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1. 发明申请

US20080287319A1 Separation Method and Apparatus of Single-Stranded Nucleic Acid, Microarray and Dna Chip 审中-公开
标题翻译：单链核酸，微阵列和Dna芯片的分离方法和装置
公开(公告)号：US20080287319A1
公开(公告)日：2008-11-20
申请号：US11816575
申请日：2006-02-27
申请人： Tadashi Matsunaga , Haruko Takeyama , Tsuyoshi Tanaka , Takeo Tanaami , Saya Sato , Hisao Katakura , Yuji Mitsumori , Takeyuki Mogi
发明人： Tadashi Matsunaga , Haruko Takeyama , Tsuyoshi Tanaka , Takeo Tanaami , Saya Sato , Hisao Katakura , Yuji Mitsumori , Takeyuki Mogi
IPC分类号： C40B40/08 , C07H1/00
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q1/6837 , C12Q2563/131 , C12Q2523/113
摘要： A separation method of single-stranded nucleic acid characterized in that nucleic acid amplification is performed using a first primer to which a second substance capable of binding specifically to a first substance is bound and a second primer to which the second substance is not bound, and double-stranded nucleic acid obtained by the nucleic acid amplification is bound to the first substance, and the double-stranded nucleic acid bound to the first substance is dissociated into a single strand, and a separation apparatus of single-stranded nucleic acid characterized by having a nucleic acid amplification part 1 for performing nucleic acid amplification using a first primer to which a second substance capable of binding specifically to a first substance is bound and a second primer to which the second substance is not bound, a binding part 2 for binding double-stranded nucleic acid obtained by the nucleic acid amplification to the first substance, and a dissociation part 3 for dissociating the double-stranded nucleic acid bound to the first substance into a single strand.
摘要翻译：单链核酸的分离方法，其特征在于，使用与第一物质结合的能够结合第二物质的第一物质与第二物质不结合的第二引物的第一引物进行核酸扩增，通过核酸扩增获得的双链核酸与第一物质结合，将与第一物质结合的双链核酸解离为单链，单链核酸的分离装置的特征在于，具有用于使用与第一物质结合特异性的第二物质结合的第一引物和第二物质不结合的第二引物进行核酸扩增的核酸扩增部分1，用于结合双重结合部分2 通过对第一物质进行核酸扩增获得的品种核酸，以及用于dissocia的解离部分3 将与第一物质结合的双链核酸结合成单链。

2. 发明申请

US20110057145A1 METHOD FOR PREPARING DENDRIMER-MODIFIED, MAGNETIC FINE PARTICLES 审中-公开
标题翻译：制备改性改性的磁性颗粒的方法
公开(公告)号：US20110057145A1
公开(公告)日：2011-03-10
申请号：US12876562
申请日：2010-09-07
申请人： Tadashi Matsunaga , Tsuyoshi Tanaka , Keiichi Hatakeyama , Takeo Tanaami , Hitoshi Wake , Tomoyuki Taguchi , Takeyuki Mogi
发明人： Tadashi Matsunaga , Tsuyoshi Tanaka , Keiichi Hatakeyama , Takeo Tanaami , Hitoshi Wake , Tomoyuki Taguchi , Takeyuki Mogi
IPC分类号： H01F1/00
CPC分类号： H01F1/42
摘要： There is provided a method for preparing dendrimer-modified magnetic fine particles wherein such particles can be made within a shorter time and more inexpensively than in the above-stated prior art processes and lot-to-lot variations in properties are lessened. The method for preparing dendrimer-fixed magnetic fine particles comprises the steps of providing magnetic particles having a functional group at a surface thereof, providing a dendrimer having a functional group at a base end portion thereof and synthesized to a desired generation and binding the functional group of the magnetic particles and the functional group of the dendrimer directly or indirectly through a crosslinking agent.
摘要翻译：提供了一种制备树枝状聚合物改性的磁性细颗粒的方法，其中这种颗粒可以在比上述现有技术方法更短的时间和更廉价的情况下制备，并且批次间的性质变化减少。制备树枝状大分子固定磁性微粒的方法包括以下步骤：在其表面提供具有官能团的磁性颗粒，提供在其基端部分具有官能团的树枝状聚合物，并合成所需的一代并结合官能团的磁性颗粒和树枝状聚合物的官能团直接或间接地通过交联剂。

