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    • 3. 发明申请
    • Modified hydrogenase, enzymatic electrode made of modified hydrogenase, and hydrogenase modification method
    • 改性氢化酶,由改性氢化酶制成的酶电极和氢化酶修饰方法
    • US20090081761A1
    • 2009-03-26
    • US12213081
    • 2008-06-13
    • Hidetaka NishikooriHirofumi NishiharaKi-Seok Yoon
    • Hidetaka NishikooriHirofumi NishiharaKi-Seok Yoon
    • C12N9/14
    • H01M8/16C12N9/0067Y02E60/527
    • The invention provides: (1) a modified hydrogenase obtained by removing electron-transfer sites from a hydrogenase constituted of: active subunits including active sites having a hydrogen oxidization-reduction activity; and electron-transfer subunits having the electron-transfer sites through which electrons are transferred between the active sites and the outside of the hydrogenase; (2) a modified hydrogenase obtained from a hydrogenase, wherein when the hydrogenase is isolated from bacteria that produces the hydrogenase, a process for exposing the hydrogenase to an oxygen atmosphere is executed; (3) an enzymatic electrode made of at least one of the foregoing modified hydrogenases; and (4) a hydrogenase modification method including: a step of isolating from hydrogenase-producing bacteria; and a step of removing the electron-transfer sites of the electron-transfer subunits from the hydrogenase by exposing the hydrogenase to an oxygen atmosphere.
    • 本发明提供:(1)通过从由以下构成的氢化酶除去电子转移位点而获得的修饰氢化酶:活性亚基,其包括具有氢氧化还原活性的活性位点; 和具有电子转移位点的电子转移亚单位,电子在活性位点和氢化酶的外部之间转移; (2)从氢化酶获得的修饰氢化酶,其中当氢化酶从产生氢化酶的细菌中分离时,执行将氢化酶暴露于氧气氛的方法; (3)由上述改性氢化酶中的至少一种制成的酶电极; 和(4)氢化酶修饰方法,包括:从产氢细菌中分离的步骤; 以及通过将氢化酶暴露于氧气氛中从氢化酶除去电子转移亚单位的电子转移位点的步骤。