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1. 发明授权

US07741441B2 Method for producing type IV collagen 有权
标题翻译： IV型胶原蛋白的制备方法
公开(公告)号：US07741441B2
公开(公告)日：2010-06-22
申请号：US11727573
申请日：2007-03-27
申请人： Takayuki Kobayashi , Tasuku Sasaki , Hayato Miyoshi
发明人： Takayuki Kobayashi , Tasuku Sasaki , Hayato Miyoshi
IPC分类号： C07K14/00
CPC分类号： C07K14/78
摘要： An object of the invention is to provide type IV collagen without contamination by other proteins and without degradation or denaturation. The present invention provides a type IV collagen which is derived and extracted from lens capsules without the use of an enzyme and has a minimum molecular weight of 160 to 180 kDa measured by SDS-PAGE under reduced conditions.
摘要翻译：本发明的目的是提供IV型胶原蛋白，而不会被其他蛋白质污染，而不会降解或变性。本发明提供IV型胶原蛋白，其在不使用酶的情况下从透镜胶囊衍生和提取，并且在减少的条件下通过SDS-PAGE测量的最小分子量为160至180kDa。

2. 发明申请

US20070232787A1 Method for producing type IV collagen 有权
标题翻译： IV型胶原蛋白的制备方法
公开(公告)号：US20070232787A1
公开(公告)日：2007-10-04
申请号：US11727573
申请日：2007-03-27
申请人： Takayuki Kobayashi , Tasuku Sasaki , Hayato Miyoshi
发明人： Takayuki Kobayashi , Tasuku Sasaki , Hayato Miyoshi
IPC分类号： C07K14/78
CPC分类号： C07K14/78
摘要： An object of the invention is to provide type IV collagen without contamination by other proteins and without degradation or denaturation. The present invention provides a type IV collagen which is derived and extracted from lens capsules without the use of an enzyme and has a minimum molecular weight of 160 to 180 kDa measured by SDS-PAGE under reduced conditions.
摘要翻译：本发明的目的是提供IV型胶原蛋白，而不会被其他蛋白质污染，而不会降解或变性。本发明提供IV型胶原蛋白，其在不使用酶的情况下从透镜胶囊衍生和提取，并且在减少的条件下通过SDS-PAGE测量的最小分子量为160至180kDa。

3. 发明申请

US20080113356A1 Method of Separating and Purifying Nucleic Acid 审中-公开
标题翻译：分离和纯化核酸的方法
公开(公告)号：US20080113356A1
公开(公告)日：2008-05-15
申请号：US11794426
申请日：2006-02-02
申请人： Tasuku Sasaki , Hiroko Inomata , Rie Iwata
发明人： Tasuku Sasaki , Hiroko Inomata , Rie Iwata
IPC分类号： C12Q1/68 , C12N1/08 , C07H21/04
CPC分类号： C12N15/1006
摘要： A method for separating and purifying a nucleic acid comprising steps of: (1) adding a lysis solution to a biomaterial to prepare a sample solution containing a nucleic acid, and adding a water-soluble organic solvent or a solution containing a water-soluble organic solvent to the sample solution thereby preparing a sample solution containing the water-soluble organic solvent; (2) contacting the sample solution containing the water-soluble organic solvent with a solid phase thereby adsorbing the nucleic acid on the solid phase; (3) contacting a washing solution with the solid phase thereby washing the solid phase in a state where the nucleic acid is adsorbed on the solid phase; and (4) contacting a recovering solution with the solid phase thereby desorbing the nucleic acid from the solid phase, wherein, in the step (1), the water-soluble organic solvent or the solution containing the water-soluble organic solvent is added separately in at least two batches.
摘要翻译：一种分离和纯化核酸的方法，包括以下步骤：（1）向生物材料中加入裂解溶液以制备含有核酸的样品溶液，并加入水溶性有机溶剂或含有水溶性有机溶剂溶剂，从而制备含有水溶性有机溶剂的样品溶液; （2）使含有水溶性有机溶剂的样品溶液与固相接触，从而将核酸吸附在固相上; （3）使洗涤液与固相接触，从而在核酸被吸附在固相上的状态下洗涤固相; 和（4）使回收溶液与固相接触，从而从固相中解吸核酸，其中在步骤（1）中，分别加入水溶性有机溶剂或含有水溶性有机溶剂的溶液至少两批。