3. 发明申请

US20070068964A1 Chemical reaction cartridge and method using same 有权
标题翻译：化学反应筒及其使用方法
公开(公告)号：US20070068964A1
公开(公告)日：2007-03-29
申请号：US11524948
申请日：2006-09-22
申请人： Takeo Tanaami , Hidetoshi Aoki , Saya Satou , Takeyuki Mogi
发明人： Takeo Tanaami , Hidetoshi Aoki , Saya Satou , Takeyuki Mogi
IPC分类号： B65D35/22 , B65D35/28 , B67D5/52 , B67D5/60
CPC分类号： B01L3/502707 , B01F5/0641 , B01F5/0645 , B01F5/0693 , B01F5/0696 , B01F13/0059 , B01F15/0201 , B01F15/025 , B01J19/0093 , B01J2219/00783 , B01J2219/00833 , B01J2219/00835 , B01J2219/0086 , B01J2219/00889 , B01J2219/00891 , B01L3/5027 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481
摘要： A chemical reaction cartridge enabling chemical reaction to be stably and safely carried out therein, and a method of using the same are provided. A chemical reaction cartridge is provided with a substrate, and an elastic member 2 overlaid on the substrate. A recess of a predetermined shape, depressed toward the top surface of the elastic member is formed in the back surface of the elastic member. The recess creates space between the substrate, and the elastic member, thereby making up a well, for holding the main ingredient of the epoxy adhesive, a flow path communicating with the well, a well for holding a curing agent for the epoxy adhesive, a flow path communicating with the well, a mixing part for mixing the main ingredient with the curing agent, and a discharge path for discharging a mixture. While the chemical reaction cartridge is not in use as yet, the flow paths, and the discharge path are closed, so that the wells are, in effect, in a hermetically sealed condition. The mixing part is made up of a plurality of flow paths intersecting each other. The respective starting ends of the flow paths are each linked with the flow path, or the flow path, and the respective terminals of the flow paths are linked with the discharge path. The terminal of the discharge path communicates with the outside via an end face of the cartridge.
摘要翻译：能够使化学反应稳定且安全地进行的化学反应筒及其使用方法。化学反应盒设置有基板和覆盖在基板上的弹性部件2。在弹性部件的背面形成有朝向弹性部件的上表面凹陷的规定形状的凹部。凹部在基板和弹性部件之间形成空间，从而构成一个孔，用于保持环氧粘合剂的主要成分，与该孔连通的流路，用于保持环氧粘合剂的固化剂的孔，与井连通的流路，用于混合主成分与固化剂的混合部以及排出混合物的排出路径。虽然化学反应盒尚未使用，但是流动路径和排放路径是关闭的，因此实际上这些井处于气密密封状态。混合部分由彼此相交的多个流路组成。流路的各个起始端各自与流路或流路连通，流路的各端子与排出路径连结。排出路径的端子经由筒的端面与外部连通。

4. 发明申请

US20090221091A1 CHEMICAL REACTION CARTRIDGE, MIXTURE GENERATING METHOD AND CONTROL DEVICE OF CHEMICAL REACTION CARTRIDGE 审中-公开
标题翻译：化学反应罐，化学反应罐混合发生方法及控制装置
公开(公告)号：US20090221091A1
公开(公告)日：2009-09-03
申请号：US12396148
申请日：2009-03-02
申请人： Takeyuki Mogi , Takeo Tanaami
发明人： Takeyuki Mogi , Takeo Tanaami
IPC分类号： G01N21/00 , B01J19/00 , G01N1/00
CPC分类号： B01F11/0045 , B01F15/0087 , B01F15/0203 , B01F15/0256 , B01L3/50273 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , Y10T436/25
摘要： Disclosed is a chemical reaction cartridge including an elastic body as a construction material and a flow path and two or more chambers connected by the flow path formed inside the cartridge, and the cartridge is structured so as to move or block a fluid substance in the flow path or the chambers by partially sealing the flow path, the chambers or both the flow path and the chambers by applying external force to the elastic body from outside, as the chambers, the cartridge has two or more mixing chambers each of which is to contain a mixture in a fluid state and the cartridge has two or more ingredient chambers provided for each of the mixing chambers by being connected with the flow path, in each of which an ingredient of the mixture divided in an amount according to a mixing ratio is to be contained.
摘要翻译：公开了一种化学反应盒，其包括作为构造材料的弹性体和流路以及通过形成在筒内部的流路连接的两个或更多个室，并且盒被构造成在流动中移动或阻挡流体物质路径或腔室，通过从外部向弹性体施加外力，通过部分地密封流动路径，腔室或两者的流动路径和腔室，作为腔室，盒具有两个或更多个混合室，每个混合室各自包含流体状态的混合物，并且筒具有通过与流路连接而设置用于每个混合室的两个或更多个成分室，其中每个混合物中的成分按照混合比分配为被包含

