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1. 发明申请

US20130150599A1 Production of DHA and Other LC-PUFAs in Plants 审中-公开
标题翻译：在植物中生产DHA和其他LC-PUFA
公开(公告)号：US20130150599A1
公开(公告)日：2013-06-13
申请号：US13698412
申请日：2011-05-17
申请人： Terence A. Walsh , Ann Owens Merlo , Daniel Gachotte , Paul Gordon Roessler , Scott Bevan , Jerry M. Kuner , James G. Metz
发明人： Terence A. Walsh , Ann Owens Merlo , Daniel Gachotte , Paul Gordon Roessler , Scott Bevan , Jerry M. Kuner , James G. Metz
IPC分类号： C12N15/82
CPC分类号： C12N15/8247 , C12N9/1029 , C12N9/1288 , C12P7/6427
摘要： The invention provides recombinant host organisms (e.g., plants) genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins (e.g., PPTase and/or ACoAS) that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms (e.g., to obtain PUFAs) as well as products obtained from such organisms (e.g., oil and/or seed).
摘要翻译：本发明提供了用多不饱和脂肪酸（PUFA）合酶系统和一种或多种辅助蛋白（例如，PPT酶和/或ACoAS）进行遗传修饰的重组宿主生物（例如，植物），其允许和/或改善PUFA的生产宿主生物体。本发明还涉及制备和使用这些生物体（例如获得PUFA）以及从这些生物（例如油和/或种子）获得的产品的方法。

2. 发明申请

US20100024050A1 PUFA POLYKETIDE SYNTHASE SYSTEMS AND USES THEREOF 有权
标题翻译：多不饱和脂多糖合成酶系统及其用途
公开(公告)号：US20100024050A1
公开(公告)日：2010-01-28
申请号：US11777277
申请日：2007-07-12
申请人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
发明人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
IPC分类号： A01K67/027 , C12N15/11 , C12N15/00 , C12N1/21 , A01H5/00 , C12Q1/02 , C12P7/64 , C12N15/82 , C12N15/87
CPC分类号： C12N15/52 , C07K14/28 , C07K14/405 , C12N15/8247 , C12N15/8273 , C12P7/625 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
摘要翻译：本发明通常涉及从分离自非细菌生物体或衍生自非细菌生物体的多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物，分离的核酸分子和编码这种PUFA PKS的生物活性结构域的重组核酸分子系统，涉及包含PUFA PKS系统的转基因生物，制备和使用此类体系用于生产感兴趣的生物活性分子的方法，以及鉴定具有这种PUFA PKS系统的新细菌和非细菌微生物的新方法。

3. 发明授权

US07585659B2 Carotene synthase gene and uses therefor 有权
公开(公告)号：US07585659B2
公开(公告)日：2009-09-08
申请号：US11557783
申请日：2006-11-08
申请人： Craig A. Weaver , James G. Metz , Jerry M. Kuner , Frank H. Overton, Jr.
发明人： Craig A. Weaver , James G. Metz , Jerry M. Kuner , Frank H. Overton, Jr.
IPC分类号： C12N1/20 , C12P7/64 , C07C53/00
CPC分类号： C12N9/001 , C12N9/00 , C12N9/0004
摘要： Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities: phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof or methods of producing microorganisms and lipid products lacking pigmentation using the knowledge of the carotene synthase described herein.

4. 发明授权

US07259295B2 PUFA polyketide synthase systems and uses thereof 有权
标题翻译： PUFA聚酮化合物系统及其用途
公开(公告)号：US07259295B2
公开(公告)日：2007-08-21
申请号：US11087100
申请日：2005-03-21
申请人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
发明人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
IPC分类号： A01H1/00 , A01H5/00 , C12P7/64
CPC分类号： C12N15/52 , C07K14/28 , C07K14/405 , C12N15/8247 , C12N15/8273 , C12P7/625 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
摘要翻译：本发明通常涉及从分离自非细菌生物体或衍生自非细菌生物体的多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物，分离的核酸分子和编码这种PUFA PKS的生物活性结构域的重组核酸分子系统，涉及包含PUFA PKS系统的转基因生物，制备和使用此类体系用于生产感兴趣的生物活性分子的方法，以及鉴定具有这种PUFA PKS系统的新细菌和非细菌微生物的新方法。

5. 发明授权

US08426686B2 Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems 有权
标题翻译：使用PUFA聚酮化合物系统在异源生物体中产生多不饱和脂肪酸
公开(公告)号：US08426686B2
公开(公告)日：2013-04-23
申请号：US12796100
申请日：2010-06-08
申请人： James G. Metz , Jerry M. Kuner , James Casey Lippmeier
发明人： James G. Metz , Jerry M. Kuner , James Casey Lippmeier
IPC分类号： A01H5/00 , A01H1/00 , C12N15/82 , C12N1/20 , C07H21/04
CPC分类号： C12N9/1029 , C12N9/93 , C12N15/8247 , C12P7/6427 , C12P7/6472 , Y02P20/52
摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.
摘要翻译：公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用一样。还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物体表达的脂肪酸合酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加生物体中丙二酰辅酶A的水平，以及一方面抑制KASII或KASIII。除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

