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1. 发明授权

US06808910B2 Polyhydroxyalkanoate synthase and gene encoding the same enzyme 失效
标题翻译：聚羟基链烷酸酯合成酶和编码相同酶的基因
公开(公告)号：US06808910B2
公开(公告)日：2004-10-26
申请号：US10266787
申请日：2002-10-09
申请人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
发明人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
IPC分类号： C12N902
CPC分类号： C12P7/625 , C12N9/1025
摘要： A novel polyhydroxyalkanoate (PHA) synthase derived from a microorganism capable of producing a PHA having a novel side-chain structure and a DNA encoding the amino acid sequence for the synthase are provided. Two PHA synthase proteins (SEQ ID Nos. 1 and 3) derived from Pseudomonas jessenii P161 (FERM BP-7376) and PHA synthase genes encoding these PHA synthases are provided, respectively (SEQ ID Nos. 2 and 4). A recombinant microorganism is endowed with a PHA producing ability.
摘要翻译：提供了源自能够产生具有新型侧链结构的PHA的微生物和编码合成酶的氨基酸序列的DNA的新型聚羟基链烷酸酯（PHA）合酶。分别提供了两种衍生自琼脂糖假单胞菌P161（FERM BP-7376）的PHA合酶蛋白（SEQ ID No.1和3）和编码这些PHA合成酶的PHA合酶基因（SEQ ID No.2和4）。重组微生物具有PHA产生能力。

2. 发明授权

US06492147B2 Method for producing microbial polyester 失效
标题翻译：生产微生物聚酯的方法
公开(公告)号：US06492147B2
公开(公告)日：2002-12-10
申请号：US09740021
申请日：2000-12-20
申请人： Takeshi Imamura , Tetsuya Yano , Shin Kobayashi , Sakae Suda , Tsutomu Honma
发明人： Takeshi Imamura , Tetsuya Yano , Shin Kobayashi , Sakae Suda , Tsutomu Honma
IPC分类号： C12P762
CPC分类号： C08G63/06 , C12P7/625 , Y10S435/874 , Y10S435/875 , Y10S435/876 , Y10S435/877
摘要： A method for producing a microbial polyester by culturing a microorganism being capable of producing a poly hydroxyalkanoate polyester in a culture medium containing 1-hexene as a sole carbon source.
摘要翻译：通过培养能够在含有1-己烯作为唯一碳源的培养基中生产聚羟基链烷酸酯聚酯的微生物来生产微生物聚酯的方法。

3. 发明授权

US06586562B2 Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same 失效
标题翻译：聚羟基链烷酸酯，其制备方法和用于其的微生物
公开(公告)号：US06586562B2
公开(公告)日：2003-07-01
申请号：US09748205
申请日：2000-12-27
申请人： Tsutomu Honma , Toyoko Kobayashi , Tetsuya Yano , Shin Kobayashi , Takeshi Imamura , Sakae Suda , Takashi Kenmoku
发明人： Tsutomu Honma , Toyoko Kobayashi , Tetsuya Yano , Shin Kobayashi , Takeshi Imamura , Sakae Suda , Takashi Kenmoku
IPC分类号： C08G6306
CPC分类号： C12P7/62 , C08G63/06 , C08G63/6822 , C08G63/6852 , Y10S435/874
摘要： A polyhydroxyalkanoate having a monomer unit composition represented by General Formula (1): AmB(1−m) (1) wherein A is represented by General Formula (2), B is at least one selected from the group consisting of monomer units represented by General Formula (3) or (4), and m has a value of 0.01 or larger and smaller than 1: wherein n has a value of 0 to 10, k has a value of 3 or 5, and R is at least one group selected from the group consisting of groups represented by General Formulae (5) to (7): in Formula (5) R1 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and q is an integer selected from 1 to 8; in Formula (6) R2 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and r is an integer selected from 1 to 8; in Formula (7) R3 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and s is an integer selected from 1 to 8. The efficient production methods are also provided.
摘要翻译：具有由通式（1）表示的单体单元组成的聚羟基链烷酸酯：其中A由通式（2）表示，B为选自由通式（3）或（4）表示的单体单元中的至少一种，，m的值为0.01以上且小于1，其中n为0〜10，k为3或5，R为选自由通式（5）〜（7）中，式（5）中，R 1为选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团。 q为选自1至8的整数; 在式（6）中，R 2是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团; 并且r是选自1至8的整数; 在式（7）中，R 3是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团; s是选自1至8的整数。还提供了有效的制备方法。

