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1. 发明授权

US4871663A Exporession vector for human TnF 失效
标题翻译：人类TnF的展示载体
公开(公告)号：US4871663A
公开(公告)日：1989-10-03
申请号：US907816
申请日：1986-09-16
申请人： Takehiro Oshima , Shoji Tanaka , Shigekazu Matsukura
发明人： Takehiro Oshima , Shoji Tanaka , Shigekazu Matsukura
IPC分类号： C12N15/09 , A61K35/12 , A61K35/74 , A61K38/00 , A61K38/16 , C07H21/04 , C07K1/20 , C07K1/22 , C07K14/52 , C07K14/525 , C07K17/10 , C12N15/00 , C12N15/28 , C12N15/70 , C12P21/00 , C12P21/02 , C12R1/19
CPC分类号： C07K14/525 , C12N15/70 , A61K38/00 , Y10S930/144 , Y10S930/31
摘要： The present invention provides a vector plasmid capable of efficient tumor necrosis factor (TNF) production, a process capable of efficient TNF production in a host transformed with said plasmid and a composition containing the TNF produced by said process.The novel plasmid of the present invention is characterized by having inserted therein a DNA fragment that has a phage-derived promoter region upstream of a structural gene for TNF and in which a DNA fragment containing an E. coli gene-derived transcription termination coding base sequence (terminator) is joined immediately downstream of a base sequence coding for the termination of translation of said structural gene.
摘要翻译：本发明提供能够有效的肿瘤坏死因子（TNF）产生的载体质粒，能够在用所述质粒转化的宿主中有效地产生TNF的方法和含有由所述方法产生的TNF的组合物。本发明的新型质粒的特征在于，在其中插入有在TNF结构基因上游具有噬菌体衍生的启动子区域的DNA片段，其中含有大肠杆菌基因的转录终止编码碱基序列的DNA片段（终止子）紧接在编码所述结构基因翻译终止的碱基序列的下游。

2. 发明授权

US5059530A Expression vector for human TNF 失效
标题翻译：人TNF的表达载体
公开(公告)号：US5059530A
公开(公告)日：1991-10-22
申请号：US402675
申请日：1989-09-05
申请人： Takehiro Oshima , Shoji Tanaka , Shigekazu Matsukura
发明人： Takehiro Oshima , Shoji Tanaka , Shigekazu Matsukura
IPC分类号： A61K38/00 , C07K14/525 , C12N1/21 , C12N15/70
CPC分类号： C07K14/525 , C12N15/70 , A61K38/00
摘要： The present invention provides a vector plasmid capable of efficient tumor necrosis factor (TNF) production, a process capable of efficient TNF production in a host transformed with said plasmid and a composition containing the TNF produced by said process.The novel plasmid of the present invention is characterized by having inserted therein a DNA fragment that has a phage-derived promoter region upstream of a structural gene for TNF and in which a DNA fragment containing an E. coli gene-derived transcription termination coding base sequence (terminator) is joined immediately downstream of a base sequence coding for the termination of translation of said structural gene.
摘要翻译：本发明提供能够有效的肿瘤坏死因子（TNF）产生的载体质粒，能够在用所述质粒转化的宿主中有效地产生TNF的方法和含有由所述方法产生的TNF的组合物。本发明的新型质粒的特征在于，在其中插入有在TNF结构基因上游具有噬菌体衍生的启动子区域的DNA片段，其中含有大肠杆菌基因的转录终止编码碱基序列的DNA片段（终止子）紧接在编码所述结构基因翻译终止的碱基序列的下游。