6. 发明授权

US07960524B2 PUFA polyketide synthase systems and uses thereof 有权
标题翻译： PUFA聚酮化合物系统及其用途
公开(公告)号：US07960524B2
公开(公告)日：2011-06-14
申请号：US11689596
申请日：2007-03-22
申请人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
发明人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
IPC分类号： C07H21/02 , C12N1/12 , C12N15/87 , C12P7/64 , A01H9/00
CPC分类号： C12N15/52 , C07K14/28 , C07K14/405 , C12N15/8247 , C12N15/8273 , C12P7/625 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
摘要翻译：本发明通常涉及从分离自非细菌生物体或衍生自非细菌生物体的多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物，分离的核酸分子和编码这种PUFA PKS的生物活性结构域的重组核酸分子系统，涉及包含PUFA PKS系统的转基因生物，制备和使用此类体系用于生产感兴趣的生物活性分子的方法，以及鉴定具有这种PUFA PKS系统的新细菌和非细菌微生物的新方法。

7. 发明授权

US07919320B2 PUFA polyketide synthase systems and uses thereof 有权
公开(公告)号：US07919320B2
公开(公告)日：2011-04-05
申请号：US11777277
申请日：2007-07-12
申请人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
发明人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
IPC分类号： C12N5/02 , C12N1/00 , C12N15/00 , C12N15/82 , C07H21/04 , A01H9/00
CPC分类号： C12N15/52 , C07K14/28 , C07K14/405 , C12N15/8247 , C12N15/8273 , C12P7/625 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

8. 发明授权

US07759548B2 Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems 有权
标题翻译：使用PUFA聚酮化合物系统在异源生物体中产生多不饱和脂肪酸
公开(公告)号：US07759548B2
公开(公告)日：2010-07-20
申请号：US11686856
申请日：2007-03-15
申请人： James G. Metz , Jerry M. Kuner , James Casey Lippmeier
发明人： James G. Metz , Jerry M. Kuner , James Casey Lippmeier
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04 , C12N1/20 , C12N5/10
CPC分类号： C12N9/1029 , C12N9/93 , C12N15/8247 , C12P7/6427 , C12P7/6472 , Y02P20/52
摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.
摘要翻译：公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用一样。还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物体表达的脂肪酸合酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加生物体中丙二酰辅酶A的水平，以及一方面抑制KASII或KASIII。除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

9. 发明授权

US07605245B2 PUFA polyketide synthase systems and uses thereof 有权
公开(公告)号：US07605245B2
公开(公告)日：2009-10-20
申请号：US11777279
申请日：2007-07-12
申请人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
发明人： James G. Metz , James H. Flatt , Jerry M. Kuner , William R. Barclay
IPC分类号： C12N15/52 , C12N15/53 , C12N15/74 , C12N15/79 , C12N15/82 , C12P7/64
CPC分类号： C12N15/52 , C07K14/28 , C07K14/405 , C12N15/8247 , C12N15/8273 , C12P7/625 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

10. 发明授权

US07582451B2 Carotene synthase gene and uses therefor 有权
标题翻译：胡萝卜素合酶基因及其用途
公开(公告)号：US07582451B2
公开(公告)日：2009-09-01
申请号：US11557761
申请日：2006-11-08
申请人： Craig A. Weaver , James G. Metz , Jerry M. Kuner , Frank H. Overton, Jr.
发明人： Craig A. Weaver , James G. Metz , Jerry M. Kuner , Frank H. Overton, Jr.
IPC分类号： C12P19/00 , C12P7/64 , A01H5/00
CPC分类号： C12N9/001 , C12N9/00 , C12N9/0004
摘要： Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities: phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof or methods of producing microorganisms and lipid products lacking pigmentation using the knowledge of the carotene synthase described herein.
摘要翻译：本文描述了来自裂殖酵母（Schizochytrium）的新型三结构域基因，表示为胡萝卜素合成酶，其编码具有三种不同酶活性的蛋白质：芸苔属脱氢酶（PD），八氢番茄红素合成酶（PS）和番茄红素环化酶（LC）。还描述了编码胡萝卜素合成酶，其同系物，由该基因编码的酶，生物活性部分及其同源物，经遗传修饰以增加或减少该基因的作用的重组核酸分子，微生物和植物的分离基因以及生产类胡萝卜素及其衍生物的方法或使用本文所述的胡萝卜素合酶的知识来生产缺乏色素沉淀的微生物和脂质产物的方法。

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