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1. 发明申请

US20080161242A1 High pressure treatment of proteins for reduced immunogenicity 审中-公开
标题翻译：高压处理蛋白质降低免疫原性
公开(公告)号：US20080161242A1
公开(公告)日：2008-07-03
申请号：US11901613
申请日：2007-09-17
申请人： Theodore W. Randolph , John F. Carpenter , Richard St. John , Lyndal K. Hesterberg , Matthew B. Seefeldt
发明人： Theodore W. Randolph , John F. Carpenter , Richard St. John , Lyndal K. Hesterberg , Matthew B. Seefeldt
IPC分类号： A61K38/00
CPC分类号： C07K1/1136 , A61K38/1774 , A61K38/215 , A61K38/27
摘要： Protein compositions with reduced immunogenicity are disclosed, as well as methods for producing such compositions.
摘要翻译：公开了具有降低的免疫原性的蛋白质组合物，以及生产这种组合物的方法。

2. 发明授权

US07829681B2 High-pressure inclusion body solubilization and protease clipping of recombinant fusion proteins 有权
标题翻译：重组融合蛋白的高压包涵体溶解和蛋白酶剪切
公开(公告)号：US07829681B2
公开(公告)日：2010-11-09
申请号：US12098877
申请日：2008-04-07
申请人： Matthew B. Seefeldt , Lyndal K. Hesterberg , Theodore W. Randolph , John F. Carpenter
发明人： Matthew B. Seefeldt , Lyndal K. Hesterberg , Theodore W. Randolph , John F. Carpenter
IPC分类号： C07K14/00 , C12N9/48 , C12P21/04
CPC分类号： C07K1/113 , C07K2/00
摘要： Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.
摘要翻译：本文描述了在大于大气压的压力下溶解和蛋白水解切割融合蛋白聚集体（包括自催化融合蛋白）以产生可溶性靶多肽的方法。

3. 发明申请

US20080248522A1 HIGH-PRESSURE INCLUSION BODY SOLUBILIZATION AND PROTEASE CLIPPING OF RECOMBINANT FUSION PROTEINS 有权
标题翻译：重组蛋白的高压包裹体溶解和蛋白质剪切
公开(公告)号：US20080248522A1
公开(公告)日：2008-10-09
申请号：US12098877
申请日：2008-04-07
申请人： Matthew B. SEEFELDT , Lyndal K. Hesterberg , Theodore W. Randolph , John F. Carpenter
发明人： Matthew B. SEEFELDT , Lyndal K. Hesterberg , Theodore W. Randolph , John F. Carpenter
IPC分类号： C12P21/06 , C07K2/00
CPC分类号： C07K1/113 , C07K2/00
摘要： Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.
摘要翻译：本文描述了在大于大气压的压力下溶解和蛋白水解切割融合蛋白聚集体（包括自催化融合蛋白）以产生可溶性靶多肽的方法。

4. 发明授权

US06489450B2 High pressure refolding of protein aggregates and inclusion bodies 有权
标题翻译：蛋白质聚集体和包涵体的高压重折叠
公开(公告)号：US06489450B2
公开(公告)日：2002-12-03
申请号：US09350327
申请日：1999-07-09
申请人： Theodore W. Randolph , John F. Carpenter , Richard St. John
发明人： Theodore W. Randolph , John F. Carpenter , Richard St. John
IPC分类号： A23J100
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译：本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。

5. 发明授权

US08710197B2 High pressure refolding of protein aggregates and inclusion bodies 失效
标题翻译：蛋白质聚集体和包涵体的高压重折叠
公开(公告)号：US08710197B2
公开(公告)日：2014-04-29
申请号：US12940587
申请日：2010-11-05
申请人： Theodore W. Randolph , John F. Carpenter , Richard St. John
发明人： Theodore W. Randolph , John F. Carpenter , Richard St. John
IPC分类号： C07K14/00 , C12N9/00
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译：本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。

6. 发明申请

US20110046357A1 HIGH PRESSURE REFOLDING OF PROTEIN AGGREGATES AND INCLUSION BODIES 失效
标题翻译：蛋白质聚集体和包涵体的高压重塑
公开(公告)号：US20110046357A1
公开(公告)日：2011-02-24
申请号：US12940587
申请日：2010-11-05
申请人： Theodore W. Randolph , John F. Carpenter , Richard St. John
发明人： Theodore W. Randolph , John F. Carpenter , Richard St. John
IPC分类号： C07K1/14
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译：本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。

7. 发明申请

US20100255536A1 HIGH PRESSURE REFOLDING OF PROTEIN AGGREGATES AND INCLUSION BODIES 审中-公开
标题翻译：蛋白质聚集体和包涵体的高压重塑
公开(公告)号：US20100255536A1
公开(公告)日：2010-10-07
申请号：US12819779
申请日：2010-06-21
申请人： Theodore W. RANDOLPH , John F. Carpenter , Richard St. John
发明人： Theodore W. RANDOLPH , John F. Carpenter , Richard St. John
IPC分类号： C12P21/00 , C07K14/61
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译：本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。

8. 发明授权

US07064192B2 High pressure refolding of protein aggregates and inclusion bodies 有权
公开(公告)号：US07064192B2
公开(公告)日：2006-06-20
申请号：US10292692
申请日：2002-11-12
申请人： Theodore W. Randolph , John F. Carpenter , Richard St. John
发明人： Theodore W. Randolph , John F. Carpenter , Richard St. John
IPC分类号： C07K1/02 , C12N9/00
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.

9. 发明授权

US07767795B2 High pressure refolding of protein aggregates and inclusion bodies 有权
标题翻译：蛋白质聚集体和包涵体的高压重折叠
公开(公告)号：US07767795B2
公开(公告)日：2010-08-03
申请号：US11341013
申请日：2006-01-27
申请人： Theodore W. Randolph , John F. Carpenter , Richard St. John
发明人： Theodore W. Randolph , John F. Carpenter , Richard St. John
IPC分类号： C07K14/00 , C12N9/00
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译：本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。

10. 发明申请

US20110243996A1 Spray freeze dry of compositions for intranasal administration 审中-公开
标题翻译：用于鼻内给药的组合物喷雾冷冻干燥
公开(公告)号：US20110243996A1
公开(公告)日：2011-10-06
申请号：US13065965
申请日：2011-04-01
申请人： Vu Truong-Le , Binh V. Pham , John F. Carpenter , Robert Seid , Theodore W. Randolph
发明人： Vu Truong-Le , Binh V. Pham , John F. Carpenter , Robert Seid , Theodore W. Randolph
IPC分类号： A61K35/76 , A61K9/14
CPC分类号： A61K9/0043 , A61K9/1617 , A61K9/1623 , A61K9/1658 , A61K9/1694
摘要： This invention provides methods and compositions to preserve bioactive materials, such as peptides, nucleic acids, viruses, bacteria, cells, or liposomes, in freeze dried particles suitable for intranasal administration. Methods provide spray freeze drying of formulations to form stable freeze dried particles for intranasal administration.
摘要翻译：本发明提供在适于鼻内给药的冷冻干燥颗粒中保存生物活性物质如肽，核酸，病毒，细菌，细胞或脂质体的方法和组合物。方法提供制剂的喷雾冷冻干燥以形成用于鼻内给药的稳定的冷冻干燥颗粒。

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