such as WT1, and possibly, of hUBC-9 itself. The repressor activity of hUBC-9 suppress gene transcription, probably by disrupting the transcriptional initiation complex through specific interactions with the DNA binding region of the TATA binding protein (TBP). In use, hUBC-9, yUBC-9 and other ubiquitin conjugating enzymes having repressor activity can be fused to proteins having a DNA binding domain, such as Gal4, or used in conjunction with reppressors such as Wilm's tumor suppressor gene product, WT1. Such enzymes and the nucleic acid polymers encoding them can be used for regulating transcription of a target gene in both pharmaceutical and non-pharmaceutical applications.
摘要翻译：公开了一种人泛素缀合酶，命名为hUBC-9，其全部氨基酸序列和编码hUBC-9的核酸聚合物。除了具有功能泛素缀合活性之外，该酶具有独立于缀合活性的转录阻遏物活性。 hUBC-9的共轭活性通过转录抑制蛋白如WT1和可能的hUBC-9本身的降解来增强转录。 hUBC-9的阻遏物活性抑制基因转录，可能通过与TATA结合蛋白（TBP）的DNA结合区的特异性相互作用破坏转录起始复合物。在使用中，具有阻遏物活性的hUBC-9，yUBC-9和其他泛素缀合酶可以与具有DNA结合结构域的蛋白质如Gal4融合，或与抑制因子如Wilm肿瘤抑制基因产物WT1结合使用。这些酶和编码它们的核酸聚合物可用于调节药物和非药物应用中靶基因的转录。

4. 发明授权

US5332669A Prostate-derived mitogen 失效
标题翻译：前列腺素有丝分裂原
公开(公告)号：US5332669A
公开(公告)日：1994-07-26
申请号：US356739
申请日：1989-05-24
申请人： Thomas F. Deuel
发明人： Thomas F. Deuel
IPC分类号： A61K35/55 , A61K38/00 , C07K1/20 , C07K14/00 , C07K14/475 , C12N5/00 , A61K37/36
CPC分类号： C07K14/475 , A61K38/00 , Y10S530/827 , Y10S530/854
摘要： A prostate-derived growth factor is disclosed which has a mol. wt. of about 28 kDa, an NH.sub.2 -terminal sequence substantially identical to the NH.sub.2 -terminal sequence of the 6 kDa mature rat EGF, potent mitogenic activity against NRK cells without additional carboxy terminal processing to the mature 6 kDa EGF molecular species, cross-reacts with antisera against rat EGF and its mitogenic activity is blocked by anti-EGF receptor antisera.
摘要翻译：公开了一种前列腺衍生生长因子，重量约28kDa，与6kDa成熟大鼠EGF的NH2-末端序列基本相同的NH2-末端序列，对NRK细胞有效的促有丝分裂活性，而对成熟6kDa EGF分子种类没有额外的羧基末端加工，与针对大鼠EGF的抗血清及其促有丝分裂活性被抗EGF受体抗血清阻断。

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