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1. 发明申请

US20190335724A1 UROKINASE-TYPE PLASMINOGEN ACTIVATOR TRANSGENIC MOUSE 审中-公开
公开(公告)号：US20190335724A1
公开(公告)日：2019-11-07
申请号：US15934520
申请日：2018-03-23
申请人： TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE , CHUGAI SEIYAKU KABUSHIKI KAISHA , PHOENIXBIO CO., LTD.
发明人： Michinori KOHARA , Koichi JISHAGE , Yosuke KAWASE , Chise MUKAIDANI , Hiroki OSHITA , Satoko HAMAMURA
IPC分类号： A01K67/027 , G01N33/50 , C12N9/72 , C12N15/85 , C12N15/877
摘要： The present invention provides a mouse with liver damage, having a high degree of damage against the mouse's original hepatocytes while having a uPA gene in a heterozygous form, and a method for efficiently preparing the mouse. Specifically, the method for preparing a mouse with liver damage having the uPA gene in a heterozygous form comprises the following steps of: (i) transforming mouse ES cells with a DNA fragment containing a liver-specific promoter/enhancer and cDNA that encodes a urokinase-type plasminogen activator operably linked under the control thereof; (ii) injecting the transformed mouse ES cells obtained in step (i) into a host embryo; (iii) transplanting the host embryo obtained in step (ii) via the injection of the ES cells into the uterus of a surrogate mother mouse, so as to obtain a chimeric mouse; and (iv) crossing the chimeric mice obtained in step (iii), so as to obtain a transgenic mouse in which the DNA fragment is introduced in a heterozygous form.

2. 发明申请

US20140178856A1 POLYNUCLEOTIDE DERIVED FROM NOVEL HEPATITIS C VIRUS STRAIN AND USE THEREOF 审中-公开
标题翻译：从新型丙型肝炎病毒株衍生的多核苷酸及其用途
公开(公告)号：US20140178856A1
公开(公告)日：2014-06-26
申请号：US14078903
申请日：2013-11-13
申请人： PHOENIXBIO CO., LTD. , TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE
发明人： Michinori KOHARA , Masaaki ARAI , Chise MUKAIDANI
IPC分类号： C12Q1/70
CPC分类号： C12Q1/707 , C12N7/00 , C12N2770/24221 , C12N2770/24222 , C12N2770/24243 , C12N2770/24251 , G01N2333/18 , G01N2500/10
摘要： A polynucleotide encoding the amino acid shown in SEQ ID NO:2 or SEQ ID NO: 5, or encoding an amino acid sequence having not less than 98% identity thereto; preferably a polynucleotide comprising replacement of the amino acid corresponding to glutamic acid at position 1202 of SEQ ID NO:2 (position 177 of SEQ ID NO:5) with glycine, replacement of the amino acid corresponding to glutamic acid at position 1056 (position 31 of SEQ ID NO:5) with valine, and replacement of the amino acid corresponding to alanine at position 2199 (position 1174 of SEQ ID NO:5) with threonine.
摘要翻译：编码SEQ ID NO：2或SEQ ID NO：5所示氨基酸的多核苷酸，或编码与其不同于98％同一性的氨基酸序列; 优选包括将SEQ ID NO：2（SEQ ID NO：5的第177位）的1202位相应于谷氨酸的氨基酸与甘氨酸取代的多核苷酸，替换对应于1056位谷氨酸的氨基酸（位置31 SEQ ID NO：5）与缬氨酸的氨基酸取代，并且用苏氨酸置换对应于位置2199（SEQ ID NO：5的位置1174）的丙氨酸。

3. 发明申请

US20170055504A1 HYPERURICEMIA MODEL 审中-公开
标题翻译： HYPERURICEMIA模型
公开(公告)号：US20170055504A1
公开(公告)日：2017-03-02
申请号：US15308116
申请日：2015-05-07
申请人： PHOENIXBIO CO., LTD.
发明人： Masakazu KAKUNI , Yumiko IWASAKI , Chise MUKAIDANI
IPC分类号： A01K67/027 , G01N33/50
CPC分类号： A01K67/0271 , A01K67/0276 , A01K2207/12 , A01K2207/15 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0362 , G01N33/50 , G01N33/5088 , G01N2500/00 , G01N2800/708
摘要： Provided is a non-human animal that is highly practical as a hyperuricenia model, the non-human animal being the following: (a) a non-human animal obtained by producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction; and subsequently administering a purine base-containing substance to the primary chimeric non-human animal, or (b) a non-human animal obtained by producing a serially transplanted chimeric non-human animal via two steps, a first step being a step of producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction, a second step being a step of transplanting the human hepatocytes grown in the body of the primary chimeric non-human animal to an immunodeficient non-human animal with liver dysfunction, the second step being performed one or more times; and subsequently administering a purine base-containing substance to the serially transplanted chimeric non-human animal.
摘要翻译：提供作为高尿酸血症模型非常实用的非人类动物，非人类动物如下：（a）通过将人肝细胞移植到一个或多个第一嵌合非人动物中而获得的非人动物具有肝功能障碍的免疫缺陷型非人动物; 并且随后将所述含嘌呤碱的物质施用于所述一级嵌合非人动物，或（b）通过两步生产连续移植的嵌合非人动物得到的非人动物，第一步是产生通过将肝细胞移植到具有肝功能障碍的免疫缺陷型非人动物中的第一嵌合非人动物，第二步是将生长在初级嵌合非人动物体内的人肝细胞移植到免疫缺陷型非人动物的步骤， - 具有肝功能障碍的人类动物，第二步执行一次或多次; 并随后向连续移植的嵌合非人动物施用含嘌呤碱的物质。

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