3. 发明授权

US5670340A Process for producing peptides in E. coli 失效
标题翻译：在大肠杆菌中生产肽的方法
公开(公告)号：US5670340A
公开(公告)日：1997-09-23
申请号：US352179
申请日：1994-12-05
申请人： Masayuki Yabuta , Yuji Suzuki , Kazuhiro Ohsuye , Takehiro Oshima , Seiko Onai , Koji Magota , Shoji Tanaka
发明人： Masayuki Yabuta , Yuji Suzuki , Kazuhiro Ohsuye , Takehiro Oshima , Seiko Onai , Koji Magota , Shoji Tanaka
IPC分类号： C12N15/09 , C07K14/58 , C07K14/585 , C12N9/38 , C12N15/16 , C12N15/62 , C12N15/67 , C12N15/70 , C12P21/02 , C12P21/06 , C12R1/19 , C12P21/00
CPC分类号： C07K14/58 , C07K14/585 , C12N15/62 , C12N9/2471 , C12Y302/01023 , C07K2319/00 , C07K2319/23 , C07K2319/43 , C07K2319/50 , C07K2319/75 , C07K2319/90
摘要： The present invention is a process to express a target peptide in a large amount and accumulate the target peptide in host cells in the form of inclusion bodies. The process comprises: A) culturing host cells transformed with a plasmid able to express a gene coding for a fusion protein represented in the formula A--L--B, wherein B is a target peptide, A is a protective peptide comprising a 90-210 amino acid fragment E. coli .beta.-galactosidase, and L is a linker peptide positioned between the C-terminus of the protective peptide and the N-terminus of the target peptide and selected so that when the fusion protein is treated by an enzyme or chemical substance, the target peptide is separated, and wherein the protective peptide and linker peptide are selected so that the isoelectric point of the fusion protein in between 4.9 and 6.9; B) obtaining an insoluble fraction comprising inclusion bodies by homogenization of th cultured transformed cells; C) solubilizing the fusion protein in the inclusion bodies by treatment of the insoluble fraction with a solubilizing agent; and, D) cleaving the peptide bond between the C-terminus of the linker peptide and the N-terminus of the target peptide of the solubilized fusion protein to release the target peptide from the other peptides followed by purification of the target peptide.
摘要翻译：本发明是大量表达目标肽并以包涵体的形式在宿主细胞中聚集靶肽的方法。该方法包括：A）培养用能够表达编码式ALB中表示的融合蛋白的基因的质粒转化的宿主细胞，其中B是靶肽，A是包含90-210个氨基酸片段E的保护肽大肠杆菌β-半乳糖苷酶，L是位于保护肽的C-末端和靶肽的N末端之间的连接肽，并且被选择使得当融合蛋白被酶或化学物质处理时，靶分离肽，并且其中选择保护性肽和接头肽，使得融合蛋白的等电点在4.9和6.9之间; B）通过均质培养的转化细胞获得包含包含体的不溶性级分; C）通过用增溶剂处理不溶性级分将融合蛋白溶解在包涵体中; 并且D）切割接头肽的C末端和溶解的融合蛋白的靶肽的N末端之间的肽键，从其他肽释放靶肽，然后纯化目标肽。

4. 发明授权

US5118615A Peptide and gene coding for same 失效
标题翻译：肽和基因编码相同
公开(公告)号：US5118615A
公开(公告)日：1992-06-02
申请号：US023818
申请日：1987-03-09
申请人： Hisayuki Matsuo , Kenji Kangawa , Yujiro Hayashi , Shinzo Oikawa , Takehiro Oshima , Shoji Tanaka , Hiroshi Nakazato , Yasunori Tawaragi
发明人： Hisayuki Matsuo , Kenji Kangawa , Yujiro Hayashi , Shinzo Oikawa , Takehiro Oshima , Shoji Tanaka , Hiroshi Nakazato , Yasunori Tawaragi
IPC分类号： A61K38/00 , C07K14/58 , C12N15/12 , C12N15/70
CPC分类号： C07K14/58 , C12N15/70 , A61K38/00 , Y10S514/869
摘要： Disclosed is a DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide, plasmids containing the DNA fragment, microorganisms transformed with the plasmid, and a process for production of the peptide using the transformant.Also disclosed is a new peptide consisting of 126 amino acids occurring in human atrium cordis and a precursor thereof. The peptide has notable diuretic action and hypotensive or antihypertensive actions.
摘要翻译：公开了一种DNA片段，其包含编码人心房中出现的具有利尿作用或肽前体的肽的碱基序列，含有该DNA片段的质粒，用质粒转化的微生物，以及使用转化子。还公开了由人心房中出现的126个氨基酸及其前体组成的新肽。肽具有显着的利尿作用和降血压或降压作用。

5. 发明授权

US4987070A Use of a 97 amino acid leader sequence from the E. coli B-galactosidase gene for the production of hanp and hptc as fusion proteins 失效
标题翻译：使用大肠杆菌B-半乳糖苷酶基因的97个氨基酸的前导序列产生作为融合蛋白的hpp和hptc
公开(公告)号：US4987070A
公开(公告)日：1991-01-22
申请号：US163548
申请日：1988-03-03
申请人： Koji Magota , Takehiro Oshima , Shoji Tanaka
发明人： Koji Magota , Takehiro Oshima , Shoji Tanaka
IPC分类号： C12N15/09 , C07K14/58 , C07K14/585 , C12N9/38 , C12N15/62 , C12N15/71 , C12P21/02 , C12R1/19
CPC分类号： C07K14/58 , C07K14/585 , C12N15/62 , C12N15/71 , C12N9/2471 , C12Y302/01023 , C07K2319/00 , C07K2319/02 , C07K2319/61 , C07K2319/75
摘要： A process for the production of a physiologically active peptide (a target peptide) containing cysteine residue, comprising the steps of:(1) culturing Escherichia coli transformed with a plasmid capable of expressing a fusion protein under control of a promoter of E. coli origin or a promoter of a phage origin, wherein the fusion protein is represented by the following formula:A--L--B wherein B represents a target peptide containing cysteine residue; A represents a partner polypeptide consisting of 90 to 220 amino acid residue but not containing cysteine residue; and L represents a linker amino acid residue positioned between C-terminal of the partner polypeptide and N-terminal of the target peptide wherein the same amino acid as the linker amino acid is not present in the target peptide, and the linker amino acid is selected so that the peptide bond between the C-terminal of the linker amino acid and the N-terminal of the target peptide is claimed by a protease or the linker amino acid is selectively degraded by a chemical substance;(2) disrupting the cultured cells and obtaining an insoluble fraction containing the fusion protein;(3) solubilizing the fusion protein with a solubilizing agent, and treating the solubilizing fusion protein with the protease or the chemical substance to liberate the target peptide, and isolating the target peptide.