4. 发明授权

US08961929B2 Targeting agent for tumor site 有权
标题翻译：肿瘤部位靶向剂
公开(公告)号：US08961929B2
公开(公告)日：2015-02-24
申请号：US13520827
申请日：2011-01-07
申请人： Kazutaka Ogiwara , Makoto Ohno , Masayoshi Kojima , Masayuki Kawakami , Chihaya Kakinuma , Tasuku Sasaki , Kiyohito Takada
发明人： Kazutaka Ogiwara , Makoto Ohno , Masayoshi Kojima , Masayuki Kawakami , Chihaya Kakinuma , Tasuku Sasaki , Kiyohito Takada
IPC分类号： A61K51/08 , A61K49/00
CPC分类号： A61K49/0034 , A61K49/0063 , A61K49/0084
摘要： It is an object of the present invention to provide a targeting agent, which enables the delivery of a drug to tumor sites or the imaging of tumor sites by utilizing its effect of accumulating to such tumor sites. The present invention provides a targeting agent for tumor site, which comprises a hydrophilic polymer-modified carrier coated with a gelatin-like protein that has repeats of a sequence represented by Gly-X-Y characteristic to collagen and has two or more sequences of cell adhesion signals in a single molecule wherein X and Y each independently represent an amino acid and a plurality of Gly-X-Y sequences may be the same or different.
摘要翻译：本发明的目的是提供一种靶向剂，其能够通过利用其累积到这种肿瘤部位的作用，将药物递送到肿瘤部位或肿瘤部位的成像。本发明提供了一种用于肿瘤部位的靶向剂，其包含涂覆有明胶样蛋白质的亲水性聚合物改性载体，其具有由Gly-XY表示的对胶原蛋白特征的序列的重复，并且具有两个或更多个细胞粘附信号序列在单分子中，其中X和Y各自独立地表示氨基酸，并且多个Gly-XY序列可以相同或不同。

5. 发明授权

US08920778B2 Targeting agent for tumor site 有权
公开(公告)号：US08920778B2
公开(公告)日：2014-12-30
申请号：US13520827
申请日：2011-01-07
申请人： Kazutaka Ogiwara , Makoto Ohno , Masayoshi Kojima , Masayuki Kawakami , Chihaya Kakinuma , Tasuku Sasaki , Kiyohito Takada
发明人： Kazutaka Ogiwara , Makoto Ohno , Masayoshi Kojima , Masayuki Kawakami , Chihaya Kakinuma , Tasuku Sasaki , Kiyohito Takada
IPC分类号： A61K51/08 , A61K49/00
摘要： It is an object of the present invention to provide a targeting agent, which enables the delivery of a drug to tumor sites or the imaging of tumor sites by utilizing its effect of accumulating to such tumor sites. The present invention provides a targeting agent for tumor site, which comprises a hydrophilic polymer-modified carrier coated with a gelatin-like protein that has repeats of a sequence represented by Gly-X-Y characteristic to collagen and has two or more sequences of cell adhesion signals in a single molecule wherein X and Y each independently represent an amino acid and a plurality of Gly-X-Y sequences may be the same or different.

