专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

US20150119343A1 Production of TSG-6 Protein 审中-公开
标题翻译：生产TSG-6蛋白
公开(公告)号：US20150119343A1
公开(公告)日：2015-04-30
申请号：US14354381
申请日：2012-11-01
申请人： The Texas A & M University System
发明人： Darwin J. Prockop , Jun Watanabe , Dong-Ki Kim , RyangHwa Lee , Hosoon Choi
IPC分类号： C07K14/47 , A61K38/17
CPC分类号： C07K14/4718 , A61K38/1709
摘要： A method of producing a protein or polypeptide, such as, for example, TSG-6 protein, or a biologically active fragment, derivative or analogue thereof, by introducing into mammalian cells a polynucleotide encoding the biologically active protein or polypeptide or biologically active fragment, derivative, or analogue thereof. The cells then are suspended in a protein-free medium that includes at least one agent that suppresses production of hyaluronic acid, hyaluronan, or a salt thereof by the cells. The cells are cultured for a time sufficient to express the biologically active protein or polypeptide or biologically active fragment, derivative or analogue thereof. The biologically active protein or polypeptide, or fragment, derivative, or analogue thereof then is recovered from the cells, such as, for example, by recovering the protein or polypeptide secreted by the cells from the cell culture medium.
摘要翻译：通过将编码生物活性蛋白质或多肽或生物活性片段的多核苷酸引入哺乳动物细胞中来产生蛋白质或多肽例如TSG-6蛋白或其生物活性片段，衍生物或类似物的方法，衍生物或类似物。然后将细胞悬浮在不含蛋白质的培养基中，所述培养基包括至少一种抑制细胞产生透明质酸，透明质酸或其盐的药剂。培养细胞足以表达生物活性蛋白质或多肽或其生物活性片段，衍生物或类似物的时间。然后从细胞中回收生物活性蛋白质或多肽或其片段，衍生物或类似物，例如通过从细胞培养基中回收细胞分泌的蛋白质或多肽。

IPRDB

热门服务

关于我们

友情链接

联系方式