4. 发明授权

US07169598B2 Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same 失效
标题翻译：聚羟基链烷酸酯，其制备方法和用于其的微生物
公开(公告)号：US07169598B2
公开(公告)日：2007-01-30
申请号：US10649646
申请日：2003-08-28
申请人： Tsutomu Honma , Toyoko Kobayashi , Tetsuya Yano , Shin Kobayashi , Takeshi Imamura , Sakae Suda , Takashi Kenmoku
发明人： Tsutomu Honma , Toyoko Kobayashi , Tetsuya Yano , Shin Kobayashi , Takeshi Imamura , Sakae Suda , Takashi Kenmoku
IPC分类号： C12N1/20
CPC分类号： C12P7/62 , C08G63/06 , C08G63/6822 , C08G63/6852 , Y10S435/874
摘要： A polyhydroxyalkanoate having a monomer unit composition represented by General Formula (1): AmB(1-m) (1) wherein A is represented by General Formula (2), B is at least one selected from the group consisting of monomer units represented by General Formula (3) or (4), and m has a value of 0.01 or larger and smaller than 1: wherein n has a value of 0 to 10, k has a value of 3 or 5, and R is at least one group selected from the group consisting of groups represented by General Formulae (5) to (7): in Formula (5) R1 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5. and —C3F7; and q is an integer selected from 1 to 8; in Formula (6) R2 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and r is an integer selected from 1 to 8; in Formula (7) R3 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and s is an integer selected from 1 to 8. The efficient production methods are also provided.
摘要翻译：具有由通式（1）表示的单体单元组成的聚羟基链烷酸酯：<？in-line-formula description =“In-line Formulas”end =“lead”？> A （1-m）（1）<？in-line-formula description =“In-line Formulas”end =“tail”？>其中A由通式（2）表示，B为至少一个选自由通式（3）或（4）表示的单体单元组，并且m具有0.01以上且小于1的值：其中n具有0至10的值，k的值为3 或5，并且R是选自由通式（5）至（7）表示的基团中的至少一个基团：在式（5）中，R 1是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 H 5。和-C 3 N 3 R 7; q为选自1至8的整数; 在式（6）中，R 2是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3， C 2 F 5和-C 3 F 7; 并且r是选自1至8的整数; 在式（7）中，R 3是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3 N， C 2 F 5和-C 3 F 7; s是选自1至8的整数。还提供了有效的制备方法。

5. 发明授权

US07101696B2 Polyhydroxyalkanoate synthase and gene encoding the same enzyme 失效
标题翻译：聚羟基链烷酸酯合成酶和编码相同酶的基因
公开(公告)号：US07101696B2
公开(公告)日：2006-09-05
申请号：US10914244
申请日：2004-08-10
申请人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
发明人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
IPC分类号： C12N9/10 , C12N1/20 , C12N15/00 , C12P21/06 , C07H21/04 , C12Q1/48 , C12Q1/00
CPC分类号： C12P7/625 , C12N9/1025
摘要： A novel polyhydroxyalkanoate (PHA) synthase derived from a microorganism capable of producing a PHA having a novel side-chain structure and a DNA encoding the amino acid sequence for the synthase are provided. Two PHA synthase proteins (SEQ ID NOs: 1 and 3) derived from Pseudomonas cichorii H45 (FERM BP-7374) and PHA synthase genes encoding these PHA synthases are provided, respectively (SEQ ID NOs: 2 and 4). A recombinant microorganism is endowed with a PHA producing ability.
摘要翻译：提供了源自能够产生具有新型侧链结构的PHA的微生物和编码合成酶的氨基酸序列的DNA的新型聚羟基链烷酸酯（PHA）合酶。分别提供了两种源自Pseudomonas cichorii H45（FERM BP-7374）的PHA合酶蛋白（SEQ ID NO：1和3）和编码这些PHA合成酶的PHA合酶基因（SEQ ID NO：2和4）。重组微生物具有PHA产生能力。

6. 发明申请

US20050009157A1 Polyhydroxyalkanoate synthase and gene encoding the same enzyme 失效
标题翻译：聚羟基链烷酸酯合成酶和编码相同酶的基因
公开(公告)号：US20050009157A1
公开(公告)日：2005-01-13
申请号：US10914244
申请日：2004-08-10
申请人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
发明人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
IPC分类号： C12N9/10 , C12P7/62 , C12N1/20 , C12N9/18
CPC分类号： C12P7/625 , C12N9/1025
摘要： A novel polyhydroxyalkanoate (PHA) synthase derived from a microorganism capable of producing a PHA having a novel side-chain structure and a DNA encoding the amino acid sequence for the synthase are provided. Two PHA synthase proteins (SEQ ID NOs: 1 and 3) derived from Pseudomonas cichorii H45 (FERM BP-7374) and PHA synthase genes encoding these PHA synthases are provided, respectively (SEQ ID NOs: 2 and 4). A recombinant microorganism is endowed with a PHA producing ability.
摘要翻译：提供了源自能够产生具有新型侧链结构的PHA的微生物和编码合成酶的氨基酸序列的DNA的新型聚羟基链烷酸酯（PHA）合酶。分别提供了两种源自Pseudomonas cichorii H45（FERM BP-7374）的PHA合酶蛋白（SEQ ID NO：1和3）和编码这些PHA合成酶的PHA合酶基因（SEQ ID NO：2和4）。重组微生物具有PHA产生能力。

