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1. 发明申请

US20090098600A1 THERMOTOLERANT RIBONUCLEASE H 有权
标题翻译：热稳定剂RIBONUCLEASE H
公开(公告)号：US20090098600A1
公开(公告)日：2009-04-16
申请号：US12194364
申请日：2008-08-19
申请人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
IPC分类号： C12P21/04 , C12N9/22 , C12N15/11 , C12N15/00 , C12N1/21
CPC分类号： C12N9/22
摘要： Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译：具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。

2. 发明授权

US08008055B2 Thermotolerant ribonuclease H 有权
标题翻译：耐热核糖核酸酶H
公开(公告)号：US08008055B2
公开(公告)日：2011-08-30
申请号：US12194364
申请日：2008-08-19
申请人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
IPC分类号： C12N9/22 , C12N9/14
CPC分类号： C12N9/22
摘要： Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译：具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。

3. 发明授权

US07422888B2 Thermotolerant ribonuclease H 有权
标题翻译：耐热核糖核酸酶H
公开(公告)号：US07422888B2
公开(公告)日：2008-09-09
申请号：US10380430
申请日：2001-09-13
申请人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12N9/22 , C12N9/14 , C07H21/04
CPC分类号： C12N9/22
摘要： Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译：具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。

4. 发明授权

US08344105B2 Polypeptides having DNA polymerase activity 有权
标题翻译：具有DNA聚合酶活性的多肽
公开(公告)号：US08344105B2
公开(公告)日：2013-01-01
申请号：US13239981
申请日：2011-09-22
申请人： Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人： Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12P19/34 , C07K1/00
CPC分类号： C12Q1/686 , C12N9/1252 , C12Q2521/101
摘要： A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
摘要翻译：具有高保真DNA聚合酶活性的多肽，因此可用作遗传工程试剂; 编码该多肽的基因; 一种产生该多肽的方法; 以及通过使用多肽扩增核酸的方法。

5. 发明授权

US08367328B2 Method for synthesizing DNA 有权
标题翻译： DNA合成方法
公开(公告)号：US08367328B2
公开(公告)日：2013-02-05
申请号：US12285866
申请日：2008-10-15
申请人： Kiyozo Asada , Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroyuki Mukai , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12P19/34 , C12N9/1252 , C12N9/96 , C12Q1/6806 , Y10S435/975 , C12Q2521/107
摘要： A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.
摘要翻译：一种DNA合成反应增强剂，其包含选自酸性物质和阳离子配合物中的至少一种; DNA合成方法，其中在DNA合成反应中，通过使用DNA聚合酶在上述增强剂的存在下进行反应; 包含上述增强剂的DNA合成反应组合物; 包含两种以上各自具有3'→5'核酸外切酶活性的DNA聚合酶的DNA合成反应组合物; 一种DNA合成方法，其中在DNA合成反应中使用两种或更多种具有3'→5'核酸外切酶活性的DNA聚合酶; 用于体外DNA合成的试剂盒，其包含两种或更多种各自具有3'→5'核酸外切酶活性的DNA聚合酶; 以及用于体外DNA合成的试剂盒，其中试剂盒包含DNA合成反应增强剂和DNA聚合酶。根据本发明，与常规DNA合成反应相比，可以以更优异的效果进行DNA合成。

6. 发明授权

US07846703B2 Method for enhancing polymerase activity 有权
标题翻译：增强聚合酶活性的方法
公开(公告)号：US07846703B2
公开(公告)日：2010-12-07
申请号：US11866148
申请日：2007-10-02
申请人： Eiji Kobayashi , Yuki Ueda , Yoshimi Sato , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人： Eiji Kobayashi , Yuki Ueda , Yoshimi Sato , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12N9/10 , C12N9/96 , C07K14/00 , C12Q1/66 , C12P19/34
CPC分类号： C12N9/1241
摘要： A polymerase activity is effectively enhanced by adding an anionic surfactant, in particular an anionic surfactant having a polyethoxyl group, to a reaction mixture containing a polymerase.
摘要翻译：通过向含有聚合酶的反应混合物中加入阴离子表面活性剂，特别是具有聚乙氧基的阴离子表面活性剂，可有效提高聚合酶活性。

7. 发明授权

US06218150B1 DNA polymerase-related factors 有权
公开(公告)号：US06218150B1
公开(公告)日：2001-04-17
申请号：US09446504
申请日：1999-12-23
申请人： Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12P1934
CPC分类号： C12N9/1252
摘要： The present invention relates to a thermostable DNA polymerase-associated factor capable of enhancing DNA synthesizing-activity of a DNA polymerase; a thermostable DNA polymerase-associated factor possessing an activity of binding to a DNA polymerase and a method for producing the same; a gene encoding the DNA polymerase-associated factor; a method of DNA synthesis by using a DNA polymerase in the presence of the DNA polymerase-associated factor; and a kit comprising the DNA polymerase-associated factor. According to the present invention, there can be provided in vitro DNA synthesis and a DNA amplification system which are more excellent than conventional techniques by utilizing the DNA polymerase-associated factor of the present invention.

8. 发明授权

US07704713B2 Polypeptides having DNA polymerase activity 有权
标题翻译：具有DNA聚合酶活性的多肽
公开(公告)号：US07704713B2
公开(公告)日：2010-04-27
申请号：US11628268
申请日：2005-05-12
申请人： Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人： Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12P19/34 , C07K14/00
CPC分类号： C12Q1/686 , C12N9/1252 , C12Q2521/101
摘要： A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
摘要翻译：具有高保真DNA聚合酶活性的多肽，因此可用作遗传工程试剂; 编码该多肽的基因; 一种产生该多肽的方法; 以及通过使用多肽扩增核酸的方法。

9. 发明申请

US20090098613A1 Method for synthesizing DNA 有权
公开(公告)号：US20090098613A1
公开(公告)日：2009-04-16
申请号：US12285866
申请日：2008-10-15
申请人： Kiyozo Asada , Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroyuki Mukai , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N9/1252 , C12N9/96 , C12Q1/6806 , Y10S435/975 , C12Q2521/107
摘要： A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.

10. 发明申请

US20090098610A1 Polypeptides having dna polymerase activity 有权
标题翻译：具有dna聚合酶活性的多肽
公开(公告)号：US20090098610A1
公开(公告)日：2009-04-16
申请号：US11628268
申请日：2005-05-12
申请人： Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人： Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12P21/00 , C12N9/10 , C12P19/34 , C07K16/00 , C07H21/04
CPC分类号： C12Q1/686 , C12N9/1252 , C12Q2521/101
摘要： A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
摘要翻译：具有高保真DNA聚合酶活性的多肽，因此可用作遗传工程试剂; 编码该多肽的基因; 一种产生该多肽的方法; 以及通过使用多肽扩增核酸的方法。

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