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1. 发明申请

US20130122517A1 METHOD OF PERFORMING AN ASSAY 审中-公开
标题翻译：执行测定方法
公开(公告)号：US20130122517A1
公开(公告)日：2013-05-16
申请号：US13811743
申请日：2011-06-30
申请人： Tanja Henzler , Karl Holschuh , Johann Bauer , Thomas Herget , Michael Hartmann , Oliver Poetz , Thomas Joos
发明人： Tanja Henzler , Karl Holschuh , Johann Bauer , Thomas Herget , Michael Hartmann , Oliver Poetz , Thomas Joos
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , B82Y15/00 , G01N33/54346 , G01N33/588
摘要： The present invention relates to a method and kit for performing assays like immunoassays. The assays are performed by using two different types of magnetic beads.
摘要翻译：本发明涉及用于进行如免疫测定法的测定的方法和试剂盒。通过使用两种不同类型的磁珠来进行测定。

2. 发明申请

US20100331199A1 METHOD FOR THE DETECTION AND/OR ENRICHMENT OF ANALYTE PROTEINS AND/OR ANALYTE PEPTIDES FROM A COMPLEX PROTEIN MIXTURE 审中-公开
标题翻译：检测和/或分析分析蛋白和/或从复合蛋白混合物分析肽的方法
公开(公告)号：US20100331199A1
公开(公告)日：2010-12-30
申请号：US12294854
申请日：2007-03-29
申请人： Dieter Stoll , Thomas Joos , Markus Templin , Oliver Poetz
发明人： Dieter Stoll , Thomas Joos , Markus Templin , Oliver Poetz
IPC分类号： C40B30/04 , G01N33/53 , G01N27/26
CPC分类号： G01N33/6842 , C07K16/44
摘要： The present invention relates to a method for the detection and/or enrichment of a large number of different analyte proteins and/or analyte peptides from a sample mixture which includes proteins and/or peptides. The method includes the following steps: a) provision of the sample mixture and, where appropriate, fragmentation of the proteins contained therein into defined peptides, b) provision of first binding molecules which are specific for a peptide epitope of at least one of the various analyte proteins and/or analyte peptides, whereby the peptide epitope includes up to a maximum of five, preferably two to three, amino acids, c) incubation of the first binding molecules with the sample mixture, and d) detection and/or enrichment of the analyte proteins and/or analyte peptides bound to the first binding molecules. The invention also relates to binding molecules which are specific for the terminal peptide epitope of various peptide analytes, whereby the terminal peptide epitope includes the free NH2 group or the free COOH group, one or more than one amino acid defined by the protease specificity, and in each case up to a maximum of three further terminal amino acids.
摘要翻译：本发明涉及从包括蛋白质和/或肽的样品混合物中检测和/或富集大量不同分析物蛋白质和/或分析物肽的方法。该方法包括以下步骤：a）提供样品混合物，并在适当情况下将其中包含的蛋白质碎裂成定义的肽，b）提供对多种抗原决定簇中的至少一种的肽表位是特异性的第一结合分子分析物蛋白质和/或分析物肽，其中肽表位包含最多5个，优选2至3个氨基酸，c）将第一结合分子与样品混合物孵育，以及d）检测和/或富集分析物蛋白和/或与第一结合分子结合的分析物肽。本发明还涉及对各种肽分析物的末端肽表位特异的结合分子，其中末端肽表位包括游离NH 2基团或游离COOH基团，由蛋白酶特异性定义的一个或多于一个氨基酸，以及在每种情况下，最多三个其他末端氨基酸。

3. 发明申请

US20140248715A1 METHOD FOR DETECTING BIOMOLECULES 审中-公开
标题翻译：检测生物分子的方法
公开(公告)号：US20140248715A1
公开(公告)日：2014-09-04
申请号：US14235598
申请日：2012-06-27
申请人： Markus Templin , Fridolin Treindl , Anette Doettinger , Oliver Poetz
发明人： Markus Templin , Fridolin Treindl , Anette Doettinger , Oliver Poetz
IPC分类号： G01N33/543
CPC分类号： G01N33/54306 , G01N33/54313 , G01N33/6842
摘要： A method of detecting biomolecules present in a complex biological sample includes a) separating the biological sample into fractions according to at least one physical property of the biomolecules; and b) specifically detecting biomolecules present in the fractions using at least one solid-phase-based, immunological detection method including immobilizing the biomolecules from the individual fractions on microsphere populations specific for each fraction and distinguishable from one another.
摘要翻译：检测存在于复合生物样品中的生物分子的方法包括：a）根据生物分子的至少一种物理性质将生物样品分离成级分; 和b）使用至少一种基于固相的免疫学检测方法来特异性地检测部分中存在的生物分子，包括将来自各个级分的生物分子固定在对于每个级分特异并且彼此区分的微球群上。

4. 发明授权

US10274485B2 Method for detecting biomolecules 有权
公开(公告)号：US10274485B2
公开(公告)日：2019-04-30
申请号：US14235598
申请日：2012-06-27
申请人： Markus Templin , Fridolin Treindl , Anette Doettinger , Oliver Poetz
发明人： Markus Templin , Fridolin Treindl , Anette Doettinger , Oliver Poetz
IPC分类号： G01N31/00 , G01N33/53 , G01N33/543 , G01N33/68
摘要： A method of detecting biomolecules present in a complex biological sample includes a) separating the biological sample into fractions according to at least one physical property of the biomolecules; and b) specifically detecting biomolecules present in the fractions using at least one solid-phase-based, immunological detection method including immobilizing the biomolecules from the individual fractions on microsphere populations specific for each fraction and distinguishable from one another.

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