6. 发明申请

US20130017147A1 TARGETING AGENT FOR TUMOR SITE 有权
标题翻译： TUMOR SITE的目标代理
公开(公告)号：US20130017147A1
公开(公告)日：2013-01-17
申请号：US13520827
申请日：2011-01-07
申请人： Kazutaka Ogiwara , Makoto Ohno , Masayoshi Kojima , Masayuki Kawakami , Chihaya Kakinuma , Tasuku Sasaki , Kiyohito Takada
发明人： Kazutaka Ogiwara , Makoto Ohno , Masayoshi Kojima , Masayuki Kawakami , Chihaya Kakinuma , Tasuku Sasaki , Kiyohito Takada
IPC分类号： A61K49/00 , A61P35/00 , A61K51/12 , A61K49/18 , A61K9/00 , A61K9/127
CPC分类号： A61K49/0034 , A61K49/0063 , A61K49/0084
摘要： It is an object of the present invention to provide a targeting agent, which enables the delivery of a drug to tumor sites or the imaging of tumor sites by utilizing its effect of accumulating to such tumor sites. The present invention provides a targeting agent for tumor site, which comprises a hydrophilic polymer-modified carrier coated with a gelatin-like protein that has repeats of a sequence represented by Gly-X—Y characteristic to collagen and has two or more sequences of cell adhesion signals in a single molecule wherein X and Y each independently represent an amino acid and a plurality of Gly-X—Y sequences may be the same or different.
摘要翻译：本发明的目的是提供一种靶向剂，其能够通过利用其累积到这种肿瘤部位的作用，将药物递送到肿瘤部位或肿瘤部位成像。本发明提供了一种用于肿瘤部位的靶向剂，其包含涂覆有明胶样蛋白质的亲水性聚合物改性载体，其具有由胶原特征的Gly-X-Y表示的序列的重复并具有两个或更多个细胞序列单分子中的粘合信号，其中X和Y各自独立地表示氨基酸，并且多个Gly-X-Y序列可以相同或不同。

7. 发明申请

US20100063268A1 NUCLEIC ACID EXTRACTION METHOD 审中-公开
标题翻译：核酸提取方法
公开(公告)号：US20100063268A1
公开(公告)日：2010-03-11
申请号：US11993352
申请日：2006-09-28
申请人： Hideyuki Kanehara , Tasuku Sasaki
发明人： Hideyuki Kanehara , Tasuku Sasaki
IPC分类号： C07H21/00
CPC分类号： C12N15/1006
摘要： A method for extracting a nucleic acid, which comprises: (a) preparing a biomaterial containing a solution by a following step (i) or (ii): (i) a step in which a biomaterial containing a phosphate buffer solution or a Bis-Tris (N,N-bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane) buffer solution is prepared; or (ii) a step in which a buffer solution contained in a biomaterial is replaced with a Bis-Tris buffer solution; (b) dissolving the biomaterial with a lysis solution, and eluting a nucleic acid contained in the biomaterial; (c) preparing a lysate solution by adding a water-soluble organic solvent to the nucleic acid-eluted solution obtained in the step (b); (d) allowing the nucleic acid contained in the lysate solution to be adsorbed by a solid material; (e) washing impurities remaining in the solid material and the lysis solution; and (f) desorbing the absorbed nucleic acid from the solid material by a recovering solution.
摘要翻译：一种提取核酸的方法，其包括：（a）通过以下步骤（i）或（ii）制备含有溶液的生物材料：（i）其中含有磷酸盐缓冲溶液或Bis- 制备三（N，N-双（2-羟乙基）亚氨基三（羟甲基）甲烷）缓冲溶液; 或者（ii）将生物材料中所含的缓冲溶液置换为Bis-Tris缓冲溶液的步骤; （b）用裂解溶液溶解生物材料，并洗脱生物材料中所含的核酸; （c）通过向步骤（b）中获得的核酸洗脱溶液中加入水溶性有机溶剂来制备裂解物溶液; （d）允许溶解产物溶液中所含的核酸被固体材料吸附; （e）洗涤残留在固体材料和裂解溶液中的杂质; 和（f）通过回收溶液从固体材料中解吸所吸收的核酸。

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