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1. 发明授权

US5571727A Labelling colors for detecting cocaine or methamphetamine, method of preparing the same and detector for cocaine or methamphetamine 失效
标题翻译：用于检测可卡因或甲基苯丙胺的标签颜色，其制备方法和可卡因或甲基苯丙胺检测器
公开(公告)号：US5571727A
公开(公告)日：1996-11-05
申请号：US319976
申请日：1994-10-07
申请人： Nobuyuki Shigetoh , Hiroshi Nakayama , Jinsei Miyazaki , Tadayasu Mitsumata
发明人： Nobuyuki Shigetoh , Hiroshi Nakayama , Jinsei Miyazaki , Tadayasu Mitsumata
IPC分类号： C07D209/14 , C07D471/08 , G01N33/532 , G01N33/542 , G01N33/94 , C07D401/06 , G01N33/533
CPC分类号： C07D471/08 , C07D209/14 , G01N33/532 , G01N33/542 , G01N33/946 , Y10S436/816
摘要： A labelling color for detecting cocaine of Formula (I) ##STR1## wherein X represents a halogen, and a method of detecting methamphetamine, including the steps of adding a labelling color of Formula (II) ##STR2## wherein X represents an anion to a first solution of methamphetamine antibody to form a labelling color-methamphetamine antibody complex, measuring the fluorescence of the first solution, adding a sample containing an unknown amount of methamphetamine to the first solution to form a second solution, measuring the fluorescence of the second solution, and correlating any change in the fluorescence between the first and second solutions to the concentration of methamphetamine in the sample.
摘要翻译：用于检测式（I）的可卡因的标记颜色其中X表示卤素的式（I）和检测甲基苯丙胺的方法，包括以下步骤：添加式（II）的标记颜色），其中X表示甲基苯丙胺抗体的第一溶液的阴离子，以形成标记颜色 - 甲基苯丙胺抗体复合物，测量第一溶液的荧光，向第一溶液中加入含有未知量的甲基苯丙胺的样品以形成第二溶液，测量第二溶液的荧光，并将第一和第二溶液之间的荧光的任何变化与样品中甲基苯丙胺的浓度相关联。

2. 发明授权

US06174723B1 Cocaine derivative, protein conjugate thereof, monoclonal antibody producing cell line, method for preparing the cell line and monoclonal antibody 失效
标题翻译：可卡因衍生物，其蛋白质缀合物，产生单克隆抗体的细胞系，制备细胞系的方法和单克隆抗体
公开(公告)号：US06174723B1
公开(公告)日：2001-01-16
申请号：US08982963
申请日：1997-12-02
申请人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
发明人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
IPC分类号： C12N506
CPC分类号： G01N33/946 , A61K47/60 , A61K47/643 , C07D451/12 , C07K16/16
摘要： A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives. A monoclonal antibody, a monoclonal antibody producing cell line, and a method for producing the monoclonal antibody producing cell line by using the above cocaine-protein conjugate as an immunogen is also described. The method of the invention comprises preparing a cocaine-protein conjugate with a pyridyl dithiococaine derivative of Formula (I): where R is either H or R′; and where R′ is: wherein n=1 to 4 and Ph is a benzene ring, and wherein said monoclonal antibody detects 10−9 M cocaine; and which derivative binds to a protein by a disulfide bond; immunizing A/J mouse with the conjugate; fusing its spleen cell and a myeloma cell line; cloning the fusion to produce a monoclonal antibody producing cell line; culturing the cell line, and purifying a supernatant of the culture to form a monoclonal antibody to specifically bind to cocaine or cocaine derivatives.
摘要翻译：通过使用具有甲氧基羰基和苯甲酰基的可卡因衍生物容易地制备可卡因 - 蛋白质结合物的方法。该缀合物可用于检测可卡因或可卡因衍生物。还描述了单克隆抗体，产生单克隆抗体的细胞系，以及通过使用上述可卡因蛋白质缀合物作为免疫原来制备产生单克隆抗体的细胞系的方法。本发明的方法包括用式（I）的吡啶基二硫代可卡因衍生物制备可卡因蛋白质结合物：其中R是H或R'; 其中R'为：其中n = 1-4，Ph为苯环，其中所述单克隆抗体检测10-9M可卡因; 哪个衍生物通过二硫键与蛋白质结合; 用缀合物免疫A / J小鼠; 融合其脾细胞和骨髓瘤细胞系; 克隆融合物以产生产生单克隆抗体的细胞系; 培养细胞系，并纯化培养物的上清液以形成特异性结合可卡因或可卡因衍生物的单克隆抗体。

