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1. 发明授权

US06641526B1 Development of normal offspring from oocytes injected with freeze-dried spermatozoa 有权
标题翻译：从注射冷冻干燥精子的卵母细胞培养正常后代
公开(公告)号：US06641526B1
公开(公告)日：2003-11-04
申请号：US09177391
申请日：1998-10-23
申请人： Teruhiko Wakayama , Ryuzo Yanagimachi
发明人： Teruhiko Wakayama , Ryuzo Yanagimachi
IPC分类号： A61B1743
CPC分类号： A01N1/02 , A01N1/0284
摘要： The invention provides a method for freeze-drying spermatozoa to obtain at least one reconstituted spermatozoon whose head (nucleus) is capable of fertilizing an oocyte to produce a live offspring. The motility of spermatozoa which have been freeze-dried and stored in a vacuum at room temperature is not restored when rehydrated. Their plasma membranes are disrupted and they are all “dead” in the conventional sense. However, when they are injected microsurgically into oocytes, their nuclei transform into male pronuclei and participate in normal embryonic development.
摘要翻译：本发明提供了一种用于冷冻精子的方法，以获得至少一个重构的精子，其头（核）能够施用卵母细胞以产生活的后代。冷冻干燥并在室温真空中储存的精子的运动性在再水合时不会恢复。他们的等离子体膜被破坏，它们在常规意义上都是“死的”。然而，当它们被显微外科注射到卵母细胞中时，它们的核转变成雄性原核并参与正常的胚胎发育。

2. 发明授权

US6143564A Use of the polar body chromosomes for the production of embryos and normal offspring 失效
标题翻译：使用极体染色体来生产胚胎和正常的后代
公开(公告)号：US6143564A
公开(公告)日：2000-11-07
申请号：US227365
申请日：1999-01-08
申请人： Teruhiko Wakayama , Ryuzo Yanagimachi
发明人： Teruhiko Wakayama , Ryuzo Yanagimachi
IPC分类号： C12N15/877 , C12N15/00 , A01K67/00 , A01K67/027 , C12N15/02 , C12N15/63
CPC分类号： C12N15/8775
摘要： The invention provides a method for obtaining a live offspring having maternal chromosomes derived from a live first polar body of an oocyte that has completed the first meiotic division. It has been discovered herein that chromosomes in the first polar body are able to participate in normal embryonic development if they are allowed to complete the second meiotic division within an enucleated mature oocyte, and are then allowed to mingle with chromosomes of a spermatozoon. The invention further provides a method for producing up to four embyros or live offspring having the chromosomes of a single oocyte, by using both the first polar body chromosomes, and the second polar body chromosomes to reconstitute recipient enucleated (fertilized) oocytes.
摘要翻译：本发明提供了一种获得具有源自卵母细胞的活的第一极体的母体染色体的活后代的方法，其完成了第一次减数分裂。在本文中已经发现，如果允许其在去核的成熟卵母细胞内完成第二次减数分裂，则第一极体中的染色体能够参与正常的胚胎发育，然后允许其与精子的染色体相互融合。本发明还提供了通过使用第一极体染色体和第二极体染色体来重构受体去核（受精）卵母细胞来生产多达四个具有单个卵母细胞染色体的雌雄或活后代的方法。

3. 发明授权

US06331659B1 Cumulus cells as nuclear donors 有权
标题翻译：积细胞作为核供体
公开(公告)号：US06331659B1
公开(公告)日：2001-12-18
申请号：US09132104
申请日：1998-08-10
申请人： Teruhiko Wakayama , Ryuzo Yanagimachi
发明人： Teruhiko Wakayama , Ryuzo Yanagimachi
IPC分类号： C12N1500
CPC分类号： C12N15/8775
摘要： Animals are produced following injection of adult somatic cell nuclei into enucleated oocytes. The invention provides a method for cloning an animal by directly inserting at least a portion of the adult somatic nucleus (including the minimum chromosomal material able to support development) into a recipient enucleated oocyte. Preferably, the nucleus is inserted by microinjection and, more preferably, by piezo electrically-actuated microinjection. The oocyte is activated prior to, during, or up to about 6 hours after insertion of the nucleus, by electroactivation or exposure to a chemical activating agent, such as Sr2+. The activated renucleated oocyte is allowed to develop into an embryo and is transplanted to a host surrogate mother to develop into a live offspring.
摘要翻译：在将成体体细胞核注射到去核卵母细胞后产生动物。本发明提供了一种用于通过将至少一部分成体体细胞核（包括能够支持发育的最小染色体材料）直接插入受体去核卵母细胞来克隆动物的方法。优选地，通过显微注射插入细胞核，更优选地，通过压电电致动显微注射。卵母细胞在插入核后之前，期间或至多约6小时期间被激活，通过电活化或暴露于化学活化剂如Sr2 +。将激活的复核卵母细胞发育成胚胎，并移植到宿主代孕母体中以形成活的后代。

