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    • 1. 发明申请
    • METHOD FOR DETERMINING ANTAGONIST ACTIVITY TO A CYTOKININ RECEPTOR
    • 用于确定对CYTOKININ受体的拮抗剂活性的方法
    • US20110014627A1
    • 2011-01-20
    • US12773456
    • 2010-05-04
    • Tatsuo KAKIMOTOMasayuki HiguchiTsutomu Inoue
    • Tatsuo KAKIMOTOMasayuki HiguchiTsutomu Inoue
    • G01N33/53
    • C07K14/415C12N15/8295
    • The present invention provides a method for analyzing agonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and, a method for analyzing antagonist activity to a cytokinin receptor, which comprises (1) bringing an examinee substance and a substance having agonist-activity to the cytokinin receptor into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and the like.
    • 本发明提供了一种分析激动剂对细胞分裂素受体的活性的方法,其包括(1)使受检物质与导入细胞分裂素受体的DNA的转化细胞接触,(2)测量存在或数量 由细胞分裂素受体表达的细胞分裂素受体的细胞内信号转导和分析拮抗剂对细胞分裂素受体的活性的方法,该方法包括:(1)将受检物质和具有激动剂活性的物质引入细胞分裂素受体与 导入了编码细胞分裂素受体的DNA的转化细胞,(2)测定在转化细胞中表达的细胞分裂素受体的细胞内信号转导的存在或数量等。