3. 发明授权

US06271381B1 Cocaine derivative, protein conjugate thereof, monoclonal antibody producing cell line, method for preparing the cell line and monoclonal antibody 失效
标题翻译：可卡因衍生物，其蛋白质缀合物，产生单克隆抗体的细胞系，制备细胞系的方法和单克隆抗体
公开(公告)号：US06271381B1
公开(公告)日：2001-08-07
申请号：US09706774
申请日：2000-11-07
申请人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
发明人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
IPC分类号： C07D40100
CPC分类号： G01N33/946 , A61K47/60 , A61K47/643 , C07D451/12 , C07K16/16
摘要： A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives.
摘要翻译：通过使用具有甲氧基羰基和苯甲酰基的可卡因衍生物容易地制备可卡因 - 蛋白质结合物的方法。该缀合物可用于检测可卡因或可卡因衍生物。

4. 发明授权

US5378634A Labelling color for detecting methamphetamine 失效
标题翻译：用于检测甲基苯丙胺的标签颜色
公开(公告)号：US5378634A
公开(公告)日：1995-01-03
申请号：US102426
申请日：1993-08-05
申请人： Nobuyuki Sigetoh , Jinsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
发明人： Nobuyuki Sigetoh , Jinsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
IPC分类号： C09B23/08 , G01N33/58 , G01N33/00
CPC分类号： C09B23/083 , G01N33/582 , Y10S435/968 , Y10S436/816 , Y10S436/901 , Y10T436/14
摘要： A labelling color which includes a pentamethine of the following formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 each are a C.sub.1-6 alkyl group, and X is an anion.
摘要翻译：包括下式（1）的五甲氧基的标记颜色：其中R 1，R 2，R 3和R 4各自为C 1-6烷基，X为阴离子。

5. 发明授权

US5243074A Immunoreagents 失效
标题翻译：免责声明
公开(公告)号：US5243074A
公开(公告)日：1993-09-07
申请号：US616655
申请日：1990-11-21
申请人： Tadayasu Mitsumata , Jinsei Miyazaki
发明人： Tadayasu Mitsumata , Jinsei Miyazaki
IPC分类号： G01N33/533 , G01N33/58
CPC分类号： G01N33/533 , G01N33/582 , Y10S436/815 , Y10S436/816 , Y10S436/901
摘要： An immunofluorescent assay method, characterized in using an immunodetective reagent, which is an antigen-like substance comprising a fluorescent substance and an antigen chemically bonded thereto with or without intervention of an additional chemical bond, said fluorescent substance being changed in the wavelength or intensity of the fluorescence when an antibody comes close thereto, and said antigen being specific to said antibody.

6. 发明授权

US5233025A Amphetamine protein complex as immunogen for obtaining antibodies specific to methamphetamine 失效
标题翻译：安非他明蛋白复合物作为获得甲基苯丙胺特异性抗体的免疫原
公开(公告)号：US5233025A
公开(公告)日：1993-08-03
申请号：US651809
申请日：1991-02-07
申请人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
IPC分类号： A61K47/48 , C07K16/44 , G01N33/531
CPC分类号： G01N33/531 , A61K47/4833 , C07K16/44
摘要： A N-(4-aminobutyl)amphetamine-protein complex has been synthesized to be used as an immunogen to prepare an antibody having a higher affinity to methamphetamine that to the hapten N-(4-aminobutyl)amphetamine. The antibody prepared by the use of the complex has 1,000 times the affinity to methamphetamine as that of an antibody prepeared by the use of N-(4-aminobutyl)methamphetamine-protein complex as an immunogen.
摘要翻译：已经合成了N-（4-氨基丁基）苯丙胺 - 蛋白质复合物作为免疫原，以制备对半抗原N-（4-氨基丁基）苯丙胺具有较高的对甲基苯丙胺的亲和力的抗体。通过使用该复合物制备的抗体与通过使用N-（4-氨基丁基）甲基苯丙胺 - 蛋白复合物作为免疫原而展示的抗体的甲基苯丙胺的亲和力为1,000倍。