4. 发明申请

US20090126032A1 Method to produce cloned embryos and adults from cultured cells 审中-公开
标题翻译：从培养细胞产生克隆的胚胎和成体的方法
公开(公告)号：US20090126032A1
公开(公告)日：2009-05-14
申请号：US12221060
申请日：2008-07-29
申请人： Anthony C.F. Perry , Peter Mombaerts , Teruhiko Wakayama
发明人： Anthony C.F. Perry , Peter Mombaerts , Teruhiko Wakayama
IPC分类号： A01K67/033 , C12N15/89 , C12N5/00
CPC分类号： C12N15/89 , A01K2227/105 , C12N15/873 , C12N15/8775
摘要： A nuclear transfer method is provided wherein nuclear DNA in whole or part is injected into enucleated oocytes. The method is suitable for different donor cells, and preferably ES cells.
摘要翻译：提供核转移方法，其中将全部或部分核DNA注射到去核卵母细胞中。该方法适用于不同的供体细胞，优选ES细胞。

5. 发明申请

US20090178150A1 Novel Method for Generating Non-Human ES Animals 审中-公开
标题翻译：用于产生非人类ES动物的新方法
公开(公告)号：US20090178150A1
公开(公告)日：2009-07-09
申请号：US12348563
申请日：2009-01-05
申请人： Hiroshi OHTA , Yuko Sakaide , Kazuo Yamagata , Teruhiko Wakayama
发明人： Hiroshi OHTA , Yuko Sakaide , Kazuo Yamagata , Teruhiko Wakayama
IPC分类号： C12N15/87 , A01K67/027
CPC分类号： A01K67/0271 , A01K2227/105 , C12N15/873
摘要： The present invention provides a method for generating non-human animals by transferring ES cells to three or four tetraploid embryos to produce chimeric embryos and implanting the chimeric embryos to a psudopregnant non-human animal.
摘要翻译：本发明提供了一种用于通过将ES细胞转移到三个或四个四倍体胚胎以产生嵌合胚胎并将嵌合胚胎植入诱导性非人类动物来产生非人动物的方法。

6. 发明申请

US20090055945A1 Method for Producing Nuclear-Transplanted Egg 审中-公开
标题翻译：生产核移植蛋的方法
公开(公告)号：US20090055945A1
公开(公告)日：2009-02-26
申请号：US11992886
申请日：2006-09-28
申请人： Satoshi Kishigami , Teruhiko Wakayama , Kazuhiro Saeki
发明人： Satoshi Kishigami , Teruhiko Wakayama , Kazuhiro Saeki
IPC分类号： C12N15/87 , C12N5/00
CPC分类号： C12N15/8775 , C12N5/0609 , C12N15/8771 , C12N2517/04
摘要： It is intended to provide a method for improving a development rate in a somatic nuclear transplantation technique or an artificial insemination technique using a spermatid. The method is a method for producing a nuclear-transplanted egg comprising the steps of transplanting a nucleus of a donor cell into an egg, and treating the nuclear-transplanted egg with an anti-methylating agent.
摘要翻译：旨在提供一种用于提高体细胞核移植技术或使用精子细胞的人工授精技术的发展速度的方法。该方法是生产核移植卵的方法，包括以下步骤：将供体细胞的细胞核移植到卵中，并用抗甲基化剂处理核移植的卵。

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