5. 发明授权

US07789270B2 Chemical reaction cartridge and method using same 有权
标题翻译：化学反应筒及其使用方法
公开(公告)号：US07789270B2
公开(公告)日：2010-09-07
申请号：US11524948
申请日：2006-09-22
申请人： Takeo Tanaami , Hidetoshi Aoki , Saya Satou , Takeyuki Mogi
发明人： Takeo Tanaami , Hidetoshi Aoki , Saya Satou , Takeyuki Mogi
IPC分类号： B65D35/22
CPC分类号： B01L3/502707 , B01F5/0641 , B01F5/0645 , B01F5/0693 , B01F5/0696 , B01F13/0059 , B01F15/0201 , B01F15/025 , B01J19/0093 , B01J2219/00783 , B01J2219/00833 , B01J2219/00835 , B01J2219/0086 , B01J2219/00889 , B01J2219/00891 , B01L3/5027 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481
摘要： A chemical reaction cartridge enabling chemical reaction to be stably and safely carried out therein, and a method of using the same are provided. A chemical reaction cartridge is provided with a substrate, and an elastic member 2 overlaid on the substrate. A recess of a predetermined shape, depressed toward the top surface of the elastic member is formed in the back surface of the elastic member. The recess creates space between the substrate, and the elastic member, thereby making up a well, for holding the main ingredient of the epoxy adhesive, a flow path communicating with the well, a well for holding a curing agent for the epoxy adhesive, a flow path communicating with the well, a mixing part for mixing the main ingredient with the curing agent, and a discharge path for discharging a mixture. While the chemical reaction cartridge is not in use as yet, the flow paths, and the discharge path are closed, so that the wells are, in effect, in a hermetically sealed condition. The mixing part is made up of a plurality of flow paths intersecting each other. The respective starting ends of the flow paths are each linked with the flow path, or the flow path, and the respective terminals of the flow paths are linked with the discharge path. The terminal of the discharge path communicates with the outside via an end face of the cartridge.
摘要翻译：能够使化学反应稳定且安全地进行的化学反应筒及其使用方法。化学反应盒设置有基板和覆盖在基板上的弹性部件2。在弹性部件的背面形成有朝向弹性部件的上表面凹陷的规定形状的凹部。凹部在基板和弹性部件之间形成空间，从而构成一个孔，用于保持环氧粘合剂的主要成分，与该孔连通的流路，用于保持环氧粘合剂的固化剂的孔，与井连通的流路，用于混合主成分与固化剂的混合部以及排出混合物的排出路径。虽然化学反应盒尚未使用，但是流动路径和排放路径是关闭的，因此实际上这些井处于气密密封状态。混合部分由彼此相交的多个流路组成。流路的各个起始端各自与流路或流路连通，流路的各端子与排出路径连结。排出路径的端子经由筒的端面与外部连通。

6. 发明授权

US08877918B2 Nucleic acid extraction method 有权
标题翻译：核酸提取法
公开(公告)号：US08877918B2
公开(公告)日：2014-11-04
申请号：US13362373
申请日：2012-01-31
申请人： Yoshihiro Ozeki , Akiyo Yamada , Nobuhiro Sasaki , Hitoshi Wake , Takeyuki Mogi , Tomoyuki Taguchi
发明人： Yoshihiro Ozeki , Akiyo Yamada , Nobuhiro Sasaki , Hitoshi Wake , Takeyuki Mogi , Tomoyuki Taguchi
IPC分类号： C07H21/00 , C12N15/10 , C12N1/06 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N1/06 , C12N15/1003 , C12Q2527/101 , C12Q2527/113
摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.
摘要翻译：提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。核酸提取方法包括以下步骤：将细胞悬浮液引入容器，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤加热器加热至设定温度，从而将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃，容器保持密封状态。

7. 发明申请

US20120197008A1 NUCLEIC ACID AND EXTRACTION METHOD 有权
标题翻译：核酸和提取方法
公开(公告)号：US20120197008A1
公开(公告)日：2012-08-02
申请号：US13362373
申请日：2012-01-31
申请人： Yoshihiro Ozeki , Akiyo Yamada , Nobuhiro Sasaki , Hitoshi Wake , Takeyuki Mogi , Tomoyuki Taguchi
发明人： Yoshihiro Ozeki , Akiyo Yamada , Nobuhiro Sasaki , Hitoshi Wake , Takeyuki Mogi , Tomoyuki Taguchi
IPC分类号： C07H1/08
CPC分类号： C12Q1/6806 , C12N1/06 , C12N15/1003 , C12Q2527/101 , C12Q2527/113
摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.
摘要翻译：提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。核酸提取方法包括以下步骤：将细胞悬浮液引入容器中，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤加热器加热到设定温度，由此将容器保持在密封状态下，将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃。

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