7. 发明授权

US5229302A Fluorescence immunoassay method utilizing pseudo-antigens combined with fluorescent quenchers 失效
标题翻译：荧光免疫测定法利用假抗原与荧光猝灭剂结合
公开(公告)号：US5229302A
公开(公告)日：1993-07-20
申请号：US830442
申请日：1992-02-03
申请人： Jinsei Miyazaki , Noboru Motoyama , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Noboru Motoyama , Tadayasu Mitsumata
IPC分类号： G01N33/532 , G01N33/542 , G01N33/94
CPC分类号： G01N33/542 , G01N33/532 , G01N33/946 , Y10S436/816
摘要： A fluorescence immunoassay method is described in which the presence or absence of antigen is measured within one minute by measuring a variation in fluorescence emitted from an antibody. The method comprises reaction between a pseudo-antigen obtained by chemical combination of an antigen and a fluorescent quencher and an antibody by which fluorescence to be emitted from the antibody is quenched by the action of the quencher combined. When an analyte is added to a solution of the combination, the pseudo-antigen is replaced by an antigen if the analyte contains the antigen, so that the fluorescence increases in intensity. This intensity is measured and compared with the initially measured intensity to detect the presence of the antigen. The method does not make use of the action of antigen on antibody with respect to the fluorescence of the antibody and is applicable to all antigens.
摘要翻译：描述了荧光免疫测定方法，其中通过测量从抗体发射的荧光的变化，在一分钟内测量抗原的存在或不存在。该方法包括通过抗原和荧光猝灭剂的化学组合获得的假抗原与通过猝灭剂组合的作用淬灭从抗体发射的荧光的抗体之间的反应。当将分析物加入到组合的溶液中时，如果分析物含有抗原，假抗原被抗原替代，使得荧光强度增加。测量该强度并与初始测量的强度进行比较以检测抗原的存在。该方法没有利用抗体对抗体对抗体的荧光的作用，适用于所有抗原。

8. 发明授权

US5026827A Amphetamine-protein complex as immunogen for obtaining antibodies specific to methamphetamine 失效
标题翻译：安非他明 - 蛋白复合物作为获得甲基苯丙胺特异性抗体的免疫原
公开(公告)号：US5026827A
公开(公告)日：1991-06-25
申请号：US401247
申请日：1989-08-31
申请人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
IPC分类号： A61K47/48 , C07K16/44 , G01N33/531
CPC分类号： G01N33/531 , A61K47/4833 , C07K16/44
摘要： A N-(4-aminobutyl)amphetamine-protein complex has been synthesized to be used as an immunogen to prepare an antibody having a high affinity to methamphetamine. The antibody prepared by the use of the complex has 1,000 times the affinity to methamphetamine as that of an antibody prepared by the use of N-(4-aminobutyl)methamphetamine-protein complex as an immunogen.

9. 发明申请

US20080275721A1 Information Terminal and Health Care Assisting System 审中-公开
标题翻译：信息终端和保健辅助系统
公开(公告)号：US20080275721A1
公开(公告)日：2008-11-06
申请号：US11570592
申请日：2005-07-01
申请人： Kazutoshi Nagai , Jinsei Miyazaki
发明人： Kazutoshi Nagai , Jinsei Miyazaki
IPC分类号： G06Q50/00 , G06Q10/00
CPC分类号： G06F19/3475 , G06Q50/22
摘要： The invention is intended for making it possible for the user to get appropriate advice not only based on biological information, but also considering the situation of health care to be taken by the user as for article intake.Food information of food ingredients, food-contained calories, etc., described on a package, etc., of food incepted by the user is input to a patient terminal 4 through food information input means 3 for inputting the food information and the food information is transmitted to a server 1 via a communication line 2.
摘要翻译：本发明旨在使用户不仅可以基于生物信息获得适当的建议，而且还考虑到用户对于摄取物品所采取的卫生保健的情况。食品信息输入装置3通过用于输入食物信息和食品信息的食品信息输入装置3向患者终端4输入食品成分，食品卡路里等食物信息，经由通信线路2发送到服务器1。

10. 发明授权

US06303759B1 Dye-labeled and polymerized antibody and method for preparing the same 失效
标题翻译：染料标记和聚合抗体及其制备方法
公开(公告)号：US06303759B1
公开(公告)日：2001-10-16
申请号：US09310850
申请日：1999-05-13
申请人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
发明人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
IPC分类号： C07K1702
CPC分类号： G01N33/531 , G01N33/532 , Y10S436/80
摘要： The present invention provides a highly sensitive dye-labeled and polymerized antibody that can detect a target substance even when the target substance has a low concentration. The dye-labeled and polymerized antibody of the present invention comprises a polymerized antibody, which has been polymerized with a polyfunctional reagent, and a cyanine dye for labeling the polymerized antibody represented by the formula (1) given below: where R1 and R2 denote hydrogen or an alkyl group, X denotes a halogen, M denotes hydrogen or an alkali metal, and n represents an integer in a range of 1 to 4.
摘要翻译：本发明提供即使在目标物质浓度低的情况下也能够检测目标物质的高灵敏度的染料标记和聚合抗体。本发明的染料标记和聚合抗体包含已经与多官能试剂聚合的聚合抗体和用于标记由下式给出的聚合抗体的花青染料：其中R1和R2表示氢或烷基，X表示卤素，M表示氢或碱金属，n表示1〜4的整数。

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