6. 发明授权

US09416434B2 High-toughness coating film and sliding member 有权
标题翻译：高韧性涂膜和滑动件
公开(公告)号：US09416434B2
公开(公告)日：2016-08-16
申请号：US14385183
申请日：2012-03-16
申请人： Kiyoyuki Kawai , Shoji Tanaka
发明人： Kiyoyuki Kawai , Shoji Tanaka
IPC分类号： C23C14/06 , C22C27/06 , F16J9/26 , C23C14/32
CPC分类号： C22C27/06 , C23C14/0641 , C23C14/32 , F16J9/26 , Y10T428/31678
摘要： Provided is a high-toughness coating film having excellent wear resistance, in particular, resistance to cracking and peeling. A high-toughness coating film (2) includes a composition containing a mixture of metallic chromium, in which nitrogen is dissolved, and Cr2N. A content of the nitrogen in the high-toughness coating film is 2.0 to 8.5 mass %, and IP, which is expressed by total of each diffraction peak intensity of Cr2N/(total of each diffraction peak intensity of metallic chromium+total of each diffraction peak intensity of Cr2N), is 3 to 40% when 2θ is in a range of 30° to 90°, where θ is an incident angle in X-ray diffraction of the high-toughness coating film.
摘要翻译：提供具有优异的耐磨性，特别是耐开裂和剥离的高韧性涂膜。高韧性涂膜（2）包括含有溶解有氮的金属铬与Cr 2 N的混合物的组合物。高韧性涂膜中的氮含量为2.0〜8.5质量％，IP由Cr 2 N的衍射峰强度的总和（各衍射峰强度的金属铬的总和+各衍射的总和）表示当2θ在30°至90°的范围内时，Cr为高于Cr 2 N的峰强度）为3〜40％，其中θ为高韧性涂膜的X射线衍射中的入射角。

7. 发明申请

US20120271830A1 DATA PROCESSING DEVICE 审中-公开
标题翻译：数据处理设备
公开(公告)号：US20120271830A1
公开(公告)日：2012-10-25
申请号：US13516438
申请日：2009-12-16
申请人： Tomohiro Shiino , Yoko Sano , Tsuyoshi Sempuku , Hideto Miyazaki , Kuniyo Ieda , Takashi Sadahiro , Shoji Tanaka
发明人： Tomohiro Shiino , Yoko Sano , Tsuyoshi Sempuku , Hideto Miyazaki , Kuniyo Ieda , Takashi Sadahiro , Shoji Tanaka
IPC分类号： G06F17/30
CPC分类号： G06F16/64 , G06F16/68
摘要： Disclosed is a data processing device including a data acquisition unit for acquiring data from a medium, an integrated database for integrating the data acquired by the data acquisition unit thereinto, a data analysis determination unit for analyzing the data integrated into the integrated database, a display control unit for creating an image of a side view of and an image of a bottom view of a solid expressing the data integrated into the integrated database on the basis of an analysis result acquired by the data analysis determination unit and the data, and a display unit for displaying the images created by the display control unit.
摘要翻译：公开了一种数据处理装置，包括：用于从介质获取数据的数据获取单元，用于将由数据获取单元获取的数据集成到其中的综合数据库，用于分析集成到集成数据库中的数据的数据分析确定单元，控制单元，用于基于由数据分析确定单元获取的分析结果和数据来创建表示集成到集成数据库中的数据的实体的侧视图和底视图的图像;以及显示器用于显示由显示控制单元创建的图像的单元。