7. 发明授权

US06812013B2 Polyhydroxyalkanoate synthase and gene encoding the same 失效
标题翻译：聚羟基链烷酸酯合成酶和编码基因的基因
公开(公告)号：US06812013B2
公开(公告)日：2004-11-02
申请号：US10245765
申请日：2002-09-18
申请人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
发明人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
IPC分类号： C12N902
CPC分类号： C12N9/1025 , C12P7/625
摘要： The present invention provides a PHA (polyhydroxyalkanoate) synthase useful in a process for preparing a PHA, a gene encoding the enzyme, a recombinant vector comprising the gene, a transformant transformed by the vector, a process for producing a PHA synthase utilizing the transformant and a process for preparing a PHA utilizing the transformant. A transformant obtained by introducing a PHA synthase gene from Pseudomonas putida P91 strain into a host microorganism is cultured to produce a PHA synthase or PHA.
摘要翻译：本发明提供了可用于制备PHA的方法中的PHA（聚羟基链烷酸酯）合成酶，编码该酶的基因，包含该基因的重组载体，由该载体转化的转化体，利用该转化体产生PHA合成酶的方法和使用转化体制备PHA的方法。将通过将恶臭假单胞菌P91菌株的PHA合成酶基因导入宿主微生物而得到的转化体进行培养，生成PHA合成酶或PHA。

8. 发明授权

US06951747B2 Polyhydroxyalkanoate synthase and gene encoding the same 失效
标题翻译：聚羟基链烷酸酯合成酶和编码基因的基因
公开(公告)号：US06951747B2
公开(公告)日：2005-10-04
申请号：US10914242
申请日：2004-08-10
申请人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
发明人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
IPC分类号： C12N1/21 , C12N9/10 , C12P7/62 , C07H21/04 , C12N1/20 , C12N15/00 , C12P21/06
CPC分类号： C12N9/1025 , C12P7/625
摘要： The present invention provides a PHA (polyhydroxyalkanoate) synthase useful in a process for preparing a PHA, a gene encoding the enzyme, a recombinant vector comprising the gene, a transformant transformed by the vector, a process for producing a PHA synthase utilizing the transformant and a process for preparing a PHA utilizing the transformant. A transformant obtained by introducing a PHA synthase gene from Pseudomonas putida P91 strain into a host microorganism is cultured to produce a PHA synthase or PHA.
摘要翻译：本发明提供了可用于制备PHA的方法中的PHA（聚羟基链烷酸酯）合成酶，编码该酶的基因，包含该基因的重组载体，由该载体转化的转化体，利用该转化体产生PHA合成酶的方法和使用转化体制备PHA的方法。培养通过将恶臭假单胞菌P91菌株的PHA合成酶基因导入宿主微生物而得到的转化体，生成PHA合成酶或PHA。

9. 发明申请

US20050009155A1 Polyhydroxyalkanoate synthase and gene encoding the same enzyme 失效
公开(公告)号：US20050009155A1
公开(公告)日：2005-01-13
申请号：US10914187
申请日：2004-08-10
申请人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
发明人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
IPC分类号： C12N1/21 , C12N9/10 , C12P7/62 , C07H21/04 , C12N9/04 , C12N9/18
CPC分类号： C12N9/1025 , C12P7/625
摘要： A novel polyhydroxyalkanoate (PHA) synthase derived from a microorganism capable of producing a PHA having a novel side-chain structure and a DNA encoding the amino acid sequence for the synthase are provided. Two PHA synthase proteins (SEQ ID NOs: 1 and 3) derived from Pseudomonas cichorii YN2 (FERM BP-7375) and PHA synthase genes encoding these PHA synthases are provided, respectively (SEQ ID NOs: 2 and 4). A recombinant microorganism is endowed with a PHA producing ability.

10. 发明授权

US06803220B2 Polyhydroxyalkanoate synthase and gene encoding the same enzyme 失效
标题翻译：聚羟基链烷酸酯合成酶和编码相同酶的基因
公开(公告)号：US06803220B2
公开(公告)日：2004-10-12
申请号：US10259632
申请日：2002-09-30
申请人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
发明人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
IPC分类号： C12N902
CPC分类号： C12P7/625 , C12N9/1025
摘要： A novel polyhydroxyalkanoate (PHA) synthase derived from a microorganism capable of producing a PHA having a novel side-chain structure and a DNA encoding the amino acid sequence for the synthase are provided. Two PHA synthase proteins (SEQ ID NOs. 1 and 3) derived from Pseudomonas cichorii H45 (FERM BP-7374) and PHA synthase genes encoding these PHA synthases are provided, respectively (SEQ ID NOs. 2 and 4). A recombinant microorganism is endowed with a PHA producing ability.
摘要翻译：提供了源自能够产生具有新型侧链结构的PHA的微生物和编码合成酶的氨基酸序列的DNA的新型聚羟基链烷酸酯（PHA）合酶。分别提供了两种衍生自Cseudomonas cichorii H45（FERM BP-7374）的PHA合成酶蛋白（SEQ ID NO.1和3）和编码这些PHA合成酶的PHA合酶基因（SEQ ID NO.2和4）。重组微生物具有PHA产生能力。

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