8. 发明申请

US20120109516A1 NAVIGATION APPARATUS 有权
标题翻译：导航装置
公开(公告)号：US20120109516A1
公开(公告)日：2012-05-03
申请号：US13266035
申请日：2009-12-08
申请人： Hideto Miyazaki , Tsuyoshi Sempuku , Shoji Tanaka , Kuniyo Ieda , Takashi Sadahiro
发明人： Hideto Miyazaki , Tsuyoshi Sempuku , Shoji Tanaka , Kuniyo Ieda , Takashi Sadahiro
IPC分类号： G01C21/36
CPC分类号： G09B29/102 , G01C21/367
摘要： A navigation apparatus is provided that is highly convenient for a user to view a map screen and a menu window. A display controller 15 determines a view mode of each of a map screen and a menu window, based on information about a travel condition of a moving object from a route search unit 12 and an own-vehicle location identification unit, when the menu window is displayed according to information entered from an input unit 10. A display map generator 16 generates a map screen according to information about the view mode from the display controller 15. A display menu generator 17 generates a menu window according to information about the view mode from the display controller 15. A display unit 21 displays the map screen from the display map generator 16 and the menu window from the menu generator 17.
摘要翻译：提供了一种导航装置，其非常方便用户查看地图屏幕和菜单窗口。显示控制器15基于关于来自路线搜索单元12和本车位置识别单元的移动物体的行驶状况的信息，在菜单窗口为“否”的情况下，确定地图画面和菜单窗口中的每一个的视图模式根据从输入单元10输入的信息显示。显示图生成器16根据来自显示控制器15的关于视图模式的信息生成地图画面。显示菜单生成器17根据关于视图模式的信息生成菜单窗口显示控制器15.显示单元21从显示图生成器16显示地图画面和菜单生成器17的菜单窗口。

9. 发明申请

US20110320117A1 NAVIGATION SYSTEM 审中-公开
标题翻译：导航系统
公开(公告)号：US20110320117A1
公开(公告)日：2011-12-29
申请号：US13202342
申请日：2010-03-26
申请人： Tsuyoshi Sempuku , Shoji Tanaka , Masato Hirai , Hideto Miyazaki , Kuniyo Ieda , Takashi Sadahiro
发明人： Tsuyoshi Sempuku , Shoji Tanaka , Masato Hirai , Hideto Miyazaki , Kuniyo Ieda , Takashi Sadahiro
IPC分类号： G01C21/00
CPC分类号： G01C21/367
摘要： Including a vehicle position determining unit (4, 6, 7, 11) for determining current position of a mobile unit; a map DB 15 that stores map data; a display unit 18 for displaying on its screen the map data and a vehicle mark indicating the position of the mobile unit on a map; an operation input unit 10 for inputting a user operation; a route searching unit 12 for searching for a route to a destination or a route to the destination via a spot on the route; and a map generating unit 17. When the map on the screen moves in response to operation information from the operation input unit 10, the map generating unit reads the map data from the map DB 15, generates a general map including the current position and vehicle mark, and displays it on the display unit 18 as a partial area in the screen.
摘要翻译：包括用于确定移动单元的当前位置的车辆位置确定单元（4,6,7,11）; 存储地图数据的地图DB 15; 显示单元18，用于在其屏幕上显示地图数据和指示移动单元在地图上的位置的车辆标记; 用于输入用户操作的操作输入单元10; 路线搜索单元12，用于经由路线上的一个点搜索到达目的地的路线或到目的地的路线; 地图生成单元17.当屏幕上的地图响应于来自操作输入单元10的操作信息而移动时，地图生成单元从地图DB 15读取地图数据，生成包括当前位置和车辆的一般地图标记，并将其显示在显示单元18上作为屏幕中的局部区域。

10. 发明申请

US20100220104A1 MAP DRAWING DEVICE 审中-公开
标题翻译：地图绘图装置
公开(公告)号：US20100220104A1
公开(公告)日：2010-09-02
申请号：US12676842
申请日：2007-09-13
申请人： Shoji Tanaka
发明人： Shoji Tanaka
IPC分类号： G06T1/00
CPC分类号： G09B29/106 , G06T11/60 , G09B29/003
摘要： A map drawing device includes a drawing parameter DB 7 for storing drawing parameter data which define specifications of map drawing, a drawing parameter managing unit 1 for managing the descriptions of the drawing parameter data, a map drawing unit 9 for carrying out map drawing according to the descriptions of the drawing parameter data managed by the drawing parameter managing unit 1, and a map drawing control unit 8 for controlling the map drawing carried out by the map drawing unit 9 according to the descriptions of the drawing parameter data managed by the drawing parameter managing unit 1.
摘要翻译：地图绘制装置包括用于存储定义地图绘制规格的绘制参数数据的绘图参数DB7，用于管理绘制参数数据的描述的绘图参数管理单元1，根据由绘图参数管理单元1管理的绘制参数数据的描述以及地图绘制控制单元8，用于根据由绘图参数管理的绘制参数数据的描述来控制由地图绘制单元9执行的地图绘制管理单位1。

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